DE523982T1 - Polymerloesung mit niedriger viskositaet fuer die kapillar-elektrophorese. - Google Patents
Polymerloesung mit niedriger viskositaet fuer die kapillar-elektrophorese.Info
- Publication number
- DE523982T1 DE523982T1 DE199292306490T DE92306490T DE523982T1 DE 523982 T1 DE523982 T1 DE 523982T1 DE 199292306490 T DE199292306490 T DE 199292306490T DE 92306490 T DE92306490 T DE 92306490T DE 523982 T1 DE523982 T1 DE 523982T1
- Authority
- DE
- Germany
- Prior art keywords
- polymer solution
- low viscosity
- capillary electrophoresis
- electrophoresis
- capillary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/731,771 US5126021A (en) | 1991-07-17 | 1991-07-17 | Low-viscosity polymer solution for capillary electrophoresis |
Publications (1)
Publication Number | Publication Date |
---|---|
DE523982T1 true DE523982T1 (de) | 1993-07-08 |
Family
ID=24940896
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE199292306490T Pending DE523982T1 (de) | 1991-07-17 | 1992-07-15 | Polymerloesung mit niedriger viskositaet fuer die kapillar-elektrophorese. |
DE69221969T Revoked DE69221969T2 (de) | 1991-07-17 | 1992-07-15 | Polymerlösung mit niedriger Viskosität für die Kapillar-Elektrophorese |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69221969T Revoked DE69221969T2 (de) | 1991-07-17 | 1992-07-15 | Polymerlösung mit niedriger Viskosität für die Kapillar-Elektrophorese |
Country Status (4)
Country | Link |
---|---|
US (1) | US5126021A (de) |
EP (1) | EP0523982B1 (de) |
JP (1) | JP2976387B2 (de) |
DE (2) | DE523982T1 (de) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5374527A (en) * | 1993-01-21 | 1994-12-20 | Applied Biosystems, Inc. | High resolution DNA sequencing method using low viscosity medium |
US5891313A (en) * | 1993-11-23 | 1999-04-06 | The Perkin-Elmer Corp. | Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks |
US6358385B1 (en) * | 1993-12-17 | 2002-03-19 | The Perkin-Elmer Corporation | Polymers for separation of biomolecules by capillary electrophoresis |
AU1254295A (en) * | 1993-12-17 | 1995-07-03 | Perkin-Elmer Corporation, The | Uncharged polymers for separation of biomolecules by capillary electrophoresis |
US5423966A (en) * | 1994-01-25 | 1995-06-13 | Perkin-Elmer Corporation | On line ion contaminant removal apparatus and method for capillary electrophoresis |
US5582705A (en) * | 1995-05-19 | 1996-12-10 | Iowa State University Research Foundation, Inc. | Multiplexed capillary electrophoresis system |
US5534123A (en) * | 1995-07-10 | 1996-07-09 | Molecular Dynamics | Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis |
AU1375097A (en) * | 1995-12-28 | 1997-07-28 | Novartis Ag | Capillary electrophoretic separation method using a fillable polymer solution as separating agent |
WO1997045721A1 (en) * | 1996-05-24 | 1997-12-04 | Novartis Ag | Process for separating mixtures of substances using capillary affinity gel electrophoresis |
US6001232A (en) * | 1996-09-04 | 1999-12-14 | The Research Foundation Of State University Of New York | Separation medium for capillary electrophoresis |
US5989399A (en) * | 1996-09-04 | 1999-11-23 | The Research Foundation Of State University Of New York | Effective surface treatment for a new separation medium in electrophoresis |
US5749824A (en) * | 1996-09-05 | 1998-05-12 | Ranpak Corp. | Conversion machine loader and method |
US6235471B1 (en) | 1997-04-04 | 2001-05-22 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
US6391622B1 (en) | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
AU6959898A (en) * | 1997-04-11 | 1998-11-11 | David J. Grainger | Compounds and therapies for the prevention of vascular and non-vascular pathol ogies |
US5958694A (en) | 1997-10-16 | 1999-09-28 | Caliper Technologies Corp. | Apparatus and methods for sequencing nucleic acids in microfluidic systems |
