DE602006012786D1 - System und Methode zur Konzentrierung und Lyse von Zellen oder Viren - Google Patents
System und Methode zur Konzentrierung und Lyse von Zellen oder VirenInfo
- Publication number
- DE602006012786D1 DE602006012786D1 DE602006012786T DE602006012786T DE602006012786D1 DE 602006012786 D1 DE602006012786 D1 DE 602006012786D1 DE 602006012786 T DE602006012786 T DE 602006012786T DE 602006012786 T DE602006012786 T DE 602006012786T DE 602006012786 D1 DE602006012786 D1 DE 602006012786D1
- Authority
- DE
- Germany
- Prior art keywords
- lysis
- viruses
- cells
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0424—Dielectrophoretic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050123161A KR101157175B1 (ko) | 2005-12-14 | 2005-12-14 | 세포 또는 바이러스의 농축 및 용해용 미세유동장치 및방법 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602006012786D1 true DE602006012786D1 (de) | 2010-04-22 |
Family
ID=37876849
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602006012786T Active DE602006012786D1 (de) | 2005-12-14 | 2006-12-14 | System und Methode zur Konzentrierung und Lyse von Zellen oder Viren |
Country Status (4)
Country | Link |
---|---|
US (2) | US7959862B2 (de) |
EP (1) | EP1797956B1 (de) |
KR (1) | KR101157175B1 (de) |
DE (1) | DE602006012786D1 (de) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001040454A1 (en) | 1999-11-30 | 2001-06-07 | Oncosis | Method and apparatus for selectively targeting specific cells within a cell population |
WO2002037938A2 (en) | 2000-10-24 | 2002-05-16 | Oncosis Llc | Method and device for selectively targeting cells within a three -dimensional specimen |
WO2008104916A2 (en) * | 2007-02-27 | 2008-09-04 | Koninklijke Philips Electronics N.V. | A cell lysis and/or mixing device |
KR100988945B1 (ko) * | 2008-04-17 | 2010-10-20 | 재단법인서울대학교산학협력재단 | 마이크로 채널을 이용한 혈관 내 특정부위에 세포를유도·고정하기 위한 시뮬레이션 장치 및 이를 이용하는시뮬레이션 방법 |
US8435465B2 (en) * | 2008-11-03 | 2013-05-07 | Cfd Research Corporation | Microfluidic biological extraction chip |
KR20110106436A (ko) * | 2009-01-09 | 2011-09-28 | 신텔렉트 인코포레이티드 | 세포의 유전적 분석 |
US8788213B2 (en) | 2009-01-12 | 2014-07-22 | Intrexon Corporation | Laser mediated sectioning and transfer of cell colonies |
AT508394B1 (de) * | 2009-07-06 | 2011-12-15 | Mayer Robert | Gerät und verfahren zur anwendung der durch magnetische feldvariation modulierten ringstrominduktion in elektrisch leitfähigen nano-partikeln zur mechanischen einwirkung auf zelluläre membranen |
GB0914762D0 (en) * | 2009-08-24 | 2009-09-30 | Univ Glasgow | Fluidics apparatus and fluidics substrate |
WO2011071912A1 (en) * | 2009-12-07 | 2011-06-16 | Yale University | Label-free cellular manipulation and sorting via biocompatible ferrofluids |
AU2011320908A1 (en) | 2010-10-28 | 2013-05-23 | Yale University | Microfluidic processing of target species in ferrofluids |
KR101776215B1 (ko) | 2010-10-29 | 2017-09-08 | 삼성전자 주식회사 | 세포 파쇄용 마이크로 디바이스 및 이를 이용한 세포 파쇄 방법 |
US8895297B2 (en) | 2010-10-29 | 2014-11-25 | Samsung Electronics Co., Ltd. | Micro-device and methods for disrupting cells |
US8986986B2 (en) | 2010-10-29 | 2015-03-24 | Samsung Electronics Co., Ltd. | Cell lysis device and methods of lysing cells or viruses |
DE102011007035A1 (de) * | 2011-04-08 | 2012-10-11 | Robert Bosch Gmbh | Verfahren, LOC und Analysevorrichtung zur Lyse von Zellen und PCR-Amplifikation |
US9580679B2 (en) | 2012-09-21 | 2017-02-28 | California Institute Of Technology | Methods and devices for sample lysis |
WO2014144782A2 (en) | 2013-03-15 | 2014-09-18 | Ancera, Inc. | Systems and methods for active particle separation |
US20160299132A1 (en) | 2013-03-15 | 2016-10-13 | Ancera, Inc. | Systems and methods for bead-based assays in ferrofluids |
WO2015048009A1 (en) * | 2013-09-27 | 2015-04-02 | Arizona Board Of Regents On Behale Of Arizona State University | System and method for laser lysis |
US11285490B2 (en) | 2015-06-26 | 2022-03-29 | Ancera, Llc | Background defocusing and clearing in ferrofluid-based capture assays |
KR101741104B1 (ko) | 2015-09-01 | 2017-05-30 | 한국과학기술연구원 | 대용량 수인성 병원체 현장 포집 및 농축시스템 |
KR101726954B1 (ko) | 2015-09-30 | 2017-04-27 | 이화여자대학교 산학협력단 | 피에조 결합체를 포함하는 휴대용 세포파쇄장치 |
CN107090403B (zh) * | 2017-03-22 | 2019-06-18 | 清华大学 | 一种细胞裂解系统和方法 |
EP3666881A1 (de) * | 2018-12-12 | 2020-06-17 | AIT Austrian Institute of Technology GmbH | Gekoppelte sortierung und elektrische behandlung biologischer zellen |
CN115397967A (zh) | 2020-04-28 | 2022-11-25 | 美国西门子医学诊断股份有限公司 | 声电泳裂解设备和方法 |
WO2022241248A1 (en) * | 2021-05-13 | 2022-11-17 | Fluid-Screen, Inc. | Techniques for releasing cellular contents from a microorganism using a microfluidic system |
WO2022271194A1 (en) * | 2021-06-25 | 2022-12-29 | Arrington Abron | Pathogen destruction system and method using magnetic markers |
WO2023059894A1 (en) * | 2021-10-08 | 2023-04-13 | Baebies, Inc. | Digital microfluidics multi-dynamic range parallel biochemical assays |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9301122D0 (en) * | 1993-01-21 | 1993-03-10 | Scient Generics Ltd | Method of analysis/separation |
US6071394A (en) * | 1996-09-06 | 2000-06-06 | Nanogen, Inc. | Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis |
US6641708B1 (en) * | 1996-01-31 | 2003-11-04 | Board Of Regents, The University Of Texas System | Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation |
US6221673B1 (en) * | 1997-11-25 | 2001-04-24 | Microsensor Systems Inc. | Materials, method and apparatus for detection and monitoring of chemical species |
US6352838B1 (en) * | 1999-04-07 | 2002-03-05 | The Regents Of The Universtiy Of California | Microfluidic DNA sample preparation method and device |
US6824664B1 (en) * | 1999-11-04 | 2004-11-30 | Princeton University | Electrode-less dielectrophorises for polarizable particles |
ES2288154T3 (es) * | 2000-04-13 | 2008-01-01 | Wako Pure Chemical Industries, Ltd | Construccion de electrodos para aparato dielectroforetico y separacion por dielectroforesis. |
US6790330B2 (en) * | 2000-06-14 | 2004-09-14 | Board Of Regents, The University Of Texas System | Systems and methods for cell subpopulation analysis |
CA2417341A1 (en) * | 2000-08-08 | 2002-02-14 | Jing Cheng | Methods for manipulating moieties in microfluidic systems |
CN1325909C (zh) * | 2000-09-27 | 2007-07-11 | 清华大学 | 用于微粒操纵与微粒导向的装置及其使用方法 |
US6761811B2 (en) * | 2001-03-27 | 2004-07-13 | The Regents Of The University Of California | Multi-stage separations based on dielectrophoresis |
US6730204B2 (en) * | 2001-03-27 | 2004-05-04 | The Regents Of The University Of California | Three dimensional separation trap based on dielectrophoresis and use thereof |
US7419574B2 (en) * | 2001-06-20 | 2008-09-02 | Cummings Eric B | Dielectrophoresis device and method having non-uniform arrays for manipulating particles |
US7014747B2 (en) * | 2001-06-20 | 2006-03-21 | Sandia Corporation | Dielectrophoretic systems without embedded electrodes |
JP2003000226A (ja) | 2001-06-25 | 2003-01-07 | Ryokusei Mes Kk | 細胞破砕装置 |
CA2462914A1 (en) * | 2001-10-11 | 2003-04-17 | Aviva Biosciences Corporation | Methods, compositions, and automated systems for separating rare cells from fluid samples |
FR2831084B1 (fr) * | 2001-10-22 | 2004-08-27 | Centre Nat Rech Scient | Procede et systeme pour manipuler par dielectrophorese des particules dielectriques, en particulier des cellules biologiques |
US6815209B2 (en) * | 2001-11-16 | 2004-11-09 | Cornell Research Foundation, Inc. | Laser-induced cell lysis system |
US20040011650A1 (en) * | 2002-07-22 | 2004-01-22 | Frederic Zenhausern | Method and apparatus for manipulating polarizable analytes via dielectrophoresis |
US20040018611A1 (en) * | 2002-07-23 | 2004-01-29 | Ward Michael Dennis | Microfluidic devices for high gradient magnetic separation |
US20050221341A1 (en) * | 2003-10-22 | 2005-10-06 | Shimkets Richard A | Sequence-based karyotyping |
