DE6604278U - DIAGNOSTIC DEVICE - Google Patents

Description

Utility model registration in Germany M 53 o44 / 3o h Gbm Anra: S.E. Massgill Company

"Diagnostic device"

The innovation relates to diagnostic devices and in particular swabs or swab-like devices which are directly brought into contact with the focus of the disease which is believed to contain disease-causing microorganisms. The innovation further relates to the direct production of cultures of micro-organisms to prove for the purpose of their presence or absence _o More specifically, the innovation relates to a design of the device, which while allowing the detection above, but not when it it is more necessary that the smear is applied to a tube or plate that contains a suitable medium for the smear.

As is well known, the procedure for this verification is such that the doctor identifies the presumed infection area in the microorganisms may be present, for example touching the throat, nose or the like of a patient with a swab. This is called usually: "take a smear" »

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Postal check account Nuremberg 882 60 - Bayerische Vereinsbank Nuremberg 20 58553

This smear is then put into a specially prepared tube or applied to a plate on which a nutrient medium is arranged, which causes the microorganisms to develop further Then the tube or plate is subjected to the incubation conditions exposed, whereupon the doctor can determine whether illness = generating microorganisms are present or not »

The primary purpose of the innovation is to create a device to create, which makes this known process for the doctor much easier because the transfer of the smear is made not applicable.

Another purpose of the innovation is to create a device that enables the doctor to make direct contact with the focus of the disease Having a nutrient medium that allows incubation and the determination of whether pathogenic micro-organisms are present are available, can be done directly with this nutrient medium

Another purpose of the innovation is to create specific nutrient media that are solidified to such an extent that; them in a certain Can be brought into shape so that contact is possible directly at the source of the disease.

Another aim of the innovation is to create specific breeding grounds which, in addition to deformation, also have the Have the property that they encourage the growth of microorganisms prevent men other than the species to be detected, so that the growth of a micro-organism in this nutrient medium is a definite Indicates the presence of this specific microorganism forms

With regard to the advantages and features of the innovation presented above and other advantages and features, this mainly exists in that a test device for the detection of the presence or absence of micro-organisms in a given Area of a human or animal patient is created, which consists essentially of a sterilized ^ container and

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a device that supports the culture medium, which is detachably arranged in the container, so that the device with the nutrient medium after it has been removed from the container the focus of the disease in which micro-organisms are suspected, can be brought into contact, after which the device is placed back in the container for the purpose of incubation *

IiIe Neuer »un | c also relates to a device for Preparation of smears from foci of disease suspected of having microorganisms and a method of detection the presence or absence of micro-organisms in the area · This device is essentially the best * from an elongated carrier and from a device for receiving nutrient media, which are arranged at the end of the carrier and brought into contact with the focus of the disease so that they are thereafter exposed to the incubation conditions without having to transfer the smear to a separately arranged culture medium or container is required, which contains a special nutrient medium.

The innovation is described below with the aid of exemplary embodiments explained in more detail with the aid of schematic drawings

Show it:

Figure 1 shows a container with a cap for receiving the swab device under sterile conditions,

Figure 2 shows a swab according to the innovation

Figure 3 shows another embodiment of the swab according to the innovation o

In FIG. 1, a tube Io is shown which has a thread 11 has on which a cap 12 can be screwed to close the tube. This makes it possible to use the tube after it and its contents have been sterilized, to be kept under aseptic conditions. "

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In Figure 2, an elongated rod 15 is shown, ■ j

which can be made of wood or plastic, for example (

can and has a thickening of cotton wool or the like 14 at one end, on which the nutrient medium 15 is arranged. "This swab device is sterilized and inserted into the tube Io,
which is also sterilized. The cap 12 remains screwed on,
until the device is used. If it is needed, it will
the cap removed, the swab pulled out of the tube | and the end of the device provided with the base plate with the focus of the disease, for example the throat, the ear, | ^y

brought into contact with the nose or the like, whereupon jj "

put the swab back into the tube and the contents of the | Incubation conditions, for example, a temperature of
25 to 57 ° C. The presence of micro-organisms is then determined at the end of the incubation period.