US5948227A (en) | 1997-12-17 | 1999-09-07 | Caliper Technologies Corp. | Methods and systems for performing electrophoretic molecular separations |
AU758309B2 (en) * | 1999-02-02 | 2003-03-20 | Caliper Life Sciences, Inc. | Methods, devices and systems for characterizing proteins |
EP1208126B1 (de) | 1999-07-02 | 2006-04-12 | Symyx Technologies, Inc. | Polymerverzweigungen zur immobilisierung von molekülen auf oberflächen oder substraten, wobei die polymere wasserlösliche oder wasserdispergierbare segmente und sonden aufweisen |
US6716948B1 (en) | 1999-07-31 | 2004-04-06 | Symyx Technologies, Inc. | Controlled-architecture polymers and use thereof as separation media |
US6770698B1 (en) | 2000-06-05 | 2004-08-03 | The Research Foundation At State University Of New York | Polymer solution for separation of charged macromolecules by electrophoresis |
JP4896349B2 (ja) * | 2000-07-21 | 2012-03-14 | アクララ バイオサイエンシーズ, インコーポレイテッド | ノルボルネンベースの表面コーティングを用いるキャピラリー電気泳動のための方法およびデバイス。 |
US6706162B1 (en) | 2000-09-25 | 2004-03-16 | Applera Corporation | High speed, high resolution compositions, methods, and kits for capillary electrophoresis |
AU2002246978A1 (en) * | 2001-01-10 | 2002-07-24 | Symyx Technologies, Inc. | Polymer brushes for immobilizing molecules to a surface |
US6797139B2 (en) | 2001-03-19 | 2004-09-28 | Applera Corporation | Detection cell for guiding excitation light therein and method for using same |
WO2002101375A2 (en) * | 2001-06-13 | 2002-12-19 | Symyx Technologies, Inc. | Polymer additive for use in separation media |
EP1406720B1 (de) * | 2001-07-19 | 2013-12-04 | Life Technologies Corporation | Puffer für elektrophorese und verwendung davon |
WO2003073233A2 (en) * | 2002-02-26 | 2003-09-04 | Pharmacia Corporation | Sequence detection system calculator |
EP2275463A1 (de) * | 2002-07-29 | 2011-01-19 | Life Technologies Corporation | Pfropfcopolymere, ihre Herstellung und Verwendung in der Kapillarelektrophorese |
US7108775B2 (en) * | 2002-11-08 | 2006-09-19 | Applera Corporation | Apparatus and method for confining eluted samples in electrophoresis systems |
US20050025741A1 (en) * | 2003-05-15 | 2005-02-03 | Lau Aldrich N.K. | Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis |
WO2004106888A2 (en) * | 2003-05-22 | 2004-12-09 | Spectrumedix Llc | Electrophoresis capillaries having reduced amounts of -oh |
US9442091B2 (en) | 2011-01-31 | 2016-09-13 | Qiagen Mansfield, Inc. | Reduced artifact denaturing capillary electrophoresis of nucleic acids |
JP5821358B2 (ja) * | 2011-07-20 | 2015-11-24 | ソニー株式会社 | 核酸抽出方法及び核酸抽出用カートリッジ |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4865707A (en) * | 1986-10-21 | 1989-09-12 | Northeastern University | Capillary gel electrophoresis columns |
US5096554A (en) * | 1989-08-07 | 1992-03-17 | Applied Biosystems, Inc. | Nucleic acid fractionation by counter-migration capillary electrophoresis |
US5164055A (en) * | 1990-01-29 | 1992-11-17 | Applied Biosystems, Inc. | High-viscosity polymer matrix and methods |
EP0442177B1 (de) * | 1990-02-13 | 1994-03-30 | Bio-Rad Laboratories, Inc. | Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren |
-
1991
- 1991-07-17 US US07/731,771 patent/US5126021A/en not_active Expired - Lifetime
-
1992
- 1992-07-07 JP JP4201783A patent/JP2976387B2/ja not_active Expired - Fee Related
- 1992-07-15 EP EP92306490A patent/EP0523982B1/de not_active Revoked
- 1992-07-15 DE DE199292306490T patent/DE523982T1/de active Pending
- 1992-07-15 DE DE69221969T patent/DE69221969T2/de not_active Revoked
Also Published As
Publication number | Publication date |
---|---|
DE69221969D1 (de) | 1997-10-09 |
EP0523982B1 (de) | 1997-09-03 |
US5126021A (en) | 1992-06-30 |
DE69221969T2 (de) | 1998-02-12 |
JPH05215717A (ja) | 1993-08-24 |
EP0523982A1 (de) | 1993-01-20 |
JP2976387B2 (ja) | 1999-11-10 |
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