DE69434661D1 - An die periplasmatische Membran gebundenes System zur Ermittlung von Protein-Protein Interaktionen - Google Patents

An die periplasmatische Membran gebundenes System zur Ermittlung von Protein-Protein Interaktionen

Info

Publication number
DE69434661D1
DE69434661D1 DE69434661T DE69434661T DE69434661D1 DE 69434661 D1 DE69434661 D1 DE 69434661D1 DE 69434661 T DE69434661 T DE 69434661T DE 69434661 T DE69434661 T DE 69434661T DE 69434661 D1 DE69434661 D1 DE 69434661D1
Authority
DE
Germany
Prior art keywords
ligand
fusion protein
binding
reporter gene
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69434661T
Other languages
English (en)
Other versions
DE69434661T2 (de
Inventor
Rolf Menzel
Scott T Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Squibb Co
Original Assignee
Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Publication of DE69434661D1 publication Critical patent/DE69434661D1/de
Application granted granted Critical
Publication of DE69434661T2 publication Critical patent/DE69434661T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C07K2319/715—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16 containing a domain for ligand dependent transcriptional activation, e.g. containing a steroid receptor domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
DE69434661T 1993-09-13 1994-09-12 An die periplasmatische Membran gebundenes System zur Ermittlung von Protein-Protein Interaktionen Expired - Fee Related DE69434661T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/121,201 US5521066A (en) 1993-09-13 1993-09-13 Periplasmic membrane-bound system for detecting protein-protein interactions
US121201 1993-09-13

Publications (2)

Publication Number Publication Date
DE69434661D1 true DE69434661D1 (de) 2006-05-11
DE69434661T2 DE69434661T2 (de) 2006-12-07

Family

ID=22395200

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434661T Expired - Fee Related DE69434661T2 (de) 1993-09-13 1994-09-12 An die periplasmatische Membran gebundenes System zur Ermittlung von Protein-Protein Interaktionen

Country Status (7)

Country Link
US (2) US5521066A (de)
EP (1) EP0646644B1 (de)
JP (1) JP3527288B2 (de)
AT (1) ATE320497T1 (de)
CA (1) CA2117612C (de)
DE (1) DE69434661T2 (de)
ES (1) ES2260755T3 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4319296A1 (de) * 1993-06-10 1994-12-15 Behringwerke Ag Genetische Selektion von zur Ligandenbindung befähigten Proteinen mittels Signaltransduktion in Mikroorganismen
EP0750458B1 (de) * 1994-03-03 2003-08-27 Alexion Pharmaceuticals, Inc. Fusionsgene für den inhibitor des terminalen komplements und proteine
US8349602B1 (en) * 1996-04-19 2013-01-08 Xenogen Corporation Biodetectors targeted to specific ligands
US6265174B1 (en) 1997-11-03 2001-07-24 Morphochem, Inc. Methods and compositions for identifying and modulating ctionprotein-interactions
CA2331258C (en) 1998-06-12 2011-09-20 Henry M. Jackson Foundation For The Advancement Of Military Medicine Enhancement of b cell activation and immunoglobulin secretion by co-stimulation of receptors for antigen and ebv gp350/220
CA2335107C (en) * 1998-07-28 2010-01-19 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Leptin-mediated gene-induction
US6210924B1 (en) 1998-08-11 2001-04-03 Amgen Inc. Overexpressing cyclin D 1 in a eukaryotic cell line
JP4756813B2 (ja) * 2000-05-22 2011-08-24 フラームス・インテルウニフェルシタイル・インステイチュート・フォール・ビオテヒノロヒー・ヴェーゼットウェー（ヴェーイーベー・ヴェーゼットウェー） レセプターを用いた相互作用の捕捉法
US7094571B2 (en) * 2000-10-27 2006-08-22 The Board Of Regents Of The University Of Texas System Combinatorial protein library screening by periplasmic expression
US7083945B1 (en) 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
JP4343534B2 (ja) 2001-03-02 2009-10-14 ゲーペーツェー バイオテック アクチェンゲゼルシャフト ３ハイブリッド・アッセイ・システム
EP1578914A2 (de) * 2001-09-12 2005-09-28 Pharmacia & Upjohn Company LLC Menschliche ionenkanäle
WO2003040385A2 (en) * 2001-11-06 2003-05-15 Mcgill University Membrane protein interaction assays
US7611866B2 (en) * 2002-07-15 2009-11-03 Board Of Regents, The University Of Texas System Selection of bacterial inner-membrane anchor polypeptides
CA2560074A1 (en) * 2004-03-18 2005-11-03 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression
CA2585549A1 (en) * 2004-11-18 2006-05-26 Vib Vzw Novel type leptin receptor antagonist

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4774182A (en) * 1984-06-29 1988-09-27 Wisconsin Alumni Research Foundation Partially defective foreign gene for conferring immunity on a biological host
SE8605621D0 (sv) * 1986-12-30 1986-12-30 Biokonsult Ab Heteromultimeric proteins and their manufacture
FR2621597B1 (fr) * 1987-07-24 1992-07-31 Pasteur Institut Procede de clonage et d'expression de genes codant pour des proteines constituant des edifices fonctionnels organises
GB8807683D0 (en) * 1988-03-31 1988-05-05 Ici Plc Regulation of gene expression
WO1991016429A1 (en) * 1990-04-19 1991-10-31 The General Hospital Corporation Protein partner screening assays and uses thereof
AU8059891A (en) * 1990-06-13 1992-01-07 Trustees Of Princeton University, The A (lac) repressor-hsv vp16 chimeric transcriptional activator protein system functioning in transfected mammalian cells
US5219990A (en) * 1991-01-28 1993-06-15 Biogen, Inc. Papillomavirus e2 trans-activation repressors
DE69229222D1 (de) * 1991-03-07 1999-06-24 Bradbury Auswahl spezifischer proteine durch ein biologisches verfahren
DE4319296A1 (de) * 1993-06-10 1994-12-15 Behringwerke Ag Genetische Selektion von zur Ligandenbindung befähigten Proteinen mittels Signaltransduktion in Mikroorganismen

Also Published As

Publication number Publication date
US5521066A (en) 1996-05-28
US5744314A (en) 1998-04-28
EP0646644B1 (de) 2006-03-15
CA2117612C (en) 2008-12-30
JPH07250689A (ja) 1995-10-03
ATE320497T1 (de) 2006-04-15
JP3527288B2 (ja) 2004-05-17
CA2117612A1 (en) 1995-03-14
DE69434661T2 (de) 2006-12-07
ES2260755T3 (es) 2006-11-01
EP0646644A3 (de) 1996-07-10
EP0646644A2 (de) 1995-04-05

Similar Documents

Publication Publication Date Title
DE69434661D1 (de) An die periplasmatische Membran gebundenes System zur Ermittlung von Protein-Protein Interaktionen
Wang et al. The COUP-TFs compose a family of functionally related transcription factors
US20120149870A1 (en) Sequentially arranged streptavidin-binding modules as affinity tags
ATE365325T1 (de) Screening von kombinatorischen peptidbibliotheken zur selektion von peptidliganden zur verwendung in der affinitätsreinigung von zielproteinen
NO2010021I1 (no) Denosumab
NL300153I1 (nl) Voor Factor VIIIC coderende DNA-sequenties en verwante DNA-constructen.
DE69835143D1 (de) Selektion von proteinen mittels rns-protein fusionen
US20160054331A1 (en) Method for the detection and/or enrichment of analyte proteins and/or analyte peptides from a complex protein mixture
DE69321580D1 (de) Car-rezeptoren und verwandte moleküle und entsprechende methoden
ATE372384T1 (de) Protein-tyrosin-phosphatase ptp-s31
DE69634512D1 (de) Traf-inhibitoren
DE69533777D1 (de) Mit retenoid x-rezeptor interagierende polypeptide und verwandte moleküle und verfahren
Zeng et al. Design of inhibitors of Ras–Raf interaction using a computational combinatorial algorithm
ATE462721T1 (de) Breit einsetzbares verfahren zur identifizierung von modulatoren von g-protein gekoppelten rezeptoren
Underwood et al. Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region
DE60219429D1 (de) Biochemisches verfahren und vorrichtung zur bestimmung von eigenschaften von proteinen
Coulter et al. Trypanosoma brucei: characterization of a life cycle stage-specific G-protein
WO2018043629A1 (ja) 非天然型立体構造を形成した抗体に親和性を示すポリペプチド
DE69316388D1 (de) CRF Bindungsproteine
Pacholczyk et al. Epitope and mimotope for an antibody to the Na, K‐ATPase
US11746140B2 (en) Composition comprising recombinant GPlbα receptor protein
Nice et al. Strategies for the identification and purification of ligands for orphan biomolecules
Schröder et al. Identification of a novel platelet-derived neutrophil-chemotactic polypeptide with structural homology to platelet-factor 4
Zaccolo Selection of functional antibodies on the basis of valency

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee