DE10144291A1 - Apparatus to isolate biological material, bonded to magnetic particles, has a chamber with a wall and a membrane against a magnet array which gives a focused magnetic field to hold them while the residue is flushed away - Google Patents

Apparatus to isolate biological material, bonded to magnetic particles, has a chamber with a wall and a membrane against a magnet array which gives a focused magnetic field to hold them while the residue is flushed away

Info

Publication number
DE10144291A1
DE10144291A1 DE2001144291 DE10144291A DE10144291A1 DE 10144291 A1 DE10144291 A1 DE 10144291A1 DE 2001144291 DE2001144291 DE 2001144291 DE 10144291 A DE10144291 A DE 10144291A DE 10144291 A1 DE10144291 A1 DE 10144291A1
Authority
DE
Germany
Prior art keywords
chamber
magnetic field
wall
membrane
biological material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE2001144291
Other languages
German (de)
Other versions
DE10144291C2 (en
Inventor
Stefan Schuetze
Vladimir Tchikov
Dietrich Kabelitz
Martin Kroenke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE2001144291 priority Critical patent/DE10144291C2/en
Publication of DE10144291A1 publication Critical patent/DE10144291A1/en
Application granted granted Critical
Publication of DE10144291C2 publication Critical patent/DE10144291C2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/032—Matrix cleaning systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Reciprocating Pumps (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

An apparatus to isolate biological material bonded to magnetic particles, in a magnetic field, has a chamber (10) and an array of magnets (12,17), is new. The chamber is defined by a frame, with one surface as a membrane (16) and the other surface as a wall (18). The magnet assembly has a number of parallel ferrous half-rods (17) lying against the membrane, focusing the magnetic field. An apparatus to isolate biological material bonded to magnetic particles, in a magnetic field, has a chamber (10) and an array of magnets (12,17), is new. The chamber is defined by a frame, with one surface as a membrane (16) and the other surface as a wall (18). The magnet assembly has a number of parallel ferrous half-rods (17) lying against the membrane, focusing the magnetic field. The membrane and the wall can take up different temperatures through a fluid convection within the chamber. A pump system gives a flushing flow through the chamber.

Description

Die Erfindung betrifft eine Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Material in einem Magnetfeld, die mit einer Kammer und einem Magneten versehen ist. The invention relates to a device for insulation of biological bound to magnetic particles Material in a magnetic field with a chamber and is provided with a magnet.

Eine derartige Vorrichtung ist aus der DE 37 20 844 C2 bekannt. Bei dieser Vorrichtung ist in der Kammer eine magnetische Struktur vorgesehen, an der das an magnetischen Partikeln gebundene biologische Material anhaften soll. Bei dieser Vorrichtung ist das "Ernten" des isolierten biologischen Materials schwierig. Aus der DE 39 25 093 C2 ist eine weitere Vorrichtung der eingangs genannten Art bekannt, bei der die magnetischen Kraftlinien bereits senkrecht zur Bewegungsrichtung der biologischen Partikel in der Trennkammer verlaufen. Such a device is from DE 37 20 844 C2 known. In this device there is one in the chamber provided magnetic structure to which the biological particles bound to magnetic particles should. In this device, the "harvesting" of the isolated biological material difficult. From the DE 39 25 093 C2 is another device at the beginning known type, in which the magnetic lines of force already perpendicular to the direction of movement of the biological Particles run in the separation chamber.

Eine hohe Spezifität, wie sie erforderlich ist, um kleinste, subzelluläre Teilchen zu selektieren, setzt das Vorhandensein eines hohen Magnetfelds voraus. Der Erfindung liegt die Aufgabe zugrunde, eine Vorrichtung zu schaffen, die es ermöglicht, an magnetische Partikeln gebundenes biologisches Material mit einer hohen Ausbeute und hohem Reinheitsgrad zu gewinnen. A high level of specificity as required to Selecting the smallest, subcellular particles sets that Presence of a high magnetic field ahead. The invention is based on the task of creating a device which allows it to be bound to magnetic particles biological material with a high yield and high Gain purity.

Erfindungsgemäß wird diese Aufgabe gelöst durch eine Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Material in einem Magnetfeld, mit einer Kammer und einem Magnetsystem, bei der die Kammer durch einen Rahmen definiert wird, dessen eine Fläche durch eine dünne Membran und dessen andere Fläche durch eine Wandung abgedeckt ist, das Magnetsystem eine Vielzahl von parallel zueinander verlaufenden, das Magnetfeld fokussierenden Eisen-Halbstäben trägt, die an der dünnen Membran anliegen, und wobei die Membran und die Wandung unter Ausbildung einer Konvektion des Fluids in der Kammer unterschiedliche Temperaturen annehmen können. According to the invention, this object is achieved by Device for isolating magnetic particles bound biological material in a magnetic field, with a chamber and a magnet system in which the chamber is defined by a frame, one surface of which through a thin membrane and its other surface one wall is covered, the magnet system one Variety of parallel to each other, the magnetic field focusing iron half-rods that bears on the thin Apply membrane, and where the membrane and the wall forming a convection of the fluid in the Chamber can assume different temperatures.

Eine bevorzugte Ausgestaltung zeichnet sich aus durch ein einen Spülstrom in der Kammer bewirkendes Pumpsystem. A preferred embodiment is characterized by pumping system causing a purge flow in the chamber.

Der Erfindung liegt die Erkenntnis zugrunde, daß bei einem sehr hoch fokussierten Magnetfeld, in dessen Nähe die magnetisierten Partikel durch Konvektion vorbeigeführt werden, diese Partikel im Bereich des Magnetfeldes fixiert werden. The invention is based on the finding that a very highly focused magnetic field, in the vicinity of which magnetized particles passed by convection these particles in the area of the magnetic field be fixed.

Die Erfindung wird anhand einer Zeichnung erläutert. Dabei zeigt: The invention is explained with reference to a drawing. It shows:

Fig. 1a das der Erfindung zugrundeliegende Prinzip, FIG. 1a, the object underlying the invention principle,

Fig. 1b den Spülvorgang, FIG. 1b, the flushing operation,

Fig. 2 eine Draufsicht auf die Vorrichtung und Fig. 2 is a plan view of the device and

Fig. 3 eine Schnittdarstellung durch die Vorrichtung. Fig. 3 is a sectional view through the device.

Die Vorrichtung besteht aus einem Rahmen 14, der die Kammer 10 bildet. In dem Rahmen sind mehrere Zuflüsse (durch nach innen zeigende Pfeile markiert) und Abflüsse (durch nach außen weisende Pfeile markiert) vorgesehen. The device consists of a frame 14 which forms the chamber 10 . Several inflows (marked by arrows pointing inwards) and outflows (marked by arrows pointing outwards) are provided in the frame.

Die eine Rahmenfläche ist durch eine dünne Membran 16, die andere Rahmenfläche durch eine Wandung 18 - vorzugsweise eine andere dünne Membran oder ein Glas - abgedeckt. One frame surface is covered by a thin membrane 16 , the other frame surface by a wall 18 - preferably another thin membrane or a glass.

Der Magnet 12 trägt eine Vielzahl von parallel zueinander verlaufenden Eisen-Halbstäben 17 (in der Zeichnung sind nur drei dargestellt, tatsächlich aber sind weit mehr Eisen-Halbstäbe 17 vorgesehen). Diese Eisen-Halbstäbe 17, die einen Durchmesser von etwa 0,5 mm, vorzugweise 0,3 mm haben, liegen an der dünnen Membran 16 unmittelbar an. Die Eisen-Halbstäbe 17 dienen dazu, das Magnetfeld zu fokussieren, das Magnetfeld ist also im Bereich ihres Kontakts zu der dünnen Membran 16 besonders groß. The magnet 12 carries a plurality of iron half-bars 17 running parallel to one another (only three are shown in the drawing, but in fact far more iron half-bars 17 are provided). These iron half-bars 17 , which have a diameter of approximately 0.5 mm, preferably 0.3 mm, rest directly on the thin membrane 16 . The iron half-bars 17 serve to focus the magnetic field, so the magnetic field is particularly large in the area of their contact with the thin membrane 16 .

