DE69022165D1 - Klonierungs-Plasmid-Vektor, Primer-Vektor davon und Herstellungsverfahren einer cDNA-Bank. - Google Patents

Klonierungs-Plasmid-Vektor, Primer-Vektor davon und Herstellungsverfahren einer cDNA-Bank.

Info

Publication number
DE69022165D1
DE69022165D1 DE69022165T DE69022165T DE69022165D1 DE 69022165 D1 DE69022165 D1 DE 69022165D1 DE 69022165 T DE69022165 T DE 69022165T DE 69022165 T DE69022165 T DE 69022165T DE 69022165 D1 DE69022165 D1 DE 69022165D1
Authority
DE
Germany
Prior art keywords
vector
production method
cdna library
primer
library production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69022165T
Other languages
English (en)
Other versions
DE69022165T2 (de
Inventor
Seishi Kato
Takashi Aoki
Yuri Umezawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sagami Chemical Research Institute
Original Assignee
Sagami Chemical Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP23833290A external-priority patent/JP2994010B2/ja
Application filed by Sagami Chemical Research Institute filed Critical Sagami Chemical Research Institute
Publication of DE69022165D1 publication Critical patent/DE69022165D1/de
Application granted granted Critical
Publication of DE69022165T2 publication Critical patent/DE69022165T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/525—Tumour necrosis factor [TNF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE1990622165 1989-10-31 1990-10-31 Klonierungs-Plasmid-Vektor, Primer-Vektor davon und Herstellungsverfahren einer cDNA-Bank. Expired - Fee Related DE69022165T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP28367489 1989-10-31
JP23833290A JP2994010B2 (ja) 1989-10-31 1990-09-07 クローニングベクタープラスミド、それから誘導されるベクタープライマー及びそれを用いたｃＤＮＡバンクの作製方法

Publications (2)

Publication Number Publication Date
DE69022165D1 true DE69022165D1 (de) 1995-10-12
DE69022165T2 DE69022165T2 (de) 1996-04-04

Family

ID=26533645

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1990622165 Expired - Fee Related DE69022165T2 (de) 1989-10-31 1990-10-31 Klonierungs-Plasmid-Vektor, Primer-Vektor davon und Herstellungsverfahren einer cDNA-Bank.

Country Status (2)

Country Link
EP (1) EP0426455B1 (de)
DE (1) DE69022165T2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019809A1 (en) * 1990-06-18 1991-12-26 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Eukaryotic expression vector system
US5932441A (en) * 1995-03-31 1999-08-03 Amersham International Plc Vectors for differential expression
EP0910653A2 (de) * 1996-04-26 1999-04-28 University Of Pittsburgh Cytoplasmatisches genexpressionssystem welches eine prokaryotisches autogene rnapolymerase verwendet
CN1264744A (zh) * 1999-02-26 2000-08-30 上海生元基因开发有限公司 一种通过循环差减进行大规模cDNA克隆和测序的方法
KR100453279B1 (ko) * 2000-04-27 2004-10-15 주식회사 서린바이오사이언스 양쪽 3'끝이 동일하거나 비상보적인 하나의 염기돌출부위를 갖는 벡터, ＥｃｌＨＫＩ 카세트 및 상기벡터와 ＥｃｌＨＫＩ 카세트의 이용방법
FR2824844B1 (fr) * 2001-05-18 2003-09-19 Genoway Procede de clonage par double selection et vecteurs pour ce procede

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4843003A (en) * 1984-02-17 1989-06-27 Fred Hutchinson Cancer Research Center Process for producing shortened target DNA fragments usable in sequencing large DNA segments

Also Published As

Publication number Publication date
EP0426455A2 (de) 1991-05-08
DE69022165T2 (de) 1996-04-04
EP0426455B1 (de) 1995-09-06
EP0426455A3 (en) 1991-09-11

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee