DE4012351A1 - Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax - Google Patents

Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax

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Publication number
DE4012351A1
DE4012351A1 DE4012351A DE4012351A DE4012351A1 DE 4012351 A1 DE4012351 A1 DE 4012351A1 DE 4012351 A DE4012351 A DE 4012351A DE 4012351 A DE4012351 A DE 4012351A DE 4012351 A1 DE4012351 A1 DE 4012351A1
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flax
prepn
obtd
esterase
enzymes
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Inventor
Mariana Lalova Petrova
Nikolina Mancheva Yanakieva
Simeon Grigorievitch Tchaga
Dobri Botev Tupachki
Larisa Yilievna Geleva
Albena Petrova Anastassova
Zdravko Todorov Zanov
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Priority to DE4012351A priority Critical patent/DE4012351A1/en
Priority to FR9005422A priority patent/FR2661420A1/en
Priority to CN90102756A priority patent/CN1056529A/en
Publication of DE4012351A1 publication Critical patent/DE4012351A1/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00—Treatment of vegetable material
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/685—Aspergillus niger

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Textile Engineering (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Paper (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

A macerating enzyme prepn. obtd. with the aid of Aspergillus niger 76-1 (National Bank for industrial organisms and cell cultures in Sofia, Bulgaria, registration no. 1333) contains the following activities: polygalactouronase, pectinoesterase, protopectinase and certain amts. of protease, beta-gluconase, glucoamylase and cellulase. USE/ADVANTAGE - The enzyme prepn. macerates bast fibres and can be used in the textile industry in the primary work up of flax. The enzymes accelerate the degradation of the protopectins, the soluble pectins and hemicellulose without affecting the inner structure or mechanical strength of the fibre. The enzymes facilitate the prepn. of flax fibres with much fuller utilisation of the raw flax maerial, improve mechanisation and control of the technological processes and shorten the processes. No significant changes to equipment need be made.

Description

Die Erfindung betrifft ein Enzympräparat, das Bast-Faser­ pflanzen mazerisiert und in der Textilindustrie bei der primären Bearbeitung von Flachs Verwendung finden kann.The invention relates to an enzyme preparation, the bast fiber plants macerated and in the textile industry at primary processing of flax can be used.

Es ist ein mazerisiertes Enzympräparat bekannt (Urheber­ schein SU 6 22 838), das mit Hilfe von Ciostridium Felsineum 22 und Ciostridium pectinofermenans 1 erhalten wird.A macerated enzyme preparation is known Schein SU 6 22 838) with the help of Ciostridium Felsineum 22 and Ciostridium pectinofermenans 1 is obtained.

Der Nachteil dieses Präparats ist darin zu sehen, daß es eine schwache hydrolytische und liasische Wirkung sowie eine niedrige Pektin-Eliminase Aktivität (6,6 Einheiten/ml) aufweist.The disadvantage of this preparation is that it a weak hydrolytic and liasic effect as well low pectin eliminase activity (6.6 units / ml) having.

Es ist auch ein weiteres mazerisierendes Enzympräparat bekannt (Urheberschein SU 10 17 728), das mit Hilfe von Bacillus circulans 31 und Bacillus sp. erhalten worden ist, welches Endu-Polygalakturonase, Exo-Polygalakturonase und Pektin-Trans-Eliminase enthält.It is also another macerating enzyme preparation known (copyright certificate SU 10 17 728), which with the help of Bacillus circulans 31 and Bacillus sp. has been obtained which endu-polygalacturonase, exo-polygalacturonase and Contains pectin trans eliminase.

Ein Nachteil dieses Präparats ist seine ungenügende mazeri­ sierende Aktivität.A disadvantage of this preparation is its insufficient maceration activity.

Es ist auch ein mazerisierendes Enzympräparat bekannt (Urheberschein SU 8 34 271), das mit Hilfe von Penicillium digitatum 24 erhalten wurde, welches Endo-Polygalakturona­ se, Exo-Polygalakturonase und Trans-Eliminase enthält.A macerating enzyme preparation is also known (Copyright certificate SU 8 34 271) with the help of Penicillium  digitatum 24 was obtained, which endo-polygalacturona se, exo-polygalacturonase and trans-eliminase.

Es ist ferner ein Verfahren zur Bearbeitung von Flachs mit pektolytischen Enzymen bekannt, die durch Penicillium digitatum 24P und zusätzlich mit Enzymen, erhalten mit Hilfe von Aspergillus carbonarius 77, synthetisiert wurden und eine zellulytische und hämizellulytische Wirkung zeigen (Urheberschein SU 8 34 271).It is also a method of processing flax with pectolytic enzymes known by Penicillium digitatum 24P and additionally with enzymes, obtained with Help from Aspergillus carbonarius 77, were synthesized and show cellulytic and hemicellulytic activity (Copyright certificate SU 8 34 271).

Nachteile des bekannten Präparats sind die niedrige Aktivi­ tät der einzelnen Enzyme, die angewandte Verarbeitung von ganzen Flachsstengeln, was mit hohem Verlust an Flachsroh­ stoff verbunden ist, die lange Verfahrensdauer, der hohe Verbrauch an Wasser und Elektroenergie und die verschlech­ terten Exploitationsbedingungen. Es werden lange und kurze Flachsfasern erhalten, wobei vorwiegend die langen Fasern verwendet werden.Disadvantages of the known preparation are the low levels of activity of the individual enzymes, the processing of whole flax stalks, what with high loss of flax raw is connected, the long process time, the high Consumption of water and electrical energy and the worsening exploitation conditions. It will be long and short Preserved flax fibers, predominantly the long fibers be used.

Der Erfindung liegt die Aufgabe zugrunde ein mazerisieren­ des Enzympräparat zu entwickeln, das eine Kombination aus Enzymen zum beschleunigten Abbau des Protopektins, der löslichen Pektinstoffe und Hämizellulose enthält, ohne daß dabei die innere Struktur und mechanische Festigkeit der Faser beeinträchtigt wird, sowie ein Verfahren zur Herstellung von Flachsfasern zu entwickeln, das eine viel vollere Ausnutzung des Flachsrohstoffs erlaubt, eine Mecha­ nisierung und Kontrolle der technologischen Prozesse, Verbesserung der Exploitationsbedingungen in den Betrieben zur primären Verarbeitung des Flachses vorsieht, sowie eine wesentliche Verkürzung des Prozesses ergibt.The invention has for its object a macerize to develop the enzyme preparation, which is a combination of Enzymes for the accelerated degradation of the protopectin, the contains soluble pectin substances and hemicellulose without thereby the internal structure and mechanical strength of the Fiber is affected, as well as a process for Developing flax fiber to develop a lot full utilization of the flat raw material allows a mecha nization and control of technological processes, Improvement of exploitation conditions in companies for primary processing of the flax, and a significantly shortens the process.

Diese Aufgabe wird durch ein Enzympräparat, produziert vom Stamm Aspergillus niger 76-1 und registriert in der Natio­ nalen Bank für industrielle Mikroorganismen und Zellkulturen in Sofia, Bulgarien unter der Nummer 1333, gelöst.This task is carried out by an enzyme preparation produced by Strain Aspergillus niger 76-1 and registered in the Natio  nal bank for industrial microorganisms and Cell cultures in Sofia, Bulgaria under number 1333, solved.

Das Fermentationsmedium enthält Abfallprodukte aus der Nahrungsmittelindustrie als Quelle von Kohlenstoff, Stick­ stoff und anderen Wachstumfaktoren sowie Mineralsalze, welche die anderen Elemente liefern. Bei Tiefkultivierung des Stamms werden in der Fermentationsflüssigkeit 1000- 12000 E/cm3 Polygalakturonase, 15-20 E/cm3 Pektinesterase, 1-2 E/cm3 Protopektinase akkumuliert und als begleitende Enzyme saure Protease, β-Glukanase und Glukoamylase, welche die mazerisierende Wirkung verstärken. Der so erhaltene Komplex aus Enzymen wird zum biochemischen Abbau der Binde­ mittel im Flachs eingesetzt.The fermentation medium contains waste products from the food industry as a source of carbon, nitrogen and other growth factors as well as mineral salts, which provide the other elements. When the strain is deeply cultivated, 1000-12000 U / cm 3 polygalacturonase, 15-20 U / cm 3 pectin esterase, 1-2 U / cm 3 protopectinase are accumulated in the fermentation liquid and acidic protease, β-glucanase and glucoamylase, which are the accompanying enzymes Increase macerating effect. The complex of enzymes thus obtained is used for the biochemical degradation of the binding agent in flax.

Das Wesen des Verfahrens zur Herstellung von Flachsfasern durch Verwendung eines mazerisierenden Mittels besteht darin, daß man die Flachsstengel mechanisch bearbeitet, dann in eine Kammer einbringt und mit warmem Wasser über­ gießt. Das Enzympräparat, erhalten aus Asp. niger 76-1, wird in einer Menge von 0,5 bis 2% bezogen auf das Gewicht der Fasermasse zudosiert. Das Verfahren dauert 3 bis 4 Stunden unter kontinuierlicher Zirkulation der Flüssigkeit. Nach Beendigung der Mazeration wird die Abfallflüssigkeit mehrmals benutzt (4-5 mal) unter Zusatz einer minimalen Menge an Enzymen zur Kompensation der Verluste an Behand­ lungsflüssigkeit, die mit der Fasermasse ausgetragen worden ist. Die mit Enzymen bearbeiteten Flachsfasern werden getrocknet, auf Aufbereitungsmaschinen für kurze Flachsfa­ sern gehechelt und dann auf eine Flachskarde hingezogen.The essence of the process for producing flax fibers by using a macerating agent by mechanically working the flax stems, then placed in a chamber and covered with warm water pours. The enzyme preparation obtained from Asp. Niger 76-1, is in an amount of 0.5 to 2% by weight added to the fiber mass. The process takes 3 to 4 Hours with continuous circulation of the liquid. After the maceration has ended, the waste liquid used several times (4-5 times) with the addition of a minimal Amount of enzymes to compensate for the loss of treatment liquid that has been discharged with the fiber mass is. The flax fibers processed with enzymes are dried, on processing machines for short flax fa eaten and then dragged onto a flat card.

Dieses Verfahren kann in jedem Betrieb zur primären Verar­ beitung von Flachs eingesetzt werden, ohne eine wesentliche Änderung in der Ausrüstung zu erfordern.This procedure can be the primary processing in every company processing of flax can be used without an essential  Require change in equipment.

Die Vorzüge des erfindungsgemäßen Präparats und des Verfah­ rens sind wie folgt: Es wird ein Stamm, der auf einem billigen Nahrungsmittelme­ dium einen Komplex mit harmonischer Kombination von pekto­ lytischen, proteolytischen cellulolytischen Enzymen synthe­ siert, verwendet, der zum Extrahieren von Flachsfasern geeignet ist. Das Präparat wird in Form eines Konzentrats oder trockenen Produkts zur Mazeration von Flachs verwendet. Es wird zudem eine bessere Ausnutzung des Flachsrohstoffs erzielt. Es wird weiter eine Mechanisierung der Arbeit in der Landwirtschaft und in den Betrieben zur primären Verarbeitung des Flachs sowie eine wesentliche Abkürzung der Bearbeitung, eine Vergrößerung der Betriebs­ kapazität erzielt und nicht zuletzt wird der widrige Geruch beseitigt.The advantages of the preparation according to the invention and the process rens are as follows: It becomes a tribe based on a cheap food menu dium a complex with a harmonious combination of pekto lytic, proteolytic cellulolytic enzymes synthe siert, used for extracting flax fibers suitable is. The preparation is in the form of a concentrate or dry product for maceration of flax used. It will also make better use of the Flax raw material achieved. Mechanization continues work in agriculture and on farms primary processing of the flax as well as an essential Abbreviation of processing, an increase in operating capacity achieved and last but not least the bad smell eliminated.

Beispiel 1example 1

Aus einer 20tägigen Kultur von Asp. niger 76-1, entwickelt auf einem geneigten Malzagar wird die sporische Suspension vorbereitet und in einer Menge von 5.104 bis 1.105 Sporen/cm3 zum Einsetzen in das Fermentations-Nahrungsmittelmedium verwendet. Nach Beendigung der Fermentation werden pro 1 cm3 Flüssigkeit 12 000 ± 300 E von Polygalakturonase, 15 ± 5 E von Pektinesterase und 1 bis 1,5 E von Protopektinase akkumuliert. Nach Beseitigung der Miszellen und der nicht­ löslichen Komponenten des Mediums durch Filtrieren und Zentrifugieren wird die native Lösung gereinigt und danach ungefähr 10mal mittels Ultramembranenfiltration konzen­ triert. Es wird ein komplexes Enzympräparat, das 80 000 bis 100 000 E/cm3 Polygalakturonase, 160 bis 180 E/cm3 Pektines­ tearase, 10 bis 12 E/cm3 und gewisse Mengen begleitender Enzyme, wie saure Protease, β-Glukanase, Glukoamylase, Zellulase enthält, erhalten, wobei die begleitenden Enzyme die mazerisierenden Eigenschaften des Präparats verstärken. Das erhaltene Konzentrat kann getrocknet werden, wobei die Aktivität in den Grenzen 500 000 bis 700 000 E/g Polygal­ akturonase, 500 bis 700 E/g Pektinesterase und 70 bis 120 E/g Protopektinase liegt.The sporic suspension is prepared from a 20-day culture of Asp. Niger 76-1, developed on an inclined malt agar, and used in an amount of 5.10 4 to 1.10 5 spores / cm 3 for insertion into the fermentation food medium. After completion of the fermentation, 12,000 ± 300 U of polygalacturonase, 15 ± 5 U of pectin esterase and 1 to 1.5 U of protopectinase are accumulated per 1 cm 3 of liquid. After removal of the mis cells and the insoluble components of the medium by filtering and centrifuging, the native solution is cleaned and then concentrated approximately 10 times by means of ultramembrane filtration. It becomes a complex enzyme preparation, the 80,000 to 100,000 U / cm 3 polygalacturonase, 160 to 180 U / cm 3 pectin tearase, 10 to 12 U / cm 3 and certain amounts of accompanying enzymes, such as acidic protease, β-glucanase, glucoamylase , Contains cellulase, the accompanying enzymes enhancing the macerating properties of the preparation. The concentrate obtained can be dried, the activity being within the limits of 500,000 to 700,000 U / g polygal acturonase, 500 to 700 U / g pectin esterase and 70 to 120 U / g protopectinase.

