DE665383C - Counter for blood corpuscles and other small particles in a carrier fluid - Google Patents
Counter for blood corpuscles and other small particles in a carrier fluidInfo
- Publication number
- DE665383C DE665383C DES117406D DES0117406D DE665383C DE 665383 C DE665383 C DE 665383C DE S117406 D DES117406 D DE S117406D DE S0117406 D DES0117406 D DE S0117406D DE 665383 C DE665383 C DE 665383C
- Authority
- DE
- Germany
- Prior art keywords
- counting
- small particles
- counter
- carrier fluid
- network
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002245 particle Substances 0.000 title claims description 5
- 210000000601 blood cell Anatomy 0.000 title description 7
- 239000012530 fluid Substances 0.000 title 1
- 239000007788 liquid Substances 0.000 claims description 5
- 238000005530 etching Methods 0.000 claims description 3
- 238000006748 scratching Methods 0.000 claims description 3
- 230000002393 scratching effect Effects 0.000 claims description 3
- 238000004820 blood count Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 description 7
- 239000006059 cover glass Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150358—Strips for collecting blood, e.g. absorbent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Ecology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Optics & Photonics (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
Zählvorrichtung für Blutkörper und andere in einer Trägerflüssigkeit befindliche kleine Teilchen Die Erfindung betrifft Zählvorrichtungen für Blutkörper und andere in einer Trägerflüssigkeit befindliche kleine Teilchen, bei welchen das Zählnetz auf einer durchsichtigen Platte angebracht ist.Counting device for blood cells and others in a carrier liquid Small Particles Located This invention relates to blood cell counting devices and other small particles in a carrier liquid in which the Counting net is attached to a transparent plate.
Es sind bereits Zählvorrichtungen für Blutkörper und andere in einer Trägerflüssigkeit befindliche kleine Teilchen bekanntgeworden, bei welchen das Zählnetz in eine zur Aufnahme der Trägerflüssigkeit dienende durchsichtige Glasplatte eingeritzt ist.There are already counters for blood cells and others in one Small particles located in the carrier liquid become known, in which the counting network scratched into a transparent glass plate used to hold the carrier liquid is.
Diese älteren Zählplatten sind zum Zählen von Blutkörpern o. dgl. jedoch insofern ungünstig, als sich die Blutkörper auf der mit Rillen versehenen Glasfläche nicht gleichmäßig über die ganze Zählfläche verteilen. Außerdem sind die Zähllinien der bekannten Zählplatten bei erträglicher Tiefe nur schlecht wahrnehmbar, da sie hinsichtlich ihrer Lichtdurchlässigkeit gegenüber den Zählflächen keine oder nur sehr geringe Unterschiede aufweisen und daher von diesen Flächen auch nur schwer zu unterscheiden sind. Nur wenn die Rillen sehr tief in die Glasoberfläche eingeritzt werden, können sie einigermaßen sichtbar gemacht werden. Solche verhältnismäßig tiefen Zähllinien sind jedoch, wie oben bereits gesagt, nachteilig, da sich die Blutkörper in ihnen anzusammeln suchen.These older counting plates are for counting blood cells or the like. however, it is disadvantageous in that the blood cells are located on the grooved Do not distribute the glass surface evenly over the entire counting surface. Also are the counting lines of the known counting plates are difficult to perceive at a tolerable depth, because they have no or in terms of their transparency to the counting surfaces show only very small differences and therefore only difficult from these areas are to be distinguished. Only if the grooves are carved very deeply into the glass surface they can be made more or less visible. Such proportionally However, as already mentioned above, deep counting lines are disadvantageous because the Seek to accumulate blood cells in them.
Demgegenüber ist auf der Zählplatte nach der Erfindung eine dünne, metallische, lichtdurchlässige, das Zählnetz bildende Schicht vorgesehen, wobei das Zählnetz unter Vermeidung eines Ritzens oder Ätzens der Platte selbst durch Entfernen von Teilen der Deckschicht erzeugt werden kann.In contrast, a thin, metallic, translucent layer forming the counting network is provided, wherein the counting network while avoiding scratching or etching the plate itself Removal of parts of the top layer can be generated.
