DE2413546A1 - Continuous colorimetric analysis - where sample and test reagent are heated above boiling pt in capillary tube - Google Patents

Continuous colorimetric analysis - where sample and test reagent are heated above boiling pt in capillary tube

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Publication number
DE2413546A1
DE2413546A1 DE2413546A DE2413546A DE2413546A1 DE 2413546 A1 DE2413546 A1 DE 2413546A1 DE 2413546 A DE2413546 A DE 2413546A DE 2413546 A DE2413546 A DE 2413546A DE 2413546 A1 DE2413546 A1 DE 2413546A1
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DE
Germany
Prior art keywords
capillary tube
analysis
continuous
test reagent
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE2413546A
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German (de)
Inventor
Dr Ir Simatupang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SIMATUPANG IR MARULI DR
Original Assignee
SIMATUPANG IR MARULI DR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by SIMATUPANG IR MARULI DR filed Critical SIMATUPANG IR MARULI DR
Priority to DE2413546A priority Critical patent/DE2413546A1/en
Publication of DE2413546A1 publication Critical patent/DE2413546A1/en
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Process for continuous colorimetric analysis at high temps. consists of pumping the continuously flowing liquid for analysis into a capillary tube together with the test reagent and heating to above the boiling pt. Subsequently, either before or after the colorimetric determination (M) it is cooled until no disturbing air bubbles are present. In chromatography, colourless outputs from a column can be detected by colorimetric methods with the aid of reagents to give coloured products. In general the effectiveness of such reactions is increased, the higher the temp. at which they are carried out. Methods currently available cannot exceed the boiling pt. of water. The method described permits temps. in excess of this to be used.

Description

Verfahren zur kontinuierlichen colorimetrischen Analyse bei höheren Temperaturen ============ Die Erfindung betrifft ein kontinuierliches Verfahren, wobei die Analysenlösung und das Nachweisreagenz bei höheren Teiperaturen bis über den Siedepunkt erhitzt und anschließend colorimetrisch bestimmt wird.Method for continuous colorimetric analysis at higher Temperatures ============ The invention relates to a continuous process the analysis solution and the detection reagent at higher temperatures up to over the boiling point is heated and then determined colorimetrically.

Bei der Sdulenchroiatographie farbloser Verbindungen wird zu de.In the column chromatography of colorless compounds, de.

Stiuleneluat kontinuierlich ein Nachweisreagenz zugegeben. Das Gewisch wird nun erhitzt um eine möglichst große Farbausbeute zu erzielen-und anschließend colorimetriert.A detection reagent is continuously added to the column eluate. The muddle is now heated in order to achieve the greatest possible color yield - and then colorimetrized.

Der Zweck der Erfindung liegt nun darin, mit Hilfe einfacher Mittel die Erhitzung des Gemisches, bestehend aus dem Sduleneluat und dem Nachweisreagenz, bis Uber den Siedepunkt zu ermöglichen ohne die anschließende colorimetrische Bestimmung zu beeinträchtigen.The purpose of the invention is now, with the help of simple means the heating of the mixture, consisting of the column eluate and the detection reagent, to allow above the boiling point without the subsequent colorimetric determination to affect.

Für die kontinuierliche colorimetrische Analyse farbloser Säuleneluate gibt es zwei Verfahren, nämlich die dynamisch analytische Technik (Prospekt der Firma Technicon GmbH, Frankfurt/Main) und die Teflonkapillarschlauchmethode (Prospekt der Firma LKB-Instruments MUnchen, Biotronik Wissenschaftliche Geräte,Frankfurt, und Cenco Instrumenten Mij N.V. Breda, Holland).For continuous colorimetric analysis of colorless column eluates there are two methods, namely the dynamic analytical technique (prospectus of Technicon GmbH, Frankfurt / Main) and the Teflon capillary tube method (prospectus of the company LKB-Instruments Munich, Biotronik Scientific Devices, Frankfurt, and Cenco Instruments Mij N.V. Breda, Holland).

Beide Methoden haben den Nachteil, daß die Reaktionszeiten ui eine gute Farbausbeute zu erhalten, verhältnismäßig lang sind, weil die Reaktionstemperatur nur bis zu Siedepunkt des Wassers eingestellt werden kann.Both methods have the disadvantage that the reaction times ui a To get good color yield are relatively long because of the reaction temperature can only be adjusted up to the boiling point of water.

Der Erfindung liegt die Aufgabe zu Grunde, die Reaktionstenperatur zu erhöhen und dadurch die Reaktionszeit zu verkuerzen, ohne die anschließende colorimetrische Bestimmung zu beeinträchtigen.The invention is based on the object, the reaction temperature to increase and thereby shorten the reaction time without the subsequent colorimetric Affect determination.

Diese Aufgabe wird erfindungsiäßig dadurch gelöst, daß das Reaktionsgemisch in den Kapillarschlauch bis Uber den Siedepunkt erhitzt und anschließend gekühlt wird.This object is achieved according to the invention in that the reaction mixture heated in the capillary tube to above boiling point and then cooled will.

Die mit der Erfindung erzielten Vorteile bestehen insbesondere darin, daß durch die kurze Reaktionszeit die Bandenverbreitung in dem Nachweissystem reduziert wird; dadurch ist es möglich, eine kürzere Trennsäule zu verwenden, wodurch sich die Trennzeit verringert.The advantages achieved with the invention are in particular: that the short reaction time reduces the band spread in the detection system will; this makes it possible to use a shorter separation column, which means the separation time is reduced.

Ein AusfUhrungsbeispiel der Erfindung ist in der Zeichnung dargestellt und wird im folgenden näher beschrieben.An exemplary embodiment of the invention is shown in the drawing and is described in more detail below.

Es zeigen: Figur 1: Schema des Analysensysteus.The figures show: FIG. 1: Scheme of the analysis system.

Figur 2: Ergebnis der chromatographischen Trennung Figur 3: Ergebnis einer ähnlichen Trennung it einem Autoanolyzer der Firma Technicon GmbH., Frankfurt/M. Figure 2: Result of the chromatographic separation Figure 3: Result a similar separation with an autoanolyzer from Technicon GmbH., Frankfurt / M.

Claims (2)

Patentanspruche: Verfahren zur kontinuierlich coloriuetrischen Analyse bei höheren Temperaturen, dadurch gekennzeichnet, daß die kontinuierlich fließende Analysenflussigkeit und das Nachweisreagenz in eine Kapillarschlauch bis Uber den Siedepunkt erhitzt und anschließend vor oder nach der kontinuierlichen colorimetrischen Bestiiiung gekühlt wird, bis keine störenden Luftblasen vorhanden sind.Claims: Process for continuous colorometric analysis at higher temperatures, characterized in that the continuously flowing Analysis liquid and the detection reagent in a capillary tube to over the Boiling point heated and then before or after the continuous colorimetric Determination is cooled until there are no disruptive air bubbles. Literatur: 1) Technicon GMbH., Frankfurt/M., The Tochnicon Sugar Chrouatography System.Literature: 1) Technicon GMbH., Frankfurt / M., The Tochnicon Sugar Chrouatography System. 2) LKB Instruments GmbH., MUnchen, Automatischer Aminosäuren-Analysator, Publicationsnummer Nr. 3201(A).T1.2) LKB Instruments GmbH., Munich, automatic amino acid analyzer, Publication number 3201 (A) .T1.
DE2413546A 1974-03-21 1974-03-21 Continuous colorimetric analysis - where sample and test reagent are heated above boiling pt in capillary tube Pending DE2413546A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE2413546A DE2413546A1 (en) 1974-03-21 1974-03-21 Continuous colorimetric analysis - where sample and test reagent are heated above boiling pt in capillary tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2413546A DE2413546A1 (en) 1974-03-21 1974-03-21 Continuous colorimetric analysis - where sample and test reagent are heated above boiling pt in capillary tube

Publications (1)

Publication Number Publication Date
DE2413546A1 true DE2413546A1 (en) 1975-10-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE2413546A Pending DE2413546A1 (en) 1974-03-21 1974-03-21 Continuous colorimetric analysis - where sample and test reagent are heated above boiling pt in capillary tube

Country Status (1)

Country Link
DE (1) DE2413546A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3641251A1 (en) * 1986-12-03 1988-06-09 Degussa METHOD AND APPARATUS FOR CONTINUOUS COLORIMETRIC DETERMINATION OF THE CYANIDE CONCENTRATION OF AQUEOUS SOLUTIONS

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3641251A1 (en) * 1986-12-03 1988-06-09 Degussa METHOD AND APPARATUS FOR CONTINUOUS COLORIMETRIC DETERMINATION OF THE CYANIDE CONCENTRATION OF AQUEOUS SOLUTIONS
US4871681A (en) * 1986-12-03 1989-10-03 Degussa Aktiengesellschaft Method for the colorimetric determination of the cyanide concentration of aqueous solutions

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