DE202014101208U1 - Diagnostic device for Helicobacter pylori - Google Patents

Abstract

A diagnostic device (10) for Helicobacter pylori, comprising: a main body (20) with an accommodation space (26); a reaction element (30) which is stored in the accommodation space (26); a pressing member (40) which is removably inserted in the accommodation space (26) and has a projection (42); a first spreading element (50) which is stored in the accommodation space (26) and is positioned between the reaction element (30) and the pressing element (40), the first spreading element (50) having a first surface (501), one to the first Surface (501) rear second surface (502) and an opening (52) through the first surface (501) and the second surface (502), wherein the projection (42) removably sits in the opening (52), the second Surface (502) has at least one liquid-conducting groove (54) extending from a peripheral wall (521) of the opening (52) to a peripheral wall (503) of the first expansion element (50); and a second spreading member (60) seated in the opening (52) and having a periphery (603) in contact with the peripheral wall (521) of the opening (52).

Description

BACKGROUND OF THE INVENTION

1. Technical area

The present invention relates to microbiological test devices, and more particularly to a diagnostic device for Helicobacter pylori.

2. Description of the Related Art

Helicobacter pylori, a species of Gram-negative spirilla, was identified in 1983 by Canadian physicians Barry J. Marshall and Robin Warren as the major cause of gastric diseases such as gastric ulcer, gastritis and gastric cancer. Helicobacter pylori has a particular helical structure and flagellum, and thus is able to move in the gastric mucus, attach to the surface of the gastric epithelial cells and secrete urease, to convert urea in the gastric mucus into ammonia, thus forming a protective shield to protect itself from digestion by the hydrochloric acid in gastric juice and thereby multiply in the stomach. Furthermore, Helicobacter pylori can excrete many degradative enzymes to damage the gastric mucosa and cause gastric epithelial cells to develop inflammation due to the loss of gastric mucosal protection, which in turn increases the risk of gastric ulcer, gastritis, and gastric cancer.

Diagnosis of Helicobacter pylori infections is mainly based on some invasive methods using endoscopes and some non-invasive methods. The known invasive methods include urease bioassay, pathological examination, microbiological culture and polymerase chain reaction (PCR). On the other hand, the usual non-invasive methods include serum antibody tests, C ¹³ / C ¹⁴ urea breath tests, stool or urine antigen test, and testing of salivary cultures. Of these, the urease bioassay has been widely adopted by many hospitals because it provides quick and easy results, and many commercially available test strips using this method have been introduced to the market.

A typical existing test strip using the urease bioassay mainly comprises a main body, a pressing member, a filter, a partition and a reaction member. In use, the collected stomach sample is placed on the filter and the pressing element expresses juice from the stomach sample. The juice passes through the filter and subdivision and reacts with the reaction element. In the event that the juice of the stomach sample contains urease, the reaction element changes color. The WO 2011/112106 , the US 2010/0028937 and the US 2012/0094371 Some are examples of prior art devices which use screens, holes or grooves formed on the partition to cause the juice to spread vertically or to traverse outer edges across the reaction element. However, these existing designs do not provide good liquid distribution and can cause problems associated with prolonged reaction time and delayed readout of the result.

SUMMARY OF THE INVENTION

In view of this, the first object of the present invention is to provide a diagnosis device for Helicobacter pylori which provides excellent liquid distribution.

In order to achieve the above object, the disclosed Helicobacter pylori diagnosing apparatus comprises a main body, a reaction member, a pressing member, a first spreading member, and a second spreading member. The main body has an accommodation space. The reaction element is deposited or stored or arranged in the accommodation space. The pressing element is removably stored in the accommodation space and has a projection. The first spreading element is deposited in the accommodation space and positioned between the reaction element and the pressing element. The first spreading member has a first surface, a second surface rearward from the first surface, and an opening passing through the first surface and the second surface. The projection is removably inserted into the opening. The second surface has at least one liquid-conducting groove extending from the peripheral wall or the peripheral wall of the opening to the peripheral wall of the first propagation element. The second spreading member is seated in the opening so that the periphery of the second spreading member is in contact with the peripheral wall of the opening.

With the above-mentioned technical features, the disclosed Helicobacter pylori diagnosing apparatus effectively shortens the distance to be traveled by the liquid and facilitates rapid distribution of the liquid, thereby shortening the reaction time and accelerating the reading of the result.

The detailed contents and features of the present invention are illustrated in the following sections. However, it should be understood by those skilled in the art that the detailed description and specific embodiments for practicing the present invention are intended for purposes of illustration only and are not intended to limit the scope of the present invention.

BRIEF DESCRIPTION OF THE FIGURES

The attached figures are provided for a better illustration of embodiments according to the invention, wherein:

1 Figure 3 is a perspective view of a Helicobacter pylori diagnostic device according to a preferred embodiment of the invention showing the cover raised;

2 is an exploded view of the diagnostic device for Helicobacter pylori;

3 Figure 3 is a perspective view of a first spreading element of the Helicobacter pylori diagnostic device;

4 a planar view of the first propagation element of 3 is; and

5 a cross-sectional view of the first propagation element along the line 5-5 of 4 is.

DETAILED DESCRIPTION OF THE INVENTION

The invention may best be understood by reference to the following detailed description of illustrative embodiments with the accompanying drawings.

As 1 and 2 show comprises a diagnostic device 10 for Helicobacter pylori according to the present invention mainly a main body 20 , a reaction element 30 , a pressing element 40 , a first propagation element 50 and a second propagation element 60 ,

The main body 20 is shaped as a standard test strip and includes a base 22 and a cover 24 , The cover 24 covers the upper surface 221 the base 22 removable or removable from. The base 22 has an accommodation room 26 and a ventilation duct 28 on, both from the upper surface 221 are lowered. The accommodation room 26 is essentially a round basin and has a round, from the bottom 261 ascending lead 262 on. The ventilation duct 28 is essentially an S-shaped groove, which coincides with the accommodation space 26 in contact. If the cover 24 the base 22 covered, is the accommodation room 26 through the ventilation duct 28 in contact with the environment.

