DE60029325T2 - Typ ii dns polymerase aus pyrococcus abyssi - Google Patents
Typ ii dns polymerase aus pyrococcus abyssi Download PDFInfo
- Publication number
- DE60029325T2 DE60029325T2 DE60029325T DE60029325T DE60029325T2 DE 60029325 T2 DE60029325 T2 DE 60029325T2 DE 60029325 T DE60029325 T DE 60029325T DE 60029325 T DE60029325 T DE 60029325T DE 60029325 T2 DE60029325 T2 DE 60029325T2
- Authority
- DE
- Germany
- Prior art keywords
- seq
- polypeptide
- sequence
- dna
- nucleotide sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241001148023 Pyrococcus abyssi Species 0.000 title claims description 60
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 title claims description 49
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 title claims description 45
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 138
- 229920001184 polypeptide Polymers 0.000 claims abstract description 128
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 127
- 238000000034 method Methods 0.000 claims abstract description 74
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 48
- 239000013598 vector Substances 0.000 claims abstract description 43
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 33
- 230000008569 process Effects 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 113
- 125000003729 nucleotide group Chemical group 0.000 claims description 75
- 239000002773 nucleotide Substances 0.000 claims description 72
- 239000012634 fragment Substances 0.000 claims description 66
- 150000001413 amino acids Chemical class 0.000 claims description 63
- 102000004169 proteins and genes Human genes 0.000 claims description 58
- 230000000694 effects Effects 0.000 claims description 45
- 238000006243 chemical reaction Methods 0.000 claims description 37
- 150000007523 nucleic acids Chemical class 0.000 claims description 31
- 230000003321 amplification Effects 0.000 claims description 29
- 238000010367 cloning Methods 0.000 claims description 29
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 29
- 102000039446 nucleic acids Human genes 0.000 claims description 26
- 108020004707 nucleic acids Proteins 0.000 claims description 26
- 239000013604 expression vector Substances 0.000 claims description 21
- 241001465754 Metazoa Species 0.000 claims description 15
- 239000000523 sample Substances 0.000 claims description 15
- 230000000295 complement effect Effects 0.000 claims description 13
- 238000006116 polymerization reaction Methods 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 9
- 239000012472 biological sample Substances 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 239000013599 cloning vector Substances 0.000 claims 1
- 244000000010 microbial pathogen Species 0.000 claims 1
- 230000010076 replication Effects 0.000 abstract description 27
- 230000004060 metabolic process Effects 0.000 abstract description 10
- 238000006065 biodegradation reaction Methods 0.000 abstract description 5
- 239000002253 acid Substances 0.000 abstract description 3
- 150000007513 acids Chemical class 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 80
- 108091006112 ATPases Proteins 0.000 description 73
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 73
- 210000004027 cell Anatomy 0.000 description 65
- 235000018102 proteins Nutrition 0.000 description 56
- 102000004190 Enzymes Human genes 0.000 description 44
- 108090000790 Enzymes Proteins 0.000 description 44
- 229940088598 enzyme Drugs 0.000 description 44
- 238000003752 polymerase chain reaction Methods 0.000 description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 42
- 230000001419 dependent effect Effects 0.000 description 41
- 229940024606 amino acid Drugs 0.000 description 39
- 235000001014 amino acid Nutrition 0.000 description 39
- 102000003939 Membrane transport proteins Human genes 0.000 description 38
- 108090000301 Membrane transport proteins Proteins 0.000 description 38
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 35
- 102000004316 Oxidoreductases Human genes 0.000 description 29
- 108090000854 Oxidoreductases Proteins 0.000 description 29
- 238000012163 sequencing technique Methods 0.000 description 29
- 102000004594 DNA Polymerase I Human genes 0.000 description 26
- 239000000872 buffer Substances 0.000 description 26
- 239000002609 medium Substances 0.000 description 26
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 24
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 24
- 102000004157 Hydrolases Human genes 0.000 description 24
- 108090000604 Hydrolases Proteins 0.000 description 24
- 108010063113 DNA Polymerase II Proteins 0.000 description 23
- 102000010567 DNA Polymerase II Human genes 0.000 description 23
- 239000011701 zinc Substances 0.000 description 23
- 108010074122 Ferredoxins Proteins 0.000 description 22
- 108010017826 DNA Polymerase I Proteins 0.000 description 21
- 102000018780 Replication Protein A Human genes 0.000 description 21
- 108010027643 Replication Protein A Proteins 0.000 description 21
- 230000017730 intein-mediated protein splicing Effects 0.000 description 21
- 108020005199 Dehydrogenases Proteins 0.000 description 20
- 101150060505 polC gene Proteins 0.000 description 20
- 108010020056 Hydrogenase Proteins 0.000 description 19
- 238000011534 incubation Methods 0.000 description 19
- 101150005648 polB gene Proteins 0.000 description 19
- 235000002639 sodium chloride Nutrition 0.000 description 19
- 239000011780 sodium chloride Substances 0.000 description 19
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 18
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 18
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 18
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 17
- 241000588724 Escherichia coli Species 0.000 description 17
- 101000983177 Streptomyces rimosus N,N-dimethyltransferase OxyT Proteins 0.000 description 17
- 102000035195 Peptidases Human genes 0.000 description 16
- 108091005804 Peptidases Proteins 0.000 description 16
- 101150008507 dnaE gene Proteins 0.000 description 16
- 101150076840 PolI gene Proteins 0.000 description 15
- 239000004365 Protease Substances 0.000 description 15
- 241000894007 species Species 0.000 description 15
- 238000003786 synthesis reaction Methods 0.000 description 15
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 15
- 241000203069 Archaea Species 0.000 description 14
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 14
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 14
- 230000006870 function Effects 0.000 description 14
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 14
- 102000018120 Recombinases Human genes 0.000 description 13
- 108010091086 Recombinases Proteins 0.000 description 13
- 102000018779 Replication Protein C Human genes 0.000 description 13
- 108010027647 Replication Protein C Proteins 0.000 description 13
- 238000000605 extraction Methods 0.000 description 13
- 239000013612 plasmid Substances 0.000 description 13
- 101100278439 Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) pol gene Proteins 0.000 description 12
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 12
- 102000003844 DNA helicases Human genes 0.000 description 12
- 108090000133 DNA helicases Proteins 0.000 description 12
- 241000205160 Pyrococcus Species 0.000 description 12
- 108090000944 RNA Helicases Proteins 0.000 description 12
- 102000004409 RNA Helicases Human genes 0.000 description 12
- 238000012512 characterization method Methods 0.000 description 12
- 210000000349 chromosome Anatomy 0.000 description 12
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 12
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 12
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 12
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 12
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 12
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 11
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 11
- 230000008436 biogenesis Effects 0.000 description 11
- 239000012429 reaction media Substances 0.000 description 11
- 239000011541 reaction mixture Substances 0.000 description 11
- 230000008439 repair process Effects 0.000 description 11
- 235000000346 sugar Nutrition 0.000 description 11
- 230000014621 translational initiation Effects 0.000 description 11
- 108090000204 Dipeptidase 1 Proteins 0.000 description 10
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 10
- 239000001888 Peptone Substances 0.000 description 10
- 108010080698 Peptones Proteins 0.000 description 10
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 10
- 102000006635 beta-lactamase Human genes 0.000 description 10
- 229940041514 candida albicans extract Drugs 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 10
- -1 inteins Proteins 0.000 description 10
- 235000019319 peptone Nutrition 0.000 description 10
- 230000009261 transgenic effect Effects 0.000 description 10
- 239000012138 yeast extract Substances 0.000 description 10
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 9
- 108010046778 molybdenum cofactor Proteins 0.000 description 9
- 229910052759 nickel Inorganic materials 0.000 description 9
- 230000006798 recombination Effects 0.000 description 9