In der Kammer 10 entsteht bei Bestehen einer Temperaturdifferenz zwischen der Membran 16 und der Wandung 18 eine Konvektion (das Magnetsystem 12, 17 mit der Membran 16 werden konstant nahe des Schmelzpunktes (4°C) gehalten, während die gegenüberliegende Wandung 18 nahe einer Kühlschranktemperatur (nahe 8°C) gehalten wird, wodurch der Temperarurgradient durch die thermische Leitfähigkeit durch die Rahmenflächen als auch durch das Fluid in der Kammer 10 erhalten wird). Auf diese Weise wird das in die Kammer eingebrachte biologische Material zirkulieren, das an magnetische Partikel gebundene biologische Material setzt sich auf der dünnen Membran 16 dort, wo diese auf der gegenüberliegenden Seite mit den Eisen-Halbstäben 17 in Kontakt ist, ab. Nach Auswaschen des nicht an magnetische Partikel gebundenen biologischen Materials aus der Kammer - das an magnetische Partikel gebundene biologische Material bleibt von dem Magnetfeld in dem Bereich der Eisen-Halbstäbe haften - verbleibt das zu isolierende biologische Material in der Kammer und kann - etwa durch Pipettieren - gewonnen werden. If there is a temperature difference between the membrane 16 and the wall 18, convection occurs in the chamber 10 (the magnet system 12 , 17 with the membrane 16 are kept constant near the melting point (4 ° C.), while the opposite wall 18 is close to a refrigerator temperature ( is kept close to 8 ° C), whereby the temperature gradient is obtained through the thermal conductivity through the frame surfaces as well as through the fluid in the chamber 10 ). In this way, the biological material introduced into the chamber will circulate, and the biological material bound to magnetic particles will settle on the thin membrane 16 where it contacts the iron half-rods 17 on the opposite side. After washing the biological material that is not bound to magnetic particles out of the chamber - the biological material bound to magnetic particles remains adhering to the magnetic field in the area of the iron half-rods - the biological material to be isolated remains in the chamber and can be - for example by pipetting - be won.

Claims (2)

1. Vorrichtung zur Isolierung von an magnetischen Partikeln gebundenem biologischen Material in einem Magnetfeld, mit einer Kammer (10) und einem Magnetsystem (12, 17), dadurch gekennzeichnet, daß
die Kammer (10) durch einen Rahmen (14) definiert ist, dessen eine Fläche durch eine dünne Membran (16) und dessen andere Fläche durch eine Wandung (18) abgedeckt ist,
das Magnetsystem (12, 17) eine Vielzahl von parallel zueinander verlaufenden, das Magnetfeld fokussierenden Eisen-Halbstäben (17) aufweist, die an der dünnen Membran (16) anliegen, und
wobei die Membran (16) und die Wandung (18) unter Ausbildung einer Konvektion des Fluids in der Kammer (10) unterschiedliche Temperaturen annehmen können. 1. Device for isolating biological material bound to magnetic particles in a magnetic field, with a chamber ( 10 ) and a magnet system ( 12 , 17 ), characterized in that
the chamber ( 10 ) is defined by a frame ( 14 ), one surface of which is covered by a thin membrane ( 16 ) and the other surface of which is covered by a wall ( 18 ),
the magnet system ( 12 , 17 ) has a plurality of iron half-rods ( 17 ) which run parallel to one another and focus the magnetic field and which abut the thin membrane ( 16 ), and
wherein the membrane ( 16 ) and the wall ( 18 ) can assume different temperatures while forming a convection of the fluid in the chamber ( 10 ). 2. Vorrichtung nach Anspruch 1, gekennzeichnet durch ein einen Spülstrom (15) in der Kammer (10) bewirkendes Pumpsystem. 2. Device according to claim 1, characterized by a flushing flow ( 15 ) in the chamber ( 10 ) effecting pump system.
DE2001144291 2001-09-08 2001-09-08 Device for isolating biological material bound to magnetic particles in a magnetic field Expired - Fee Related DE10144291C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE2001144291 DE10144291C2 (en) 2001-09-08 2001-09-08 Device for isolating biological material bound to magnetic particles in a magnetic field

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2001144291 DE10144291C2 (en) 2001-09-08 2001-09-08 Device for isolating biological material bound to magnetic particles in a magnetic field

Publications (2)

Publication Number Publication Date
DE10144291A1 true DE10144291A1 (en) 2003-03-27
DE10144291C2 DE10144291C2 (en) 2003-10-09

Family

ID=7698332

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2001144291 Expired - Fee Related DE10144291C2 (en) 2001-09-08 2001-09-08 Device for isolating biological material bound to magnetic particles in a magnetic field

