DE69221969T2 - Polymerlösung mit niedriger Viskosität für die Kapillar-Elektrophorese - Google Patents

Polymerlösung mit niedriger Viskosität für die Kapillar-Elektrophorese

Info

Publication number
DE69221969T2
DE69221969T2 DE69221969T DE69221969T DE69221969T2 DE 69221969 T2 DE69221969 T2 DE 69221969T2 DE 69221969 T DE69221969 T DE 69221969T DE 69221969 T DE69221969 T DE 69221969T DE 69221969 T2 DE69221969 T2 DE 69221969T2
Authority
DE
Germany
Prior art keywords
polymer solution
low viscosity
capillary electrophoresis
viscosity polymer
electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE69221969T
Other languages
English (en)
Other versions
DE69221969D1 (de
Inventor
Paul David Grossman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24940896&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69221969(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of DE69221969D1 publication Critical patent/DE69221969D1/de
Application granted granted Critical
Publication of DE69221969T2 publication Critical patent/DE69221969T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
DE69221969T 1991-07-17 1992-07-15 Polymerlösung mit niedriger Viskosität für die Kapillar-Elektrophorese Revoked DE69221969T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/731,771 US5126021A (en) 1991-07-17 1991-07-17 Low-viscosity polymer solution for capillary electrophoresis

Publications (2)

Publication Number Publication Date
DE69221969D1 DE69221969D1 (de) 1997-10-09
DE69221969T2 true DE69221969T2 (de) 1998-02-12

Family

ID=24940896

Family Applications (2)

Application Number Title Priority Date Filing Date
DE199292306490T Pending DE523982T1 (de) 1991-07-17 1992-07-15 Polymerloesung mit niedriger viskositaet fuer die kapillar-elektrophorese.
DE69221969T Revoked DE69221969T2 (de) 1991-07-17 1992-07-15 Polymerlösung mit niedriger Viskosität für die Kapillar-Elektrophorese

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE199292306490T Pending DE523982T1 (de) 1991-07-17 1992-07-15 Polymerloesung mit niedriger viskositaet fuer die kapillar-elektrophorese.

Country Status (4)

Country Link
US (1) US5126021A (de)
EP (1) EP0523982B1 (de)
JP (1) JP2976387B2 (de)
DE (2) DE523982T1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374527A (en) * 1993-01-21 1994-12-20 Applied Biosystems, Inc. High resolution DNA sequencing method using low viscosity medium
US5891313A (en) 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
WO1995016910A1 (en) * 1993-12-17 1995-06-22 Perkin-Elmer Corporation Uncharged polymers for separation of biomolecules by capillary electrophoresis
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5534123A (en) * 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
AU1375097A (en) * 1995-12-28 1997-07-28 Novartis Ag Capillary electrophoretic separation method using a fillable polymer solution as separating agent
JP2000511633A (ja) 1996-05-24 2000-09-05 ノバルティス アクチエンゲゼルシャフト キャピラリーアフィニティーゲル電気泳動を用いた物質混合物の分離法
US5989399A (en) * 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US6001232A (en) * 1996-09-04 1999-12-14 The Research Foundation Of State University Of New York Separation medium for capillary electrophoresis
US5749824A (en) * 1996-09-05 1998-05-12 Ranpak Corp. Conversion machine loader and method
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6117911A (en) * 1997-04-11 2000-09-12 Neorx Corporation Compounds and therapies for the prevention of vascular and non-vascular pathologies
US5958694A (en) * 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
JP4515640B2 (ja) 1999-02-02 2010-08-04 カリパー・ライフ・サイエンシズ・インク． タンパク質特性決定方法、装置及びシステム
EP1208126B1 (de) 1999-07-02 2006-04-12 Symyx Technologies, Inc. Polymerverzweigungen zur immobilisierung von molekülen auf oberflächen oder substraten, wobei die polymere wasserlösliche oder wasserdispergierbare segmente und sonden aufweisen
US6716948B1 (en) 1999-07-31 2004-04-06 Symyx Technologies, Inc. Controlled-architecture polymers and use thereof as separation media
US6770698B1 (en) 2000-06-05 2004-08-03 The Research Foundation At State University Of New York Polymer solution for separation of charged macromolecules by electrophoresis
DE60106053T2 (de) * 2000-07-21 2005-11-17 Aclara BioSciences, Inc., Mountain View Verfahren und vorrichtungen zur kapillarelektrophorese mit einer norbornenhaltigen oberflächenbeschichtung
US6706162B1 (en) 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
US7205161B2 (en) * 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
US6797139B2 (en) 2001-03-19 2004-09-28 Applera Corporation Detection cell for guiding excitation light therein and method for using same
US20030085127A1 (en) * 2001-06-13 2003-05-08 Symyx Technologies, Inc. Polymer additive useful in separation media
EP1406720B1 (de) * 2001-07-19 2013-12-04 Life Technologies Corporation Puffer für elektrophorese und verwendung davon
WO2003073233A2 (en) * 2002-02-26 2003-09-04 Pharmacia Corporation Sequence detection system calculator
JP2005534749A (ja) 2002-07-29 2005-11-17 アプレラ コーポレイション グラフト共重合体、それらの調製、およびキャピラリー電気泳動での使用
US7108775B2 (en) * 2002-11-08 2006-09-19 Applera Corporation Apparatus and method for confining eluted samples in electrophoresis systems
US20050025741A1 (en) 2003-05-15 2005-02-03 Lau Aldrich N.K. Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
US20070272556A1 (en) * 2003-05-22 2007-11-29 Kane Thomas E Electrophoresis Capillaries Having Reduced Amounts of -Oh
US9442091B2 (en) 2011-01-31 2016-09-13 Qiagen Mansfield, Inc. Reduced artifact denaturing capillary electrophoresis of nucleic acids
JP5821358B2 (ja) * 2011-07-20 2015-11-24 ソニー株式会社 核酸抽出方法及び核酸抽出用カートリッジ

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865707A (en) * 1986-10-21 1989-09-12 Northeastern University Capillary gel electrophoresis columns
US5096554A (en) * 1989-08-07 1992-03-17 Applied Biosystems, Inc. Nucleic acid fractionation by counter-migration capillary electrophoresis
US5164055A (en) * 1990-01-29 1992-11-17 Applied Biosystems, Inc. High-viscosity polymer matrix and methods
DE69007773T2 (de) * 1990-02-13 1994-09-22 Bio Rad Laboratories Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren.

Also Published As

Publication number Publication date
DE69221969D1 (de) 1997-10-09
DE523982T1 (de) 1993-07-08
JPH05215717A (ja) 1993-08-24
EP0523982B1 (de) 1997-09-03
EP0523982A1 (de) 1993-01-20
JP2976387B2 (ja) 1999-11-10
US5126021A (en) 1992-06-30

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Legal Events

Date Code Title Description
8363 Opposition against the patent
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8331 Complete revocation