In Figure ^ a rod 16 is shown, which is preferably made of plastic in this case and a side
Has extension I7 «. It is arranged so that a total of one
T-shape is created. The extension can also be in the form of a
Arrowhead or a similar shape. It enables the nutrient medium 18 to be formed directly on the end of the rod 16 and prevents it from sliding off the rod
Swab can be used in exactly the same way as the
Swab according to Figure 2, namely to determine the presence
produced by rubbing the swab directly on the surface of the tissue to be examined ©

The innovation also suggests the normal
Altering media for two reasons, namely s

1. to achieve greater dimensional stability, which enables ff, d
is attached and

it is made possible that the nutrient medium is placed directly on the rod |

»· · Ο ο e · ti · β ·« a o

Ιβ β β

2, the introduction of an indicator into the culture medium, which in the presence of certain pathogenic micro-organisms creates a characteristic color, as well as the introduction of an antibiotic agent, which the growth prevented by bacteria, polygamy not interested in the detection to be carried out, and which are other than those which produce the particular color with the indicator, and the like

The composition of these nutrient media after the innovation is described below

Known nutrient media contain up to about 2 # agar as a solidifying agent. However, the innovation suggests using 5% agar for the nutrient media so that they have greater dimensional stability, so that they become "malleable" and in this way on the stick can be arranged, avoiding excessive crumbling or the like when handling and developing cultures. Even more than 3 % agar can be used. However, it has been shown that the best results are achieved with about] 5 % agar ·

With regard to the second measure proposed above, it is known that, for example, Candida Albicans together with Wisrauth result in a very characteristic dark brown or black color as an indicator · Furthermore, the innovation suggests that to assign neocmyin to the culture medium in order to prevent the growth of bacteria, but not to the growth of Candida albicans affect · ο If therefore after touching the swab with the tissue to be examined and, after the incubation period, the characteristic dark brown or black color on the Swab emerges, this is positive evidence of its presence of Candida Albicans. It should be noted that neomycin does not prevent the growth of other forms of bacteria, such as Penicillin · These other forms also do not produce the characteristic coloration that the presence of Candida Albicans would have Show·

Examples of the composition of nutrient media are given below. However, they are not intended to restrict the scope of protection of the innovation.
example 1

A diagnostic medium according to the innovation is described below, which is used to detect the presence of Candida Albicans in vaginal infections. This Έ sis medium is set antibafcwriell by the addition of neomycin sulfate. The composition is as follows

Beef extract oil Glycol 1.0 Dextrose 1.0 Bismuth sulfite as indicator 0.8 MeomycinsuUCat 0, Agar 3.0 Water q _o s. loo

The nutrient medium is molded onto a heart stick with a cotton ball at one end.The swab created in this way is then inserted into the sterile tube and only removed again when it is used, whereby the swab is rubbed directly on the surface of the tissue to be examined The swab is then put back into the tube and subjected to the incubation conditions at 25 to yj ° C. When Candida Albicans is present, black colored colonies will look barely while other fungal organisms will not grow. The growth of bacteria is hindered by both the neomycin sulfate and the bismuth sulfite indicator

This replaces the well-known method of using a cotton ball. or a metallic spatula, the smear is removed and applied to an agar surface, which is arranged in a tube or on a plate. Example 2

Culture medium for the detection of alpha and beta hemolytic streptococci and staphylococci.
Beef heart fusion 500 g
Tryptosis Io g

Sodium Chloride 5g A fi Π Λ9 7 ft

Agar Jog ^ VUl4i »O

Water q.s., looo ml. (= Milliliter)

example

Culture medium for the detection of bacterial growth in the urine: ^w Io g 2.5 g EBM agar 5 g lo, o g Peptone 5 g 3o, o g Lactose 2 g 2nd Floor Sucrose 3o g lo, o g Dipotassiuia Phosphates ο g 75 g Agar 0.065 g : 5.0 g Eosin Y 1,000 ml 30.0 g Methylene blue metallic glow with E. Coli 1,000 ml Water g.s. It shows a green one Detection of staphylococci Ho Media Example 4 Yeast extract Tryptosis gelatin Lactose 1 D-mannitol Sodium chloride Dipotassium phosphate Agar Water q «s _o Example 5

