DE567365C - Process for obtaining the growth-promoting substances of the thymus glands in purified, water-soluble form - Google Patents
Process for obtaining the growth-promoting substances of the thymus glands in purified, water-soluble formInfo
- Publication number
- DE567365C DE567365C DE1930567365D DE567365DD DE567365C DE 567365 C DE567365 C DE 567365C DE 1930567365 D DE1930567365 D DE 1930567365D DE 567365D D DE567365D D DE 567365DD DE 567365 C DE567365 C DE 567365C
- Authority
- DE
- Germany
- Prior art keywords
- water
- growth
- purified
- obtaining
- promoting substances
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
Verfahren zur Gewinnung der wachstumfördernden Stoffe der Thymusdrüsen in gereinigter, wasserlöslicher Form Es ist bekannt, daß sich aus Thymusdrüsen wachstumfördernde Stoffe in wasserlöslicher Form durch Extraktion gewinnen lassen.Process for obtaining the growth-promoting substances of the thymus glands in purified, water-soluble form It is known that thymus glands produce growth-promoting glands Obtain substances in water-soluble form by extraction.
Es wurde nun gefunden, daß man durch Fällung eiweißfreier, wässeriger Lösungen solcher Stoffe mit wasserlöslichen organischen Lösungsmitteln, wie z. B. Methyl- oder Äthylalkohol, Aceton usw., eine weitere Reinigung erzielen kann. Die Hormone gehen nahezu quantitativ in den Niederschlag über. Durch wiederholtes Auflösen in Wasser und Fällen mit dem gleichen oder einem andern wasserlöslichen Lösungsmittel wird die Abtrennung der unwirksamen Bestandteile vervollständigt.It has now been found that protein-free, more watery ones can be obtained by precipitation Solutions of such substances with water-soluble organic solvents, such as. B. Methyl or ethyl alcohol, acetone, etc., can achieve further purification. the Hormones are almost quantitatively transferred to the precipitate. By repeatedly dissolving in water and cases with the same or a different water-soluble solvent the separation of the ineffective components is completed.
Nach dem Trocknen erhält man die wachstumfördernden Stoffe in Form eines hellgelben Pulvers, das in Wasser leicht, in den meisten organischen Lösungsmitteln aber schwer löslich ist.After drying, the growth-promoting substances are obtained in shape a light yellow powder that dissolves easily in water in most organic solvents but is difficult to dissolve.
Besonders geeignete Ausgangsstoffe werden z. B. nach einem der beiden folgenden Verfahren erhalten: a) Frisch bereiteter Thymusbrei wird mit wasserlöslichen Alkoholen ausgezogen, das Filtrat eingedampft, der Rückstand gleichzeitig oder abwechslungs-,veise mit Wasser und mit einem mit Wasser nicht mischbaren Lösungsmittel bis zur Lösung behandelt, die wässerige Schicht abgetrennt, darin noch enthaltenes Eiweiß in bekannter Weise ausgefällt und das klare, wässerige Filtrat erforderlichenfalls zur Trockne verdampft.Particularly suitable starting materials are, for. B. after one of the two The following procedure is obtained: a) Freshly prepared thymus pulp is made with water-soluble Alcohols extracted, the filtrate evaporated, the residue at the same time or alternately, veise with water and with a water-immiscible solvent until it dissolves treated, the aqueous layer separated, the protein still contained in it in known Wise precipitated and the clear, aqueous filtrate, if necessary, to dryness evaporates.
b) Zerkleinerte Thymusdrüsen werden vorerst zur Entfernung wachstumhemmender Stoffe und anderer Verunreinigungen nacheinander mit Aceton und mit einem mit Wasser nicht mischbaren -organischen Lösungsmittel, wie Äther, extrahiert; hierauf zieht man die wachstumfördernden Stoffe mit Wasser aus, fällt die Eiweißstoffe nach bekannten Methoden, entfernt den überschuß des Fällungsmittels und dampft gegebenenfalls die wässerige Lösung ein.b) Shredded thymus glands are initially growth-inhibiting for removal Substances and other contaminants one after the other with acetone and one with water immiscible organic solvents, such as ether, extracted; then pulls if the growth-promoting substances are eliminated with water, the protein substances are precipitated according to known ones Methods, removed the excess of the precipitant and, if necessary, steamed the aqueous solution.
Beispiel 30 g der nach dem oben beschriebenen Verfahren erhaltenen wasserlöslichen Stoffe aus Thymusdrüsen werden zu 5o ccm in Wasser gelöst. Nach Zusatz des sechsfachen Volumens g5°/oigen Alkohols und kräftigem Schütteln bildet sich ein Niederschlag, von welchem die klare, wässerige, alkoholische Lösung abgegossen wird. Der Rückstand wird erneut in Wasser gelöst. die Lösung auf 6o ccm verdünnt und mit dem sechsfachen Vo- Lumen 95°foigen Alkohols versetzt. Nach dem Abgießen der Flüssigkeit wird der Niederschlag im Vakuum getrocknet. Er enthält die iwachstumfördernden Stoffe in gereinigter Form. EXAMPLE 30 g of the water-soluble substances obtained from the thymus glands by the process described above are dissolved in water at 50 cc. After adding six times the volume of 5% alcohol and shaking vigorously, a precipitate forms, from which the clear, aqueous, alcoholic solution is poured off. The residue is redissolved in water. the solution is diluted to 60 cc and six times the volume of 95% alcohol is added. After the liquid has been poured off, the precipitate is dried in vacuo. It contains the substances that promote growth in a purified form.
Claims (1)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE567365T | 1930-10-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE567365C true DE567365C (en) | 1932-12-31 |
Family
ID=34584582
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE1930567365D Expired DE567365C (en) | 1930-10-30 | 1930-10-30 | Process for obtaining the growth-promoting substances of the thymus glands in purified, water-soluble form |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE567365C (en) |
-
1930
- 1930-10-30 DE DE1930567365D patent/DE567365C/en not_active Expired
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