DE4112258A1 - Metal-free HPLC column with good mechanical strength - comprises non-metallic e.g. polyether-ether]-ketone] etc. contg. metallic column, adaptor with inner- or outer- thread and metal-free filter-sieve-sandwich packet - Google Patents

Metal-free HPLC column with good mechanical strength - comprises non-metallic e.g. polyether-ether]-ketone] etc. contg. metallic column, adaptor with inner- or outer- thread and metal-free filter-sieve-sandwich packet

Info

Publication number
DE4112258A1
DE4112258A1 DE19914112258 DE4112258A DE4112258A1 DE 4112258 A1 DE4112258 A1 DE 4112258A1 DE 19914112258 DE19914112258 DE 19914112258 DE 4112258 A DE4112258 A DE 4112258A DE 4112258 A1 DE4112258 A1 DE 4112258A1
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DE
Germany
Prior art keywords
column
metal
metallic
free
sieve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19914112258
Other languages
German (de)
Inventor
Edgar Grom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE19914112258 priority Critical patent/DE4112258A1/en
Publication of DE4112258A1 publication Critical patent/DE4112258A1/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body

Abstract

The HPLC column comprises a non-metallic material e.g. PEEK, Teflon (RTM), KEL-F (RTM), Tefzel (RTM) and other non-metallic materials which are solvent resistant and have high metallic strength. The column end has a metallic column adapter (claimed). The column is situated in a metal column holder having an internal- or external thread. The column has a filter-sieve-sandwich-packet which is a combination of metal-free and glass fibre filter. USE/ADVANTAGE - The metal-free HPLC column is used in ion chromatography. The column can be screwed on as often as desired and with sufficient force without being rendered unusable. The filter-sieve-sandwich-package is not pressure sensitive (prone to shattering) and does not block easily when used for protein sepn

Description

HPLC-Säulen aus nichtmetallischem Werkstoff werden hauptsächlich in der Ionenchromatographie verwendet, um Konterminationen mit Metallionen zu vermeiden.HPLC columns made of non-metallic material are mainly used used in ion chromatography to terminate with To avoid metal ions.

Die z.Zt. verwendeten Säulen aus Peek-Material haben 2 gravie­ rende Mängel:The currently The columns made of peek material have 2 gravies deficiencies:

  • 1. Das Befestigungsgewinde für den Säulenkopf ist direkt auf die Peek-Säule geschnitten und wird bei zu starkem Anziehen des Kopfes (sonst Säule undicht) unbrauchbar.1. The mounting thread for the column head is cut directly onto the peek column and will if the head is tightened too much (otherwise pillar leaking) unusable.
  • 2. Die als Säulenabschluß verwendeten Keramikfritten sind druckempfindlich (zerspringen) und ver­ stopfen (z. B. bei Proteintrennungen) sehr schnell.2. The ceramic frits used as the end of the column are sensitive to pressure (shatter) and ver stuff very (e.g. with protein separations) fast.

Der Erfindung der metallfreien HPLC-Säule liegt die Aufgabe zugrunde, nicht metallische Säulen mit stabilem (metallischem) Befestigungsgewinde bzw. metallischem Säulenhalter sowie idealem Säu­ lenabschluß herzustellen.The invention of the metal-free HPLC column based on the task, not metallic pillars with stable (metallic) fastening thread or metallic column holder and ideal column to produce a final seal.

Die Aufgabe wird dadurch gelöst:This solves the task:

  • a) auf der nichtmetallischen Säule befindet sich ein Säulenadapter aus Metall, welcher sämtliche Schraubbewegungen erfährt. (Fig. 1)a) on the non-metallic column is a column adapter made of metal, which experiences all screwing movements. ( Fig. 1)
  • b) Die nichtmetallische Säule befindet sich in einem Säulenhalter aus Metall mit Innengewinde. (Fig. 2)b) The non-metallic column is located in a column holder made of metal with an internal thread. ( Fig. 2)
  • c) Die nichtmetallische Säule befindet sich in einem Säulenhalter aus Metall mit Außengewinde. (Fig. 3)c) The non-metallic column is located in a column holder made of metal with an external thread. ( Fig. 3)
  • d) Als Säulenabschluß wird keine Fritte, sondern ein Filter-Sieb- Sandwich-Paket eingesetzt, welches aus metallfreiem Gewebe sowie aus Glasfaserfilter besteht.d) A frit is not used as the end of the column, but a filter screen Sandwich package used, which is made of metal-free fabric and consists of glass fiber filter.

Der Vorteil der Erfindung besteht darin, daß die metallfreie HPLC-Säule beliebig oft und mit ausreichender Anpreßkraft ver­ schraubt werden kann. Außerdem ist ein Zerstören und Verstopfen der Säulenabdichtung nicht mehr möglich.The advantage of the invention is that the metal-free HPLC column as often as required and with sufficient contact pressure can be screwed. There is also destruction and clogging the column seal is no longer possible.

AusführungsbeispieleEmbodiments

Fig. 1 HPLC-Säule aus nichtmetallischem Werkstoff mit me­ tallischem Säulenadapter. Fig. 1 HPLC column made of non-metallic material with metallic column adapter.

Fig. 2 HPLC-Säule mit Säulenhalter aus Metall mit Innen­ gewinde. Fig. 2 HPLC column with column holder made of metal with internal thread.

Fig. 3 HPLC-Säule mit Säulenhalter aus Metall mit Außen­ gewinde. Fig. 3 HPLC column with column holder made of metal with an external thread.

ohne Bild. Filter-Sieb-Sandwich-Paket: Kombination von metallfreiem Gewebe und Glas­ faserfilter sowie PTFE-Halterung.without picture. Filter-sieve sandwich package: Combination of metal-free fabric and glass fiber filter and PTFE holder.

Claims (4)

1. HPLC-Säule aus nichtmetallischem Werkstoff (Peek, Teflon, Kel-F, Tefzel, sowie sämtliche nichtmetallische Werkstoffe, welche lösungsmittelbeständig sind und eine hohe mechanische Festigkeit vorweisen), dadurch gekennzeichnet, daß sich an den Säulenenden ein metallischer Säulenadapter befindet. (Fig. 1)1. HPLC column made of non-metallic material (Peek, Teflon, Kel-F, Tefzel, as well as all non-metallic materials that are solvent-resistant and have high mechanical strength), characterized in that there is a metallic column adapter at the column ends. ( Fig. 1) 2. HPLC-Säule aus nicht metallischem Werkstoff (Peek, Teflon, Kel-F, Tefzel, sowie sämtliche nichtmetallische Werkstoffe, welche lösungsmittelbeständig sind und eine hohe mechanische Festigkeit vorweisen), dadurch gekennzeichnet, daß sich die Säule in einem Säulenhalter aus Metall mit Innengewinde befindet. (Fig. 2)2. HPLC column made of non-metallic material (Peek, Teflon, Kel-F, Tefzel, and all non-metallic materials that are solvent-resistant and have a high mechanical strength), characterized in that the column in a metal column holder with an internal thread located. ( Fig. 2) 3. HPLC-Säule aus nicht metallischem Werkstoff (Peek, Teflon, Kel-F, Tefzel, sowie sämtliche nichtmetallische Werkstoffe, welche lösungsmittelbeständig sind und eine hohe mechanische Festigkeit vorweisen), dadurch gekennzeichnet, daß sich die Säule in einem Säulenhalter aus Metall mit Außengewinde befindet. (Fig. 3)3. HPLC column made of non-metallic material (Peek, Teflon, Kel-F, Tefzel, as well as all non-metallic materials that are solvent-resistant and have high mechanical strength), characterized in that the column in a metal column holder with an external thread located. ( Fig. 3) 4. Säulenabschluß als Filter-Sieb-Sandwich-Paket. (eine Kombination von metallfreiem Gewebe und Glasfaserfilter)4. Column end as filter-sieve sandwich package. (a combination of metal-free fabric and glass fiber filter)
DE19914112258 1991-04-15 1991-04-15 Metal-free HPLC column with good mechanical strength - comprises non-metallic e.g. polyether-ether]-ketone] etc. contg. metallic column, adaptor with inner- or outer- thread and metal-free filter-sieve-sandwich packet Withdrawn DE4112258A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19914112258 DE4112258A1 (en) 1991-04-15 1991-04-15 Metal-free HPLC column with good mechanical strength - comprises non-metallic e.g. polyether-ether]-ketone] etc. contg. metallic column, adaptor with inner- or outer- thread and metal-free filter-sieve-sandwich packet

