DE3202559A1 - Verfahren zum nachweis von igm-antikoerpern gegen hepatitis a-virus und reagens zur durchfuehrung von immunoassays - Google Patents
Verfahren zum nachweis von igm-antikoerpern gegen hepatitis a-virus und reagens zur durchfuehrung von immunoassaysInfo
- Publication number
- DE3202559A1 DE3202559A1 DE19823202559 DE3202559A DE3202559A1 DE 3202559 A1 DE3202559 A1 DE 3202559A1 DE 19823202559 DE19823202559 DE 19823202559 DE 3202559 A DE3202559 A DE 3202559A DE 3202559 A1 DE3202559 A1 DE 3202559A1
- Authority
- DE
- Germany
- Prior art keywords
- igm
- hepatitis
- reagent
- virus
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000001514 detection method Methods 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 10
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 9
- 238000003018 immunoassay Methods 0.000 title claims description 6
- 241000709721 Hepatovirus A Species 0.000 title claims description 5
- 208000006454 hepatitis Diseases 0.000 claims description 24
- 231100000283 hepatitis Toxicity 0.000 claims description 20
- 210000002966 serum Anatomy 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 13
- 239000007787 solid Substances 0.000 claims description 11
- 239000000427 antigen Substances 0.000 claims description 7
- 102000036639 antigens Human genes 0.000 claims description 7
- 108091007433 antigens Proteins 0.000 claims description 7
- 241000700605 Viruses Species 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 230000035945 sensitivity Effects 0.000 claims description 3
- 239000011324 bead Substances 0.000 description 13
- 239000000523 sample Substances 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 4
- 208000005252 hepatitis A Diseases 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 239000012898 sample dilution Substances 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000288961 Saguinus imperator Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- RCRODHONKLSMIF-UHFFFAOYSA-N isosuberenol Natural products O1C(=O)C=CC2=C1C=C(OC)C(CC(O)C(C)=C)=C2 RCRODHONKLSMIF-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 241001515942 marmosets Species 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5768—Hepatitis A
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Communicable Diseases (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22925081A | 1981-01-28 | 1981-01-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3202559A1 true DE3202559A1 (de) | 1982-08-05 |
Family
ID=22860424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19823202559 Ceased DE3202559A1 (de) | 1981-01-28 | 1982-01-27 | Verfahren zum nachweis von igm-antikoerpern gegen hepatitis a-virus und reagens zur durchfuehrung von immunoassays |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPS57144462A (enrdf_load_stackoverflow) |
| BE (1) | BE891901A (enrdf_load_stackoverflow) |
| CA (1) | CA1172561A (enrdf_load_stackoverflow) |
| DE (1) | DE3202559A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2498760B1 (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0507587A3 (en) * | 1991-04-03 | 1993-03-03 | Syntex (U.S.A.) Inc. | Immunoassay for immunoglubulins |
| EP0781998A3 (de) * | 1995-12-27 | 1998-08-12 | Dade Behring Marburg GmbH | Immunologische Bestimmungsmethode |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1240937A (en) * | 1982-07-26 | 1988-08-23 | Gordon R. Dreesman | Monoclonal igm antibodies and method of preparation |
| FR2917173B1 (fr) * | 2007-06-08 | 2009-07-31 | Bio Rad Pasteur Sa | Procede multiplexe de detection d'une infection |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1059901A (en) * | 1974-06-17 | 1979-08-07 | Chung-Mei Ling | Direct radioimmunoassay for antigens and their antibodies |
| US3992517A (en) * | 1975-02-19 | 1976-11-16 | Pfizer Inc. | Detection of hepatitis B surface antigen by latex agglutination |
| US4273756A (en) * | 1978-07-28 | 1981-06-16 | Abbott Laboratories | Immunoassay for class specific antibodies |
| US4464474A (en) * | 1980-07-09 | 1984-08-07 | Connaught Laboratories Limited | Non-A, non-B hepatitis assay and vaccine |
-
1982
- 1982-01-18 CA CA000394315A patent/CA1172561A/en not_active Expired
- 1982-01-26 BE BE0/207141A patent/BE891901A/fr not_active IP Right Cessation
- 1982-01-27 DE DE19823202559 patent/DE3202559A1/de not_active Ceased
- 1982-01-27 FR FR8201260A patent/FR2498760B1/fr not_active Expired
- 1982-01-28 JP JP57011053A patent/JPS57144462A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0507587A3 (en) * | 1991-04-03 | 1993-03-03 | Syntex (U.S.A.) Inc. | Immunoassay for immunoglubulins |
| EP0781998A3 (de) * | 1995-12-27 | 1998-08-12 | Dade Behring Marburg GmbH | Immunologische Bestimmungsmethode |
Also Published As
| Publication number | Publication date |
|---|---|
| CA1172561A (en) | 1984-08-14 |
| BE891901A (fr) | 1982-07-26 |
| FR2498760B1 (fr) | 1986-04-04 |
| JPH0368346B2 (enrdf_load_stackoverflow) | 1991-10-28 |
| JPS57144462A (en) | 1982-09-07 |
| FR2498760A1 (fr) | 1982-07-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8131 | Rejection |