DE3720844C2 - - Google Patents
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- Publication number
- DE3720844C2 DE3720844C2 DE19873720844 DE3720844A DE3720844C2 DE 3720844 C2 DE3720844 C2 DE 3720844C2 DE 19873720844 DE19873720844 DE 19873720844 DE 3720844 A DE3720844 A DE 3720844A DE 3720844 C2 DE3720844 C2 DE 3720844C2
- Authority
- DE
- Germany
- Prior art keywords
- separation column
- plastic
- separation
- magnetic
- column according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
- B01J20/28023—Fibres or filaments
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D43/00—Separating particles from liquids, or liquids from solids, otherwise than by sedimentation or filtration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28009—Magnetic properties
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/034—Component parts; Auxiliary operations characterised by the magnetic circuit characterised by the matrix elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
Die erfindungsgemäße Trennsäule dient der Fraktionierung von magnetisch markierten Gemischen aus Zellen, Zellaggregaten oder Zellbestandteilen.The separation column according to the invention is used for the fractionation of magnetically marked mixtures of cells, cell aggregates or cell components.
Zellen und deren Bestandteile haben von Natur aus bis auf wenige Ausnahmen keine besonderen magnetischen Eigenschaften.Cells and their constituents naturally have up to few exceptions no special magnetic Properties.
Durch die gezielte Ankopplung von magnetischen Strukturen superparamagnetischen Mikropartikeln, Ferritin, Erythrozyten mit desoxygeniertem oder reduziertem Hämoglobin, oder sonstigen Magnetpartikeln) an bestimmte Zellen, Zellaggregate oder Zellbestandteile, können diese magnetisch markiert werden. Die Ankopplung kann sehr spezifisch über Antikörper oder über andere, an bestimmte Strukturen bin dende Substanzen erfolgen.Through the targeted coupling of magnetic structures superparamagnetic microparticles, ferritin, erythrocytes with deoxygenated or reduced hemoglobin, or other magnetic particles) to certain cells, Cell aggregates or cell components, these can be magnetic be marked. The coupling can be very specific Antibodies or other, to certain structures Ending substances take place.
Eine Auftrennung erfolgt in einem inhomogenen Magnetfeld. Das Gemisch wird in zwei Fraktionen aufgeteilt, eine, deren Elemente magnetische Strukturen gebunden haben, somit selbst magnetisierbar geworden sind, und eine unmagnetische. Die magnetische (positive) Fraktion ist oft von besonderem Interesse.Separation takes place in an inhomogeneous magnetic field. The mixture is divided into two fractions, one of which Elements bound to magnetic structures, thus themselves have become magnetizable, and a non-magnetic. The magnetic (positive) fraction is often special Interest.
Bisherige Verfahren verwenden meist nur Permanentmagnete zur Auftrennung, die an die Wandung des Gefäßes, das die zu trennende Suspension enthält, gehalten werden. Nur wenige nutzen die wesentlich stärkeren Magnetkräfte einer Hochgra dientenanordnung HGM. Bei dem besonders aus der Erzaufberei tung bekannten HGM-Verfahren erzeugen feine ferromagnetische Strukuren, wie etwa Metalldrähte, in ihrer Umgebung sehr große Magnetfeldgradienten. Sie sind in großer Menge in eine Säule gepackt. Beim Anlegen eines äußeren Magnetfeldes funktioniert die Säule wie ein Filter. Magnetisierbare Partikel werden von den Drähten angezogen und zurückgehal ten. Previous methods mostly only use permanent magnets Separation, which on the wall of the vessel, which the separating suspension contains. Only a few use the much stronger magnetic forces of a Hochgra service arrangement HGM. Especially from the ore processing tion known HGM processes produce fine ferromagnetic Structures, such as metal wires, are very much in their environment large magnetic field gradients. They are in large quantities in one Packed column. When applying an external magnetic field the column works like a filter. Magnetizable Particles are attracted to the wires and held back ten.
So wird etwa in DE 26 16 734 ein Verfahren zum Abscheiden von magnetisierbaren Mineralpartikeln beschrieben, das Trenn kammern gefüllt mit Stahlwolle verwendet.For example, DE 26 16 734 describes a method for separating Magnetizable mineral particles described the separation chambers filled with steel wool used.
In DE PS 7 34 137 wird ein Dauermagnetfilter zum Abscheiden magnetisierbarer Stoffe aus strömenden Flüssigkeiten, insbesondere von Viscose, Öl und Quecksilber beschrieben. Dabei werden in den Trennraum ragende Weicheisenteile direkt durch die Pole von Permanentmagneten gespeist, stellen also verlängerte Magnetpole dar. Zur Vermeidung von Korrosion und von magnetischen Kurzschlüssen wird vorgeschlagen, die Weicheisenteile mit einem Überzug aus keramischer Masse oder Gummi zu versehen. Dieses muß zur Vermeidung magnetischer Kurzschlüsse vor dem Zusammenfügen der Weicheisenelemente zum Filter geschehen. Derartige Separatoren sind aufwendig herzustellen und erzeugen entweder wegen relativ großer Dimensionen der magnetischen Elemente zu geringe Magnetkräfte oder weisen an Kontaktstellen zwischen Elementen zu viele unmagnetische Fangstellen auf. Die Auftrennung von Zellen oder Zellaggregaten ist hierbei überhaupt nicht beabsichtigt.In DE PS 7 34 137 a permanent magnetic filter for separation magnetizable substances from flowing liquids, described in particular by viscose, oil and mercury. Soft iron parts protruding into the separation area become direct powered by the poles of permanent magnets elongated magnetic poles. To avoid corrosion and of magnetic short circuits is suggested that Soft iron parts with a coating of ceramic mass or Rubber. This must avoid magnetic Short circuits before joining the soft iron elements to happen to the filter. Such separators are expensive manufacture and produce either because of relatively large Dimensions of the magnetic elements too small Magnetic forces or point at contact points between Elements too many non-magnetic traps. The separation of cells or cell aggregates is here not intended at all.
In WO 84 01 503 wird ein Verfahren zur magnetischen Separierung von Partikeln mittels Markierung durch chelatgebundene paramagnetische Ionen beschrieben, das auch biologische Partikel einschließt. Zur Separierung der magnetisch markierten Partikel wird eine Matrix aus unbeschichtetem ferromagnetischen Material (Edelstahldraht) eingesetzt. Derartige Anordnungen sind bekannt (siehe etwa M. D. Graham, J. Appl. Phys. Vol. 52 (3), 2578-80, (1981)), haben jedoch für biologische Partikel nur einen geringen praktischen Wert, da viele biologische Partikel, wie z. B. lebende eucaryotische Zellen an der Metalloberfläche geschädigt werden, die Matrix toxische Ionen freisetzt und auch unmarkierte Partikel unspezifisch durch mechanische Filterwirkung der Matrix zurückgehalten werden. WO 84 01 503 describes a method for magnetic Separation of particles by means of marking chelated paramagnetic ions described that too includes biological particles. To separate the magnetically marked particles becomes a matrix uncoated ferromagnetic material (stainless steel wire) used. Such arrangements are known (see for example M.D. Graham, J. Appl. Phys. Vol. 52 (3), 2578-80, (1981)) however only a small one for biological particles practical value, since many biological particles such as B. living eucaryotic cells on the metal surface be damaged, the matrix releases toxic ions and even unlabeled particles are unspecific due to mechanical Filter effect of the matrix are retained.
HGM-Anordnungen haben viele Vorzüge, im Vergleich zu anderen Verfahren:HGM orders have many advantages compared to other methods:
- - Starke Kräfte auf Partikel, auch schon mit kleinen Magneten,- Strong forces on particles, even with small ones Magnets,
- - in kleinen Volumina große Abscheideflächen,- large separation areas in small volumes,
- - keine prinzipiellen Mengenbegrenzungen, da Filter sehr groß gebaut werden können,- no basic quantity limits, because filters can be built very large,
- - schnellere und bessere Trennungen als bei anderen Verfahren.- faster and better separations than others Method.
Besonders die Kombination eines HGM Systemes mit superpara magnetischen Markierungspartikeln kann sehr effektiv funktionieren, da diese Partikel im Vergleich zu nur para magnetischen Stoffen (Ferritin, modifizierte Erythrozyten) schon bei kleinen Feldern sehr stark angezogen werden. Allerdings führt der unmittelbare Kontakt und die großen wirkenden Kräfte zwischen den Bestandteilen der magnetischen Fraktion und der Drahtoberfläche in bisher bekannten Trenn säulen zu Schädigungen der Zellmembranen oder der Zellbe standteile. Besonders Säugerzellen und Zellorganellen sind sehr empfindlich. Die Verletzungen bzw. die großen Zellver luste begrenzen bisher die Anwendbarkeit dieses Verfahrens auf paramagnetisch markierte Stoffe. Aber auch dort können bisher nur beständige, ferromagnetische Materialien mit sehr glatter Oberfläche verwendet werden. Aber gerade die Edel stähle, die sich durch ihre magnetischen Eigenschaften zur HGM-Trennung eignen, korrodieren in chloridhaltigen Trennmedien. Die nach kurzer Zeit entstehende Rauheit führt zu einer verstärkten Schädigung. Andererseits bieten gerade rauhe Fasern viele besonders starke magnetische Anheftungs punkte, korrosionsempfindliche oder toxische Materialien bessere magnetische Eigenschaften. Especially the combination of an HGM system with superpara magnetic marker particles can be very effective work because these particles are only para magnetic substances (ferritin, modified erythrocytes) be very strongly attracted even in small fields. However, the direct contact and the big ones forces acting between the components of the magnetic Fraction and the wire surface in previously known separations pillars to damage the cell membranes or the cell components. Especially mammalian cells and cell organelles are very sensitive. The injuries or the big cell ver So far, losses limit the applicability of this method on paramagnetically marked substances. But also there so far only stable, ferromagnetic materials with very smooth surface can be used. But just the noble steels, which are characterized by their magnetic properties HGM separation are suitable, corrode in chloride-containing Separation media. The roughness that arises after a short time leads to an increased damage. On the other hand, just offer rough fibers have many particularly strong magnetic attachments points, corrosion-sensitive or toxic materials better magnetic properties.
HGM-Anordnungen zur Zelltrennung verwenden bisher nur Trenn säulen, die unbehandelte oder silikonisierte Edelstahldrähte enthalten. Deshalb und durch die nur schwachen paramagne tischen Kräfte sind sie nicht effektiv nutzbar.HGM arrangements for cell separation have so far only used separation pillars, the untreated or siliconized stainless steel wires contain. Therefore and because of the only weak paramagne technical forces, they cannot be used effectively.
Kunststoffbeschichtete Säulen werden bisher nicht eingesetzt.Plastic-coated columns have not yet been used.
Die Erfindung betrifft die im Anspruch 1 angegebene Trennsäule. Besondere Ausführungsformen im Sinne des Anspruchs 1 werden durch die Ansprüche 2 bis 10 behandelt.The invention relates to that specified in claim 1 Separation column. Special embodiments in the sense of Claim 1 are treated by claims 2 to 10.
Durch die Beschichtung bzw. den Verguß der ferromagnetischen Matrix werden die oben beschriebenen Probleme beseitigt. Es kann ein zellfreundlicher Kunststoff mit glatter Oberfläche verwendet werden. Der Überzug führt zu einer Abtrennung des Mediums und der Zellen bzw. der Zellbestandteile von der magnetischen Matrix.By coating or casting the ferromagnetic Matrix eliminates the problems described above. It can be a cell-friendly plastic with a smooth surface be used. The coating leads to a separation of the Medium and the cells or the cell components of the magnetic matrix.
Die ferromagnetische Struktur wird durch den Verguß mechanisch stablisiert, nichtmagnetische Fangstellen (enge Spalten etc.) werden verschlossen.The ferromagnetic structure is made by potting mechanically stabilized, non-magnetic traps (narrow Gaps etc.) are closed.
