DE302911C - - Google Patents

Description

IMPERIAL

PATENT OFFICE.

By patent 300513 is a method for the preparation of heavy metal compounds of Proteins or their breakdown products protected, which consists in that one Heavy metal compounds or colloidal heavy metals on such proteins or whose degradation products can act, which result from pathologically or physiologically changed or infected animal or human borrowed organs or tissues are obtained. The heavy metal compounds that can be produced using this method have proven to be valuable tools for the diagnosis and therapeutic treatment of such physiological and pathological changes in the human and animal organism that occur on the The presence of protein-degrading ferments has been proven.

In a further development of the banned procedure it has now been found that one too by the action of metals or metal compounds on such protein compounds from normal, animal or human organs or tissues or pressed juices that according to known processes as far from all water-soluble components as degradation products etc., are exempt from showing that they no longer show a ninhydrin reaction, to new heavy metal protein compounds which differs from the previously known metal-protein compounds, which are made with ninhydrin reacting protein bodies from normal organs or tissues were presented, to an excellent degree for therapeutic purposes Suitable for use. While namely those shown from normal and not further pretreated organ proteins Metal-protein compounds when used on humans and animals in addition to the desired specific effect still cause severe disruptive side effects, this applies for those made from protein substances that have been pretreated to the point of non-hydrin reaction, rather, these show only the desired specific, without any side effects Healing effect. Proteins from normal, animal or human organs, which up to have been prepurified to prevent the ninhydrin reaction, have so far been used for production heavy metal compounds have not yet been used at all.

The representation of the new heavy metal protein compounds is carried out according to the Main patent described method in such a way that one heavy metal compounds or colloidal · heavy metals based on such proteins from normal human or animal organs can act, which by known methods, for. B. by continuous Boiling with water as far as water-soluble components such as degradation products etc. are exempt from the fact that they no longer react with triketohydroindene hydrate (ninhydrin) (see Journal of the former Society 97,

[1910], pp. 2027/28, 2030/31; Journal of Physiological Chemie 72, [1-911], pp. 37 to 43). Of the Excess of the metal used must also be removed again here in a suitable manner will. The heavy metal protein compounds shown in this way, which the metal in masked or non-ionized bond will come into contact with protein-degrading Ferments specifically broken down, whereby the heavy metal is set free and can be detected by the usual reagents. The new bodies can, like those of the main patent, for diagnostic and therapeutic purposes Find use.

Example 1.
Representation of the iron-lung protein connection.

An animal or human lung is bled, crushed, defatted and boiled with distilled water according to known methods until Y ₂ l of washing water concentrated to ι ecm with 1 ecm of an i% ninhydrin solution no longer reacts.

The pulmonary protein compound boiled out in this way, after it has been pressed through a fine sieve, washed up with a little water and mixed with a 3% ferric chloride solution in excess with vigorous stirring and left to stand for a few hours. The iron-lung protein compound formed is then washed out with water until the washing water is completely free of iron. This compound is then brought in acetone for _{4 hours;} After the acetone is filtered off with suction, is dried in high vacuum at 70 ⁰ C water bath temperature for 3 hours, triturated then in a Keibschale, sieved through a fine metal screen, the connection to 0,1 fills g in tubes made of alkali-free Jena glass melts to and sterilized at 102 ° C for 2 hours.

The pure iron-lung protein compound represented in this way is a fine, brownish powder, which contains iron in a non-ionized state, and that in water and the common organic solvents is insoluble.

Example 2.
Representation of the copper-brain protein connection.

An animal or human brain is bled, chopped up, defatted and boiled with distilled water according to known methods until ^x / ₂ l of the washing water concentrated to ι ecm with 1 ecm of a 1 percent ninhydrin solution no longer reacts. The brain protein prepared in this way is pressed through a fine sieve, washed up with a little water and mixed with an excess of a 20 percent copper sulfate solution and left to stand for 4 hours. The copper-brain protein compound obtained is then washed with water until the washing water no longer shows any copper reaction. The same is then suspended in acetone for some time, the acetone is removed by suction and then ^{dried in a high vacuum at 70 °} C. water bath temperature. After trituration, the copper-brain protein compound obtained is obtained in the form of a greenish-gray powder which contains the copper in a masked or non-ionized bond and which is insoluble in water and the organic solvents used.

Example 3.
Representation of the silver-liver protein compound.

An animal or human liver is after bleeding, mincing and defatting according to the procedure described in the preceding examples until the ninhydrin reaction does not occur with distilled water boiled out and the liver-protein compound freed from degradation products, etc. pressed through a fine sieve, suspended in water and mixed with an excess of a 10 percent silver nitrate solution good stirring added. After standing for several hours, the resulting silver - liver protein compound becomes Washed with water until the wash water is free of silver. The dried in the manner described in Example 1 and sterilized compound is a fine gray-white powder that -in water and is insoluble in common organic solvents.

