DE602004020473D1 - Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmitteln - Google Patents
Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmittelnInfo
- Publication number
- DE602004020473D1 DE602004020473D1 DE602004020473T DE602004020473T DE602004020473D1 DE 602004020473 D1 DE602004020473 D1 DE 602004020473D1 DE 602004020473 T DE602004020473 T DE 602004020473T DE 602004020473 T DE602004020473 T DE 602004020473T DE 602004020473 D1 DE602004020473 D1 DE 602004020473D1
- Authority
- DE
- Germany
- Prior art keywords
- lps
- protein
- alkanediols
- complexes
- separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002158 endotoxin Substances 0.000 title abstract 11
- 229920006008 lipopolysaccharide Polymers 0.000 title abstract 10
- 238000000926 separation method Methods 0.000 title abstract 3
- 239000002904 solvent Substances 0.000 title 1
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 241000894006 Bacteria Species 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 150000001298 alcohols Chemical class 0.000 abstract 1
- 150000001335 aliphatic alkanes Chemical group 0.000 abstract 1
- 238000005349 anion exchange Methods 0.000 abstract 1
- 150000001450 anions Chemical class 0.000 abstract 1
- 238000005341 cation exchange Methods 0.000 abstract 1
- 239000012501 chromatography medium Substances 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Peptides Or Proteins (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US48089903P | 2003-06-24 | 2003-06-24 | |
| US48859603P | 2003-07-18 | 2003-07-18 | |
| PCT/US2004/018992 WO2005003152A1 (en) | 2003-06-24 | 2004-06-15 | Removal of lipopolysacchrarides from protein- lipopolysaccharide complexes by nonflammable solvents |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602004020473D1 true DE602004020473D1 (de) | 2009-05-20 |
Family
ID=33567625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004020473T Expired - Lifetime DE602004020473D1 (de) | 2003-06-24 | 2004-06-15 | Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmitteln |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US20060189790A1 (de) |
| EP (1) | EP1638990B1 (de) |
| JP (1) | JP2007526225A (de) |
| AT (1) | ATE427954T1 (de) |
| CA (1) | CA2530454A1 (de) |
| DE (1) | DE602004020473D1 (de) |
| ES (1) | ES2323642T3 (de) |
| WO (1) | WO2005003152A1 (de) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6399570B1 (en) * | 1999-02-05 | 2002-06-04 | Agennix, Inc. | Antimicrobial/endotoxin neutralizing polypeptide |
| EP1638990B1 (de) * | 2003-06-24 | 2009-04-08 | N.V. Organon | Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmitteln |
| US20110159130A1 (en) | 2007-02-28 | 2011-06-30 | Sanjay Jain | Reduction of endotoxin in polysialic acids |
| ES2518290T3 (es) * | 2007-07-10 | 2014-11-04 | Glanbia Nutritionals (Ireland) Limited | Método de eliminación de endotoxinas de proteínas |
| AU2014201943B2 (en) * | 2007-07-10 | 2016-07-28 | Glanbia Nutritionals | Method for removing endotoxin from proteins |
| DK2393834T3 (da) * | 2009-02-06 | 2013-06-24 | Novozymes Biopharma Dk As | Fremgangsmåde til oprensning |
| AU2010239795B2 (en) | 2009-04-24 | 2014-07-10 | Westland Co-Operative Dairy Company Limited | Method of preparing low-iron lactoferrin |
| CN103880947B (zh) * | 2012-12-21 | 2016-01-13 | 武汉禾元生物科技股份有限公司 | 一种分离纯化高纯度重组人血清白蛋白的层析方法 |
| JP6438975B2 (ja) | 2014-06-12 | 2018-12-19 | ヒグロス インベスト ゲーエムベーハー | 溶液中の内毒素の脱マスキングの方法 |
| EP2955518B2 (de) | 2014-06-12 | 2022-01-26 | bioMérieux Deutschland GmbH | Demaskierung von Endotoxinen in Lösung |
| MX2017003927A (es) | 2014-09-24 | 2017-06-30 | Basf Se | Proceso para producir diesteres de acido tereftalico. |
| CA2962164A1 (en) | 2014-09-24 | 2016-03-31 | Basf Se | Method for producing diesters of terephthalic acid with enrichment of recirculated alcohol |
| CN106715382A (zh) | 2014-09-24 | 2017-05-24 | 巴斯夫欧洲公司 | 通过再循环醇脱水而制备对苯二甲酸二酯的方法 |
| PL3197858T3 (pl) | 2014-09-24 | 2019-08-30 | Basf Se | Sposób wytwarzania diestrów kwasu tereftalowego z cyrkulacją mieszaniny reakcyjnej |
| CN105251049B (zh) * | 2015-09-29 | 2018-04-10 | 浙江星月生物科技股份有限公司 | 一种去除生物医用材料中细菌内毒素的离子缓冲液及应用 |
| DE102016106271B4 (de) | 2016-04-06 | 2018-03-29 | Axagarius Gmbh & Co. Kg | Aufreinigung von Nukleinsäure aus einer Nukleinsäure und Endotoxin enthaltenden Probe |
| US11065600B2 (en) | 2016-05-26 | 2021-07-20 | Cytosorbents Corporation | Use of a hemocompatible porous polymer bead sorbent for removal of endotoxemia-inducing molecules |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2961293D1 (en) * | 1978-02-17 | 1982-01-14 | Nat Res Dev | Immunogenic cell envelope preparations |
| US4832751A (en) * | 1987-07-01 | 1989-05-23 | The United States Of America As Represented By The Secretary Of Agriculture | Method for reduction of endotoxin in cotton lint or dust |
| JP2577237B2 (ja) * | 1988-02-05 | 1997-01-29 | 雪印乳業株式会社 | 樹脂担体からパイロジェンを除去する方法 |
| EP0407037B1 (de) * | 1989-06-12 | 1994-09-21 | Merck & Co. Inc. | Verfahren zur Entfernung von bakteriellen Endotoxinen aus gram-negativen Polysacchariden |
| US5045456A (en) * | 1989-06-12 | 1991-09-03 | Merck & Co., Inc. | Process for removing bacterial endotoxin from gram-negative polysaccharides |
| AU691574B2 (en) * | 1994-02-07 | 1998-05-21 | Qiagen Gmbh | Process for producing endotoxin-free or endotoxin-poor nucleic acids and/or oligonucleotides for gene therapy |
| US6265542B1 (en) * | 1997-10-24 | 2001-07-24 | Genentech, Inc. | Purification of molecules |
| WO1999063076A1 (en) * | 1998-06-01 | 1999-12-09 | The Immune Response Corporation | Novel method of large scale plasmid purification |
| DE10010342A1 (de) * | 2000-03-06 | 2001-09-20 | Merck Patent Gmbh | Verfahren zur Abreicherung von Endotoxinen |
| US6966992B2 (en) * | 2001-03-19 | 2005-11-22 | Akzo Nobel Nv | Method of purification of molecules using unbranched terminal alkyldiols |
| EP1638990B1 (de) * | 2003-06-24 | 2009-04-08 | N.V. Organon | Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmitteln |
-
2004
- 2004-06-15 EP EP04755271A patent/EP1638990B1/de not_active Expired - Lifetime
- 2004-06-15 AT AT04755271T patent/ATE427954T1/de not_active IP Right Cessation
- 2004-06-15 CA CA002530454A patent/CA2530454A1/en not_active Abandoned
- 2004-06-15 ES ES04755271T patent/ES2323642T3/es not_active Expired - Lifetime
- 2004-06-15 WO PCT/US2004/018992 patent/WO2005003152A1/en active Search and Examination
- 2004-06-15 DE DE602004020473T patent/DE602004020473D1/de not_active Expired - Lifetime
- 2004-06-15 US US10/561,985 patent/US20060189790A1/en not_active Abandoned
- 2004-06-15 JP JP2006517275A patent/JP2007526225A/ja active Pending
-
2010
- 2010-05-19 US US12/783,319 patent/US8399633B2/en not_active Expired - Lifetime
-
2013
- 2013-03-12 US US13/796,838 patent/US20130267691A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE427954T1 (de) | 2009-04-15 |
| CA2530454A1 (en) | 2005-01-13 |
| US20130267691A1 (en) | 2013-10-10 |
| JP2007526225A (ja) | 2007-09-13 |
| EP1638990A1 (de) | 2006-03-29 |
| US8399633B2 (en) | 2013-03-19 |
| WO2005003152A8 (en) | 2005-03-10 |
| WO2005003152A1 (en) | 2005-01-13 |
| US20060189790A1 (en) | 2006-08-24 |
| US20110003976A1 (en) | 2011-01-06 |
| EP1638990B1 (de) | 2009-04-08 |
| ES2323642T3 (es) | 2009-07-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| R082 | Change of representative |
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| R081 | Change of applicant/patentee |
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