DE3734889A1 - Verfahren zur bestimmung der funktionsspiegel von protein c in menschlichem plasma und verwandter reagenzien - Google Patents
Verfahren zur bestimmung der funktionsspiegel von protein c in menschlichem plasma und verwandter reagenzienInfo
- Publication number
- DE3734889A1 DE3734889A1 DE19873734889 DE3734889A DE3734889A1 DE 3734889 A1 DE3734889 A1 DE 3734889A1 DE 19873734889 DE19873734889 DE 19873734889 DE 3734889 A DE3734889 A DE 3734889A DE 3734889 A1 DE3734889 A1 DE 3734889A1
- Authority
- DE
- Germany
- Prior art keywords
- activator
- test
- protein
- plasma
- beads
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 101800004937 Protein C Proteins 0.000 title claims abstract description 42
- 101800001700 Saposin-D Proteins 0.000 title claims abstract description 42
- 229960000856 protein c Drugs 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 34
- 102100036546 Salivary acidic proline-rich phosphoprotein 1/2 Human genes 0.000 title claims abstract 5
- 239000003153 chemical reaction reagent Substances 0.000 title description 5
- 239000012190 activator Substances 0.000 claims abstract description 24
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- 108010094028 Prothrombin Proteins 0.000 claims abstract description 9
- 102100027378 Prothrombin Human genes 0.000 claims abstract description 9
- 229940039716 prothrombin Drugs 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims description 22
- 239000011324 bead Substances 0.000 claims description 12
- 230000015271 coagulation Effects 0.000 claims description 12
- 238000005345 coagulation Methods 0.000 claims description 12
- 230000004913 activation Effects 0.000 claims description 11
- 239000002574 poison Substances 0.000 claims description 8
- 231100000614 poison Toxicity 0.000 claims description 8
- 241000271510 Agkistrodon contortrix Species 0.000 claims description 6
- 238000011990 functional testing Methods 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- 238000004737 colorimetric analysis Methods 0.000 claims description 3
- 102000017975 Protein C Human genes 0.000 description 37
- 108090000190 Thrombin Proteins 0.000 description 10
- 229960004072 thrombin Drugs 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000011049 pearl Substances 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 108010079274 Thrombomodulin Proteins 0.000 description 4
- 102100026966 Thrombomodulin Human genes 0.000 description 4
- 208000007536 Thrombosis Diseases 0.000 description 4
- 230000003024 amidolytic effect Effects 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- PGOHTUIFYSHAQG-LJSDBVFPSA-N (2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-sulfanylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-hydroxybutanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoic acid Chemical compound CSCC[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O PGOHTUIFYSHAQG-LJSDBVFPSA-N 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 108010000499 Thromboplastin Proteins 0.000 description 3
- 102000002262 Thromboplastin Human genes 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 102000004411 Antithrombin III Human genes 0.000 description 2
- 108090000935 Antithrombin III Proteins 0.000 description 2
- 108010080865 Factor XII Proteins 0.000 description 2
- 102000000429 Factor XII Human genes 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 101000595198 Homo sapiens Podocalyxin Proteins 0.000 description 2
- 239000008118 PEG 6000 Substances 0.000 description 2
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 2
- 102000007327 Protamines Human genes 0.000 description 2
- 108010007568 Protamines Proteins 0.000 description 2
- 229960005348 antithrombin iii Drugs 0.000 description 2
- 229940006612 barium citrate Drugs 0.000 description 2
- PAVWOHWZXOQYDB-UHFFFAOYSA-H barium(2+);2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Ba+2].[Ba+2].[Ba+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O PAVWOHWZXOQYDB-UHFFFAOYSA-H 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003593 chromogenic compound Substances 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000012847 fine chemical Substances 0.000 description 2
