DE479304C - Device for breeding microorganisms in the absence of oxygen - Google Patents
Device for breeding microorganisms in the absence of oxygenInfo
- Publication number
- DE479304C DE479304C DEZ16176D DEZ0016176D DE479304C DE 479304 C DE479304 C DE 479304C DE Z16176 D DEZ16176 D DE Z16176D DE Z0016176 D DEZ0016176 D DE Z0016176D DE 479304 C DE479304 C DE 479304C
- Authority
- DE
- Germany
- Prior art keywords
- oxygen
- lid
- absence
- microorganisms
- shell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
Vorrichtung zur Züchtung von Mikroorganismen unter Sauerstoffabschluß Die Vorrichtung besteht aus zwei Teilen z. aus einer flachen kreisrunden Schale a von etwa xo cm Durchmesser aus durchsichtigem Glase mit ebenem Boden und einer senkrechten Wandung b von etwa 1,6 cm Höhe; 2. aus einem Deckel c, ebenfalls aus durchsichtigemGlase und von gleichem äußerenDurchmesser und gleicher Wandstärke wie die Schale a. Die senkrechte Wand d des Deckels c kann beliebig hoch gewählt werden; die Höhe richtet sich nach der Menge der zur Absorption nötigen chemischen Substanzen. Im allgemeinen wird sie etwa 1,2 cm hoch sein müssen. Die Wand d hat eine horizontale Umbiegung, und zwar so weit, daß der Deckel c auf der Seitenwand b der Schale a eine Auflage finden kann. Nach der Umbiegung setzt sich die Wand d wieder senkrecht nach unten in den Wandteil e fort, macht eine neue horizontale Umbiegung und wird dann senkrecht nach oben mit dem Teil f bis etwa 2 mm an die Deckelfläche herangeführt. Auf diese Weise ist innerhalb des Deckels eine Rinne g gebildet, die rings um den Deckel herumläuft.Device for cultivating microorganisms in the absence of oxygen. from a flat circular bowl a about xo cm in diameter made of transparent glass with a flat bottom and a vertical wall b about 1.6 cm high; 2. from a lid c, also made of transparent glass and of the same outer diameter and the same wall thickness as the bowl a. The vertical wall d of the lid c can be chosen as high as desired; the amount depends on the amount of chemical substances required for absorption. In general, it will need to be about 1.2 cm high. The wall d has a horizontal bend, so far that the lid c can find a support on the side wall b of the shell a. After the bend, the wall d continues vertically downwards into the wall part e, makes a new horizontal bend and is then brought vertically upwards with part f up to about 2 mm to the cover surface. In this way, a groove g is formed inside the lid which runs around the lid.
An einer beliebigen Stelle am äußeren Rande der Deckelfläche, z. B. bei i, ist ein Loch von etwa 6 mm Durchmesser in das Glas der Deckelfläche gebohrt. Durch diese Öffnung i gelangt man unmittelbar in die Rinne g.At any point on the outer edge of the cover surface, e.g. B. at i, a hole about 6 mm in diameter is drilled into the glass of the lid surface. This opening i leads directly into the channel g.
Die Benutzung der Vorrichtung geschieht in folgender Weise: Deckel und Schale werden in bekannter Weise durch Hitze sterilisiert. Nach dem Abkühlen bringt man den durch Erwärmen verflüssigten Nährboden auf den Boden der Schale und impft ihn mit dem zu züchtenden Material. Dann füllt man durch die Öffnung i eine der beiden chemischen Komponenten, die zur Sauerstoffabsorption notwendig sind, z. B. Pyrogallussäure, in die Rinne g. Darauf fügt man, ebenfalls durch die Öffnung i, die zweite Komponente, z. B. Kalilauge, hinzu und verschließt nun den zwischen Schale a und Deckel c noch vorhandenen Spalt (e bis b) mit einer luftundurchlässigen knetbaren Masse, z. B. dem bekannten Plastulin, und in gleicher Weise die Öffnung i. Die alkalische Pyrogallussäurelösung nimmt dann den Sauerstoff der Luft, der zwischen Schale und Deckel eingeschlossen ist, auf, und die Keime, die auf oder in dem Nährboden enthalten sind, wachsen so unter Sauerstoffausschhlß.The device is used in the following way: the lid and bowl are heat-sterilized in a known manner. After cooling, the nutrient medium, liquefied by heating, is brought to the bottom of the dish and inoculated with the material to be grown. Then one of the two chemical components that are necessary for oxygen absorption is filled through the opening i, e.g. B. pyrogallic acid, in the gutter g. Then, also through the opening i, the second component, e.g. B. potassium hydroxide solution, and now closes the gap (e to b) still present between shell a and cover c with an air-impermeable kneadable mass, e.g. B. the known plastulin, and in the same way the opening i. The alkaline pyrogallic acid solution then absorbs the oxygen in the air, which is enclosed between the bowl and the lid, and the germs which are contained on or in the nutrient medium grow in this way with the exclusion of oxygen.
Die Vorrichtung unterscheidet sich von den bisher bekannten Apparaten dadurch, daß die sauerstoffabsorbierenden Substanzen in einer Rinne untergebracht sind, daß die Mischung dieser Stoffe erst zu erfolgen braucht, wenn Deckel und Schale schon luftdicht miteinander verschlossen sind, daß es nach der Mischung nur nötig ist, das Loch in der Deckelfläche zu verschließen.The device differs from the previously known apparatus in that the oxygen-absorbing substances are housed in a channel are that the mixture of these substances only needs to take place when the lid and bowl are already hermetically sealed with each other that it is only necessary after mixing is to close the hole in the lid surface.
Die Vorteile der Schale sind besonders darin zu sehen, daß sie als kreisrunde Doppelschale sehr handlich und wenig empfindlich ist, daß sie geringen Raum einnimmt, daß man die Schale auch senkrecht halten kann, wobei sich die sauerstoffabsorbierende Substanz im tiefsten Teil der Rinne ansammelt. Die ganze Fläche des Nährbodens kann bequem überschaut werden.The advantages of the shell are particularly to be seen in the fact that it is used as a circular double shell is very handy and not very sensitive that it is small Takes space that you can hold the shell vertically, with the oxygen-absorbing Substance accumulates in the deepest part of the gutter. The whole area of the culture medium can can be easily surveyed.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEZ16176D DE479304C (en) | Device for breeding microorganisms in the absence of oxygen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DEZ16176D DE479304C (en) | Device for breeding microorganisms in the absence of oxygen |
Publications (1)
Publication Number | Publication Date |
---|---|
DE479304C true DE479304C (en) | 1929-07-13 |
Family
ID=7623972
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DEZ16176D Expired DE479304C (en) | Device for breeding microorganisms in the absence of oxygen |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE479304C (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1206119B (en) * | 1962-05-22 | 1965-12-02 | Dr Heinrich Ott | Device for the cultivation of anaerobic and CO-loving microorganisms |
EP0811054A1 (en) * | 1994-11-10 | 1997-12-10 | BANES, Albert J. | Culture plate with splash guard |
WO2000017315A1 (en) * | 1998-09-24 | 2000-03-30 | Stefan Marotzki | Method and device for accommodating a cell culture |
WO2014127774A1 (en) * | 2013-02-21 | 2014-08-28 | Pharmamedia Dr. Mueller Gmbh | Container for accommodating nutrient media |
-
0
- DE DEZ16176D patent/DE479304C/en not_active Expired
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1206119B (en) * | 1962-05-22 | 1965-12-02 | Dr Heinrich Ott | Device for the cultivation of anaerobic and CO-loving microorganisms |
EP0811054A1 (en) * | 1994-11-10 | 1997-12-10 | BANES, Albert J. | Culture plate with splash guard |
EP0811054A4 (en) * | 1994-11-10 | 1999-04-07 | Albert J Banes | Culture plate with splash guard |
WO2000017315A1 (en) * | 1998-09-24 | 2000-03-30 | Stefan Marotzki | Method and device for accommodating a cell culture |
EP0999266A1 (en) * | 1998-09-24 | 2000-05-10 | Stefan Dr. Marotzki | Process and apparatus for taking up a cell culture |
WO2014127774A1 (en) * | 2013-02-21 | 2014-08-28 | Pharmamedia Dr. Mueller Gmbh | Container for accommodating nutrient media |
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