WO2005099419A2 (en) | 2004-04-13 | 2005-10-27 | President And Fellows Of Harvard College | Manipulation and/or detection of biological samples or other objects |
KR100695160B1 (ko) | 2004-10-19 | 2007-03-14 | 삼성전자주식회사 | 레이저 및 마이크로 자성 비드를 이용한 세포 또는바이러스의 신속한 파괴 방법 및 장치 |
KR100601972B1 (ko) * | 2004-11-03 | 2006-07-18 | 삼성전자주식회사 | 레이저와 비드를 이용한 상 분리에 의한 핵산의 정제 장치및 방법 |
KR100561873B1 (ko) | 2004-11-30 | 2006-03-17 | 삼성전자주식회사 | 자유 라디칼을 이용한 세포 용해 방법 |
-
2005
- 2005-12-14 KR KR1020050123161A patent/KR101157175B1/ko not_active IP Right Cessation
-
2006
- 2006-12-14 US US11/610,938 patent/US7959862B2/en not_active Expired - Fee Related
- 2006-12-14 EP EP06126171A patent/EP1797956B1/de not_active Expired - Fee Related
- 2006-12-14 DE DE602006012786T patent/DE602006012786D1/de active Active
-
2011
- 2011-02-25 US US13/034,857 patent/US8252569B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US20070134809A1 (en) | 2007-06-14 |
EP1797956A1 (de) | 2007-06-20 |
US7959862B2 (en) | 2011-06-14 |
KR20070063189A (ko) | 2007-06-19 |
EP1797956B1 (de) | 2010-03-10 |
KR101157175B1 (ko) | 2012-07-03 |
US20110143414A1 (en) | 2011-06-16 |
US8252569B2 (en) | 2012-08-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE602006012786D1 (de) | System und Methode zur Konzentrierung und Lyse von Zellen oder Viren | |
DE602006005278D1 (de) | Vorrichtung und Methode zur Trennung und zur Rückgewinnung von kurzen Stangen | |
DE112005000800A5 (de) | Ausdampfverfahren zur Reinigung und/oder Aufkonzentrierung verunreinigter Flüssigkeiten | |
ATE507242T1 (de) | Wirtszellenspezifische bindungsmoleküle, die dazu in der lage sind, viren zu neutralisieren, und anwendungen davon | |
ATE541211T1 (de) | Verfahren zur beurteilung von zellen und zellkulturen | |
FI20051248A0 (fi) | Biologisten komponenttien rikastusyksikkö ja rikastusmenetelmä | |
GB0514909D0 (en) | Methods of nucleic acid amplification and sequencing | |
BRPI0819819A2 (pt) | Preparações, método e kits ùteis para o tratamento de tosse | |
DE602008001924D1 (de) | Verfahren und System zur Erkennung unerwünschter Inferenzen von Dokumenten | |
SG11201500371VA (en) | Methods of detecting dna, rna and protein in biological samples | |
IL192883A (en) | Methods for detecting and identifying biological beads and molecules | |
BRPI0613893A2 (pt) | sistema e método para tratar pele | |
EP1910389A4 (de) | Auftrennung und aufreinigung von nukleinsäure aus paraffinhaltigen proben | |
BR112012002140A2 (pt) | métodos para purificar recombinante a tipo desintegrina e metalopeptidase com proteína trombospondina e para inativar contaminantes de vírus em uma amostra de proteína,e, composição. | |
DE602005017924D1 (de) | Zellgewebe-mikrochip und verfahren zur bildung von zellgewebe | |
DE602006004968D1 (de) | Verfahren und anlage zur umwandlung von chromhaltigem gefährlichem abfall in ungefählriche abfälle | |
BRPI0913284A2 (pt) | determinação de perfis de concentração de efluentes e vidas úteis de cartuchos para respirador purificador de ar | |
DE602007006107D1 (de) | Verfahren zum Konzentrieren und Aufschluss von Zellen oder Viren | |
ATE540755T1 (de) | Verwendung von katalysatorzusammensetzungen und verfahren zur oxychlorierung | |
ATE512215T1 (de) | Verfahren und mittel zur anreicherung, entfernung und zum nachweis von listerien | |
DE112005003750A5 (de) | Verwendung von Moosen und/oder Flechten, Element und Verfahren zur Reduzierung des Feinstaubgehalts in der Luft | |
ATE542804T1 (de) | 5-phenyl-pentansäurederivate als matrixmetalloproteinase-inhibitoren zur behandlung von asthma und anderen erkrankungen | |
EP1863348A4 (de) | Reagens zur zellpermeabilisierung und stabilisierung sowie verwendungsverfahren dafür | |
ATE414277T1 (de) | Verfahren zur verhinderung der zeitabhängigen rna-expression in biologischen zellen | |
BRPI0921727A2 (pt) | processo e sistema para o tratamento de biomassa |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |