DE420725C - Method for measuring the germ content of the air - Google Patents

Method for measuring the germ content of the air

Info

Publication number
DE420725C
DE420725C DEL60476D DEL0060476D DE420725C DE 420725 C DE420725 C DE 420725C DE L60476 D DEL60476 D DE L60476D DE L0060476 D DEL0060476 D DE L0060476D DE 420725 C DE420725 C DE 420725C
Authority
DE
Germany
Prior art keywords
air
measuring
germs
nutrient
precipitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEL60476D
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LURGI APPBAU GmbH
Original Assignee
LURGI APPBAU GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LURGI APPBAU GmbH filed Critical LURGI APPBAU GmbH
Priority to DEL60476D priority Critical patent/DE420725C/en
Application granted granted Critical
Publication of DE420725C publication Critical patent/DE420725C/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F10/00—Homopolymers and copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

Verfahren zur Messung des Keinigehalts der Luft. Man hat zwecks Messung der in der Luft enthaltenen Schwebeteilchen eine bestimmte Luftmenge durch ein elektrisches Niederschlagsfeld hindurchgeleitet und die Schwebeteilchen auf einer Niederschlagsfläche, z. B. der Niederschlagselektrode, aufgefangen. Gemäß der Erfindung wird die in den Schwebeteilchen enthaltene Keim- und Bakterierunenge der Messung zugänglich gemacht, indem die Niederschlagsfläche mit einer Nährflüssigkei-oder Nährgelatine versehen wird, auf der man die Keime zur Entwicklung bringt. Handelt es sich z. B. um Schimmelpilze, die man auf die angegebene Weise niedergeschlagen hat, die aber an Zahl zu gering sind, um mit bloßem Auge festgestellt werden zu könnten, so erhält man nach ;einiger Zeit an der Oberfläche des Nährbodens, auf welchem der Niederschlag erfolgt ist, einen mit bloßem Auge-erkennbaren Schimmelansatz. Behandel-, man nach diesem Verfahren mehrere verschiedene Luftarten unter sonst gleichen Bedingungen -, so läßt sich der Grad der Keimfreiheit der einzelnen behandelten Luftmengen feststellen, denn diejenige Luftmenge, welche die kleinste Pilzkultur entstehen 'läßt, wird naturgemäß auch die wenigsten Keime enthalten haben. Bei bekanntem Wachstum der betreffenden Balkterienart und unter Zugrundelegung der bekannten Wachstumsbedingungen I Z> Z, läßt sich so ohne weiteres die absolute Bakterienmenge auf den Kubikmeter Luft finden.Procedure for measuring the absence of air. For the purpose of measuring the suspended particles contained in the air, a certain amount of air was passed through an electrical precipitation field and the suspended particles were deposited on a precipitation surface, e.g. B. the collecting electrode, collected. According to the invention, the amount of germs and bacteria contained in the suspended particles is made accessible for measurement by providing the precipitation surface with a nutrient liquid or nutrient gelatin on which the germs are brought to develop. Is it z. For example, molds that have been knocked down in the manner indicated, but which are too few in number to be seen with the naked eye, will be found after some time on the surface of the nutrient medium on which the precipitation occurred , a mold that is visible to the naked eye. If several different types of air are treated according to this method under otherwise identical conditions, then the degree of sterility of the individual treated air quantities can be determined, because the amount of air which gives rise to the smallest fungal culture will naturally also have contained the fewest germs. If the growth of the respective species of Balkteria is known and based on the known growth conditions I Z> Z, the absolute amount of bacteria per cubic meter of air can easily be found in this way.

Die Niederschlaigselektrode für das Verfahren läßt sich beliebig ausführen; sie kann z. B. mit einer Nährflüssigkeit, z. B. Bouillon o.dgl., befeuchtet werden, ab-er auch von vornherein aus einer geeigneten Nährmasse für Keime, z. B. Nährgelatine, hergestellt bzw. mit einer solchen NäUrmasse bedeckt sein.The precipitation electrode for the process can be designed in any way; she can z. B. with a nutrient liquid, e.g. B. bouillon or the like., Be moistened, from the outset from a suitable nutrient mass for germs, z. B. nutrient gelatin, be manufactured or covered with such a wet mass.

Natürlich empfiehlt es sich, der Niederschlagse'.#ektrode eine für die spätere mikroskopische Untersuchung geeignete Form zu geben.Of course, it is recommended that the precipitation '. # Ektrode one for the subsequent microscopic examination to give it a suitable shape.

Claims (1)

PATENT- ANS PRU C II: Verfahren zur Messung des Keimgehalts der Luft, dadurch gekennzeichnet, daß eine bestimmte Luftmenge zwecks Ausscheidung der Schwebe,eilchen in bekannter Weise durch ein elektrisches Niederschlagsfeld zwischen wenigstens zwei ge-,genpoligen Elektroden hindurchgel#ehet wird, und die NiederscIlilagsfläche, z. B. Niederschlagselektrode, für die in dieser Luftmenge befindlichen Schwebekörper sowie Bakterien, und Keime mit einer Nährflüssigkeit oder Nährgelatine versehen wird, auf welcher die aufgefajngenen Bakterien und Keime zur Entwicklung gelangen.PATENT- ANS PRU C II: Method for measuring the germ content of the air, characterized in that a certain amount of air for the purpose of excreting the suspended particles is in a known manner through an electrical precipitation field between at least two opposing, opposing polarity electrodes, and the Deposit area, e.g. B. Precipitation electrode, for the suspended bodies in this amount of air, as well as bacteria and germs, is provided with a nutrient liquid or nutrient gelatin on which the captured bacteria and germs develop.
DEL60476D 1924-06-20 1924-06-20 Method for measuring the germ content of the air Expired DE420725C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEL60476D DE420725C (en) 1924-06-20 1924-06-20 Method for measuring the germ content of the air

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEL60476D DE420725C (en) 1924-06-20 1924-06-20 Method for measuring the germ content of the air

Publications (1)

Publication Number Publication Date
DE420725C true DE420725C (en) 1925-10-30

Family

ID=7280119

Family Applications (1)

Application Number Title Priority Date Filing Date
DEL60476D Expired DE420725C (en) 1924-06-20 1924-06-20 Method for measuring the germ content of the air

Country Status (1)

Country Link
DE (1) DE420725C (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450850A2 (en) * 1990-03-27 1991-10-09 Kuraray Co., Ltd. Method and apparatus for determining air borne bacteria
WO2011050915A1 (en) 2009-10-29 2011-05-05 Rheinmetall Waffe Munition Gmbh Recoil catch in particular for a gas-operated weapon

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450850A2 (en) * 1990-03-27 1991-10-09 Kuraray Co., Ltd. Method and apparatus for determining air borne bacteria
EP0450850A3 (en) * 1990-03-27 1992-02-26 Kuraray Co., Ltd. Method and apparatus for determining air borne bacteria
WO2011050915A1 (en) 2009-10-29 2011-05-05 Rheinmetall Waffe Munition Gmbh Recoil catch in particular for a gas-operated weapon

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