- 239000011734 sodium Substances 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 230000032258 transport Effects 0.000 description 9
- 102000013446 GTP Phosphohydrolases Human genes 0.000 description 8
- 108091006109 GTPases Proteins 0.000 description 8
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 8
- 239000004473 Threonine Substances 0.000 description 8
- 230000027455 binding Effects 0.000 description 8
- 238000009739 binding Methods 0.000 description 8
- 239000001177 diphosphate Substances 0.000 description 8
- 235000011180 diphosphates Nutrition 0.000 description 8
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 8
- 238000010348 incorporation Methods 0.000 description 8
- 239000011159 matrix material Substances 0.000 description 8
- 230000004048 modification Effects 0.000 description 8
- 238000012986 modification Methods 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000035939 shock Effects 0.000 description 8
- 108010071550 ATP-Dependent Proteases Proteins 0.000 description 7
- 102000007566 ATP-Dependent Proteases Human genes 0.000 description 7
- 229920000936 Agarose Polymers 0.000 description 7
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 102000018697 Membrane Proteins Human genes 0.000 description 7
- 108010052285 Membrane Proteins Proteins 0.000 description 7
- 108700026244 Open Reading Frames Proteins 0.000 description 7
- 101100010170 Pyrococcus abyssi (strain GE5 / Orsay) polC gene Proteins 0.000 description 7
- 108090000340 Transaminases Proteins 0.000 description 7
- 229940098773 bovine serum albumin Drugs 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 7
- 238000009396 hybridization Methods 0.000 description 7
- 239000012528 membrane Substances 0.000 description 7
- 239000002777 nucleoside Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 7
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 7
- 229960001327 pyridoxal phosphate Drugs 0.000 description 7
- 238000005215 recombination Methods 0.000 description 7
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 7
- 229940048086 sodium pyrophosphate Drugs 0.000 description 7
- 238000000638 solvent extraction Methods 0.000 description 7
- 238000006467 substitution reaction Methods 0.000 description 7
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 7
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 7
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 7
- 108010028984 3-isopropylmalate dehydratase Proteins 0.000 description 6
- 108090000084 Antiporters Proteins 0.000 description 6
- 102000003669 Antiporters Human genes 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 6
- 108700023372 Glycosyltransferases Proteins 0.000 description 6
- 108010072039 Histidine kinase Proteins 0.000 description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- HDAJUGGARUFROU-JSUDGWJLSA-L MoO2-molybdopterin cofactor Chemical compound O([C@H]1NC=2N=C(NC(=O)C=2N[C@H]11)N)[C@H](COP(O)(O)=O)C2=C1S[Mo](=O)(=O)S2 HDAJUGGARUFROU-JSUDGWJLSA-L 0.000 description 6
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 6
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 6
- 101100010182 Pyrococcus abyssi (strain GE5 / Orsay) polB gene Proteins 0.000 description 6
- 241000432808 Pyrococcus abyssi GE5 Species 0.000 description 6
- 102000011929 Succinate-CoA Ligases Human genes 0.000 description 6
- 108010075728 Succinate-CoA Ligases Proteins 0.000 description 6
- 102000003929 Transaminases Human genes 0.000 description 6
- 108020002494 acetyltransferase Proteins 0.000 description 6
- 102000005421 acetyltransferase Human genes 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 6
- 238000001035 drying Methods 0.000 description 6
- 239000002158 endotoxin Substances 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 229920006008 lipopolysaccharide Polymers 0.000 description 6
- 108091000115 phosphomannomutase Proteins 0.000 description 6
- 108091008146 restriction endonucleases Proteins 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 102000009067 AAA Proteins Human genes 0.000 description 5
- 108010087671 AAA Proteins Proteins 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 5
- 101000977023 Azospirillum brasilense Uncharacterized 17.8 kDa protein in nodG 5'region Proteins 0.000 description 5
- 101000961984 Bacillus thuringiensis Uncharacterized 30.3 kDa protein Proteins 0.000 description 5
- 108010078791 Carrier Proteins Proteins 0.000 description 5
- 101000644901 Drosophila melanogaster Putative 115 kDa protein in type-1 retrotransposable element R1DM Proteins 0.000 description 5
- 108010067770 Endopeptidase K Proteins 0.000 description 5
- 101000747702 Enterobacteria phage N4 Uncharacterized protein Gp2 Proteins 0.000 description 5
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 description 5
- 241000620209 Escherichia coli DH5[alpha] Species 0.000 description 5
- 101000758599 Escherichia coli Uncharacterized 14.7 kDa protein Proteins 0.000 description 5
- 241000206602 Eukaryota Species 0.000 description 5
- 101000768930 Lactococcus lactis subsp. cremoris Uncharacterized protein in pepC 5'region Proteins 0.000 description 5
- 101000976302 Leptospira interrogans Uncharacterized protein in sph 3'region Proteins 0.000 description 5
- 101000778886 Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) Uncharacterized protein LA_2151 Proteins 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 108010038807 Oligopeptides Proteins 0.000 description 5
- 102000015636 Oligopeptides Human genes 0.000 description 5
- 229930185560 Pseudouridine Natural products 0.000 description 5
- PTJWIQPHWPFNBW-UHFFFAOYSA-N Pseudouridine C Natural products OC1C(O)C(CO)OC1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-UHFFFAOYSA-N 0.000 description 5
- 108020004511 Recombinant DNA Proteins 0.000 description 5
- 101001121571 Rice tungro bacilliform virus (isolate Philippines) Protein P2 Proteins 0.000 description 5
- 101000818098 Spirochaeta aurantia Uncharacterized protein in trpE 3'region Proteins 0.000 description 5
- 101001026590 Streptomyces cinnamonensis Putative polyketide beta-ketoacyl synthase 2 Proteins 0.000 description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 5
- 101000750896 Synechococcus elongatus (strain PCC 7942 / FACHB-805) Uncharacterized protein Synpcc7942_2318 Proteins 0.000 description 5
- 239000007983 Tris buffer Substances 0.000 description 5
- 101000916321 Xenopus laevis Transposon TX1 uncharacterized 149 kDa protein Proteins 0.000 description 5
- 101000760088 Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1) 20.9 kDa protein Proteins 0.000 description 5
- 229960000643 adenine Drugs 0.000 description 5
- WGDUUQDYDIIBKT-UHFFFAOYSA-N beta-Pseudouridine Natural products OC1OC(CN2C=CC(=O)NC2=O)C(O)C1O WGDUUQDYDIIBKT-UHFFFAOYSA-N 0.000 description 5
- 230000001851 biosynthetic effect Effects 0.000 description 5
- 230000032823 cell division Effects 0.000 description 5
- 238000001962 electrophoresis Methods 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 229930027917 kanamycin Natural products 0.000 description 5
- 229960000318 kanamycin Drugs 0.000 description 5
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
- 229930182823 kanamycin A Natural products 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 235000019419 proteases Nutrition 0.000 description 5
- PTJWIQPHWPFNBW-GBNDHIKLSA-N pseudouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-GBNDHIKLSA-N 0.000 description 5
- 108091008020 response regulators Proteins 0.000 description 5
- 230000019491 signal transduction Effects 0.000 description 5
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 5
- 239000011593 sulfur Substances 0.000 description 5
- 229910052717 sulfur Inorganic materials 0.000 description 5
- 108091008023 transcriptional regulators Proteins 0.000 description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
- KPULXFNPTWGJQH-UHFFFAOYSA-N 3-hydroxy-4-oxo-4-propan-2-yloxybutanoic acid Chemical compound CC(C)OC(=O)C(O)CC(O)=O KPULXFNPTWGJQH-UHFFFAOYSA-N 0.000 description 4
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 4
- 108010009924 Aconitate hydratase Proteins 0.000 description 4
- 102000006268 Alanine-tRNA ligase Human genes 0.000 description 4
- 108010058060 Alanine-tRNA ligase Proteins 0.000 description 4
- 102000034263 Amino acid transporters Human genes 0.000 description 4
- 108050005273 Amino acid transporters Proteins 0.000 description 4
- 108020004634 Archaeal DNA Proteins 0.000 description 4
- 102000047411 CBS domains Human genes 0.000 description 4
- 108700037257 CBS domains Proteins 0.000 description 4
- 102000014914 Carrier Proteins Human genes 0.000 description 4
- 108020004705 Codon Proteins 0.000 description 4
- 102100039868 Cytoplasmic aconitate hydratase Human genes 0.000 description 4
- 101710088194 Dehydrogenase Proteins 0.000 description 4
- 108010016626 Dipeptides Proteins 0.000 description 4
- 108010089760 Electron Transport Complex I Proteins 0.000 description 4
- 102000008013 Electron Transport Complex I Human genes 0.000 description 4
- 102100031780 Endonuclease Human genes 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 4
- 241001288713 Escherichia coli MC1061 Species 0.000 description 4
- 102000003983 Flavoproteins Human genes 0.000 description 4
- 108010057573 Flavoproteins Proteins 0.000 description 4
- 108010024636 Glutathione Proteins 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 4
- 102000003960 Ligases Human genes 0.000 description 4
- 108090000364 Ligases Proteins 0.000 description 4
- 108010006519 Molecular Chaperones Proteins 0.000 description 4
- 101150008132 NDE1 gene Proteins 0.000 description 4
- YOAHKNVSNCMZGQ-XPWFQUROSA-N P(1),P(4)-bis(5'-adenosyl) tetraphosphate Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(=O)OP(O)(=O)OC[C@H]([C@@H](O)[C@H]1O)O[C@H]1N1C(N=CN=C2N)=C2N=C1 YOAHKNVSNCMZGQ-XPWFQUROSA-N 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 102000030605 Phosphomannomutase Human genes 0.000 description 4
- 108091000080 Phosphotransferase Proteins 0.000 description 4
- 108010048287 Short Chain Dehydrogenase-Reductases Proteins 0.000 description 4
- 102000009105 Short Chain Dehydrogenase-Reductases Human genes 0.000 description 4
- 241000192581 Synechocystis sp. Species 0.000 description 4
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 4
- 239000007984 Tris EDTA buffer Substances 0.000 description 4
- 102100021436 UDP-glucose 4-epimerase Human genes 0.000 description 4
- 108090000637 alpha-Amylases Proteins 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 229960003180 glutathione Drugs 0.000 description 4
- 238000011835 investigation Methods 0.000 description 4
- 229960000310 isoleucine Drugs 0.000 description 4
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 4
- 238000007834 ligase chain reaction Methods 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- 102000020233 phosphotransferase Human genes 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 108010042660 rRNA (adenosine-O-2'-)methyltransferase Proteins 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 235000019157 thiamine Nutrition 0.000 description 4
- 229960003495 thiamine Drugs 0.000 description 4
- 239000011721 thiamine Substances 0.000 description 4
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 238000012800 visualization Methods 0.000 description 4
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 3
- 108010071218 3'-phosphoadenylyl-5'-phosphosulfate reductase Proteins 0.000 description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 102100040149 Adenylyl-sulfate kinase Human genes 0.000 description 3
- 102000003924 Asparagine-tRNA ligases Human genes 0.000 description 3
- 108090000314 Asparagine-tRNA ligases Proteins 0.000 description 3
- 108010055400 Aspartate kinase Proteins 0.000 description 3
- 102000012951 Aspartate-tRNA Ligase Human genes 0.000 description 3
- 108010065272 Aspartate-tRNA ligase Proteins 0.000 description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 108091035707 Consensus sequence Proteins 0.000 description 3
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 3
- 230000033616 DNA repair Effects 0.000 description 3
- 108091000126 Dihydroorotase Proteins 0.000 description 3
- 102100030013 Endoribonuclease Human genes 0.000 description 3
- 101710199605 Endoribonuclease Proteins 0.000 description 3
- 101001130148 Escherichia coli (strain K12) D-lactate dehydrogenase Proteins 0.000 description 3
- 108010013942 GMP Reductase Proteins 0.000 description 3
- 102000017179 GMP reductase Human genes 0.000 description 3
- 108020000311 Glutamate Synthase Proteins 0.000 description 3
- 102000051366 Glycosyltransferases Human genes 0.000 description 3
- 108010064711 Homoserine dehydrogenase Proteins 0.000 description 3
- 108010087227 IMP Dehydrogenase Proteins 0.000 description 3
- 102000006674 IMP dehydrogenase Human genes 0.000 description 3
- 108030004616 Lipopolysaccharide N-acetylglucosaminyltransferases Proteins 0.000 description 3
- 241001302042 Methanothermobacter thermautotrophicus Species 0.000 description 3
- 108060004795 Methyltransferase Proteins 0.000 description 3
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 3
- 108010031683 PAPS sulfotransferase Proteins 0.000 description 3
- 108010010677 Phosphodiesterase I Proteins 0.000 description 3
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 3
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 3
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 description 3
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 description 3
- 241000522615 Pyrococcus horikoshii Species 0.000 description 3
- 101710113029 Serine/threonine-protein kinase Proteins 0.000 description 3
- 108010006785 Taq Polymerase Proteins 0.000 description 3
- 102000004243 Tubulin Human genes 0.000 description 3
- 108090000704 Tubulin Proteins 0.000 description 3
- 102100029640 UDP-glucose 6-dehydrogenase Human genes 0.000 description 3
- 108030001662 UDP-glucose 6-dehydrogenases Proteins 0.000 description 3
- 125000000539 amino acid group Chemical group 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 235000011148 calcium chloride Nutrition 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 description 3
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 description 3
- 108010042663 guanosine phosphotransferase Proteins 0.000 description 3
- 150000002484 inorganic compounds Chemical class 0.000 description 3
- 229910010272 inorganic material Inorganic materials 0.000 description 3
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 108091077527 major facilitator family Proteins 0.000 description 3
- 102000042200 major facilitator family Human genes 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- HPEUEJRPDGMIMY-IFQPEPLCSA-N molybdopterin Chemical compound O([C@H]1N2)[C@H](COP(O)(O)=O)C(S)=C(S)[C@@H]1NC1=C2N=C(N)NC1=O HPEUEJRPDGMIMY-IFQPEPLCSA-N 0.000 description 3
- 150000002894 organic compounds Chemical class 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 229940055619 selenocysteine Drugs 0.000 description 3
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 3
- 235000016491 selenocysteine Nutrition 0.000 description 3
- 108010050301 tRNA nucleotidyltransferase Proteins 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 238000010200 validation analysis Methods 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 2
- 101710192508 30S ribosomal protein S2 Proteins 0.000 description 2
- 108010070713 4-amino-5-(hydroxymethyl)-2-methylpyrimidine phosphate kinase Proteins 0.000 description 2
- 101000787132 Acidithiobacillus ferridurans Uncharacterized 8.2 kDa protein in mobL 3'region Proteins 0.000 description 2
- 101000827262 Acidithiobacillus ferrooxidans Uncharacterized 18.9 kDa protein in mobE 3'region Proteins 0.000 description 2
- 108010054404 Adenylyl-sulfate kinase Proteins 0.000 description 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 108010039224 Amidophosphoribosyltransferase Proteins 0.000 description 2
- 102100039239 Amidophosphoribosyltransferase Human genes 0.000 description 2
- 108010052434 Aminodeoxychorismate synthase Proteins 0.000 description 2
- 108010058065 Aminomethyltransferase Proteins 0.000 description 2
- 108010037870 Anthranilate Synthase Proteins 0.000 description 2
- 101000811747 Antithamnion sp. UPF0051 protein in atpA 3'region Proteins 0.000 description 2
- 108091023231 Ap4A Proteins 0.000 description 2
- 101000823228 Arabidopsis thaliana ABC transporter I family member 6, chloroplastic Proteins 0.000 description 2
- 101000666833 Autographa californica nuclear polyhedrosis virus Uncharacterized 20.8 kDa protein in FGF-VUBI intergenic region Proteins 0.000 description 2
- 101000977027 Azospirillum brasilense Uncharacterized protein in nodG 5'region Proteins 0.000 description 2
- 101000827607 Bacillus phage SPP1 Uncharacterized 8.5 kDa protein in GP2-GP6 intergenic region Proteins 0.000 description 2
- 101000961975 Bacillus thuringiensis Uncharacterized 13.4 kDa protein Proteins 0.000 description 2
- 101000962005 Bacillus thuringiensis Uncharacterized 23.6 kDa protein Proteins 0.000 description 2
- 101710110830 Beta-agarase Proteins 0.000 description 2
- 102100026189 Beta-galactosidase Human genes 0.000 description 2
- 102100032487 Beta-mannosidase Human genes 0.000 description 2
- 102100033743 Biotin-[acetyl-CoA-carboxylase] ligase Human genes 0.000 description 2
- 101000964407 Caldicellulosiruptor saccharolyticus Uncharacterized 10.7 kDa protein in xynB 3'region Proteins 0.000 description 2
- 102000003846 Carbonic anhydrases Human genes 0.000 description 2
- 108090000209 Carbonic anhydrases Proteins 0.000 description 2
- 108010078140 Cation Transport Proteins Proteins 0.000 description 2
- 108010003989 D-amino-acid oxidase Proteins 0.000 description 2
- 102000004674 D-amino-acid oxidase Human genes 0.000 description 2
- 108030000936 D-stereospecific aminopeptidases Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 102100034581 Dihydroorotase Human genes 0.000 description 2
- 101000785191 Drosophila melanogaster Uncharacterized 50 kDa protein in type I retrotransposable element R1DM Proteins 0.000 description 2
- 108700035791 EC 6.3.5.4 Proteins 0.000 description 2
- 101710081048 Endonuclease III Proteins 0.000 description 2
- 101000747704 Enterobacteria phage N4 Uncharacterized protein Gp1 Proteins 0.000 description 2
- 101000861206 Enterococcus faecalis (strain ATCC 700802 / V583) Uncharacterized protein EF_A0048 Proteins 0.000 description 2
- 101000703368 Escherichia coli (strain K12) L-asparaginase 2 Proteins 0.000 description 2
- 101000769180 Escherichia coli Uncharacterized 11.1 kDa protein Proteins 0.000 description 2
- 241001524679 Escherichia virus M13 Species 0.000 description 2
- 241001137858 Euryarchaeota Species 0.000 description 2
- 108010067157 Ferrichrome Proteins 0.000 description 2
- 108090000926 GMP synthase (glutamine-hydrolyzing) Proteins 0.000 description 2
- 102100033452 GMP synthase [glutamine-hydrolyzing] Human genes 0.000 description 2
- 108010055629 Glucosyltransferases Proteins 0.000 description 2
- 102000000340 Glucosyltransferases Human genes 0.000 description 2
- 102100023903 Glycerol kinase Human genes 0.000 description 2
- 108700016170 Glycerol kinases Proteins 0.000 description 2
- 102000004327 Glycine dehydrogenase (decarboxylating) Human genes 0.000 description 2
- 108090000826 Glycine dehydrogenase (decarboxylating) Proteins 0.000 description 2
- 108091069668 HIT family Proteins 0.000 description 2
- 102000040061 HIT family Human genes 0.000 description 2
- 101000768777 Haloferax lucentense (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2) Uncharacterized 50.6 kDa protein in the 5'region of gyrA and gyrB Proteins 0.000 description 2
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 101000607404 Infectious laryngotracheitis virus (strain Thorne V882) Protein UL24 homolog Proteins 0.000 description 2
- 108010009595 Inorganic Pyrophosphatase Proteins 0.000 description 2
- 101000735632 Klebsiella pneumoniae Uncharacterized 8.8 kDa protein in aacA4 3'region Proteins 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 2
- 101000976301 Leptospira interrogans Uncharacterized 35 kDa protein in sph 3'region Proteins 0.000 description 2
- 241000202974 Methanobacterium Species 0.000 description 2
- 102000005431 Molecular Chaperones Human genes 0.000 description 2
- 101000658690 Neisseria meningitidis serogroup B Transposase for insertion sequence element IS1106 Proteins 0.000 description 2
- 102000019040 Nuclear Antigens Human genes 0.000 description 2
- 108010051791 Nuclear Antigens Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 102100026367 Pancreatic alpha-amylase Human genes 0.000 description 2
- 108010090127 Periplasmic Proteins Proteins 0.000 description 2
- 108050008598 Phosphoesterases Proteins 0.000 description 2
- 102000009569 Phosphoglucomutase Human genes 0.000 description 2
- 102100036473 Phosphoribosylformylglycinamidine synthase Human genes 0.000 description 2
- 108030004873 Phosphoribosylformylglycinamidine synthases Proteins 0.000 description 2
- 102100021762 Phosphoserine phosphatase Human genes 0.000 description 2
- 101710101148 Probable 6-oxopurine nucleoside phosphorylase Proteins 0.000 description 2
- 101710150114 Protein rep Proteins 0.000 description 2
- 101000748660 Pseudomonas savastanoi Uncharacterized 21 kDa protein in iaaL 5'region Proteins 0.000 description 2
- 102000030764 Purine-nucleoside phosphorylase Human genes 0.000 description 2
- RADKZDMFGJYCBB-UHFFFAOYSA-N Pyridoxal Chemical compound CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
- 241000205156 Pyrococcus furiosus Species 0.000 description 2
- 108010023576 Pyruvate Dehydrogenase (Lipoamide) Proteins 0.000 description 2
- 102000011346 Pyruvate Dehydrogenase (Lipoamide) Human genes 0.000 description 2
- 108020005115 Pyruvate Kinase Proteins 0.000 description 2
- 102000013009 Pyruvate Kinase Human genes 0.000 description 2
- 230000004570 RNA-binding Effects 0.000 description 2
- 101710152114 Replication protein Proteins 0.000 description 2
- 102000046755 Ribokinases Human genes 0.000 description 2
- 101000584469 Rice tungro bacilliform virus (isolate Philippines) Protein P1 Proteins 0.000 description 2
- NAQGHJTUZRHGAC-ZZZDFHIKSA-N SAICAR Chemical compound NC1=C(C(=O)N[C@@H](CC(O)=O)C(O)=O)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 NAQGHJTUZRHGAC-ZZZDFHIKSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 2
- 101000703403 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) L-asparaginase 2-1 Proteins 0.000 description 2
- 101000703404 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) L-asparaginase 2-2 Proteins 0.000 description 2
- 101000901050 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) L-asparaginase 2-3 Proteins 0.000 description 2
- 101000901051 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) L-asparaginase 2-4 Proteins 0.000 description 2
- 108010022999 Serine Proteases Proteins 0.000 description 2
- 102000012479 Serine Proteases Human genes 0.000 description 2
- 102100026842 Serine-pyruvate aminotransferase Human genes 0.000 description 2
- 101000818100 Spirochaeta aurantia Uncharacterized 12.7 kDa protein in trpE 5'region Proteins 0.000 description 2
- 101000818096 Spirochaeta aurantia Uncharacterized 15.5 kDa protein in trpE 3'region Proteins 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 102100021685 Stomatin Human genes 0.000 description 2
- 108700037714 Stomatin Proteins 0.000 description 2
- 101000766081 Streptomyces ambofaciens Uncharacterized HTH-type transcriptional regulator in unstable DNA locus Proteins 0.000 description 2
- 101001037658 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) Glucokinase Proteins 0.000 description 2
- 239000012505 Superdex™ Substances 0.000 description 2
- 101000804403 Synechococcus elongatus (strain PCC 7942 / FACHB-805) Uncharacterized HIT-like protein Synpcc7942_1390 Proteins 0.000 description 2
- 101000750910 Synechococcus elongatus (strain PCC 7942 / FACHB-805) Uncharacterized HTH-type transcriptional regulator Synpcc7942_2319 Proteins 0.000 description 2
- 101000644897 Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) Uncharacterized protein SYNPCC7002_B0001 Proteins 0.000 description 2
- 241000204969 Thermococcales Species 0.000 description 2
- 102100037357 Thymidylate kinase Human genes 0.000 description 2
- 102000014701 Transketolase Human genes 0.000 description 2
- 108010043652 Transketolase Proteins 0.000 description 2
- 108010020764 Transposases Proteins 0.000 description 2
- 102000008579 Transposases Human genes 0.000 description 2
- 101710187777 Tryptophan synthase beta chain Proteins 0.000 description 2
- 108010075202 UDP-glucose 4-epimerase Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 102100039662 Xaa-Pro dipeptidase Human genes 0.000 description 2
- 101000916336 Xenopus laevis Transposon TX1 uncharacterized 82 kDa protein Proteins 0.000 description 2
- 101001000760 Zea mays Putative Pol polyprotein from transposon element Bs1 Proteins 0.000 description 2
- 101000678262 Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1) 65 kDa protein Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 102000011229 agmatinase Human genes 0.000 description 2
- 108091022872 aldose 1-epimerase Proteins 0.000 description 2
- RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 210000003578 bacterial chromosome Anatomy 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 108010055059 beta-Mannosidase Proteins 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 210000004899 c-terminal region Anatomy 0.000 description 2
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 2
- 229960003669 carbenicillin Drugs 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 125000004093 cyano group Chemical group *C#N 0.000 description 2
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 108010000742 dTMP kinase Proteins 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 108010088016 dolichyl-phosphate beta-D-mannosyltransferase Proteins 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 108091023030 exopolyphosphatase Proteins 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- GGUNGDGGXMHBMJ-UHFFFAOYSA-N ferrichrome Chemical compound [Fe+3].CC(=O)N([O-])CCCC1NC(=O)CNC(=O)CNC(=O)CNC(=O)C(CCCN([O-])C(C)=O)NC(=O)C(CCCN([O-])C(C)=O)NC1=O GGUNGDGGXMHBMJ-UHFFFAOYSA-N 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 108020001507 fusion proteins Proteins 0.000 description 2
- 102000037865 fusion proteins Human genes 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
- 238000009499 grossing Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229940025294 hemin Drugs 0.000 description 2
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 108010082612 homocitrate synthase Proteins 0.000 description 2
- 239000012456 homogeneous solution Substances 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 229920002113 octoxynol Polymers 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 2
- 108010076573 phosphoserine phosphatase Proteins 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 108010028138 prohibitin Proteins 0.000 description 2
- 102000016670 prohibitin Human genes 0.000 description 2
- 101150090832 recQ gene Proteins 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 108010060800 serine-pyruvate aminotransferase Proteins 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- VNOYUJKHFWYWIR-ITIYDSSPSA-N succinyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-ITIYDSSPSA-N 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- WTFXTQVDAKGDEY-UHFFFAOYSA-N (-)-chorismic acid Natural products OC1C=CC(C(O)=O)=CC1OC(=C)C(O)=O WTFXTQVDAKGDEY-UHFFFAOYSA-N 0.000 description 1
- BUHVIAUBTBOHAG-FOYDDCNASA-N (2r,3r,4s,5r)-2-[6-[[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)ethyl]amino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound COC1=CC(OC)=CC(C(CNC=2C=3N=CN(C=3N=CN=2)[C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)C=2C(=CC=CC=2)C)=C1 BUHVIAUBTBOHAG-FOYDDCNASA-N 0.000 description 1
- NFBQIWJDUKFHJP-SQOUGZDYSA-N (2r,3s,4r,5r)-3,4,5,6-tetrahydroxy-2-phosphonooxyhexanoic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C(O)=O)OP(O)(O)=O NFBQIWJDUKFHJP-SQOUGZDYSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- UWOCFOFVIBZJGH-UHFFFAOYSA-N 2,3-dihydrodipicolinic acid Chemical compound OC(=O)C1CC=CC(C(O)=O)=N1 UWOCFOFVIBZJGH-UHFFFAOYSA-N 0.000 description 1
- GMKMEZVLHJARHF-UHFFFAOYSA-N 2,6-diaminopimelic acid Chemical compound OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 1
- 108010058089 2-dehydro-3-deoxyphosphooctonate aldolase Proteins 0.000 description 1
- 108010048295 2-isopropylmalate synthase Proteins 0.000 description 1
- 108010018496 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase Proteins 0.000 description 1
- OINNEUNVOZHBOX-QIRCYJPOSA-K 2-trans,6-trans,10-trans-geranylgeranyl diphosphate(3-) Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C(C)=C\COP([O-])(=O)OP([O-])([O-])=O OINNEUNVOZHBOX-QIRCYJPOSA-K 0.000 description 1
- 108010080376 3-Deoxy-7-Phosphoheptulonate Synthase Proteins 0.000 description 1
- 108010038550 3-dehydroquinate dehydratase Proteins 0.000 description 1
- 102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 1
- 108010036575 3-methyl-2-oxobutanoate hydroxymethyltransferase Proteins 0.000 description 1
- 101710168866 3-oxoacyl-[acyl-carrier-protein] synthase 3 Proteins 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- 101710171187 30S ribosomal protein S10 Proteins 0.000 description 1
- 101710171221 30S ribosomal protein S11 Proteins 0.000 description 1
- 101710171220 30S ribosomal protein S12 Proteins 0.000 description 1
- 101710171219 30S ribosomal protein S13 Proteins 0.000 description 1
- 101710171217 30S ribosomal protein S15 Proteins 0.000 description 1
- 108050001922 30S ribosomal protein S17 Proteins 0.000 description 1
- 101710171175 30S ribosomal protein S19 Proteins 0.000 description 1
- 101710192511 30S ribosomal protein S3 Proteins 0.000 description 1
- 101710192513 30S ribosomal protein S4 Proteins 0.000 description 1
- 101710192514 30S ribosomal protein S5 Proteins 0.000 description 1
- 101710192510 30S ribosomal protein S7 Proteins 0.000 description 1
- 101710192522 30S ribosomal protein S8 Proteins 0.000 description 1
- 101710192523 30S ribosomal protein S9 Proteins 0.000 description 1
- 108010043797 4-alpha-glucanotransferase Proteins 0.000 description 1
- 102100033409 40S ribosomal protein S3 Human genes 0.000 description 1
- 108010075604 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase Proteins 0.000 description 1
- 102000011848 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase Human genes 0.000 description 1
- QUTYKIXIUDQOLK-PRJMDXOYSA-N 5-O-(1-carboxyvinyl)-3-phosphoshikimic acid Chemical compound O[C@H]1[C@H](OC(=C)C(O)=O)CC(C(O)=O)=C[C@H]1OP(O)(O)=O QUTYKIXIUDQOLK-PRJMDXOYSA-N 0.000 description 1
- PQGCEDQWHSBAJP-TXICZTDVSA-N 5-O-phosphono-alpha-D-ribofuranosyl diphosphate Chemical compound O[C@H]1[C@@H](O)[C@@H](O[P@](O)(=O)OP(O)(O)=O)O[C@@H]1COP(O)(O)=O PQGCEDQWHSBAJP-TXICZTDVSA-N 0.000 description 1
- PDACUKOKVHBVHJ-XVFCMESISA-N 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole Chemical compound NC1=CN=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 PDACUKOKVHBVHJ-XVFCMESISA-N 0.000 description 1
- XFVULMDJZXYMSG-ZIYNGMLESA-K 5-amino-1-(5-phosphonato-D-ribosyl)imidazole-4-carboxylate Chemical compound NC1=C(C([O-])=O)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP([O-])([O-])=O)O1 XFVULMDJZXYMSG-ZIYNGMLESA-K 0.000 description 1
- 101710190958 50S ribosomal protein L1 Proteins 0.000 description 1
- 101710135830 50S ribosomal protein L11 Proteins 0.000 description 1
- 101710135885 50S ribosomal protein L13 Proteins 0.000 description 1
- 101710135900 50S ribosomal protein L14 Proteins 0.000 description 1
- 101710135899 50S ribosomal protein L15 Proteins 0.000 description 1
- 101710135886 50S ribosomal protein L16 Proteins 0.000 description 1
- 101710135888 50S ribosomal protein L18 Proteins 0.000 description 1
- 101710190951 50S ribosomal protein L2 Proteins 0.000 description 1
- 101710135891 50S ribosomal protein L22 Proteins 0.000 description 1
- 101710135864 50S ribosomal protein L24 Proteins 0.000 description 1
- 101710190950 50S ribosomal protein L3 Proteins 0.000 description 1
- 101710190989 50S ribosomal protein L4 Proteins 0.000 description 1
- 101710190982 50S ribosomal protein L5 Proteins 0.000 description 1
- 108091027075 5S-rRNA precursor Proteins 0.000 description 1
- 102100038222 60 kDa heat shock protein, mitochondrial Human genes 0.000 description 1
- WBFYVDCHGVNRBH-UHFFFAOYSA-N 7,8-dihydropteroic acid Chemical compound N=1C=2C(=O)NC(N)=NC=2NCC=1CNC1=CC=C(C(O)=O)C=C1 WBFYVDCHGVNRBH-UHFFFAOYSA-N 0.000 description 1
- PJWIPEXIFFQAQZ-PUFIMZNGSA-N 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonic acid Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)CC(=O)C(O)=O PJWIPEXIFFQAQZ-PUFIMZNGSA-N 0.000 description 1
- 108010031096 8-amino-7-oxononanoate synthase Proteins 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 108010006229 Acetyl-CoA C-acetyltransferase Proteins 0.000 description 1
- 102100021641 Acetyl-CoA carboxylase 2 Human genes 0.000 description 1
- 108010024223 Adenine phosphoribosyltransferase Proteins 0.000 description 1
- 102100029457 Adenine phosphoribosyltransferase Human genes 0.000 description 1
- 102100032534 Adenosine kinase Human genes 0.000 description 1
- 108020000543 Adenylate kinase Proteins 0.000 description 1
- 108700016258 Agmatinases Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108050004944 Alpha-glucan phosphorylases Proteins 0.000 description 1
- 102000004092 Amidohydrolases Human genes 0.000 description 1
- 108090000531 Amidohydrolases Proteins 0.000 description 1
- 102100039338 Aminomethyltransferase, mitochondrial Human genes 0.000 description 1
- 102100040894 Amylo-alpha-1,6-glucosidase Human genes 0.000 description 1
- 101100010506 Arabidopsis thaliana DUF6 gene Proteins 0.000 description 1
- 101000640990 Arabidopsis thaliana Tryptophan-tRNA ligase, chloroplastic/mitochondrial Proteins 0.000 description 1
- 101000787278 Arabidopsis thaliana Valine-tRNA ligase, chloroplastic/mitochondrial 2 Proteins 0.000 description 1
- 101000787296 Arabidopsis thaliana Valine-tRNA ligase, mitochondrial 1 Proteins 0.000 description 1
- 102000004452 Arginase Human genes 0.000 description 1
- 108700024123 Arginases Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 102000002249 Arginine-tRNA Ligase Human genes 0.000 description 1
- 108010014885 Arginine-tRNA ligase Proteins 0.000 description 1
- 108010043325 Aryl-alcohol dehydrogenase Proteins 0.000 description 1
- 102000009133 Arylsulfatases Human genes 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 102000030907 Aspartate Carbamoyltransferase Human genes 0.000 description 1
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 1
- 101100137463 Bacillus subtilis (strain 168) ppsA gene Proteins 0.000 description 1
- 108050003866 Bifunctional ligase/repressor BirA Proteins 0.000 description 1
- 101710180532 Biotin carboxyl carrier protein of acetyl-CoA carboxylase Proteins 0.000 description 1
- 108010018763 Biotin carboxylase Proteins 0.000 description 1
- 108090000732 Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) Proteins 0.000 description 1
- 102100036461 Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical] Human genes 0.000 description 1
- 108010029692 Bisphosphoglycerate mutase Proteins 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 102100039866 CTP synthase 1 Human genes 0.000 description 1
- 101100360207 Caenorhabditis elegans rla-1 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 1
- 108010006303 Carboxypeptidases Proteins 0.000 description 1
- 102000005367 Carboxypeptidases Human genes 0.000 description 1
- 108010058432 Chaperonin 60 Proteins 0.000 description 1
- 108050001186 Chaperonin Cpn60 Proteins 0.000 description 1
- 102000052603 Chaperonins Human genes 0.000 description 1
- 108010062745 Chloride Channels Proteins 0.000 description 1
- 102000011045 Chloride Channels Human genes 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 241001137853 Crenarchaeota Species 0.000 description 1
- 101710095468 Cyclase Proteins 0.000 description 1
- 108050006400 Cyclin Proteins 0.000 description 1
- 108010076010 Cystathionine beta-lyase Proteins 0.000 description 1
- 108030001879 Cystathionine gamma-synthases Proteins 0.000 description 1
- 102000004403 Cysteine-tRNA ligases Human genes 0.000 description 1
- 108090000918 Cysteine-tRNA ligases Proteins 0.000 description 1
- 102000018832 Cytochromes Human genes 0.000 description 1
- 108010052832 Cytochromes Proteins 0.000 description 1
- 102000000311 Cytosine Deaminase Human genes 0.000 description 1
- 108010080611 Cytosine Deaminase Proteins 0.000 description 1
- 108020003338 D-alanine-D-alanine ligase Proteins 0.000 description 1
- 101710116957 D-alanyl-D-alanine carboxypeptidase Proteins 0.000 description 1