Im Falle einer solchen dünnen, metallischen, lichtdurchlässigen Deckschicht auf der Glasplatte sind die durch Entfernen von Teilen der Deckschicht gebildeten Zähllinien oder Zählflächen gut sichtbar, da sich ihre Lichtdurchlässigkeit von derjenigen der Zählflächen bzw. der Zähllinien deutlich unterscheidet. Außerdem besteht bei dem erfindungsgemäß ausgebildeten Zählnetz weniger Anlaß, daß sich die Blutkörper in den Zähllinien ansammeln, als es bei einem durch Ritzen oder Ätzen der Glasplatte selbst hergestellten Zählnetz der Fall ist. Denn die Furchen in der Metallschicht sind weniger tief als die in die Glasplatte selbst geritzten Rillen und trotzdem als Zählnetz erkennbar.In the case of such a thin, metallic, translucent cover layer on the glass plate are those formed by removing parts of the cover layer Counting lines or counting areas are clearly visible, as their light transmission is different from that of the counting areas or the counting lines are clearly different. aside from that there is less reason for the counting network designed according to the invention that the Blood cells accumulate in the counting lines than it would in one by scratching or etching the counting network made by the glass plate itself is the case. Because the furrows in the Metal layers are less deep than the grooves carved into the glass plate itself and still recognizable as a counting network.
Auf der Zeichnung ist ein Ausführungsbeispiel des Gegenstandes der Erfindung dargestellt.In the drawing is an embodiment of the subject matter of Invention shown.
Fig. i zeigt eine Aufsicht.Fig. I shows a plan view.
Fig. ? ist eine Seitenansicht mit aufgelegtem Deckglas; zwischen dem Deckglas und der Zählplatte befindet sich die zu untersuchende Lösung.Fig.? is a side view with the cover glass in place; between the The solution to be examined is located on the cover slip and the counting plate.
Fig.3 zeigt in stark vergrößertem Maßstabe einen Teil der Oberfläche der Zählplatte.3 shows a part of the surface on a greatly enlarged scale the counting plate.
Fig..I ist ein Schnitt durch die Zählplatte nach der Linie .I-.I der Fig. 3.Fig..I is a section through the counting plate along the line .I-.I of the Fig. 3.
Die gezeichnete Zählvorrichtung besteht aus einer Glasplatte io, die auf ihrer einen Seite im Abstand voneinanderliegende erhöhte Rippen i i trägt. Die Rippen i i dienen als Auflager für das übliche Deckglas Zwischen den Rippen i i liegt ein breiter erhabener Ansatz 1?, dessen Höhe etwas geringer ist als diejenige der Rippen i i. Die. Oberfläche des erhabenen Ansatzes 12 ist ge°: schliffen oder poliert. Im Anschluß an diese Fertigbehandlung wird in bekannter Weise die dünne, metallische, lichtdurchlässige Schicht durch Kathodenzerstäubung o. dgl. aufgebracht und das Zählnetz durch Entfernen von Teilen der Deckschicht ausgebildet. Bei dem dargestellten Ausführungsbeispiel ist der Ansatz 12 durch eine Nut 13 in zwei Abschnitte aufgeteilt, von denen jeder ein aufgeteiltes Feld (Zählnetz 24) aufweist. Das Vorhandensein der -Nut 13 ist selbstverständlich für die Erfindung nicht wesentlich; es kann natürlich jede beliebige Anzahl von aufg #tel ilten Feldern 12 vorgesehen sein.The drawn counting device consists of a glass plate io, the on their one side at a distance from each other increased Ribs i i wears. The ribs i i serve as supports for the usual cover glass between the ribs i i have a broad, raised attachment 1?, the height of which is somewhat lower is than that of the ribs i i. The. Surface of the raised projection 12 is ge °: ground or polished. Following this final treatment is known in Remove the thin, metallic, translucent layer by cathodic sputtering o. The like. Applied and the counting network by removing parts of the top layer educated. In the illustrated embodiment, the approach 12 is through a Groove 13 divided into two sections, each of which has a divided field (counting network 24). The presence of the groove 13 is a matter of course for the invention not essential; Of course, any number of fields can be split up 12 may be provided.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DES117406D DE665383C (en) | 1935-03-07 | 1935-03-07 | Counter for blood corpuscles and other small particles in a carrier fluid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DES117406D DE665383C (en) | 1935-03-07 | 1935-03-07 | Counter for blood corpuscles and other small particles in a carrier fluid |
Publications (1)
Publication Number | Publication Date |
---|---|
DE665383C true DE665383C (en) | 1938-09-23 |
Family
ID=7534227
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DES117406D Expired DE665383C (en) | 1935-03-07 | 1935-03-07 | Counter for blood corpuscles and other small particles in a carrier fluid |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE665383C (en) |
-
1935
- 1935-03-07 DE DES117406D patent/DE665383C/en not_active Expired
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