DE557664C - Process for obtaining growth-promoting, water-soluble substances from thymus glands - Google Patents

Process for obtaining growth-promoting, water-soluble substances from thymus glands

Info

Publication number
DE557664C
DE557664C DE1930557664D DE557664DD DE557664C DE 557664 C DE557664 C DE 557664C DE 1930557664 D DE1930557664 D DE 1930557664D DE 557664D D DE557664D D DE 557664DD DE 557664 C DE557664 C DE 557664C
Authority
DE
Germany
Prior art keywords
water
promoting
growth
substances
soluble substances
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE1930557664D
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemische Ind Ges
GESELLSCHAFT fur CHEMISCHE INDUSTRIE
BASF Schweiz AG
Original Assignee
Chemische Ind Ges
GESELLSCHAFT fur CHEMISCHE INDUSTRIE
Gesellschaft fuer Chemische Industrie in Basel CIBA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE557664T external-priority
Application filed by Chemische Ind Ges, GESELLSCHAFT fur CHEMISCHE INDUSTRIE, Gesellschaft fuer Chemische Industrie in Basel CIBA filed Critical Chemische Ind Ges
Application granted granted Critical
Publication of DE557664C publication Critical patent/DE557664C/en
Expired legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes

Description

Verfahren zur Gewinnung wachstumfördernder, wasserlöslicher Stoffe aus Thymusdrüsen Es ist bereits bekannt, daß Thyrnusdrüsen sowohl wachstumhemmende wie auch wachstumfördernde Stoffe enthalten. Es wurde nun gefunden, daB man die wachstumfördernden Stoffe in wasserlöslicher Form vorteilhaft gewinnen kann, indem man die frisch zerkleinerten Thymusdrüsen vorerst zur Abtrennung der wachstumhemmenden Stoffe sowie von Verunreinigungen nacheinander mit Aceton und mit Wasser nicht mischbaren Lösungsmitteln, wie z. B. Äther oder Benzol, extrahiert, hierauf den Rückstand mit Wasser auszieht und den Auszug mit Eiweißfällungsmitteln, wie z. B. Essigsäure, behandelt. Nach Entfernung des überschüssigen Fällungsproduktes erhält man eine klare, wässerige Lösung der wachstumfördernden Stoffe, die direkt für Injektionszwecke Verwendung finden kann. Durch Vertreibung des Wassers gewinnt man die wirksamen Stoffe in fester Form. Beispiel r kg frisch zerkleinerte Tymusdrüsen werden während 2q. Stunden mit Aceton aasgerührt, abgenutscht und nach Vertreiben des anhaftenden Lösungsmittels in gleicher Weise mit Äther behandelt. Hierauf wird der Extraktionsrückstand 24 Stunden lang mit Wasser extrahiert und das wässerige Extrakt im Vakuum zur Trockne verdampft. Der Verdampfungsrückstand wird in etwa 31 Wasser gelöst und mit so viel Essigsäure versetzt, bis in der Hitze alles Eiweiß ausgefallen ist. Aus dem Filtrat wird durch Eindampfen im Vakuum die wirksame Substanz gewonnen.Process for the extraction of growth-promoting, water-soluble substances from thymus glands It is already known that thyrnus glands are both growth retardant as well as contain growth-promoting substances. It has now been found that the Growth-promoting substances in water-soluble form can advantageously be obtained by the freshly minced thymus glands are initially used to separate the growth-inhibiting glands Substances and impurities are not miscible with acetone and water one after the other Solvents such as B. ether or benzene, extracted, then the residue with Water pulls out and the extract with protein precipitants, such as. B. acetic acid, treated. After removing the excess precipitate, one obtains a clear, aqueous solution of growth-promoting substances, directly for injection purposes Can be used. The effective ones are obtained by expelling the water Substances in solid form. Example r kg of freshly minced tymus glands are during 2q. Stirred with acetone for hours, suction filtered and after expelling the adhering Solvent treated in the same way with ether. This is where the extraction residue becomes Extracted with water for 24 hours and the aqueous extract to dryness in vacuo evaporates. The evaporation residue is dissolved in about 31 water and with as much Acetic acid is added until all the protein has precipitated in the heat. From the filtrate the active substance is obtained by evaporation in a vacuum.

Claims (1)

PATENTANSPRUCH: Verfahren zur Gewinnung wachstumfördernder wasserlöslicher Stoffe aus Thymusdrüsen mit Hilfe organischer Lösungsmittel, dadurch gekennzeichnet, daß man die frisch zerkleinerten Organe vorerst zur Entfernung wachstumhemmender Stoffe und anderer Verunreinigungen nacheinander mit Aceton und mit mit Wasser nicht mischbaren organischen Lösungsmitteln extrahiert, hierauf die wachtumfördernden Stoffe mit Wasser auszieht, die Eiweißstoffe nach bekannten Methoden ausfällt, den überschuß des Fällungsmittels entfernt und gegebenenfalls die wässerige Lösung eindampft. PATENT CLAIM: Process for obtaining growth-promoting, water-soluble substances from thymus glands with the aid of organic solvents, characterized in that the freshly minced organs are first extracted one after the other with acetone and with water-immiscible organic solvents to remove growth-inhibiting substances and with organic solvents that are immiscible with water, then the growth-promoting substances are extracted with it The water is extracted, the protein is precipitated by known methods, the excess of the precipitating agent is removed and, if necessary, the aqueous solution is evaporated.
DE1930557664D 1930-07-20 1930-07-20 Process for obtaining growth-promoting, water-soluble substances from thymus glands Expired DE557664C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE557664T 1930-07-20

Publications (1)

Publication Number Publication Date
DE557664C true DE557664C (en) 1932-08-26

Family

ID=6565153

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1930557664D Expired DE557664C (en) 1930-07-20 1930-07-20 Process for obtaining growth-promoting, water-soluble substances from thymus glands

Country Status (1)

Country Link
DE (1) DE557664C (en)

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