Besonders in Kombination mit superparamagnetischen Markier ungspartikeln ermöglicht die erfindungsgemäße Trennsäule außerordentlich gute Trennergebnisse bei sehr geringer Zellschädigung. Especially in combination with superparamagnetic markers The separation column according to the invention enables particles to form extraordinarily good separation results with very little Cell damage.
Eine 2 ml Insulinspritze (Durchmesser 6,5 mm) wird auf eine Länge von 20 mm mit 150 mg Cr-Mo-Stahlwolle (Werkstoff 1.4113S, Faserdurchmesser 60 µm) gefüllt. Die Hauptfaserrich tung fällt mit der Achse der Spritze zusammen. Die Stahl wolle wird zunächst benetzt, indem man Lackverdünner(Lesonal V83) durch die Fasern aufzieht. Man läßt den Verdünner ablaufen und zieht anschließend mehrmals angerührten 2- Komponenten Klarlack (Lesonal K86) durch die Wolle. Nach Abtropfen des überschüssigen Lacks zentrifugiert man die Spritze dann bei 100 · g für 1 Minute.A 2 ml insulin syringe (diameter 6.5 mm) is placed on one Length of 20 mm with 150 mg Cr-Mo steel wool (material 1.4113S, fiber diameter 60 µm). The main grain tion coincides with the axis of the syringe. The steel wool is first wetted by using lacquer thinner (Lesonal V83) through the fibers. The thinner is left run off and then pulls the 2- Components clear coat (Lesonal K86) through the wool. To The excess paint is drained off and centrifuged Then syringe at 100 x g for 1 minute.
Nach 2 Tagen ist der Lack getrocknet. Im Wasserstrahlvakuum wird die innere Kunststoffoberfläche nun silikonisiert. Die nun fertige Trennsäule hat eine Kapazität von 20 cm2, aus reichend für ca. 107 Zellen (z. B. Lymphozyten). Sie kann autoklaviert und wiederverwertet werden.The varnish has dried after 2 days. The inner plastic surface is now siliconized in a water jet vacuum. The now finished separation column has a capacity of 20 cm 2 , sufficient for approx. 10 7 cells (e.g. lymphocytes). It can be autoclaved and reused.
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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DE19873720844 DE3720844A1 (en) | 1987-06-24 | 1987-06-24 | Column for the magnetic separation of cells, cell aggregates and cellular constituents |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19873720844 DE3720844A1 (en) | 1987-06-24 | 1987-06-24 | Column for the magnetic separation of cells, cell aggregates and cellular constituents |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3720844A1 DE3720844A1 (en) | 1989-01-05 |
DE3720844C2 true DE3720844C2 (en) | 1991-01-10 |
Family
ID=6330187
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE19873720844 Granted DE3720844A1 (en) | 1987-06-24 | 1987-06-24 | Column for the magnetic separation of cells, cell aggregates and cellular constituents |
Country Status (1)
Country | Link |
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DE (1) | DE3720844A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10144291A1 (en) * | 2001-09-08 | 2003-03-27 | Stefan Schuetze | Apparatus to isolate biological material, bonded to magnetic particles, has a chamber with a wall and a membrane against a magnet array which gives a focused magnetic field to hold them while the residue is flushed away |
EP2444158A2 (en) | 2010-10-20 | 2012-04-25 | Miltenyi Biotec GmbH | Device for fragmenting tissue |
US9068991B2 (en) | 2009-06-08 | 2015-06-30 | Singulex, Inc. | Highly sensitive biomarker panels |
US9239284B2 (en) | 2007-12-19 | 2016-01-19 | Singulex, Inc. | Scanning analyzer for single molecule detection and methods of use |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6020210A (en) | 1988-12-28 | 2000-02-01 | Miltenvi Biotech Gmbh | Methods and materials for high gradient magnetic separation of biological materials |
US5439586A (en) * | 1993-09-15 | 1995-08-08 | The Terry Fox Laboratory Of The British Columbia Cancer Agnecy | Magnetic filter with ordered wire array |
US5503801A (en) * | 1993-11-29 | 1996-04-02 | Cobe Laboratories, Inc. | Top flow bubble trap apparatus |
US5591251A (en) * | 1993-11-29 | 1997-01-07 | Cobe Laboratories, Inc. | Side flow bubble trap apparatus and method |
US5705059A (en) | 1995-02-27 | 1998-01-06 | Miltenyi; Stefan | Magnetic separation apparatus |
US6190870B1 (en) * | 1995-08-28 | 2001-02-20 | Amcell Corporation | Efficient enrichment and detection of disseminated tumor cells |
US8685711B2 (en) | 2004-09-28 | 2014-04-01 | Singulex, Inc. | Methods and compositions for highly sensitive detection of molecules |
US9040305B2 (en) | 2004-09-28 | 2015-05-26 | Singulex, Inc. | Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry |
WO2011140484A1 (en) | 2010-05-06 | 2011-11-10 | Singulex, Inc | Methods for diagnosing, staging, predicting risk for developing and identifying treatment responders for rheumatoid arthritis |
DE102021214281A1 (en) * | 2021-12-14 | 2023-06-15 | Robert Bosch Gesellschaft mit beschränkter Haftung | Device and method for splitting three-dimensional agglomerates |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE734137C (en) * | 1938-09-21 | 1943-04-08 | Deutsche Edelstahlwerke Ag | Permanent magnetic filter for separating magnetizable substances from flowing liquids |
US3700555A (en) * | 1970-10-12 | 1972-10-24 | Technicon Instr | Method and apparatus for lymphocyte separation from blood |
DE2616734A1 (en) * | 1975-04-16 | 1976-10-28 | English Clays Lovering Pochin | METHOD AND APPARATUS FOR MODIFYING THE EFFECTIVE SIZE OF PARTICLES OF VARIOUS MAGNETIC SUSPENSIONS MIXED WITH EACH OTHER AND SUSPENDED IN A FLUID |
US4508625A (en) * | 1982-10-18 | 1985-04-02 | Graham Marshall D | Magnetic separation using chelated magnetic ions |
-
1987
- 1987-06-24 DE DE19873720844 patent/DE3720844A1/en active Granted
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10144291A1 (en) * | 2001-09-08 | 2003-03-27 | Stefan Schuetze | Apparatus to isolate biological material, bonded to magnetic particles, has a chamber with a wall and a membrane against a magnet array which gives a focused magnetic field to hold them while the residue is flushed away |
DE10144291C2 (en) * | 2001-09-08 | 2003-10-09 | Stefan Schuetze | Device for isolating biological material bound to magnetic particles in a magnetic field |
US9239284B2 (en) | 2007-12-19 | 2016-01-19 | Singulex, Inc. | Scanning analyzer for single molecule detection and methods of use |
US9068991B2 (en) | 2009-06-08 | 2015-06-30 | Singulex, Inc. | Highly sensitive biomarker panels |
EP2444158A2 (en) | 2010-10-20 | 2012-04-25 | Miltenyi Biotec GmbH | Device for fragmenting tissue |
DE102010042723A1 (en) | 2010-10-20 | 2012-04-26 | Miltenyi Biotec Gmbh | Apparatus and method for separating Neél and Brown magnetic particles |
Also Published As
Publication number | Publication date |
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DE3720844A1 (en) | 1989-01-05 |
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Owner name: MILTENYI, STEFAN, 51429 BERGISCH GLADBACH, DE |
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8381 | Inventor (new situation) |
Free format text: MILTENYI, STEFAN, 51429 BERGISCH GLADBACH, DE RADBRUCH, ANDREAS, DR., 53123 BONN, DE WEICHEL, WALTER, 51105 KOELN, DE MUELLER, WERNER, DR., 50931 KOELN, DE GOETTLINGER, CHRISTOPH, 50829 KOELN, DE MEYER, KLAUS LUDWIG, 50259 PULHEIM, DE |
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8327 | Change in the person/name/address of the patent owner |
Owner name: MILTENYI BIOTEC GMBH, 51429 BERGISCH GLADBACH, DE |