DE69232097D1 - C-terminale peptidsequenzierung unter verwendung von diphenylphosphoroisothiocyanidat und pyridin - Google Patents

C-terminale peptidsequenzierung unter verwendung von diphenylphosphoroisothiocyanidat und pyridin

Info

Publication number
DE69232097D1
DE69232097D1 DE69232097T DE69232097T DE69232097D1 DE 69232097 D1 DE69232097 D1 DE 69232097D1 DE 69232097 T DE69232097 T DE 69232097T DE 69232097 T DE69232097 T DE 69232097T DE 69232097 D1 DE69232097 D1 DE 69232097D1
Authority
DE
Germany
Prior art keywords
diphenylphosphoroisothiocyanidate
pyridine
terminal peptide
peptide sequencing
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69232097T
Other languages
English (en)
Other versions
DE69232097T2 (de
Inventor
M Bailey
E Shively
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
City of Hope
Original Assignee
City of Hope
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by City of Hope filed Critical City of Hope
Publication of DE69232097D1 publication Critical patent/DE69232097D1/de
Application granted granted Critical
Publication of DE69232097T2 publication Critical patent/DE69232097T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6821—Sequencing of polypeptides involving C-terminal degradation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
DE69232097T 1991-12-03 1992-02-07 C-terminale peptidsequenzierung unter verwendung von diphenylphosphoroisothiocyanidat und pyridin Expired - Fee Related DE69232097T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/801,944 US5180807A (en) 1991-12-03 1991-12-03 C-terminal peptide or protein sequencing reagent and method
PCT/US1992/001009 WO1993011436A1 (en) 1991-12-03 1992-02-07 C-terminal peptide sequencing using diphenyl phosphoroisothiocyanatidate and pyridine

Publications (2)

Publication Number Publication Date
DE69232097D1 true DE69232097D1 (de) 2001-11-08
DE69232097T2 DE69232097T2 (de) 2002-07-11

Family

ID=25182418

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69232097T Expired - Fee Related DE69232097T2 (de) 1991-12-03 1992-02-07 C-terminale peptidsequenzierung unter verwendung von diphenylphosphoroisothiocyanidat und pyridin

Country Status (7)

Country Link
US (1) US5180807A (de)
EP (1) EP0575463B1 (de)
JP (1) JP3251941B2 (de)
AU (1) AU653020B2 (de)
CA (1) CA2101449A1 (de)
DE (1) DE69232097T2 (de)
WO (1) WO1993011436A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5254475A (en) * 1990-08-13 1993-10-19 City Of Hope Sequential C-terminal degradation of peptides and proteins
US5304497A (en) * 1991-10-15 1994-04-19 Applied Biosystems, Inc. Method of forming N-protected amino acid thiohydantoins
US5432092A (en) * 1991-12-03 1995-07-11 City Of Hope C-terminal peptide sequencing using diphenyl phosphoroisothiocyanatidate and pyridine
WO1993019082A1 (en) * 1992-03-25 1993-09-30 Garvan Institute Of Medical Research Method for preparation of amino acid thiohydantoins
US5665603A (en) * 1993-07-26 1997-09-09 The Perkin-Elmer Corporation Thiohydantoin formation and selective modification of the carboxy terminus of an aspartic acid- and/or glutamic acid-containing protein
AU682343B2 (en) * 1994-02-15 1997-10-02 City Of Hope C-terminal sequencing of peptides which may include proline
US5468843A (en) * 1994-09-08 1995-11-21 Perkin-Elmer Acetic anhydride activation for C-terminal protein sequencing
US5641685A (en) * 1995-04-24 1997-06-24 Smithkline Beecham Corporation Method carboxy terminal protein or peptide sequencing
JP3124507B2 (ja) * 1996-05-24 2001-01-15 セイコーインスツルメンツ株式会社 タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法
DE69733349T2 (de) * 1997-09-08 2006-03-23 Seiko Instruments Inc. Verfahren zur Bestimmung der Sequenz von Aminosäuren von Proteinen oder Peptiden, beginnend mit dem Carboxylende

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0217634B1 (de) * 1985-09-26 1992-06-10 Beckman Research Institute of the City of Hope Sequenzierung von Peptiden
US5041388A (en) * 1989-12-21 1991-08-20 Applied Biosystems, Inc. C-terminal peptide sequencing, activated support and reagent system therefor, and method of producing the activated support
US5049507A (en) * 1989-12-21 1991-09-17 Applied Biosystems, Inc. Method of C-terminal peptide sequencing

Also Published As

Publication number Publication date
EP0575463A4 (en) 1996-06-12
JPH06504378A (ja) 1994-05-19
AU653020B2 (en) 1994-09-15
US5180807A (en) 1993-01-19
AU1530892A (en) 1993-06-28
JP3251941B2 (ja) 2002-01-28
DE69232097T2 (de) 2002-07-11
EP0575463A1 (de) 1993-12-29
WO1993011436A1 (en) 1993-06-10
CA2101449A1 (en) 1993-06-04
EP0575463B1 (de) 2001-10-04

Similar Documents

Publication Publication Date Title
DE3788626D1 (de) Nor-Statin und Nor-Cyclostatin enthaltende Polypeptide.
DE68910713D1 (de) Isolierung, Reinigung, Charakterisierung, Klonierung und Sequenzierung von N-alpha-Acetyltransferase.
DE3672924D1 (de) Cystein verminderte muteine von biologisch aktiven menschlichen tnf-proteinen.
DE69220382D1 (de) Verwendung von Peptidderivaten
DK496486A (da) Polypeptid
DE4392789T1 (de) System zum Behandeln von Übergangszonenmaterialien
DK162890D0 (da) Polypeptid
DE69232097D1 (de) C-terminale peptidsequenzierung unter verwendung von diphenylphosphoroisothiocyanidat und pyridin
DK551287D0 (da) Polypeptid
DE3825172A1 (de) Mittel zur bekaempfung von floehen
DK197989A (da) Peptidanaloger
HU9202821D0 (en) Method for cloning and producing polypeptides constituting glycoprotein ib binding domain of human von willebrand factor
DE59308677D1 (de) Regelung von Konzentrationen bei thermischen Trennverfahren
NO842682L (no) Polypeptid og dna-sekvens samt vektor
DE19681207T1 (de) Therapeutische Verfahren und Zubereitungen unter Verwendung von Rubidiumionen
FI955000A0 (fi) Menetelmä polypeptidin läsnäolon paljastamiseksi ja sen muuttamiseksi
KR900700503A (ko) 펩티드
DE69232771T2 (de) Peptide als antagonisten von endothelin
DE3883352D1 (de) Muramylpeptid-derivate und ihre verwendung.
DE69228669T2 (de) Proteinsequenzierung
DK284489A (da) Fusionsproteiner
DK354989A (da) Modificerede proteiner
ATA333085A (de) Verfahren zur absicherung von geologischen formationen und bohrungen zur durchfuehrung des verfahrens
FI951573A (fi) Selkärangattomien tuholaisten kontrolloimiseen käyttökelpoinen polypeptidi
BR1100729A (pt) Polipeptìdeo modificado

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee