DE60227394D1 - Fernfeld lichtmikroskopische methode und vorrichtung zur bestimmung von mindestens einem objekt kleiner als die benutzte wellenlänge - Google Patents

Fernfeld lichtmikroskopische methode und vorrichtung zur bestimmung von mindestens einem objekt kleiner als die benutzte wellenlänge

Info

Publication number
DE60227394D1
DE60227394D1 DE60227394T DE60227394T DE60227394D1 DE 60227394 D1 DE60227394 D1 DE 60227394D1 DE 60227394 T DE60227394 T DE 60227394T DE 60227394 T DE60227394 T DE 60227394T DE 60227394 D1 DE60227394 D1 DE 60227394D1
Authority
DE
Germany
Prior art keywords
labelled
elements
labelling
determining
field
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60227394T
Other languages
English (en)
Inventor
Christoph Cremer
Antonio Virgilio Failla
Benno Albrecht
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaet Heidelberg
Original Assignee
Universitaet Heidelberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet Heidelberg filed Critical Universitaet Heidelberg
Application granted granted Critical
Publication of DE60227394D1 publication Critical patent/DE60227394D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/66—Trinkets, e.g. shirt buttons or jewellery items
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Theoretical Computer Science (AREA)
  • Multimedia (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Length Measuring Devices By Optical Means (AREA)
DE60227394T 2001-10-09 2002-10-09 Fernfeld lichtmikroskopische methode und vorrichtung zur bestimmung von mindestens einem objekt kleiner als die benutzte wellenlänge Expired - Lifetime DE60227394D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32802101P 2001-10-09 2001-10-09
PCT/EP2002/011343 WO2003031951A1 (en) 2001-10-09 2002-10-09 Far yield light microscopical method, system and computer program product for analysing at least one object having a subwavelength size

Publications (1)

Publication Number Publication Date
DE60227394D1 true DE60227394D1 (de) 2008-08-14

Family

ID=23279165

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60227394T Expired - Lifetime DE60227394D1 (de) 2001-10-09 2002-10-09 Fernfeld lichtmikroskopische methode und vorrichtung zur bestimmung von mindestens einem objekt kleiner als die benutzte wellenlänge

Country Status (5)

Country Link
US (1) US7298461B2 (de)
EP (2) EP1436597B1 (de)
AT (2) ATE399990T1 (de)
DE (1) DE60227394D1 (de)
WO (1) WO2003031951A1 (de)

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FR2846445B1 (fr) * 2002-10-29 2005-04-08 Claude Lambert Procede d'authentification par marquage ou tracage chimique d'un objet ou d'une substance.
US20100089804A1 (en) * 2002-10-29 2010-04-15 Claude Lambert Method for identifying a substance or object using a plurality of excitation vectors
DE102004039035A1 (de) 2004-04-07 2005-10-27 EuroPhoton GmbH Gesellschaft für optische Sensorik Verfahren und Vorrichtung zur Fluoreszenz-Lebensdauer-Imaging-Nanoskopie
WO2006044660A2 (en) * 2004-10-14 2006-04-27 Vanderbilt University Functionalized solid lipid nanoparticles and methods of making and using same
WO2007094758A2 (en) * 2005-02-09 2007-08-23 S3I, Llc Method and system for detecting, classifying and identifying particles
EP2453239B1 (de) 2005-05-23 2017-04-26 Harald F. Hess Optische Mikroskopie mit transformierbaren optischen Markern
DE102006062823B4 (de) * 2006-03-01 2010-11-25 Leica Microsystems Cms Gmbh Verfahren und Mikroskop zur räumlich hochauflösenden Untersuchung von Proben
WO2007129325A2 (en) * 2006-05-09 2007-11-15 Xceed Imaging Ltd. Optical sub wavelength super resolution imaging system and method
US8217992B2 (en) * 2007-01-11 2012-07-10 The Jackson Laboratory Microscopic imaging techniques
US8821799B2 (en) * 2007-01-26 2014-09-02 Palo Alto Research Center Incorporated Method and system implementing spatially modulated excitation or emission for particle characterization with enhanced sensitivity
DE102007033737A1 (de) * 2007-07-18 2009-01-22 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zum Bestimmen eines Messwerts auf der Basis von Einzelmolekülereignissen
US8947516B2 (en) * 2007-09-26 2015-02-03 Massachusetts Institute Of Technology High-resolution 3D imaging of single semiconductor nanocrystals
FR2922658B1 (fr) * 2007-10-18 2011-02-04 Centre Nat Rech Scient Systeme d'illuminations structuree d'un echantillon
WO2009115108A1 (en) * 2008-03-19 2009-09-24 Ruprecht-Karls-Universität Heidelberg A method and an apparatus for localization of single dye molecules in the fluorescent microscopy
US7772569B2 (en) 2008-04-01 2010-08-10 The Jackson Laboratory 3D biplane microscopy
DE102010007676A1 (de) * 2010-02-10 2011-08-11 Ohnesorge, Frank, Dr., 91054 Konzept für lateral aufgelöste Fourier Transformations Infrarot Spektroskopie unterhalb/jenseits des Beugungslimits - Anwendungen für optisches (aber auch elektronisches) schnelles Auslesen von ultrakleinen Speicherzellen in Form von lumineszierenden Quantentrögen - sowie in der Biologie/Kristallographie
WO2011112634A2 (en) 2010-03-08 2011-09-15 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
WO2012142111A1 (en) * 2011-04-12 2012-10-18 Tripath Imaging, Inc. Method for preparing quantitative video-microscopy and associated system
US9213106B2 (en) * 2011-06-08 2015-12-15 Battelle Memorial Institute Method of measuring luminescence of a material
US20140073520A1 (en) * 2011-12-23 2014-03-13 California Institute Of Technology Imaging chromosome structures by super-resolution fish with single-dye labeled oligonucleotides
DE102012216002B3 (de) * 2012-05-18 2013-09-12 Leica Microsystems Cms Gmbh Schaltung und Verfahren zum Erzeugen von periodischen Steuersignalen sowie Mikroskop und Verfahren zum Steuern eines Mikroskops
EP2757402B1 (de) * 2013-01-22 2016-03-30 FEI Company Verfahren zum Betrachten von Proben mit einem Fluoreszenzmikroskop
CN105453231B (zh) * 2013-08-08 2019-06-11 应用材料公司 用于使用耗尽光束形成亚微米特征结构的反应物的光子活化
DE102013019348A1 (de) * 2013-08-15 2015-02-19 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie
DE102013019347A1 (de) * 2013-08-15 2015-02-19 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie
US9921161B1 (en) 2015-01-08 2018-03-20 Daniel Feldkhun Structured light active localization microscopy
DE102015105018A1 (de) 2015-03-31 2016-10-06 Laser-Laboratorium Göttingen e.V. Verfahren und Rasterfluoreszenzlichtmikroskop zum mehrdimensional hochauflösenden Abbilden einer Struktur oder eines Wegs eines Partikels in einer Probe
DE102017107915A1 (de) * 2016-07-18 2018-01-18 Carl Zeiss Meditec Ag System zur Augentherapie mittels Gewebebearbeitung durch nichtlineare Wechselwirkung
EP3538941A4 (de) 2016-11-10 2020-06-17 The Trustees of Columbia University in the City of New York Schnelles hochauflösendes bildgebungsverfahren für grosse proben
DE102017111578A1 (de) 2017-05-29 2018-11-29 Wladimir Schaufler Verfahren zur Unterscheidung einzelner fluoreszierender Markierungsstoffmoleküle in der SPDM-Lokalisationsmikroskopie durch deren zeitliches Langzeit-Emissionsverhalten über 10ms

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AU9251898A (en) * 1997-07-10 1999-02-08 Ruprecht-Karls-Universitat Heidelberg Wave field microscope, method for a wave field microscope, including for dna sequencing, and calibration method for wave field microscopy
DE10052583B4 (de) 2000-10-23 2011-07-14 Ruprecht-Karls-Universität Heidelberg, 69120 Verfahren zur Optimierung der molekularen Multiplex-Diagnostik von Microtissue Arrays mit Hilfe von Virtual Cell Nucleus Imageing
DE10052823B4 (de) 2000-10-24 2014-11-20 Ruprecht-Karls-Universität Heidelberg Verfahren zur lichtmikroskopischen Analyse der Topologie molekularer Strukturen in Rasteranordnungen

Also Published As

Publication number Publication date
WO2003031951A1 (en) 2003-04-17
US7298461B2 (en) 2007-11-20
EP1436597B1 (de) 2008-07-02
ATE539345T1 (de) 2012-01-15
US20050059681A1 (en) 2005-03-17
EP1436597A1 (de) 2004-07-14
EP1983330B1 (de) 2011-12-28
EP1983330A1 (de) 2008-10-22
ATE399990T1 (de) 2008-07-15

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