Example 4.

Representation of the mercury protein compound from thyroid glands.

The thyroid are crushed in a known manner, bled and as long boiled with distilled water until the ^{cooking: -} 105 after concentrating no longer shows ninhydrin reaction liquid. An excess of 2.5 percent mercury chloride solution is then added to the organs suspended in water and left to stand for a few hours, then washed with water until the excess mercury salt is removed, dehydrated with acetone, dried in vacuo, pulverized, sieved and sterilized one hour at 105 ^° C. The pure mercury-silver compound from thyroid protein is a fine yellowish-white powder which contains the mercury in non-ionized form and which is insoluble in water and most organic solvents.

Instead of the pretreatment mentioned in the examples until ninhydrin reaction-free

th protein compounds can analogously pretreated protein compounds from other animal or human organs or tissues converted into metal compounds which generally have similar properties. The ones given in the examples Metals or metal salts can be replaced by other metal compounds, such as. B. those of cadmium, chromium, gold,

ίο of zinc, cobalt, etc., are replaced, whereby the corresponding heavy metal protein compounds are obtained.

Example 1 5.

Representation of the cadmium-testicular protein compound.

Animal or human testes are bled, crushed, defatted and boiled with distilled water according to known methods until ¹ J ₂ 1 of the washing water concentrated to ι ecm with 1 ecm of an I-percent ninhydrin solution no longer reacts. The testicle protein prepared in this way is pressed through a fine sieve, with little

Suspended water and with an excess of a 10 percent cadmium chloride solution added and left to stand for a few hours. The obtained cadmium testicular protein compound is now washed with water until the wash water no longer shows any cadmium reaction. On that if the compound is suspended in acetone for some time, the acetone is removed by suction and then dried in a high vacuum at 70 ° water bath temperature. After rubbing in, the cadmium testis - Protein compound obtained in the form of a gray powder, which masked the cadmium or non-ionized bond, and that in water and the common organic Is insoluble in solvents.

Example 6.

Representation of the chromium muscle protein

binding.

Animal or human muscles are bled, crushed, defatted and boiled with distilled water according to known methods until ^a / ₂ l of the wash water concentrated to 1 ecm with 1 ecm of a 1 percent ninhydrate solution no longer reacts. The so. Boiled muscle protein compound is washed up with a little water after it has been pressed through a fine sieve and mixed with an excess of 15 percent chromium-alum solution with vigorous stirring and left to stand for a few hours. The chromium-muscle protein compound formed is then washed out with water until the wash water is completely free of chromium. This compound Y is placed in acetone for _{4 hours;} after the acetone has been sucked off, it is dried. in a high vacuum at 70 ° water bath temperature for 3 hours, then rubbed in a mortar, sifted through a close-meshed metal sieve, 0.1 g of the compound filled into tubes made of alkali-free Jenaglas, melted and sterilized at 102 ° C. for 3 hours.

Example.

Illustration of the gold-lung protein connection.

The ones described in Example! Procedure until the ninhydrin-free pulmonary protein compound is boiled through Pressed a fine sieve and floated up in a little water, then an excess of one ι percent gold chloride solution added, thoroughly stirred and a few hours ditched. The washed and dried until the wash water is free of gold Gold-lung protein compound is a reddish-gray powder that is found in water and the is insoluble in most organic solvents.

Examples. ^8s

Representation of the zinc - testicle protein connection. The one according to Example 5 until it does not appear the ninhydrin reaction boiled testicular protein compound is after pressing through suspended through a fine sieve in water and stirring with excess zinc sulfate solution offset and left standing for a long time. The resulting zinc-testicular protein compound is washed with water until the washing water shows no zinc reaction shows more and then finished in the usual way. It forms a light gray Powder, which contains the zinc in masked or non-ionized bond, and in Water and the common organic solvents is insoluble.

Example 9.

Representation of the cobalt protein compound from thyroid glands.

The thyroid glands are shredded in a known manner, bled and kept with them for a long time Boiled water until 2I of the cooking liquid is concentrated to ι ecm with 1 ecm of a percentage Ninhydrin solution no longer gives a reaction. You now add to that washed up with water Organ add a 10% cobalt nitrate solution in excess, leaves some Stand for hours, then wash with water until the excess cobalt ' is removed, dehydrated with acetone, dried in vacuo, pulverized, sieved and sterilized one hour at 105 ° C. The cobalt compound is a fine, gray powder, which in water and is insoluble in common organic solvents.

Claims (1)

Patent claim: Further development of the method for representation protected by patent 300513 of heavy metal compounds of protein substances, characterized in that heavy metal compounds or colloidal Heavy metals can act on such proteins from normal animal or human organs or tissues that using known methods to the extent of all water-soluble components, such as degradation products, are released that they no longer show any reaction with triketohydroindene hydrate (ninhydrin). .