DE2656675B1 - Antiserum fuer radioimmunologische Bestimmungen,seine Herstellung und Verwendung - Google Patents
Antiserum fuer radioimmunologische Bestimmungen,seine Herstellung und VerwendungInfo
- Publication number
- DE2656675B1 DE2656675B1 DE19762656675 DE2656675A DE2656675B1 DE 2656675 B1 DE2656675 B1 DE 2656675B1 DE 19762656675 DE19762656675 DE 19762656675 DE 2656675 A DE2656675 A DE 2656675A DE 2656675 B1 DE2656675 B1 DE 2656675B1
- Authority
- DE
- Germany
- Prior art keywords
- conjugate
- antiserum
- determination
- bsa
- test animals
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- NIKZPECGCSUSBV-HMAFJQTKSA-N testosterone 17-glucosiduronic acid Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(CCC(=O)C=C4CC3)C)CC[C@@]21C)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O NIKZPECGCSUSBV-HMAFJQTKSA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- 238000012360 testing method Methods 0.000 claims description 16
- 241001465754 Metazoa Species 0.000 claims description 14
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 9
- 210000002700 urine Anatomy 0.000 claims description 9
- 210000004369 blood Anatomy 0.000 claims description 8
- 239000008280 blood Substances 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
- 229940098773 bovine serum albumin Drugs 0.000 claims description 5
- 239000002504 physiological saline solution Substances 0.000 claims description 5
- 229960003604 testosterone Drugs 0.000 claims description 5
- 102000014914 Carrier Proteins Human genes 0.000 claims description 4
- 108010078791 Carrier Proteins Proteins 0.000 claims description 4
- 239000002671 adjuvant Substances 0.000 claims description 4
- 230000000890 antigenic effect Effects 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 claims description 3
- 150000003431 steroids Chemical class 0.000 claims description 3
- 210000001124 body fluid Anatomy 0.000 claims description 2
- 239000010839 body fluid Substances 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 230000000052 comparative effect Effects 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 230000003054 hormonal effect Effects 0.000 claims description 2
- 239000000427 antigen Substances 0.000 claims 3
- 102000036639 antigens Human genes 0.000 claims 3
- 108091007433 antigens Proteins 0.000 claims 3
- 239000007927 intramuscular injection Substances 0.000 claims 2
- 238000010255 intramuscular injection Methods 0.000 claims 2
- 238000003127 radioimmunoassay Methods 0.000 claims 2
- ONYFNWIHJBLQKE-ZETCQYMHSA-N N(6)-acetimidoyl-L-lysine Chemical compound CC(=N)NCCCC[C@H](N)C(O)=O ONYFNWIHJBLQKE-ZETCQYMHSA-N 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 239000000745 gonadal hormone Substances 0.000 claims 1
- 230000001900 immune effect Effects 0.000 claims 1
- 238000003018 immunoassay Methods 0.000 claims 1
- 230000001771 impaired effect Effects 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 230000035899 viability Effects 0.000 claims 1
- 230000037029 cross reaction Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 5
- 229930182480 glucuronide Natural products 0.000 description 4
- -1 steroid glucuronides Chemical class 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 230000009260 cross reactivity Effects 0.000 description 2
- 150000008134 glucuronides Chemical class 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- VFUIRAVTUVCQTF-UHFFFAOYSA-N (17-oxo-5alpha-androstan-3alpha-yl)-beta-D-glucuronic acid Natural products O=C1CCC2C1(C)CCC(C1(CC3)C)C2CCC1CC3OC1OC(C(O)=O)C(O)C(O)C1O VFUIRAVTUVCQTF-UHFFFAOYSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 206010020112 Hirsutism Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000012443 analytical study Methods 0.000 description 1
- VFUIRAVTUVCQTF-BSOWLZGZSA-N androsterone 3-glucosiduronic acid Chemical compound O([C@H]1C[C@@H]2CC[C@@H]3[C@@H]([C@]2(CC1)C)CC[C@]1([C@H]3CCC1=O)C)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O VFUIRAVTUVCQTF-BSOWLZGZSA-N 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002381 testicular Effects 0.000 description 1
- WAQBISPOEAOCOG-DYKIIFRCSA-N testosterone sulfate Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)OS(O)(=O)=O)[C@@H]4[C@@H]3CCC2=C1 WAQBISPOEAOCOG-DYKIIFRCSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19762656675 DE2656675B1 (de) | 1976-12-15 | 1976-12-15 | Antiserum fuer radioimmunologische Bestimmungen,seine Herstellung und Verwendung |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19762656675 DE2656675B1 (de) | 1976-12-15 | 1976-12-15 | Antiserum fuer radioimmunologische Bestimmungen,seine Herstellung und Verwendung |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2656675B1 true DE2656675B1 (de) | 1978-06-08 |
| DE2656675C2 DE2656675C2 (enExample) | 1979-02-08 |
Family
ID=5995534
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19762656675 Granted DE2656675B1 (de) | 1976-12-15 | 1976-12-15 | Antiserum fuer radioimmunologische Bestimmungen,seine Herstellung und Verwendung |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE2656675B1 (enExample) |
-
1976
- 1976-12-15 DE DE19762656675 patent/DE2656675B1/de active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE2656675C2 (enExample) | 1979-02-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8339 | Ceased/non-payment of the annual fee |