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19914112258 DE4112258A1 (en) 1991-04-15 1991-04-15 Metal-free HPLC column with good mechanical strength - comprises non-metallic e.g. polyether-ether]-ketone] etc. contg. metallic column, adaptor with inner- or outer- thread and metal-free filter-sieve-sandwich packet

Publications (1)

Publication Number Publication Date
DE4112258A1 true DE4112258A1 (en) 1992-10-22

Family

ID=6429617

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19914112258 Withdrawn DE4112258A1 (en) 1991-04-15 1991-04-15 Metal-free HPLC column with good mechanical strength - comprises non-metallic e.g. polyether-ether]-ketone] etc. contg. metallic column, adaptor with inner- or outer- thread and metal-free filter-sieve-sandwich packet

Country Status (1)

Country Link
DE (1) DE4112258A1 (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE9405378U1 (en) * 1994-03-30 1994-06-23 Hewlett Packard Gmbh Separation column for chromatography
DE9413553U1 (en) * 1994-08-23 1994-10-13 Hewlett Packard Gmbh Connecting capillary
EP0624795A2 (en) 1993-05-14 1994-11-17 Upchurch Scientific, Inc. Column for liquid chromatography
DE4326568A1 (en) * 1993-08-07 1995-02-09 Edgar Grom Capillary HPLC column system
DE29512372U1 (en) * 1995-08-01 1995-10-05 Hewlett Packard Gmbh Separation column for chromatography
WO2002006818A1 (en) * 2000-07-13 2002-01-24 Merck Patent Gmbh Connecting system for plastic columns
WO2003005019A1 (en) * 2001-07-06 2003-01-16 Api Corporation Method of determining pyrithione metal salt
WO2004103518A1 (en) * 2003-05-14 2004-12-02 E.I. Dupont De Nemours And Company Protein separation column
WO2004104024A2 (en) * 2003-05-14 2004-12-02 E.I. Dupont De Nemours And Company Protein treatment process
DE102009001756A1 (en) 2009-03-23 2009-07-30 Agilent Technologies Inc., Santa Clara Filter screen e.g. dirt filter, for sample separation device e.g. high-performance liquid chromatography system, has filter arranged at inlet and/or outlet of separating column and impermeable for particles with size above threshold value

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0624795A2 (en) 1993-05-14 1994-11-17 Upchurch Scientific, Inc. Column for liquid chromatography
EP0624795A3 (en) * 1993-05-14 1994-12-21 Upchurch Scientific, Inc. Column for liquid chromatography
DE4326568A1 (en) * 1993-08-07 1995-02-09 Edgar Grom Capillary HPLC column system
DE9405378U1 (en) * 1994-03-30 1994-06-23 Hewlett Packard Gmbh Separation column for chromatography
US5651886A (en) * 1994-03-30 1997-07-29 Hewlett-Packard Company Separation column for chromatography
US5614154A (en) * 1994-08-23 1997-03-25 Hewlett-Packard Company Connecting capillary
DE9413553U1 (en) * 1994-08-23 1994-10-13 Hewlett Packard Gmbh Connecting capillary
DE29512372U1 (en) * 1995-08-01 1995-10-05 Hewlett Packard Gmbh Separation column for chromatography
WO2002006818A1 (en) * 2000-07-13 2002-01-24 Merck Patent Gmbh Connecting system for plastic columns
US6797174B2 (en) * 2000-07-13 2004-09-28 Merck Patent Gmbh Connecting system for plastic columns
WO2003005019A1 (en) * 2001-07-06 2003-01-16 Api Corporation Method of determining pyrithione metal salt
WO2004103518A1 (en) * 2003-05-14 2004-12-02 E.I. Dupont De Nemours And Company Protein separation column
WO2004104024A2 (en) * 2003-05-14 2004-12-02 E.I. Dupont De Nemours And Company Protein treatment process
WO2004104024A3 (en) * 2003-05-14 2005-03-17 Du Pont Protein treatment process
DE102009001756A1 (en) 2009-03-23 2009-07-30 Agilent Technologies Inc., Santa Clara Filter screen e.g. dirt filter, for sample separation device e.g. high-performance liquid chromatography system, has filter arranged at inlet and/or outlet of separating column and impermeable for particles with size above threshold value

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