Culture medium for the detection of Proteurs urea agar basis

Peptone 1 * 9 "g

Dextrose 1.9 g

Sodium chloride 5, og

Monopotassium phosphate 2, ο g

Urea 2o, o g

red phenol o, ol2. s

Agar 3o β

Water q.s. 1 000 ml

In all of the above examples, after the media has been sterilized, the nutrient medium is applied to a sterile cotton swab. as in Figure 2, or on a plastic rod which is provided with a lateral extension, as in Figure 3 · Then the Kährboden is under introduced into the previously sterilized glass or plastic tube according to FIG. 1 under aseptic conditions

The sterility of certain specimens is checked and determined whether it has been impaired by the manufacturing process.

Claims (1)

Nuremberg, September 15th, 1969 Bu.-Ki. Re: Utility model registration in Germany M 53 o44 / 3o h Gbm "Diagnostic device" Note S. E. Massengill Company SCHJTZ CLAIMS Ie experimental device for determining the presence of microorganisms in human or animal tissues, characterized in that they consist essentially of a sterile Container and a device for receiving nutrient media, which is detachably arranged in the container, so that they are made of removed from it and brought into contact with the tissue to be examined, whereupon it is used for the purpose of incubation is returned to the container 2 «Device according to claim 1, characterized in that the The device carrying the culture medium consists of its own handle or dergle, at one end of which there is a culture medium in solid State is arranged 3> o experimental device according to claim 1, characterized in that that the device carrying the nutrient medium consists of a wooden handle with a cotton ball at one end and on the breeding ground is arranged for him » 4ο experimental device according to claim 1, characterized in that that the device carrying the nutrient medium consists of an elongated handle, on which at one end a lateral port set is arranged, which is surrounded by the nutrient medium in the solid state 5. Experimental device according to claim 4, characterized in that that the handle is made of plastic ■ ··· * ft · ft * a · · · • · i «j« # ■ · * · A · ·. t IBt it * β 9 O »* * t β 6. Apparatus for producing cultures derived from human or animal tissues, thereby characterized in that the gate direction consists of an elongated carrier on which a device for receiving at one end of culture media is arranged ^ which with the tissue in Is brought into contact, whereupon the medium is exposed to the incubation conditions without the need to to carry out a transfer to a culture medium separate from the device. 7 · Device according to claim 6, characterized in a sterile container in which the device is arranged as long as it is not in contact with the tissue. 8. Device for making smears from human or animal tissues for determining the presence of disease-causing micro-organisms, characterized in that that it consists of an elongated carrier which at one end has at least one lateral extension on which a Culture medium is arranged in the solid state. 9 · Device according to claim δ, characterized in that the lateral extension is T-shaped * Ioο device according to claim 8, characterized in that the lateral extension is arrow-shaped. 11. Device according to claims 8 to Iq, characterized in that that the device is placed in a sterile container * 12 »Device according to claim 7, characterized in that the? Culture medium contains at least 5 percent by weight agar, so that it can both be connected to the elongated support and is also dimensionally stable. If 13 «breeding ground for the cultivation of micro-organisms, thereby
marked, derr he in addition to increasing the nutrients
its dimensional stability, also about J weight percent
Contains agar. 14e nutrient medium according to claim IJ, characterized in that it contains an indicator that works together with special microorganisms produced a special color ■ 15o nutrient medium according to claim IJ, characterized in that it contains an antibiotic agent which determines the growth Inhibits micro-organisms while it does not affect the growth of other micro-organisms. 16 »Culture medium according to claim IJ, characterized in that it contains neomycin, which the growth of certain other
Inhibits micro-organisms. 17 · Nutrient medium according to claim IJ, characterized in that it; / contains an indicator which, together with certain microorganisms, produces a characteristic color and that
the nutrient medium also contains an antibiotic agent which inhibits the growth of certain micro-organisms, but does not affect other certain micro-organisms.