DE69332893T2 - Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae) - Google Patents

Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae)

Info

Publication number
DE69332893T2
DE69332893T2 DE69332893T DE69332893T DE69332893T2 DE 69332893 T2 DE69332893 T2 DE 69332893T2 DE 69332893 T DE69332893 T DE 69332893T DE 69332893 T DE69332893 T DE 69332893T DE 69332893 T2 DE69332893 T2 DE 69332893T2
Authority
DE
Germany
Prior art keywords
recombinant
proteins
increasing
production
saccharomyces cerevisiae
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69332893T
Other languages
English (en)
Other versions
DE69332893D1 (de
Inventor
Michael F Tuite
Robert B Freedman
D Schultz
W Ellis
Z Markus
L Montgomery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Canterbury
Merck Sharp and Dohme LLC
Original Assignee
University of Kent at Canterbury
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Kent at Canterbury, Merck and Co Inc filed Critical University of Kent at Canterbury
Application granted granted Critical
Publication of DE69332893D1 publication Critical patent/DE69332893D1/de
Publication of DE69332893T2 publication Critical patent/DE69332893T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69332893T 1992-06-12 1993-06-02 Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae) Expired - Lifetime DE69332893T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/901,713 US6291205B1 (en) 1992-06-12 1992-06-12 Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
PCT/US1993/005318 WO1993025676A1 (en) 1992-06-12 1993-06-02 METHOD FOR INCREASING PRODUCTION OF DISULFIDE BONDED RECOMBINANT PROTEINS BY $i(SACCHAROMYCES CEREVISIAE)

Publications (2)

Publication Number Publication Date
DE69332893D1 DE69332893D1 (de) 2003-05-22
DE69332893T2 true DE69332893T2 (de) 2003-12-24

Family

ID=25414688

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69332893T Expired - Lifetime DE69332893T2 (de) 1992-06-12 1993-06-02 Methode zur erhöhung der produktion von rekombinanten disulfidverknüpften-proteinen in (saccharomyces cerevisiae)

Country Status (12)

Country Link
US (1) US6291205B1 (de)
EP (1) EP0746611B1 (de)
JP (3) JP3953094B2 (de)
KR (1) KR0171205B1 (de)
AT (1) ATE237684T1 (de)
AU (1) AU679448B2 (de)
CA (1) CA2137142C (de)
DE (1) DE69332893T2 (de)
DK (1) DK0746611T3 (de)
ES (1) ES2194848T3 (de)
NZ (1) NZ253873A (de)
WO (1) WO1993025676A1 (de)

Families Citing this family (40)

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HU218419B (hu) * 1992-03-06 2000-08-28 E.R. Squibb And Sons, Inc. Mikroszomális triglicerid transzfer protein (MTP) nagy molekulatömegű alegységének rekombináns úton történő előállítására és a protein és inhibitorainak kimutatására szolgáló eljárások
DK76893D0 (de) 1993-06-28 1993-06-28 Novo Nordisk As
EP0635574B1 (de) * 1993-07-23 2003-04-23 Dsm N.V. Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
WO1997038123A1 (en) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US5965426A (en) * 1996-09-04 1999-10-12 Suntory Limited Protein disulfide isomerase gene derived from strain of methylotrophic yeast
GB9703406D0 (en) * 1997-02-19 1997-04-09 Chiron Spa Expression of heterologous proteins
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
US6521421B1 (en) 1997-07-16 2003-02-18 Genencor International, Inc. Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same
DK0990039T3 (da) * 1997-07-16 2006-01-30 Genencor Int Forögelse af produktionen af proteiner i grampositive mikroorganismer
AU2001241125A1 (en) * 2000-03-14 2001-09-24 Itoham Foods Inc. Process for producing polypeptide having disulfide bond
WO2003062375A2 (en) * 2001-12-19 2003-07-31 Genentech, Inc. Stabilizing polypeptides which have been exposed to urea
US20040203132A1 (en) 2003-02-28 2004-10-14 Cognetix, Inc. Conus protein disulfide isomerase
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
US20110034396A1 (en) * 2005-09-28 2011-02-10 Biovascular, Inc. Methods and compositions for inhibiting cell migration and treatment of inflammatory conditions
JP2009510105A (ja) * 2005-09-28 2009-03-12 バイオバスキュラー インコーポレーティッド 血小板および細胞の接着、細胞移動、および炎症を阻止するための方法および組成物
JP2013009685A (ja) * 2005-11-24 2013-01-17 National Institute Of Advanced Industrial Science & Technology 高効率分泌シグナルペプチド及びそれらを利用したタンパク質発現系
EP2041280B1 (de) * 2006-06-20 2016-08-10 Mogam Biotechnology Research Institute Verfahren zur verbesserung der sekretionseffizienz eines rekombinanten fremdproteins im hefeexpressionssystem
FR2919062B1 (fr) * 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'aminoacylase 1 pour le diagnostic in vitro du cancer colorectal.
FR2919065B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'apolipoproteine ai pour le diagnostic in vitro du cancer colorectal
FR2919061B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de la plastine-i pour le diagnostic in vitro du cancer colorectal.
FR2919060B1 (fr) * 2007-07-19 2012-11-30 Biomerieux Sa Procede de dosage de l'ezrine pour le diagnostic in vitro du cancer colorectal.
FR2919064B1 (fr) * 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage de l'apolipoproteine all pour le diagnostic in vitro du cancer colorectal
FR2919063B1 (fr) 2007-07-19 2009-10-02 Biomerieux Sa Procede de dosage du leucocyte elastase inhibitor pour le diagnostic in vitro du cancer colorectal.
WO2009019368A2 (fr) * 2007-07-19 2009-02-12 bioMérieux Procede de dosage de la liver fatty acid-binding protein, de l'ace et du ca19-9 pour le diagnostic in vitro du cancer colorectal
ES2537806T5 (es) † 2007-10-31 2018-10-09 Daiichi Sankyo Company, Limited Método para la secreción y producción de alto nivel de proteína
EP2245151B1 (de) * 2008-02-20 2014-12-24 GlycoFi, Inc. Vektoren und hefestämme für die proteinproduktion
FR2933773B1 (fr) * 2008-07-10 2013-02-15 Biomerieux Sa Procede de dosage de la proteine disulfide isomerase pour le diagnostic in vitro du cancer colorectal
EP2258854A1 (de) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotische Wirtszelle mit einem Ausdrucksverstärker
EP2565262A1 (de) 2011-08-31 2013-03-06 VTU Holding GmbH Protein-Expression
RU2663587C2 (ru) 2013-03-06 2018-08-07 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Клетки-хозяева и способы использования
WO2015158800A1 (en) 2014-04-17 2015-10-22 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
CA2948369C (en) 2014-04-17 2023-09-26 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
EP3877552B1 (de) 2018-11-08 2023-02-22 Sutro Biopharma, Inc. E. coli-stämme mit einem oxidativen zytoplasma
US20210340586A1 (en) 2020-04-30 2021-11-04 Sutro Biopharma, Inc. Methods of Producing Full-Length Antibodies Using E. coli

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005816A1 (en) * 1987-01-28 1988-08-11 Takeda Chemical Industries, Ltd. Polypeptide
IL86455A0 (en) 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
JPH0638771A (ja) * 1990-10-31 1994-02-15 Tonen Corp ヒトプロテインジスルフィドイソメラーゼ遺伝子の発現方法および該遺伝子との共発現によるポリペプチドの製造方法
JPH05317037A (ja) * 1991-10-30 1993-12-03 Tonen Corp 酵母ｅｒｄ２遺伝子とそのリガンドとしての小胞体局在蛋白質をコードする遺伝子とを含む共発現系及びそれを利用する有用ポリペプチドの製造法
EP0509841A3 (en) * 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen

Also Published As

Publication number Publication date
CA2137142C (en) 2001-09-25
JP2004041210A (ja) 2004-02-12
EP0746611A4 (de) 1997-03-19
EP0746611B1 (de) 2003-04-16
JPH07508881A (ja) 1995-10-05
CA2137142A1 (en) 1993-12-23
DK0746611T3 (da) 2003-05-19
KR0171205B1 (en) 1999-02-01
US6291205B1 (en) 2001-09-18
JP3953094B2 (ja) 2007-08-01
NZ253873A (en) 1995-10-26
ES2194848T3 (es) 2003-12-01
JP2008271976A (ja) 2008-11-13
DE69332893D1 (de) 2003-05-22
AU679448B2 (en) 1997-07-03
JP4148852B2 (ja) 2008-09-10
ATE237684T1 (de) 2003-05-15
WO1993025676A1 (en) 1993-12-23
EP0746611A1 (de) 1996-12-11
JP4562786B2 (ja) 2010-10-13
AU4527493A (en) 1994-01-04

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Owner name: MERCK SHARP & DOHME CORP., NEW JERSEY, N.J., US

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