DE68915231D1 - Verfahren und Vorrichtung zur multiplen Elektrophorese mit kontrollierter Wanderung von Makromolekülen in Gelplatten. - Google Patents

Verfahren und Vorrichtung zur multiplen Elektrophorese mit kontrollierter Wanderung von Makromolekülen in Gelplatten.

Info

Publication number
DE68915231D1
DE68915231D1 DE68915231T DE68915231T DE68915231D1 DE 68915231 D1 DE68915231 D1 DE 68915231D1 DE 68915231 T DE68915231 T DE 68915231T DE 68915231 T DE68915231 T DE 68915231T DE 68915231 D1 DE68915231 D1 DE 68915231D1
Authority
DE
Germany
Prior art keywords
macromolecules
gel plates
controlled migration
multiple electrophoresis
electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68915231T
Other languages
English (en)
Other versions
DE68915231T2 (de
Inventor
Bernard Dutertre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bertin Technologies SAS
Original Assignee
Bertin et Cie SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR8811626A external-priority patent/FR2636860B1/fr
Application filed by Bertin et Cie SA filed Critical Bertin et Cie SA
Application granted granted Critical
Publication of DE68915231D1 publication Critical patent/DE68915231D1/de
Publication of DE68915231T2 publication Critical patent/DE68915231T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE68915231T 1988-09-06 1989-09-04 Verfahren und Vorrichtung zur multiplen Elektrophorese mit kontrollierter Wanderung von Makromolekülen in Gelplatten. Expired - Fee Related DE68915231T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8811626A FR2636860B1 (fr) 1988-09-06 1988-09-06 Procede et dispositif d'electrophorese multiple pour assurer la migration controlee de macromolecules dans des plaques rectangulaires de gel
FR888815246A FR2639253B2 (fr) 1988-09-06 1988-11-23 Dispositif d'electrophorese multiple pour assurer la migration controlee de macromolecules dans des plaques rectangulaires de gel

Publications (2)

Publication Number Publication Date
DE68915231D1 true DE68915231D1 (de) 1994-06-16
DE68915231T2 DE68915231T2 (de) 1994-12-01

Family

ID=26226863

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68915231T Expired - Fee Related DE68915231T2 (de) 1988-09-06 1989-09-04 Verfahren und Vorrichtung zur multiplen Elektrophorese mit kontrollierter Wanderung von Makromolekülen in Gelplatten.

Country Status (10)