- UZYFNQCWJLIAKE-ZMIZWQJLSA-N D-arabino-hex-3-ulose 6-phosphate Chemical compound OC[C@@H](O)C(=O)[C@H](O)[C@H](O)COP(O)(O)=O UZYFNQCWJLIAKE-ZMIZWQJLSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102000016559 DNA Primase Human genes 0.000 description 1
- 108010092681 DNA Primase Proteins 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 102100029995 DNA ligase 1 Human genes 0.000 description 1
- 230000008836 DNA modification Effects 0.000 description 1
- 102000005768 DNA-Activated Protein Kinase Human genes 0.000 description 1
- 108010006124 DNA-Activated Protein Kinase Proteins 0.000 description 1
- 101000874334 Dalbergia nigrescens Isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase Proteins 0.000 description 1
- 101710113954 Deoxyguanosinetriphosphate triphosphohydrolase Proteins 0.000 description 1
- 108010036364 Deoxyribonuclease IV (Phage T4-Induced) Proteins 0.000 description 1
- 108700016241 Deoxyribose-phosphate aldolases Proteins 0.000 description 1
- 108010077448 Diamine N-acetyltransferase Proteins 0.000 description 1
- 101000787280 Dictyostelium discoideum Probable valine-tRNA ligase, mitochondrial Proteins 0.000 description 1
- 102000004860 Dipeptidases Human genes 0.000 description 1
- 108090001081 Dipeptidases Proteins 0.000 description 1
- 101710177360 Dolichol-phosphate mannosyltransferase Proteins 0.000 description 1
- 108700015360 Drosophila Rho Proteins 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 102100021160 Dual specificity protein phosphatase 9 Human genes 0.000 description 1
- 108700035993 EC 3.4.21.53 Proteins 0.000 description 1
- 102100027094 Echinoderm microtubule-associated protein-like 1 Human genes 0.000 description 1
- 102100031334 Elongation factor 2 Human genes 0.000 description 1
- 101000757733 Enterococcus faecalis (strain ATCC 700802 / V583) Autolysin Proteins 0.000 description 1
- 101100106377 Escherichia coli (strain K12) yjhR gene Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 108020005637 FAD dependent oxidoreductase Proteins 0.000 description 1
- 102000007384 FAD-dependent oxidoreductase Human genes 0.000 description 1
- 101001076781 Fructilactobacillus sanfranciscensis (strain ATCC 27651 / DSM 20451 / JCM 5668 / CCUG 30143 / KCTC 3205 / NCIMB 702811 / NRRL B-3934 / L-12) Ribose-5-phosphate isomerase A Proteins 0.000 description 1
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 1
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 1
- 102000048120 Galactokinases Human genes 0.000 description 1
- 108700023157 Galactokinases Proteins 0.000 description 1
- 101710198928 Gamma-glutamyl phosphate reductase Proteins 0.000 description 1
- 229920002148 Gellan gum Polymers 0.000 description 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 1
- OINNEUNVOZHBOX-XBQSVVNOSA-N Geranylgeranyl diphosphate Natural products [P@](=O)(OP(=O)(O)O)(OC/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)O OINNEUNVOZHBOX-XBQSVVNOSA-N 0.000 description 1
- 102000005133 Glutamate 5-kinase Human genes 0.000 description 1
- 108091022930 Glutamate decarboxylase Proteins 0.000 description 1
- 102000008214 Glutamate decarboxylase Human genes 0.000 description 1
- 102000016901 Glutamate dehydrogenase Human genes 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 102000017722 Glutamine amidotransferases Human genes 0.000 description 1
- 108050005901 Glutamine amidotransferases Proteins 0.000 description 1
- 102100024977 Glutamine-tRNA ligase Human genes 0.000 description 1
- 102000001861 Glutamyl-tRNA synthetases Human genes 0.000 description 1
- 108050009136 Glutamyl-tRNA synthetases Proteins 0.000 description 1
- 102100036596 Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial Human genes 0.000 description 1
- 108010085321 Glycine Decarboxylase Complex Proteins 0.000 description 1
- 102000007480 Glycine Decarboxylase Complex Human genes 0.000 description 1
- 102000001539 Glycine cleavage system T proteins Human genes 0.000 description 1
- 108010051724 Glycine-tRNA Ligase Proteins 0.000 description 1
- 102000019220 Glycyl-tRNA synthetases Human genes 0.000 description 1
- 102000004447 HSP40 Heat-Shock Proteins Human genes 0.000 description 1
- 108010042283 HSP40 Heat-Shock Proteins Proteins 0.000 description 1
- 101710113864 Heat shock protein 90 Proteins 0.000 description 1
- 102100034051 Heat shock protein HSP 90-alpha Human genes 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 102000029746 Histidine-tRNA Ligase Human genes 0.000 description 1
- 101710177011 Histidine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 108050003783 Histidinol-phosphate aminotransferase Proteins 0.000 description 1
- 101000677540 Homo sapiens Acetyl-CoA carboxylase 2 Proteins 0.000 description 1
- 101000652415 Homo sapiens Agmatinase, mitochondrial Proteins 0.000 description 1
- 101001057941 Homo sapiens Echinoderm microtubule-associated protein-like 1 Proteins 0.000 description 1
- 101001073064 Homo sapiens Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial Proteins 0.000 description 1
- 101000878213 Homo sapiens Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Proteins 0.000 description 1
- 101001012646 Homo sapiens Monoglyceride lipase Proteins 0.000 description 1
- 101001138544 Homo sapiens UMP-CMP kinase Proteins 0.000 description 1
- 102000004867 Hydro-Lyases Human genes 0.000 description 1
- 108090001042 Hydro-Lyases Proteins 0.000 description 1
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 1
- 102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100036984 Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Human genes 0.000 description 1
- 108010063678 Indole-3-Glycerol-Phosphate Synthase Proteins 0.000 description 1
- 102000009617 Inorganic Pyrophosphatase Human genes 0.000 description 1
- 102100027050 Inorganic pyrophosphatase Human genes 0.000 description 1
- 101710144867 Inositol monophosphatase Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 101710203526 Integrase Proteins 0.000 description 1
- 101710125507 Integrase/recombinase Proteins 0.000 description 1
- 102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
- 102000029793 Isoleucine-tRNA ligase Human genes 0.000 description 1
- 101710176147 Isoleucine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- SAUCHDKDCUROAO-VKHMYHEASA-N L-2-amino-3-oxobutanoic acid Chemical compound CC(=O)[C@H](N)C(O)=O SAUCHDKDCUROAO-VKHMYHEASA-N 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108010071170 Leucine-tRNA ligase Proteins 0.000 description 1
- 102100023342 Leucine-tRNA ligase, mitochondrial Human genes 0.000 description 1
- 108050005713 Lipoate-protein ligase A Proteins 0.000 description 1
- 101710115465 Lon protease Proteins 0.000 description 1
- 102100031955 Lon protease homolog, mitochondrial Human genes 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 1
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 1
- 108010087568 Mannosyltransferases Proteins 0.000 description 1
- 102000006722 Mannosyltransferases Human genes 0.000 description 1
- 241001074903 Methanobacteria Species 0.000 description 1
- 241000203353 Methanococcus Species 0.000 description 1
- 102100023174 Methionine aminopeptidase 2 Human genes 0.000 description 1
- 101710163328 Methionine synthase Proteins 0.000 description 1
- 102100031551 Methionine synthase Human genes 0.000 description 1
- 108010003060 Methionine-tRNA ligase Proteins 0.000 description 1
- 108090000192 Methionyl aminopeptidases Proteins 0.000 description 1
- 102000000362 Methionyl-tRNA synthetases Human genes 0.000 description 1
- 102100025825 Methylated-DNA-protein-cysteine methyltransferase Human genes 0.000 description 1
- 101710104913 Methylated-DNA-protein-cysteine methyltransferase Proteins 0.000 description 1
- 102000016397 Methyltransferase Human genes 0.000 description 1
- 108010059724 Micrococcal Nuclease Proteins 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 108010026899 Molybdopterin synthase Proteins 0.000 description 1
- 102100029814 Monoglyceride lipase Human genes 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101000653787 Mus musculus Protein S100-A11 Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 101000757734 Mycolicibacterium phlei 38 kDa autolysin Proteins 0.000 description 1
- BACYUWVYYTXETD-UHFFFAOYSA-N N-Lauroylsarcosine Chemical compound CCCCCCCCCCCC(=O)N(C)CC(O)=O BACYUWVYYTXETD-UHFFFAOYSA-N 0.000 description 1
- RFMMMVDNIPUKGG-YFKPBYRVSA-N N-acetyl-L-glutamic acid Chemical compound CC(=O)N[C@H](C(O)=O)CCC(O)=O RFMMMVDNIPUKGG-YFKPBYRVSA-N 0.000 description 1
- 108030003379 NAD(+) synthases Proteins 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 102000003832 Nucleotidyltransferases Human genes 0.000 description 1
- 108090000119 Nucleotidyltransferases Proteins 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 108010077524 Peptide Elongation Factor 1 Proteins 0.000 description 1
- 102000010292 Peptide Elongation Factor 1 Human genes 0.000 description 1
- 108010077519 Peptide Elongation Factor 2 Proteins 0.000 description 1
- 102100020739 Peptidyl-prolyl cis-trans isomerase FKBP4 Human genes 0.000 description 1
- VJNXVAVKCZJOFQ-UHFFFAOYSA-N Phenmetrazine hydrochloride Chemical compound Cl.CC1NCCOC1C1=CC=CC=C1 VJNXVAVKCZJOFQ-UHFFFAOYSA-N 0.000 description 1
- 102100035215 Phenylalanine-tRNA ligase alpha subunit Human genes 0.000 description 1
- 101710147128 Phenylalanine-tRNA ligase alpha subunit Proteins 0.000 description 1
- 102100035312 Phenylalanine-tRNA ligase beta subunit Human genes 0.000 description 1
- 101710182373 Phenylalanine-tRNA ligase beta subunit Proteins 0.000 description 1
- 101710110580 Phenylalanine-tRNA ligase beta subunit, chloroplastic Proteins 0.000 description 1
- 102000006335 Phosphate-Binding Proteins Human genes 0.000 description 1
- 108010058514 Phosphate-Binding Proteins Proteins 0.000 description 1
- 102100036629 Phosphoglucomutase-2 Human genes 0.000 description 1
- 101710138230 Phosphoglucomutase/phosphomannomutase Proteins 0.000 description 1
- 102000011025 Phosphoglycerate Mutase Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108010064209 Phosphoribosylglycinamide formyltransferase Proteins 0.000 description 1
- 102000015082 Phosphoribosylglycinamide formyltransferase Human genes 0.000 description 1
- 102000004257 Potassium Channel Human genes 0.000 description 1
- 101710096715 Probable histidine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 101710149031 Probable isoleucine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 101710113072 Probable methionine synthase Proteins 0.000 description 1
- 101710202161 Probable phenylalanine-tRNA ligase alpha subunit Proteins 0.000 description 1
- 101710119935 Probable phenylalanine-tRNA ligase beta subunit Proteins 0.000 description 1
- 101710146427 Probable tyrosine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 102100036691 Proliferating cell nuclear antigen Human genes 0.000 description 1
- 102100026126 Proline-tRNA ligase Human genes 0.000 description 1
- 108700015930 Prolyl Oligopeptidases Proteins 0.000 description 1
- 102100023832 Prolyl endopeptidase FAP Human genes 0.000 description 1
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 description 1
- 102000015176 Proton-Translocating ATPases Human genes 0.000 description 1
- 108010039518 Proton-Translocating ATPases Proteins 0.000 description 1
- 108030005599 Pseudouridylate synthases Proteins 0.000 description 1
- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 description 1
- 101710132082 Pyrimidine/purine nucleoside phosphorylase Proteins 0.000 description 1
- 101100407249 Pyrococcus abyssi (strain GE5 / Orsay) pcn gene Proteins 0.000 description 1
- 101100064061 Pyrococcus abyssi (strain GE5 / Orsay) polI gene Proteins 0.000 description 1
- 101100087339 Pyrococcus abyssi (strain GE5 / Orsay) rfcL gene Proteins 0.000 description 1
- 101100468506 Pyrococcus abyssi (strain GE5 / Orsay) rfcS gene Proteins 0.000 description 1
- 101000883586 Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) Deglycase PfpI Proteins 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 1
- 108700020471 RNA-Binding Proteins Proteins 0.000 description 1
- 101150025379 RPA1 gene Proteins 0.000 description 1
- 108090001066 Racemases and epimerases Proteins 0.000 description 1
- 102000004879 Racemases and epimerases Human genes 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102100040756 Rhodopsin Human genes 0.000 description 1
- 108700006309 Ribokinases Proteins 0.000 description 1
- 102100038013 Ribonucleoside-diphosphate reductase subunit M2 B Human genes 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 102000007382 Ribose-5-phosphate isomerase Human genes 0.000 description 1
- 108010030161 Serine-tRNA ligase Proteins 0.000 description 1
- 102100040516 Serine-tRNA ligase, cytoplasmic Human genes 0.000 description 1
- 101710189648 Serine/threonine-protein phosphatase Proteins 0.000 description 1
- 108050008280 Shikimate dehydrogenase Proteins 0.000 description 1
- 108010051611 Signal Recognition Particle Proteins 0.000 description 1
- 102000013598 Signal recognition particle Human genes 0.000 description 1
- 108030002936 Signal-recognition-particle GTPases Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 102100021996 Staphylococcal nuclease domain-containing protein 1 Human genes 0.000 description 1
- 102100027659 Striated muscle preferentially expressed protein kinase Human genes 0.000 description 1
- 108010056371 Succinyl-diaminopimelate desuccinylase Proteins 0.000 description 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 102000006467 TATA-Box Binding Protein Human genes 0.000 description 1
- 108010044281 TATA-Box Binding Protein Proteins 0.000 description 1
- 101000711499 Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) N(1)-aminopropylagmatine ureohydrolase Proteins 0.000 description 1
- 102000002933 Thioredoxin Human genes 0.000 description 1
- 102000013090 Thioredoxin-Disulfide Reductase Human genes 0.000 description 1
- 108010079911 Thioredoxin-disulfide reductase Proteins 0.000 description 1
- 102000001618 Threonine-tRNA Ligase Human genes 0.000 description 1
- 108010029287 Threonine-tRNA ligase Proteins 0.000 description 1
- 102000013537 Thymidine Phosphorylase Human genes 0.000 description 1
- 101710183280 Topoisomerase Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 102000004408 Transcription factor TFIIB Human genes 0.000 description 1
- 108090000941 Transcription factor TFIIB Proteins 0.000 description 1
- 101710094267 Transcription termination factor Rho Proteins 0.000 description 1
- 108050005726 Translation elongation factor P Proteins 0.000 description 1
- 102100027059 Translation initiation factor eIF-2B subunit alpha Human genes 0.000 description 1
- 101710123247 Translation initiation factor eIF-2B subunit alpha Proteins 0.000 description 1
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 1
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 101710151098 Tryptophan synthase alpha chain Proteins 0.000 description 1
- 102000002501 Tryptophan-tRNA Ligase Human genes 0.000 description 1
- 102000018378 Tyrosine-tRNA ligase Human genes 0.000 description 1
- 101710107268 Tyrosine-tRNA ligase, mitochondrial Proteins 0.000 description 1
- 101710091363 UDP-N-acetylglucosamine 2-epimerase Proteins 0.000 description 1
- 102100020797 UMP-CMP kinase Human genes 0.000 description 1
- 101710159648 Uncharacterized protein Proteins 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 102000013625 Valine-tRNA Ligase Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 108030005221 Xaa-Pro dipeptidases Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- JGGLZQUGOKVDGS-VYTIMWRQSA-N aspartate semialdehyde Chemical compound O[C@@H]1[C@@H](NC(=O)C)CO[C@H](CO)[C@H]1O[C@@H]1[C@@H](NC(C)=O)[C@H](O)[C@H](O[C@@H]2[C@H]([C@@H](O[C@@H]3[C@@H]([C@H](O)[C@@H](O)[C@H](CO)O3)O[C@@H]3[C@@H]([C@H](O)[C@@H](O)[C@H](CO)O3)O[C@@H]3[C@H]([C@H](O)[C@@H](O)[C@H](CO)O3)O)[C@@H](O)[C@H](CO[C@@H]3[C@H]([C@H](O[C@@H]4[C@H]([C@H](O)[C@@H](O)[C@H](CO)O4)O)[C@@H](O)[C@H](CO)O3)O)O2)O)[C@H](CO)O1 JGGLZQUGOKVDGS-VYTIMWRQSA-N 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 108010057307 butanol dehydrogenase Proteins 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000009134 cell regulation Effects 0.000 description 1
- 230000030570 cellular localization Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000035605 chemotaxis Effects 0.000 description 1
- WTFXTQVDAKGDEY-HTQZYQBOSA-L chorismate(2-) Chemical compound O[C@@H]1C=CC(C([O-])=O)=C[C@H]1OC(=C)C([O-])=O WTFXTQVDAKGDEY-HTQZYQBOSA-L 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 108010056332 correxonuclease Proteins 0.000 description 1
- 239000013601 cosmid vector Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 102000029589 dTDPglucose 4,6-dehydratase Human genes 0.000 description 1
- 108091000032 dTDPglucose 4,6-dehydratase Proteins 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 102000030794 deoxyribose-phosphate aldolase Human genes 0.000 description 1
- 230000030609 dephosphorylation Effects 0.000 description 1
- 238000006209 dephosphorylation reaction Methods 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- UFIVEPVSAGBUSI-UHFFFAOYSA-N dihydroorotic acid Chemical compound OC(=O)C1CC(=O)NC(=O)N1 UFIVEPVSAGBUSI-UHFFFAOYSA-N 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 150000002031 dolichols Chemical class 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 108010091371 endoglucanase 1 Proteins 0.000 description 1
- 108010091384 endoglucanase 2 Proteins 0.000 description 1
- 108010092450 endoglucanase Z Proteins 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- CJJCPDZKQKUXSS-JMSAOHGTSA-N fuculose Chemical compound C[C@@H]1OC(O)(CO)[C@H](O)[C@@H]1O CJJCPDZKQKUXSS-JMSAOHGTSA-N 0.000 description 1
- VZCYOOQTPOCHFL-OWOJBTEDSA-L fumarate(2-) Chemical compound [O-]C(=O)\C=C\C([O-])=O VZCYOOQTPOCHFL-OWOJBTEDSA-L 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
- 108010051239 glutaminyl-tRNA synthetase Proteins 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 102000006029 inositol monophosphatase Human genes 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 108020001983 isochorismate synthase Proteins 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 108010085781 maltodextrin phosphorylase Proteins 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- FQXKZRWLIJVZPF-UHFFFAOYSA-N marsin Natural products OC1C(O)C(O)C(C)OC1OC1CC2=CCC3C4(O)CCC(C(C)=O)C4(C)CCC3C2(C)CC1 FQXKZRWLIJVZPF-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 108010027581 nucleoside triphosphate pyrophosphatase Proteins 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- KYOBSHFOBAOFBF-XVFCMESISA-N orotidine 5'-phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1C(O)=O KYOBSHFOBAOFBF-XVFCMESISA-N 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 108010001722 phosphopentomutase Proteins 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 108020001213 potassium channel Proteins 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108010066823 proline dipeptidase Proteins 0.000 description 1
- 108010042589 prolyl T RNA synthetase Proteins 0.000 description 1
- 108020003519 protein disulfide isomerase Proteins 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 229960003581 pyridoxal Drugs 0.000 description 1
- 235000008164 pyridoxal Nutrition 0.000 description 1
- 239000011674 pyridoxal Substances 0.000 description 1
- 108010061997 queuine tRNA-ribosyltransferase Proteins 0.000 description 1
- 102000005499 queuine tRNA-ribosyltransferase Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000031267 regulation of DNA replication Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 108020005610 ribose 5-phosphate isomerase Proteins 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011451 sequencing strategy Methods 0.000 description 1
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 description 1
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- CIJQGPVMMRXSQW-UHFFFAOYSA-M sodium;2-aminoacetic acid;hydroxide Chemical compound O.[Na+].NCC([O-])=O CIJQGPVMMRXSQW-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000013179 statistical model Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 108060007951 sulfatase Proteins 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 101710083083 tRNA-splicing endonuclease Proteins 0.000 description 1
- 108010055693 tartronate-semialdehyde synthase Proteins 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229960002363 thiamine pyrophosphate Drugs 0.000 description 1
- 235000008170 thiamine pyrophosphate Nutrition 0.000 description 1
- 239000011678 thiamine pyrophosphate Substances 0.000 description 1
- YXVCLPJQTZXJLH-UHFFFAOYSA-N thiamine(1+) diphosphate chloride Chemical compound [Cl-].CC1=C(CCOP(O)(=O)OP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N YXVCLPJQTZXJLH-UHFFFAOYSA-N 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 108060008226 thioredoxin Proteins 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- WBYWAXJHAXSJNI-VOTSOKGWSA-N trans-cinnamic acid Chemical compound OC(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-N 0.000 description 1
- 102000014898 transaminase activity proteins Human genes 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000005829 trimerization reaction Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 229940035936 ubiquinone Drugs 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 108010063664 uroporphyrin-III C-methyltransferase Proteins 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9905034A FR2792651B1 (fr) | 1999-04-21 | 1999-04-21 | Sequence genomique et polypeptides de pyrococcus abyssi, leurs fragments et leurs utilisations |
| FR9905034 | 1999-04-21 | ||
| PCT/FR2000/001065 WO2000065062A2 (fr) | 1999-04-21 | 2000-04-21 | Sequence genomique et polypeptides de pyrococcus abissy, leurs fragments et leurs utilisations |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60029325D1 DE60029325D1 (en) | 2006-08-24 |
| DE60029325T2 true DE60029325T2 (de) | 2007-03-08 |
Family
ID=9544691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60029325T Expired - Lifetime DE60029325T2 (de) | 1999-04-21 | 2000-04-21 | Typ ii dns polymerase aus pyrococcus abyssi |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1196583B1 (OSRAM) |
| JP (1) | JP2004500802A (OSRAM) |
| AT (1) | ATE332971T1 (OSRAM) |
| AU (1) | AU4302000A (OSRAM) |
| CA (1) | CA2371253A1 (OSRAM) |
| DE (1) | DE60029325T2 (OSRAM) |
| FR (1) | FR2792651B1 (OSRAM) |
| WO (1) | WO2000065062A2 (OSRAM) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11829886B2 (en) | 2018-03-07 | 2023-11-28 | International Business Machines Corporation | Epistemic and aleatoric deep plasticity based on sound feedback |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2602183C (en) | 2005-03-18 | 2014-05-06 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| EP2078092A2 (en) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| GB201918108D0 (en) * | 2019-12-10 | 2020-01-22 | Vib Vzw | Improved aminopeptiadases for single molecule peptide sequencing |
| WO2021163559A1 (en) * | 2020-02-13 | 2021-08-19 | New England Biolabs, Inc. | Variant family d dna polymerases |
| EP4103701A1 (en) * | 2020-02-13 | 2022-12-21 | New England Biolabs, Inc. | Variant family d dna polymerases |
| WO2024006783A2 (en) * | 2022-06-30 | 2024-01-04 | Illumina, Inc. | Methylation detection with a non-natural/unnatural base |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0870832A4 (en) * | 1995-12-27 | 2001-08-22 | Takara Shuzo Co | NEW DNA POLYMERASE |
| FR2750999B1 (fr) * | 1996-07-10 | 1998-11-20 | Appligene Oncor | Adn polymerase thermostable d'archaebacteries du genre pyrococcus sp |
| EP0997530B1 (en) * | 1997-06-26 | 2006-08-23 | Takara Bio Inc. | Dna polymerase-related factors |
| WO2000008164A2 (de) * | 1998-08-06 | 2000-02-17 | Lion Bioscience Ag | Thermostabiler in vitro-komplex mit polymeraseaktivität |
-
1999
- 1999-04-21 FR FR9905034A patent/FR2792651B1/fr not_active Expired - Fee Related
-
2000
- 2000-04-21 JP JP2000614397A patent/JP2004500802A/ja active Pending
- 2000-04-21 WO PCT/FR2000/001065 patent/WO2000065062A2/fr not_active Ceased
- 2000-04-21 AU AU43020/00A patent/AU4302000A/en not_active Abandoned
- 2000-04-21 DE DE60029325T patent/DE60029325T2/de not_active Expired - Lifetime
- 2000-04-21 CA CA002371253A patent/CA2371253A1/fr not_active Abandoned
- 2000-04-21 AT AT00922717T patent/ATE332971T1/de not_active IP Right Cessation
- 2000-04-21 EP EP00922717A patent/EP1196583B1/fr not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11829886B2 (en) | 2018-03-07 | 2023-11-28 | International Business Machines Corporation | Epistemic and aleatoric deep plasticity based on sound feedback |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE332971T1 (de) | 2006-08-15 |
| EP1196583A2 (fr) | 2002-04-17 |
| FR2792651A1 (fr) | 2000-10-27 |
| CA2371253A1 (fr) | 2000-11-02 |
| DE60029325D1 (en) | 2006-08-24 |
| EP1196583B1 (fr) | 2006-07-12 |
| WO2000065062A2 (fr) | 2000-11-02 |
| WO2000065062A9 (fr) | 2002-09-06 |
| JP2004500802A (ja) | 2004-01-15 |
| WO2000065062A3 (fr) | 2002-02-14 |
| AU4302000A (en) | 2000-11-10 |
| FR2792651B1 (fr) | 2005-03-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vega‐Palas et al. | NtcA, a global nitrogen regulator from the cyanobacterium Synechococcus that belongs to the Crp family of bacterial regulators | |
| US20070020625A1 (en) | Sequence of the photorhabdus luminescens strain tt01 genome and uses | |
| Van Ham et al. | Putative evolutionary origin of plasmids carrying the genes involved in leucine biosynthesis in Buchnera aphidicola (endosymbiont of aphids) | |
| CN102080062B (zh) | L-半胱氨酸生产菌以及l-半胱氨酸的生产方法 | |
| WO2004022736A1 (ja) | 遺伝子のターゲティング破壊法および超耐熱菌ゲノム、ならびにこれらを利用したゲノムチップ | |
| Kim et al. | The MAL63 gene of Saccharomyces encodes a cysteine-zinc finger protein | |
| Wright et al. | Form species Nostoc commune (Cyanobacteria). | |
| DE60029325T2 (de) | Typ ii dns polymerase aus pyrococcus abyssi | |
| JP2004236637A (ja) | 多糖類生成に関与する遺伝子及びその利用 | |
| US20030134302A1 (en) | Libraries of expressible gene sequences | |
| US6921813B1 (en) | DNA Molecules | |
| Burgos et al. | Deletion of the Mycoplasma genitalium MG_217 gene modifies cell gliding behaviour by altering terminal organelle curvature | |
| Coates et al. | The hermit transposable element of the Australian sheep blowfly, Lucilia cuprina, belongs to the hAT family of transposable elements | |
| WO2003093434A2 (en) | Nanoarchaeum nucleic acid molecule | |
| Warren et al. | The Australian bushfly Musca vetustissima contains a sequence related to transposons of the hobo, Ac and Tam3 family | |
| Wahlund et al. | Expressed sequence tag profiles from calcifying and non-calcifying cultures of Emiliania huxleyi | |
| WO2006018205A2 (de) | Neue essentielle gene von bacillus licheniformis und darauf aufbauende verbesserte biotechnologische produktionsverfahren | |
| Sunil et al. | In Silico Characterization of a Transcript Code Based Screening of Antimicrobial Peptide from Trichogramma chilonis | |
| WO2022155445A1 (en) | Non-naturally occurring host cells for enhanced plant growth | |
| Qi et al. | Complete mitochondrial genome sequence of the humphead wrasse, Cheilinus undulatus | |
| Kim et al. | The complete mitochondrial genome of Koerneria sudhausi (Diplogasteromorpha: Nematoda) supports monophyly of Diplogasteromorpha within Rhabditomorpha | |
| Frommer et al. | Sequence from Eye Colour Genes, Chorion Gene and Mariner-Like Transposable Elements in the Queensland Fruit Fly, Bactrocera tryoni | |
| CN119331066B (zh) | 一种小麦分泌载体相关膜蛋白TaSCAMP6及其应用 | |
| WO2007099737A1 (ja) | Dnaポリメラーゼ変異体 | |
| JP3103881B2 (ja) | プラスミド |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: IFREMER INSTITUT FRANCAIS DE RECHERCHE POUR L', FR |