Country Status (1)

Country Link
DE (1) DE10144291C2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4440638A (en) * 1982-02-16 1984-04-03 U.T. Board Of Regents Surface field-effect device for manipulation of charged species
DE3720844C2 (en) * 1987-06-24 1991-01-10 Stefan 5060 Bergisch Gladbach De Miltenyi
WO1993020927A1 (en) * 1992-04-16 1993-10-28 British Technology Group Ltd. Apparatus for separating a mixture
DE3925093C2 (en) * 1989-07-28 1995-03-30 Roland Dipl Phys Hartig Device for separating biological material bound to magnetic particles (beads) in a magnetic field
WO1999062622A1 (en) * 1998-05-29 1999-12-09 Industrial Research Limited Method and apparatus for concentrating and/or positioning particles or cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4440638A (en) * 1982-02-16 1984-04-03 U.T. Board Of Regents Surface field-effect device for manipulation of charged species
DE3720844C2 (en) * 1987-06-24 1991-01-10 Stefan 5060 Bergisch Gladbach De Miltenyi
DE3925093C2 (en) * 1989-07-28 1995-03-30 Roland Dipl Phys Hartig Device for separating biological material bound to magnetic particles (beads) in a magnetic field
WO1993020927A1 (en) * 1992-04-16 1993-10-28 British Technology Group Ltd. Apparatus for separating a mixture
WO1999062622A1 (en) * 1998-05-29 1999-12-09 Industrial Research Limited Method and apparatus for concentrating and/or positioning particles or cells

Also Published As

Publication number Publication date
DE10144291C2 (en) 2003-10-09

Similar Documents

Publication Publication Date Title
DE60019761T2 (en) APPARATUS AND METHOD FOR DIELECTROPHORESIS
DE60311210T2 (en) Microfluidic pump system
US20070152206A1 (en) Device for manipulating particles using dielectrophoresis employing metal-post electrode structure and method of manipulating particles using the device at high flow rate
WO2000000292A1 (en) Electrode arrangement for the dielectrophoretic diversion of particles
EP1333932B1 (en) Method for separating a dispersed or dissolved substance and magnet separator
DE102008020428B3 (en) Apparatus and method and gel system for analytical and preparative electrophoresis
WO2007118261A1 (en) Apparatus for enhancing the reaction efficiency, especially the binding efficiency, between molecules and molecular moieties
EP1979738B1 (en) Arrangement for generating liquid flows and/or particle flows, method for producing and operating said arrangement and use of the latter
EP1147803B1 (en) Filtration and separation apparatus, especially for biological-organic fluids
CN1305545C (en) Method and equipment for compressing a dispersion in liquid-liquid extraction
DE10144291A1 (en) Apparatus to isolate biological material, bonded to magnetic particles, has a chamber with a wall and a membrane against a magnet array which gives a focused magnetic field to hold them while the residue is flushed away
Xu et al. Three-dimensional (3D) hydrodynamic focusing for continuous sampling and analysis of adherent cells
EP1609880B1 (en) Sputtering cathod for coating methods
DE10135091A1 (en) Method, device and slide for microdissection
CN113265327A (en) Alternating current-dielectrophoresis microalgae multistage sorting device and method based on algae lipid content
EP1744831B1 (en) Method and device for collecting suspended particles
Jimenez et al. Microfluidics for effective concentration and sorting of waterborne protozoan pathogens
DE112018007860T5 (en) Biochemical cassette and biochemical analyzer
EP4326439A1 (en) Method and device for trapping at least one nucleated cell using at least one electrode for a microfluidic device
Schor et al. Rapid fabrication of three-dimensional structures for dielectrophoretic sorting of lipid-containing organisms
DE3925093C2 (en) Device for separating biological material bound to magnetic particles (beads) in a magnetic field
EP3594187B1 (en) Use of a purification device for water treatment
DE112019004459T5 (en) ENRICHMENT OF SAMPLES BY MEANS OF MAGNETIC PARTICLES IN MICROCHANNELS
DE2012751C3 (en) Hydrodynamic separation of impurities for a liquid used for light modulation
DE69938188T2 (en) Electrode design for electric field flux fractionation

Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
8304 Grant after examination procedure
8364 No opposition during term of opposition
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee

Effective date: 20150401