DE384635C - Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis - Google Patents

Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis

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Publication number
DE384635C
DE384635C DES56079D DES0056079D DE384635C DE 384635 C DE384635 C DE 384635C DE S56079 D DES56079 D DE S56079D DE S0056079 D DES0056079 D DE S0056079D DE 384635 C DE384635 C DE 384635C
Authority
DE
Germany
Prior art keywords
bacilli
tuberculosis
preparation
tubercle bacilli
vaccines
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DES56079D
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HUGO SELTER DR
Original Assignee
HUGO SELTER DR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HUGO SELTER DR filed Critical HUGO SELTER DR
Priority to DES56079D priority Critical patent/DE384635C/en
Application granted granted Critical
Publication of DE384635C publication Critical patent/DE384635C/en
Expired legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Communicable Diseases (AREA)
  • Pulmonology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Description

Verfahren zur Herstellung von Impfstoffen aus menschlichen Tuberkelbazillen oder Rindertuberkelbazillen zur Schutzimpfung gegen Tuberkulose. Die Erfindung betrifft ein Verfahren zur Herstellung von Impfstoffen aus menschlichen oder Rindertuberkelbazillen, bei denen lebende Tuberkelbazillen so lange verrieben werden, bis ein Präparat entsteht, das lebend aufgeschlossenes Bakterienprotoplasma in Verbindung mit einer geringen Zahl lebender Tuberkelbazillen enthält. Die bisher bekannten Verfahren zur Herstellung von Präparaten aus Tuberkelbazillen gingen von dem Gedanken aus, entweder lebende, völlig avirulente und andersartige Bazillen zu verwenden (z. B. zur Schutzimpfung von Rindern menschliche Tuberkelbazillen), oder Präparate aus zerriebenen Bazillen zu gewinnen, wobei die Zerreibung so weit getrieben wurde, daß in dem Endprodukt kein lebender Bazillus mehr nachweisbar war. Um ganz sicher zu gehen, wurden sogar diesen letzteren Präparaten noch besondere Sterilisationsmittel (Carbolsäure) zugesetzt. Im Gegensatz hierzu werden bei meiner Erfindüng die lebenden Tuberkelbazillen nur so weit verrieben, daß im Endprodukt stets noch eine gewisse Anzahl von ihnen im lebenden Zustand enthalten sind. Das neue Verfahren wird in der Weise ausgeübt, daß im. Achatmörser mit der Hand die Tuberkelbazillenmasse verrieben wird, wobei durch mikroskopische Untersuchung der verriebenen Masse von Zeit zu Zeit geprüft werden muß, ob und inwieweit das Endziel erreicht ist. Der Zeitpunkt des Rufhörens -richtet sich nach den zur Verwendung kommenden Kulturen. . Durch Untersuchungen (s. S e 1 t e r »Die Immunitätsverhältnisse bei Meerschweinchentuberkulose«, Zeitschr. f. Hygiene, Bd.95, S. 159 und »über die Wirkung abgetöteter Tuberkelbazillen«, Zeitschrift f. Hygiene, Bd. 95, S. 233) wurde bewiesen, daß die beobachtete Wirkung nur eintritt, wenn das lebend aufgeschlossene Protoplasma in Verbindung mit einer bestimmten Anzahl lebender Tuberkelbazillen zur Anwendung kommt, da weder das Protoplasma noch die in dem Präparat vorhandene Anzahl lebender Bazillen für sich allein den gewünschten Erfolg hervorbringen. Zur Erfindung gehört weiter, daß zur Schutzimpfung nur arteigene Tuberkelbazillen mit genügender Virulenz benutzt werden, so zur Schutzimpfung der Rinder Rindertuberkelbazillen, zur Schutzimpfung der Menschen menschliche Tuberkelbazillen.Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis. The invention relates to a method for the production of vaccines from human or bovine tubercle bacilli, in which live tubercle bacilli are ground until a preparation is obtained which contains living disrupted bacterial protoplasm in connection with a small number of live tubercle bacilli. The previously known processes for the production of preparations from tubercle bacilli were based on the idea of either using live, completely avirulent and different types of bacilli (e.g. human tubercle bacilli for the vaccination of cattle), or to obtain preparations from ground bacilli by trituration so far that no living bacillus was detectable in the end product. To be on the safe side, special sterilizing agents (carbolic acid) were even added to these latter preparations. In contrast to this, in my invention the living tubercle bacilli are only ground so far that the end product always contains a certain number of them in the living state. The new procedure is carried out in such a way that im. In an agate mortar, the mass of tubercle bacilli is rubbed by hand, whereby a microscopic examination of the rubbed mass must be checked from time to time to see whether and to what extent the end goal has been achieved. The time at which the call is heard depends on the cultures to be used. . Through investigations (see page 1 ter "The conditions of immunity in guinea pig tuberculosis", Zeitschr. F. Hygiene, vol. 95, p. 159 and "on the effects of killed tubercle bacilli", Zeitschrift f. Hygiene, vol. 95, p. 233 ) it has been proven that the observed effect only occurs when the live, disrupted protoplasm is used in conjunction with a certain number of live tubercle bacilli, since neither the protoplasm nor the number of living bacilli present in the preparation alone produce the desired result. The invention also includes the fact that only native tubercle bacilli with sufficient virulence are used for the vaccination, for example bovine tubercle bacilli for the vaccination of cattle and human tubercle bacilli for the vaccination of humans.

Claims (1)

PATENTANSPRUCH: Verfahren zur Herstellung von Impfstoffen aus menschlichen Tuberkelbazillen oder Rindertuberkelbazillen insbesondere zur Schutzimpfung von nicht angesteckten Rindern und Menschen gegen die Tuberkuloseansteckimg, dadurch gekennzeichnet, daß durch Verreiben lebender Kulturen ein Präparat gewonnen wird, welches das lebend aufgeschlossene Bakterienprotoplasma in Verbindung mit einer geringen Anzahl lebender Tuberkelbazillen enthält und ohne - Sterilisierung -verwendet wird. PATENT CLAIM: A process for the production of vaccines from human tubercle bacilli or bovine tubercle bacilli, in particular for the vaccination of uninfected cattle and humans against tuberculosis infection, characterized in that a preparation is obtained by triturating living cultures which contains the bacterial protoplasm that has been broken down in connection with a small number of living ones Contains tubercle bacilli and is used without sterilization.
DES56079D 1921-04-07 1921-04-07 Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis Expired DE384635C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DES56079D DE384635C (en) 1921-04-07 1921-04-07 Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DES56079D DE384635C (en) 1921-04-07 1921-04-07 Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis

Publications (1)

Publication Number Publication Date
DE384635C true DE384635C (en) 1923-11-03

Family

ID=7490858

Family Applications (1)

Application Number Title Priority Date Filing Date
DES56079D Expired DE384635C (en) 1921-04-07 1921-04-07 Process for the preparation of vaccines from human tuberculosis bacilli or bovine tubercle bacilli for protection against tuberculosis

Country Status (1)

Country Link
DE (1) DE384635C (en)

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