- 238000010230 functional analysis Methods 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 229950008679 protamine sulfate Drugs 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- YDMBNDUHUNWWRP-VJBWXMMDSA-N (2s)-1-[(2r)-2-amino-3-phenylpropanoyl]-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]piperidine-2-carboxamide Chemical compound C([C@@H](N)C(=O)N1[C@@H](CCCC1)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C1=CC=CC=C1 YDMBNDUHUNWWRP-VJBWXMMDSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241001146702 Candidatus Entotheonella factor Species 0.000 description 1
- DTGZHCFJNDAHEN-YSFUMNCJSA-N Cephaeline Natural products O(C)c1c(OC)cc2c([C@H]3N(C[C@@H](CC)[C@@H](C[C@H]4NCCc5c4cc(OC)c(O)c5)C3)CC2)c1 DTGZHCFJNDAHEN-YSFUMNCJSA-N 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 102000010911 Enzyme Precursors Human genes 0.000 description 1
- 108010062466 Enzyme Precursors Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 101800003778 Fibrinopeptide B Proteins 0.000 description 1
- 102400001063 Fibrinopeptide B Human genes 0.000 description 1
- 101500025568 Homo sapiens Saposin-D Proteins 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- MXRIRQGCELJRSN-UHFFFAOYSA-N O.O.O.[Al] Chemical compound O.O.O.[Al] MXRIRQGCELJRSN-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000010378 Pulmonary Embolism Diseases 0.000 description 1
- 108010039286 S 2238 Proteins 0.000 description 1
- 206010047249 Venous thrombosis Diseases 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 229940024545 aluminum hydroxide Drugs 0.000 description 1
- 229940024546 aluminum hydroxide gel Drugs 0.000 description 1
- SMYKVLBUSSNXMV-UHFFFAOYSA-K aluminum;trihydroxide;hydrate Chemical compound O.[OH-].[OH-].[OH-].[Al+3] SMYKVLBUSSNXMV-UHFFFAOYSA-K 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- DTGZHCFJNDAHEN-OZEXIGSWSA-N cephaeline Chemical compound N1CCC2=CC(O)=C(OC)C=C2[C@H]1C[C@H]1C[C@H]2C3=CC(OC)=C(OC)C=C3CCN2C[C@@H]1CC DTGZHCFJNDAHEN-OZEXIGSWSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- MYRIFIVQGRMHRF-OECXYHNASA-N fibrinopeptide b Chemical compound N([C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(=O)CNC(=O)[C@@H]1CCC(=O)N1 MYRIFIVQGRMHRF-OECXYHNASA-N 0.000 description 1
- 229940100689 human protein c Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960003766 thrombin (human) Drugs 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/4609—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates from reptiles
- G01N2333/4613—Snake venom
- G01N2333/462—Snake venom from Agkistrodon sp., e.g. acutase, ACTE
- G01N2333/4626—Snake venom from Agkistrodon sp., e.g. acutase, ACTE from Agkistrodon contortrix contortrix (copperhead snake); Protac (R)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96433—Serine endopeptidases (3.4.21)
- G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
- G01N2333/96461—Protein C (3.4.21.69)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- Neurosurgery (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT22049/86A IT1197891B (it) | 1986-10-17 | 1986-10-17 | Metodo per la determinazione dell'attivita' biologica della proteina c nel plasma umano, e relativi reagenti |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3734889A1 true DE3734889A1 (de) | 1988-04-21 |
Family
ID=11190720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19873734889 Withdrawn DE3734889A1 (de) | 1986-10-17 | 1987-10-12 | Verfahren zur bestimmung der funktionsspiegel von protein c in menschlichem plasma und verwandter reagenzien |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS63111469A (it) |
| DE (1) | DE3734889A1 (it) |
| FR (1) | FR2605411A1 (it) |
| IT (1) | IT1197891B (it) |
| NO (1) | NO874320L (it) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0434377A1 (en) * | 1989-12-19 | 1991-06-26 | Ortho Diagnostic Systems Inc. | Method of determining levels of extrinsic and intrinsic clotting factors and protein C |
| US5320945A (en) * | 1989-07-14 | 1994-06-14 | Boehringer Mannheim Gmbh | Method and reagent for the determination of antithrombin III |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0182929A1 (de) * | 1984-11-26 | 1986-06-04 | American Hospital Supply Corporation | Koaktivator zur Aktivierung von Protein C |
| US4849403A (en) * | 1985-05-29 | 1989-07-18 | Pentapharm Ag | Protein C activator, methods of preparation and use thereof |
-
1986
- 1986-10-17 IT IT22049/86A patent/IT1197891B/it active
-
1987
- 1987-10-12 DE DE19873734889 patent/DE3734889A1/de not_active Withdrawn
- 1987-10-14 FR FR8714212A patent/FR2605411A1/fr active Pending
- 1987-10-16 NO NO874320A patent/NO874320L/no unknown
- 1987-10-17 JP JP62262564A patent/JPS63111469A/ja active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5320945A (en) * | 1989-07-14 | 1994-06-14 | Boehringer Mannheim Gmbh | Method and reagent for the determination of antithrombin III |
| EP0434377A1 (en) * | 1989-12-19 | 1991-06-26 | Ortho Diagnostic Systems Inc. | Method of determining levels of extrinsic and intrinsic clotting factors and protein C |
| GR900100852A (en) * | 1989-12-19 | 1992-05-12 | Ortho Diagnostic Systems Inc | Method of determining levels of extrinsing and intrinsic clotting factors and protein c |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2605411A1 (fr) | 1988-04-22 |
| JPS63111469A (ja) | 1988-05-16 |
| NO874320D0 (no) | 1987-10-16 |
| IT1197891B (it) | 1988-12-21 |
| IT8622049A0 (it) | 1986-10-17 |
| NO874320L (no) | 1988-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0181465B1 (de) | Blutgerinnungshemmende Proteine, Verfahren zur Herstellung sowie deren Verwendung | |
| Bohn et al. | Eine quantitative Bestimmung von Faktor XIII mit Anti-Faktor-XIII-Serum | |
| EP0137269B1 (de) | Verfahren zur gleichzeitigen Bestimmung von Fibrinogen und Fibrinogen-Spaltprodukten im Plasma | |
| EP0236985B1 (de) | Verfahren zur photometrischen Bestimmung der Protein C- und/oder Protein S-Aktivität | |
| EP1074615A1 (de) | Verfahren zur Reindarstellung der den Blutgerinnungsfaktor VII aktivierenden Protease, ihres Proenzyms oder eines Gemisches beider Proteine mittels Affinitätschromatographie | |
| EP0696642B1 (de) | Verfahren zum Nachweis von Störungen des Protein C/Protein S-Systems | |
| EP0203509A2 (de) | Verfahren zur quantitativen Bestimmung von Protein C und Aktivatorpräparat zur Durchführung des Verfahrens | |
| DE2812943A1 (de) | Verfahren und reagens zur bestimmung des biologisch aktiven heparins im plasma | |
| EP0711838B2 (de) | Verfahren zum spezifischen Nachweis eines aktivierten Gerinnungsfaktors V mit einer erhöhten Stabilität gegenüber aktiviertem Protein C | |
| EP1074616A1 (de) | Verfahren zur Reindarstellung der den Blutgerinnungsfaktor VII aktivierenden Protease, ihres Proenzyms oder eines Gemisches beider Proteine mittels Ionenaustauscherchromatographie | |
| EP0776374B1 (de) | Verfahren und vorrichtung zur bestimmung der aktivität von enzymen in flüssigkeiten, beziehungsweise der konzentration und/oder aktivität von inhibitoren in flüssigkeiten | |
| EP0003474B1 (de) | Verfahren zur Prothrombin-Bestimmung, dazu verwendetes Reagenz und Verfahren zur Herstellung eines Faktor-Xa-Präparats | |
| EP0229234A1 (de) | Verfahren zur photometrischen Bestimmung von Protein C | |
| DE2734427B2 (de) | Verfahren zur Gewinnung von thrombinartigen Enzymen aus Schlangengiften | |
| EP0123883A2 (de) | Verfahren zur fotometrischen Bestimmung der aktivierten partiellen Thromboplastinzeit und Reagenz dazu | |
| DE68912477T2 (de) | Verfahren zum messen der biologischen wirkung von antithrombin iii und reagenzien zu dessen messung. | |
| EP0131606B1 (de) | Verfahren und reagenz zur differenzierten bestimmung von isoenzymen der alkalischen phosphatase | |
| EP0062310A1 (de) | Reagens zur optischen Bestimmung des Blutgerinnungsverhaltens | |
| DE3734889A1 (de) | Verfahren zur bestimmung der funktionsspiegel von protein c in menschlichem plasma und verwandter reagenzien | |
| EP0656424A2 (de) | Test zur Bestimmung der Empfindlichkeit gegenüber aktiviertem Protein C | |
| DE3442817A1 (de) | Verfahren und reagenz zur quantitativen bestimmung von freiem thyroxin in plasma, serum oder vollblut | |
| EP0626070B1 (de) | Verfahren zur bestimmung von hirudin und synthetischen trhombininhibitoren | |
| EP0445626B1 (de) | Funktioneller Test zur Bestimmung der Protein S-Aktivität | |
| AT397391B (de) | Verwendung von prothrombinfragmenten | |
| EP0570354B1 (de) | Verfahren zur Bestimmung von Fibrinogen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |