DE69228055T2 - Ein gewebekulturverfahren für haut im natürlichen zustand - Google Patents

Ein gewebekulturverfahren für haut im natürlichen zustand

Info

Publication number
DE69228055T2
DE69228055T2 DE69228055T DE69228055T DE69228055T2 DE 69228055 T2 DE69228055 T2 DE 69228055T2 DE 69228055 T DE69228055 T DE 69228055T DE 69228055 T DE69228055 T DE 69228055T DE 69228055 T2 DE69228055 T2 DE 69228055T2
Authority
DE
Germany
Prior art keywords
skin
disclosed
matrix
medium
collagen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69228055T
Other languages
English (en)
Other versions
DE69228055D1 (de
Inventor
Lingna Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anticancer Inc
Original Assignee
Anticancer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anticancer Inc filed Critical Anticancer Inc
Application granted granted Critical
Publication of DE69228055D1 publication Critical patent/DE69228055D1/de
Publication of DE69228055T2 publication Critical patent/DE69228055T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/092—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Dermatology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE69228055T 1991-02-28 1992-02-28 Ein gewebekulturverfahren für haut im natürlichen zustand Expired - Lifetime DE69228055T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US66223991A 1991-02-28 1991-02-28
PCT/US1992/001571 WO1992015700A1 (en) 1991-02-28 1992-02-28 A native-state histoculturing method for skin

Publications (2)

Publication Number Publication Date
DE69228055D1 DE69228055D1 (de) 1999-02-11
DE69228055T2 true DE69228055T2 (de) 1999-05-20

Family

ID=24656946

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69228055T Expired - Lifetime DE69228055T2 (de) 1991-02-28 1992-02-28 Ein gewebekulturverfahren für haut im natürlichen zustand

Country Status (6)

Country Link
US (1) US5849579A (de)
EP (1) EP0573606B1 (de)
JP (1) JP2950519B2 (de)
AT (1) ATE175242T1 (de)
DE (1) DE69228055T2 (de)
WO (1) WO1992015700A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399380B1 (en) * 1991-02-28 2002-06-04 Anti-Cancer, Inc. Native-state histoculturing skin extracellular support
US5741701A (en) * 1994-04-25 1998-04-21 Becton, Dickinson And Company Cell culture substrates and methods of use
USRE40209E1 (en) 1994-06-13 2008-04-01 Matsushita Electric Industrial Co., Ltd. Cell potential measurement apparatus having a plurality of microelectrodes
US5563067A (en) 1994-06-13 1996-10-08 Matsushita Electric Industrial Co., Ltd. Cell potential measurement apparatus having a plurality of microelectrodes
US6890762B1 (en) 1996-01-24 2005-05-10 Matsushita Technical Information Services Co., Ltd. Method for measuring physiocochemical properties of tissues of cells, method for examining chemicals, and apparatus therefor
WO1997027318A1 (fr) * 1996-01-24 1997-07-31 Matsushita Electric Industrial Co., Ltd. Procede de mesure de proprietes physico-chimiques de tissus ou de cellules, procede d'examen de substances chimiques et equipement correspondant
US6551772B1 (en) 1996-11-27 2003-04-22 Anticancer, Inc. Model for viral infection and immune response
DE10100122A1 (de) * 2001-01-03 2002-07-11 Henkel Kgaa Verfahren zur Bestimmung der Hautalterung in vitro
US20020182112A1 (en) * 2001-04-30 2002-12-05 Unilever Home & Personal Care Usa, Division Of Conopco, Inc. In vivo method for measuring binding of chemical actives to skin or specific constituents of skin
AU2003298543C1 (en) 2002-08-09 2011-06-02 Bioresource International, Inc. Methods and compositions for improving growth of meat-type poultry
AU2003294267B2 (en) 2002-11-12 2009-01-08 Anticancer, Inc. Expression profiling based on histocultures
FR2847345B1 (fr) * 2002-11-18 2005-02-18 Oreal Procede de criblage et/ou d'evaluation de l'activite anti-ride de produits
GB0512214D0 (en) * 2005-06-15 2005-07-27 Capsant Neurotechnologies Ltd Method
DE102007026639A1 (de) 2007-06-06 2008-12-11 Stefan-Andreas Ulrich Industrielle Fertigung tierischer Lederhautsubstitute für die Lederproduktion aus etablierten Epithelzelllinien und/oder primären Epithelzellkulturen, wie vorzugsweise Fibroplasten, Melanozyten, Keratiozyten sowie deren Verfahren und Vorrichtungen
CA2658074C (fr) * 2008-03-17 2017-11-07 L'oreal Equivalent de peau pigmentee fonctionnelle
EP3321371A1 (de) * 2016-11-09 2018-05-16 Ying Zhang Neuartige verfahren zur schnellen arzneimittelanfälligkeitsprüfung und arzneimittel-screens für wachsende und nichtwachsende zellen von prokaryotischem und eukaryotischem ursprung
CA3055767A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Methods for evaluating tumor cell spheroids using 3d microfluidic cell culture device

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4508819A (en) * 1971-04-22 1985-04-02 Bio-Response, Inc. Method for culturing cells or tissues
FR2400063A1 (fr) * 1977-08-08 1979-03-09 Pasteur Institut Procede d'obtention de supports pour cultures cellulaires et supports obtenus
DE3402927A1 (de) * 1984-01-28 1985-08-08 Pfeifer & Langen, 5000 Köln Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen
GB8604360D0 (en) * 1986-02-21 1986-03-26 Univ Dundee Stimulation of hair growth
FR2597499A1 (fr) * 1986-04-18 1987-10-23 Merieux Inst Procede de culture microbiologique sur supports de collagene, supports pour ce procede et produits obtenus
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
GB8721018D0 (en) * 1987-09-07 1987-10-14 Alcan Int Ltd Porous inorganic membrane support
US5015584A (en) * 1987-10-14 1991-05-14 Board Of Regents, The University Of Texas System Epidermal graft system
US4994388A (en) * 1988-04-15 1991-02-19 Solohill Engineering, Inc. Collagen-coated polystyrene microcarrier beads
US4940853A (en) * 1988-07-22 1990-07-10 Vandenburgh Herman H Method for growing tissue specimens in vitro
US4996154A (en) * 1989-05-04 1991-02-26 Millipore Corporation Method for growing cellular tissue

Also Published As

Publication number Publication date
EP0573606A4 (de) 1994-12-14
US5849579A (en) 1998-12-15
DE69228055D1 (de) 1999-02-11
JPH06505636A (ja) 1994-06-30
JP2950519B2 (ja) 1999-09-20
EP0573606A1 (de) 1993-12-15
EP0573606B1 (de) 1998-12-30
ATE175242T1 (de) 1999-01-15
WO1992015700A1 (en) 1992-09-17

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Representative=s name: VONNEMANN, KLOIBER & KOLLEGEN, 80796 MUENCHEN