Country Link
US (1) US5102524A (de)
EP (1) EP0358556B1 (de)
JP (1) JPH03502136A (de)
KR (1) KR900701376A (de)
AU (1) AU624180B2 (de)
BR (1) BR8907077A (de)
CA (1) CA1332157C (de)
DE (1) DE68915231T2 (de)
FR (1) FR2639253B2 (de)
WO (1) WO1990002601A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9000444D0 (en) * 1990-01-09 1990-03-07 Hybaid Ltd Analysis of biological molecule samples
GB9024428D0 (en) * 1990-11-09 1991-01-02 Elchrom Ltd Apparatus and method for submerged gel electrophoresis
FR2670129B1 (fr) * 1990-12-10 1993-04-02 Bertin & Cie Procede de preparation d'une membrane garnie d'une couche d'un gel et cadre de support d'une telle membrane concu pour la mise en óoeuvre de ce procede.
US5234559A (en) * 1991-12-31 1993-08-10 E. I. Du Pont De Nemours And Company Apparatus for direct blotting and automated electrophoresis, transfer and detection and processes utilizing the apparatus thereof
FR2699423B1 (fr) * 1992-12-22 1995-03-17 Bertin & Cie Procédé et dispositif d'électrophorèse multiple pour la migration et le transfert de macromolécules.
US5449446A (en) * 1994-03-09 1995-09-12 Verma; Sumeet Multi-purpose electrophoresis apparatus
JPH11508042A (ja) * 1995-06-08 1999-07-13 ビジブル ジェネティクス インコーポレイテッド バイオポリマーの分析のためのナノ規模で造られた分離マトリックス、それを製造する方法および使用する方法
DE19544127C1 (de) * 1995-11-27 1997-03-20 Gimsa Jan Dr Verfahren und Vorrichtung zur Erzeugung von Resonanzerscheinungen in Partikelsuspensionen und ihre Verwendung
US6689267B2 (en) 2002-03-11 2004-02-10 Center For Blood Research Multi-plate electrophoresis system having non-mechanical buffer circulation
US7150813B2 (en) * 2003-06-12 2006-12-19 Palo Alto Research Center Incorporated Isoelectric focusing (IEF) of proteins with sequential and oppositely directed traveling waves in gel electrophoresis
US7156970B2 (en) * 2003-06-12 2007-01-02 Palo Alto Research Center Incorporated Distributed multi-segmented reconfigurable traveling wave grids for separation of proteins in gel electrophoresis
US8715476B2 (en) * 2009-12-11 2014-05-06 Bio-Rad Laboratories, Inc. Instrument for independent electrotransfer in multiple cassettes
EP2986359A4 (de) 2014-03-11 2017-02-15 Bio-rad Laboratories, Inc. Dip-stick-western-blot
AU2016353162B2 (en) 2015-11-10 2023-02-23 Woodham Biotechnology Holdings, LLC Gel electrophoresis and transfer combination using conductive polymers and method of use
US9702851B1 (en) 2016-06-17 2017-07-11 Woodham Biotechnology Holdings, LLC Gel electrophoresis and transfer combination using conductive polymers and method of use

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2107092A1 (en) * 1971-02-15 1972-08-31 Max Planck Gesellschaft Two-dimensional gel-electrophoresis plant - sepn of dissolved particles esp of proteins
DE2204697A1 (de) * 1972-02-01 1973-08-09 Steffen Guenther Dr M Gussmann Hochspannungs-gel-elektrophoresegeraet
US3879280A (en) * 1974-04-16 1975-04-22 Us Health Gel slab electrophoresis cell and electrophoresis apparatus utilizing same
US4088561A (en) * 1977-06-27 1978-05-09 The United States Of America As Represented By The United States Department Of Energy Apparatus for electrophoresis separation
US4416761A (en) * 1982-07-27 1983-11-22 The United States Of America As Represented By The Department Of Health And Human Services Multi slab gel casting electrophoresis apparatus
US4473452A (en) * 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
FR2568485B1 (fr) * 1984-08-06 1990-03-23 Rhone Poulenc Rech Appareil de fractionnement par electrophorese de solutions contenant des proteines, utilisable notamment pour le fractionnement du plasma humain
GB8523801D0 (en) * 1985-09-26 1985-10-30 Jones K W Vacuum molecular transfer(blotting)apparatus
US4740283A (en) * 1986-02-27 1988-04-26 University Patents, Inc. Pulsed-field gradient gel electrophoretic apparatus
EP0256737B1 (de) * 1986-08-13 1993-03-24 The Board Of Trustees Of The Leland Stanford Junior University Elektrophorese, welche homogene oder inhomogene elektrische Felder mit bestimmtem Umkreis benutzt
US4994166A (en) * 1989-08-22 1991-02-19 Bio-Rad Laboratories, Inc. Single apparatus for slab gel electrophoresis and blotting

Also Published As

Publication number Publication date
JPH03502136A (ja) 1991-05-16
EP0358556B1 (de) 1994-05-11
DE68915231T2 (de) 1994-12-01
AU624180B2 (en) 1992-06-04
KR900701376A (ko) 1990-12-01
EP0358556A1 (de) 1990-03-14
CA1332157C (fr) 1994-09-27
FR2639253B2 (fr) 1991-07-26
WO1990002601A1 (fr) 1990-03-22
AU4217289A (en) 1990-04-02
BR8907077A (pt) 1991-01-08
US5102524A (en) 1992-04-07
FR2639253A2 (fr) 1990-05-25

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee