DE69425379D1 - Elongation, amplifikation und sequenzierung unter verwendung eines chimären oligonucleotids - Google Patents

Elongation, amplifikation und sequenzierung unter verwendung eines chimären oligonucleotids

Info

Publication number
DE69425379D1
DE69425379D1 DE69425379T DE69425379T DE69425379D1 DE 69425379 D1 DE69425379 D1 DE 69425379D1 DE 69425379 T DE69425379 T DE 69425379T DE 69425379 T DE69425379 T DE 69425379T DE 69425379 D1 DE69425379 D1 DE 69425379D1
Authority
DE
Germany
Prior art keywords
oligonucleotid
chimeric
sequencing
elongation
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE69425379T
Other languages
English (en)
Other versions
DE69425379T2 (de
Inventor
Alan Reeve
Tom Brown
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
Amersham Pharmacia Biotech UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8214551&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69425379(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amersham Pharmacia Biotech UK Ltd filed Critical Amersham Pharmacia Biotech UK Ltd
Publication of DE69425379D1 publication Critical patent/DE69425379D1/de
Application granted granted Critical
Publication of DE69425379T2 publication Critical patent/DE69425379T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69425379T 1993-09-21 1994-09-21 Elongation, amplifikation und sequenzierung unter verwendung eines chimären oligonucleotids Revoked DE69425379T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93307455 1993-09-21
PCT/GB1994/002053 WO1995008556A1 (en) 1993-09-21 1994-09-21 Reagents comprising chimeric molecules of nucleic acids and nucleic acid analogs

Publications (2)

Publication Number Publication Date
DE69425379D1 true DE69425379D1 (de) 2000-08-31
DE69425379T2 DE69425379T2 (de) 2000-11-30

Family

ID=8214551

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69425379T Revoked DE69425379T2 (de) 1993-09-21 1994-09-21 Elongation, amplifikation und sequenzierung unter verwendung eines chimären oligonucleotids

Country Status (6)

Country Link
US (1) US20020068275A1 (de)
EP (1) EP0720615B1 (de)
AU (1) AU7661794A (de)
DE (1) DE69425379T2 (de)
DK (1) DK0720615T3 (de)
WO (1) WO1995008556A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5700922A (en) * 1991-12-24 1997-12-23 Isis Pharmaceuticals, Inc. PNA-DNA-PNA chimeric macromolecules
US6277603B1 (en) 1991-12-24 2001-08-21 Isis Pharmaceuticals, Inc. PNA-DNA-PNA chimeric macromolecules
US6133444A (en) 1993-12-22 2000-10-17 Perseptive Biosystems, Inc. Synthons for the synthesis and deprotection of peptide nucleic acids under mild conditions
EP0760008A1 (de) * 1994-05-19 1997-03-05 Dako A/S Pna-sonden zum nachweis von neisseria gonorrhoeae und chlamydia trachomatis
US5629178A (en) * 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
US6465650B1 (en) 1995-03-13 2002-10-15 Aventis Pharma Deutschland Gmbh Substituted N-ethylglycine derivatives for preparing PNA and PNA/DNA hybrids
WO1996036734A1 (en) * 1995-05-18 1996-11-21 Abbott Laboratories Polymeric peptide probes and uses thereof
EP0840741B1 (de) * 1995-06-07 2004-04-28 Perseptive Biosystems, Inc. Pna-dna-chimäre und pna-synthone zu deren herstellung
US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
DE19637339A1 (de) * 1996-09-13 1998-03-19 Hoechst Ag Verfahren zur Amplifikation von Nukleinsäuren
CA2218439A1 (en) * 1996-12-21 1998-06-21 Henrik Orum Method of identifying a nucleic acid using triple helix formation of adjacently annealed probes
ATE413881T1 (de) 1997-08-08 2008-11-15 Celmed Oncology Usa Inc Verfahren und zubereitungen um resistenz gegen biologische oder chemische therapien zu überwinden
US6043070A (en) * 1997-08-29 2000-03-28 The Perkin-Elmer Corporation Phosphoramidate-phosphodiester oligonucleotide chimera as primers
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
US6054568A (en) * 1998-01-16 2000-04-25 The Perkin-Elmer Corporation Nucleobase oligomers
US6030997A (en) * 1998-01-21 2000-02-29 Eilat; Eran Acid labile prodrugs
US7462605B2 (en) * 1998-01-23 2008-12-09 Celmed Oncology (Usa), Inc. Phosphoramidate compounds and methods of use
EP1167972B1 (de) 1998-01-23 2017-03-08 Kiadis Pharma Intellectual Property B.V. Enzymkatalisierte therapeutische Agenzien
EP1073766A1 (de) 1998-04-29 2001-02-07 Trustees Of Boston University Methoden und zusammensetzungen betreffend pd-loops
US6683061B1 (en) 1999-07-22 2004-01-27 Newbiotics, Inc. Enzyme catalyzed therapeutic activation
CN1391486A (zh) * 1999-07-22 2003-01-15 新生物公司 治疗对治疗有抗性的肿瘤的方法
US6316230B1 (en) * 1999-08-13 2001-11-13 Applera Corporation Polymerase extension at 3′ terminus of PNA-DNA chimera
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
CA2441350A1 (en) 2001-01-19 2002-07-25 Newbiotics, Inc. Methods to treat autoimmune and inflammatory conditions
US9677123B2 (en) 2006-03-15 2017-06-13 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
US20080131880A1 (en) * 2006-11-22 2008-06-05 Bortolin Laura T PNA-DNA oligomers and methods of use thereof
JP7220439B1 (ja) * 2022-03-14 2023-02-10 国立大学法人東北大学 キメラ分子の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0224126A3 (de) * 1985-11-25 1989-02-01 The University of Calgary Kovalent gekoppelte komplementäre Oligodeoxynukleotide zur Verwendung als "Primer Linkers" bei Sequenzierung von Nukleinsäuren
CA1337639C (en) * 1989-08-01 1995-11-28 Joseph Eugene Celebuski Dna probe assay using neutrally charged probe strands
DE4110085A1 (de) * 1991-03-27 1992-10-01 Boehringer Ingelheim Int 2'-o-alkyl-oligoribonukleotide, verfahren zu deren herstellung und deren verwendung als antisense-oligonukleotide
DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
AU2844792A (en) * 1991-12-11 1993-06-17 Becton Dickinson & Company Probes to chlamydia trachomatis

Also Published As

Publication number Publication date
EP0720615B1 (de) 2000-07-26
WO1995008556A1 (en) 1995-03-30
EP0720615A1 (de) 1996-07-10
AU7661794A (en) 1995-04-10
DE69425379T2 (de) 2000-11-30
DK0720615T3 (da) 2000-10-30
US20020068275A1 (en) 2002-06-06

Similar Documents

Publication Publication Date Title
DE69425379D1 (de) Elongation, amplifikation und sequenzierung unter verwendung eines chimären oligonucleotids
DE59907464D1 (de) 1,3-oxazolin- und 1,3-thiazolin-derivate, verfahren zu ihrer herstellung und ihre verwendung als schädlingsbekämpfungsmittel
NO307513B1 (no) 2,8-disubstituert chinazolinon og anvendelse derav
DE69632324D1 (de) Pna-dna-chimäre und pna-synthone zu deren herstellung
ZA964646B (en) Substituted N-(indole-2-Carbonyl)-ß-alanimamides and derivatives as antidiabetic agents.
DE59506908D1 (de) Oxidationskatalysator, und oxidationsverfahren unter verwendung des oxidationskatalysators
DE69630636D1 (de) Wasserdispergierbares Blatt, und Zigarette unter Verwendung desselben
DK0771789T3 (da) 1 alpha, 26-dihydroxy-D-homo-vitamin D3
DE69406733D1 (de) Wässrige Überzugszusammensetzung und Überzugsverfahren unter Verwendung selbiger
DE69417432D1 (de) Retroreflektierendes blattmaterial,verfahren zu seiner herstellung und verwendung
DK0721457T3 (da) 2',5'-Oligoadenylat-2',3'-cyclophosphater og 2',5'-oligo-adenylater med antipapillomavirusaktivitet
NO301598B1 (no) Martensittisk rustfritt stål
DE69822097D1 (de) Dehnungsaufnehmer und dessen Verwendung
DE69432756D1 (de) 1,3-oxazin-4-on-derivate, verfahren und zwischenprodukte zu deren herstellung und ihre verwendung als herbizide
FI945164A0 (fi) Biologisesti aktiivisesti ureidojohdannaiset, jotka ovat käyttökelpoisia antimetastaattisina aineina
DE69635221D1 (de) Pflanzenpromoter und seine Verwendung
HK1032078A1 (en) Austentic stainless steel including columbium
DE59604539D1 (de) Amperometrischer Zweielektrodensensor.
DE59405241D1 (de) Sauerstoffmessfühler
DE59509781D1 (de) Alkylsulfinyl- und alkylsulfonyl-1,2,4-thiadiazolyloxyacetamide sowie ihre verwendung als herbizide
RU96112258A (ru) Способ усиления солнцезащитной активности фотозащитных агентов
FI953791A0 (fi) Ravirattaat
FI960789A (fi) Kestäviä teräsvanteita käytettäviksi korkeissa lämpötiloissa
DE69629767D1 (de) Promoter und dessen Verwendung zum Expressionsverfahren
ATA94295A (de) Einrichtung zum ausgleich eines momentes

Legal Events

Date Code Title Description
8363 Opposition against the patent
8331 Complete revocation