DE69231940D1 - Rekombinant DNS kodierend für eine Endochitinase - Google Patents

Rekombinant DNS kodierend für eine Endochitinase

Info

Publication number
DE69231940D1
DE69231940D1 DE69231940T DE69231940T DE69231940D1 DE 69231940 D1 DE69231940 D1 DE 69231940D1 DE 69231940 T DE69231940 T DE 69231940T DE 69231940 T DE69231940 T DE 69231940T DE 69231940 D1 DE69231940 D1 DE 69231940D1
Authority
DE
Germany
Prior art keywords
recombinant dna
endochitinase
dna coding
sequence
homology
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69231940T
Other languages
English (en)
Other versions
DE69231940T2 (de
Inventor
Pierre-Louis Blaiseau
Richard Legoux
Jean-Jacques Leguay
Michel Schneider
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogemma SAS
Original Assignee
Biogemma SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogemma SAS filed Critical Biogemma SAS
Application granted granted Critical
Publication of DE69231940D1 publication Critical patent/DE69231940D1/de
Publication of DE69231940T2 publication Critical patent/DE69231940T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69231940T 1991-09-06 1992-09-04 Rekombinant DNS kodierend für eine Endochitinase Expired - Fee Related DE69231940T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR919111072A FR2681076B1 (fr) 1991-09-06 1991-09-06 Adn recombinant codant pour une proteine a activite endochitinase.

Publications (2)

Publication Number Publication Date
DE69231940D1 true DE69231940D1 (de) 2001-08-23
DE69231940T2 DE69231940T2 (de) 2002-04-04

Family

ID=9416728

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69231940T Expired - Fee Related DE69231940T2 (de) 1991-09-06 1992-09-04 Rekombinant DNS kodierend für eine Endochitinase

Country Status (9)

Country Link
US (1) US5446138A (de)
EP (1) EP0531218B1 (de)
JP (1) JPH0638762A (de)
AT (1) ATE203272T1 (de)
AU (1) AU2280192A (de)
CA (1) CA2077590C (de)
DE (1) DE69231940T2 (de)
FR (1) FR2681076B1 (de)
NZ (1) NZ244244A (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6512166B1 (en) 1991-06-17 2003-01-28 Cornell Research Foundation, Inc. Combinations of fungal cell wall degrading enzyme and fungal cell membrane affecting compound
US6020540A (en) * 1993-04-14 2000-02-01 Cornell Research Foundation, Inc. Gene encoding endochitinase
EP0639642A1 (de) * 1993-08-17 1995-02-22 Mogen International N.V. Chitinase, dafür kodierende DNA und diese enthaltende Pflanzen
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
EP1559776A3 (de) * 1994-06-30 2006-01-11 Novozymes Biotech, Inc. Nicht-toxisches, nicht-toxigenes, nicht-pathogenes Fusarium Expressionssystem und darin zu verwendende Promotoren und Terminatoren
US6455686B1 (en) * 1998-04-17 2002-09-24 Heska Corporation Dermatophagoides nucleic acid molecules, proteins and uses thereof
US6521435B1 (en) * 1999-08-30 2003-02-18 The United States Of America As Represented By The Secretary Of Agriculture Nucleic acid sequences encoding cell wall-degrading enzymes and use to engineer resistance to Fusarium and other pathogens
WO2001046387A1 (en) * 1999-12-23 2001-06-28 Cornell Research Foundation, Inc. Effect on endochitinase and chitobiosidase and their encoding genes on plant growth and development
US9049814B2 (en) * 2007-02-23 2015-06-09 Vamtech, Llc Coated seeds and methods of making coated seeds
CN111763665B (zh) * 2019-04-01 2022-08-12 江苏师范大学 一种高活性甘薯几丁质酶的制备方法及其在制备植物病原真菌抗菌剂方面的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
EP0590301A2 (de) * 1986-07-25 1994-04-06 Louisiana State University and Agricultural and Mechanical College Verfahren zum Schutz von Pflanzen gegen Krankheit und Befall, durch Geneinbau und diese Gene enthaltende Vektoren und Pflanzenzellen
JPS63313590A (ja) * 1987-03-10 1988-12-21 バイオテクニカ・インターナショナル・インコーポレーテッド キチナーゼ産生と分必べクター
AU4828490A (en) * 1988-12-16 1990-07-10 E.I. Du Pont De Nemours And Company Overexpression of chitinase in transgenic plants
KR910014790A (ko) * 1990-01-10 1991-08-31 한태희 랩탑 컴퓨터의 기능선택방법 및 인터페이스회로

Also Published As

Publication number Publication date
JPH0638762A (ja) 1994-02-15
CA2077590C (en) 2003-01-28
ATE203272T1 (de) 2001-08-15
FR2681076A1 (fr) 1993-03-12
US5446138A (en) 1995-08-29
NZ244244A (en) 1995-03-28
DE69231940T2 (de) 2002-04-04
FR2681076B1 (fr) 1994-11-18
AU2280192A (en) 1993-03-11
EP0531218B1 (de) 2001-07-18
CA2077590A1 (en) 1993-03-07
EP0531218A1 (de) 1993-03-10

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: GROSSE, BOCKHORNI, SCHUMACHER, 81476 MUENCHEN

8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: BOCKHORNI & KOLLEGEN, 80687 MUENCHEN

8339 Ceased/non-payment of the annual fee