Die Quantität des Enzympräparats gegenüber der trockenen Substanz des Flachs wird in Abhängigkeit der Polygalakturo­ nase-Aktivität von 1 g Flachsrohstoff dosiert, d. h. von 1000 bis 5000 E Polygalakturonase. Die Flachsstengel werden in einer Enthülsungsmaschine verarbeitet. Die erhaltene gleichmäßige Fasermasse, die 10 bis 13% mechanische Bei­ mengen enthält, wird mit warmem Wasser übergossen und nach Einstellen der Temperatur auf 40°C wird das Enzympräparat zudosiert. Die Mazeration erfolgt unter kontinuierlicher Zirkulation der Flüssigkeit während 3 bis 5 Stunden in Abhängigkeit von der eingelegten Enzymmenge und der Qualität des Flachsrohstoffs. Die verarbeitete Fasermasse wird 3mal mit warmem Wasser gewaschen (Temperatur 60°C). Man trocknet sie, hechelt sie und danach wird sie noch hingezogen, wobei man feine Flachsfasern mit Gehalt an Beimengen unter 1% erhält.The quantity of the enzyme preparation compared to the dry one The substance of flax becomes dependent on the polygalacturo dosed nose activity of 1 g of flax raw material, d. H. from 1000 to 5000 U polygalacturonase. The flax stems are processed in a unveiling machine. The received uniform fiber mass, the 10 to 13% mechanical contains quantities, is poured with warm water and after Setting the temperature to 40 ° C is the enzyme preparation added. The maceration takes place continuously Circulation of the liquid in 3 to 5 hours Depending on the amount of enzyme inserted and the Quality of the flat raw material. The processed fiber mass is washed 3 times with warm water (temperature 60 ° C). You dry it, pant it and then it becomes attracted, being fine flax fibers containing Admixtures below 1%.

Claims (2)

1. Mazerisierendes Enzympräparat, das mit Hilfe von Asp. niger 76-1, hinterlegt bei der Nationalen Bank für indu­ strielle Mikroorganismen und Zellkulturen in Sofia, Bulga­ rien, unter der Registriernummer 1333, erhalten worden ist, das folgende Aktivitäten aufweist: Polygalakturo­ nase-, Pektinoesterase-, Protopektinase- und gewisse Mengen Protease-, β-Glukonase-, Glukoamylase- und Zellulase- Aktivität.1. Macerating enzyme preparation, which with the help of Asp. niger 76-1, deposited with the National Bank for indu Strial microorganisms and cell cultures in Sofia, Bulga rien, under registration number 1333, which has the following activities: Polygalakturo nose, pectino esterase, protopectinase and certain amounts Protease, β-gluconase, glucoamylase and cellulase Activity. 2. Verfahren zur enzymatischen Bearbeitung von Flachsfa­ sern, dadurch gekennzeichnet, daß man die enthülsten Flachsstengel mit einem mazerisierenden Enzym gemäß Anspruch 1 feindosiert in Mengen von 0,5 bis 2% in bezug auf die Rohstoffmasse bei einer Temperatur von 38 bis 43°C während 3 bis 5 Stunden unter kontinuierli­ cher Zirkulation der Flüssigkeit behandelt.2. Process for the enzymatic processing of flax fa sern, characterized in that the exposed flax stalks with a macerating Enzyme according to claim 1 finely dosed in amounts of 0.5 to 2% in relation to the raw material mass at one temperature from 38 to 43 ° C for 3 to 5 hours under continuous Circulation of the fluid treated.
DE4012351A 1990-04-18 1990-04-18 Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax Withdrawn DE4012351A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE4012351A DE4012351A1 (en) 1990-04-18 1990-04-18 Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax
FR9005422A FR2661420A1 (en) 1990-04-18 1990-04-27 Enzymatic product of maceration and process for the treatment of linseed using this product
CN90102756A CN1056529A (en) 1990-04-18 1990-05-14 The working method of macerating enzymic product and flax

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4012351A DE4012351A1 (en) 1990-04-18 1990-04-18 Macerating enzyme prepn. obtd. from Aspergillus niger - contains polygalacturonase, pectin-esterase and proto-pectinase, which are used in the work up of flax
CN90102756A CN1056529A (en) 1990-04-18 1990-05-14 The working method of macerating enzymic product and flax

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DE (1) DE4012351A1 (en)
FR (1) FR2661420A1 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2713671A1 (en) * 1993-12-15 1995-06-16 Sofilin Sa Enzymatic retting process.
WO1996013632A1 (en) * 1994-10-28 1996-05-09 Novo Nordisk A/S A process for chemical finishing of insoluble polymers
WO1998045393A2 (en) * 1997-04-09 1998-10-15 Kao Corporation Detergent composition
WO2000056867A1 (en) * 1999-03-22 2000-09-28 Novozymes A/S An enzymatic treatment method
DE19854324B4 (en) * 1998-09-09 2005-01-20 Herres-Costard, Cornelia Process for the biological recovery of cellulose fiber material from calcium pectinate-containing, vegetable substrate
JPWO2016017814A1 (en) * 2014-07-31 2017-06-29 エイベックス・グループ・ホールディングス株式会社 Hemp fiber for spinning and hemp fiber for spinning
EP3176317A4 (en) * 2014-07-31 2018-02-28 Avex Group Holdings Inc. Method for manufacturing linen fiber for spinning, and linen fiber for spinning

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2699192B1 (en) * 1992-12-11 1995-05-12 Louis Hurdequint Enzymatic retting process controlling bast fibers and enzymatic retting solution.
CN101550606B (en) * 2008-04-01 2011-10-26 湖南逐鹿苎麻纺织有限公司 Ramie degumming method utilizing a complex enzyme preparation
CN115029795B (en) * 2022-07-14 2024-01-26 杭州惠邦纺织有限公司 Ramie fiber moisture-absorbing quick-drying fabric and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
- American Type Culture Collection, CATALOGUE OF FUNGI/YEASTS, Seventeenth Edition, 1987, S.438 *
DSM - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Catalogue of Strains 1989, Fourth Edition, S.259 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995016808A1 (en) * 1993-12-15 1995-06-22 Sofilin S.A. Enzymatic retting method
FR2713671A1 (en) * 1993-12-15 1995-06-16 Sofilin Sa Enzymatic retting process.
WO1996013632A1 (en) * 1994-10-28 1996-05-09 Novo Nordisk A/S A process for chemical finishing of insoluble polymers
US5733750A (en) * 1994-10-28 1998-03-31 Novo Nordisk A/S Process for chemical finishing of insoluble polymers
US6172030B1 (en) 1997-04-09 2001-01-09 Kao Corporation Detergent composition
WO1998045393A2 (en) * 1997-04-09 1998-10-15 Kao Corporation Detergent composition
WO1998045393A3 (en) * 1997-04-09 1999-05-27 Kao Corp Detergent composition
DE19854324B4 (en) * 1998-09-09 2005-01-20 Herres-Costard, Cornelia Process for the biological recovery of cellulose fiber material from calcium pectinate-containing, vegetable substrate
US6296671B1 (en) 1999-03-22 2001-10-02 Novorymeo A/S Enzymatic treatment method
WO2000056867A1 (en) * 1999-03-22 2000-09-28 Novozymes A/S An enzymatic treatment method
JPWO2016017814A1 (en) * 2014-07-31 2017-06-29 エイベックス・グループ・ホールディングス株式会社 Hemp fiber for spinning and hemp fiber for spinning
EP3176317A4 (en) * 2014-07-31 2018-02-28 Avex Group Holdings Inc. Method for manufacturing linen fiber for spinning, and linen fiber for spinning
EP3176318A4 (en) * 2014-07-31 2018-02-28 Avex Group Holdings Inc. Method for producing hemp fiber for spinning, and hemp fiber for spinning

Also Published As

Publication number Publication date
CN1056529A (en) 1991-11-27
FR2661420A1 (en) 1991-10-31

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