The reaction element 30 is an annular piece, which is in the accommodation space 26 sitting. The reaction element 30 is loaded with chemicals such as urea and phenolsulfonephthalein. Comes the reaction element 30 In contact with a solution containing urease, the urease hydrolyzes the urea to produce ammonium and carbon dioxide. Consequently, the solution becomes alkaline and the reaction element 30 turns red.

The round plate shaped pressing element 40 has a raised section 42 on and sits removable in the accommodation room 26 , In particular, the pressing element 40 one to the cover 24 attached page 401 on, and the raised section 42 is at the back or opposite side 402 provided. If the cover 24 the base 22 covered, the pressing element sits 40 in the accommodation room 26 , Will the cover 24 lifted, the pressing element 40 from the accommodation room 26 away.

The first propagation element 50 is in the accommodation room 26 deposited or stored or arranged and is located between the reaction element 30 and the pressing element 40 , It is a round plate with a first surface 501 , one to the first surface 501 back second surface 502 and one through the first surface 501 and the second surface 502 continuous opening 52 , The opening 52 is located in the middle of the pressing element 40 so that the raised section 42 removable in the opening 52 sitting. If the cover 24 the base 22 covered, is the raised section 42 in the opening 52 fitted. Will the cover 24 raised, becomes the raised section 42 out of the opening 52 away. As 3 to 5 show, points the second surface 502 at least one liquid-conducting groove 54 on, extending from the perimeter wall 521 the opening 52 to the peripheral wall 50 of the first propagation element 503 extends. According to the present embodiment, four of these liquid-conducting grooves 54 on the second surface 502 of the first propagation element 50 formed and arranged radially as two opposite pairs. Each of the liquid-conducting grooves 54 has a sector shape. It should be noted that the number and shape of the liquid-conducting grooves 54 not limited to those disclosed in the embodiment.

It will open 1 and 2 Referenced. The second propagation element 60 is made of a porous material, such as cellulose paper, so that it can utilize capillary forces to facilitate liquid distribution. It has a sample-bearing surface 601 on and back to the sample bearing surface 601 an area 602 for color matching. The sample-bearing surface 601 serves to carry the stomach sample to be examined, and the area 602 for color matching has a comparative color to the discolored reaction element 30 in order to be able to determine the test result. The second propagation element 60 is slightly larger than or equal to the opening 52 with respect to the flat surface, leaving one in the opening 52 fitted second propagation element 60 the periphery 603 of the second propagation element 60 and the peripheral wall 521 the opening 52 be in contact with each other.

After a stomach sample on the second propagating element 60 is placed, the cover is 24 closed and the pressing element 40 is pressed to the raised section 42 to press against the stomach sample to extract the juice. The extracted juice then flows on the second spreading element 60 from its center to its periphery 603 , By guiding the liquid-conducting grooves 54 , the juice flows from the peripheral wall 521 the opening 52 laterally and outwardly, over the entire reaction element 30 to be distributed. Thereby, the disclosed configuration helps to shorten the distance traveled by the juice extracted from the stomach sample and accelerates its distribution, thereby reducing the reaction time and speeding up the reading of the result.

In addition, during the process, as long as the cover 24 to the base 22 is closed, the ventilation duct 28 on the one hand adequately some air from the accommodation space 26 on the other hand prevent the liquid in the accommodation space 26 evaporates too early, giving the test result for a longer time.

Finally, professionals may prefer that the number of fluid-conducting grooves 50 alternatively, it can be two and they are opposite each other on the second surface 502 are arranged (not shown). Alternatively, the second surface 502 with only one liquid-conducting groove 50 can be provided (not shown), and the desired liquid distribution can also be achieved.

The present invention has been described with reference to the preferred embodiments, it being understood that the embodiments are not intended to limit the scope of the present invention. Furthermore, since the contents disclosed herein may be readily understood and implemented by one of ordinary skill in the art, all equivalent substitutions or modifications that do not depart from the inventive concept are intended to be encompassed by the appended claims. BRIEF DESCRIPTION OF THE SEQUENCE DRAWINGS USED IN THE FIGURES Diagnostic device for Helicobacter pylori 10 main body 20 Base 22 upper surface 221 cover 24 accommodation space 26 ventilation duct 28 reaction member 30 press member 40 page 401 . 402 elevated section 42 first propagation element 50 first surface 501 second surface 502 opening 52 peripheral wall 521 Liquid-conducting groove 54 second propagation element 60 Sample carrying surface 601 Area for color matching 602 periphery 603

DEPLOYMENT OF BIOLOGICAL MATERIAL

Domesticated biological material [please provide the information in the following order: filing name and date and serial number of deposited deposit]: None

Biological material deposited abroad [please provide the information in the following order: country and name of institution of deposit and date and serial number of deposit provided]: None

QUOTES INCLUDE IN THE DESCRIPTION

This list of the documents listed by the applicant has been generated automatically and is included solely for the better information of the reader. The list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions.

Cited patent literature

• WO 2011/112106 [0004]
• US 2010/0028937 [0004]
• US 2012/0094371 [0004]

Claims (7)

Diagnostic device ( 10 ) for Helicobacter pylori, comprising: a main body ( 20 ) with an accommodation space ( 26 ); a reaction element ( 30 ), which in the accommodation room ( 26 ) is stored; a pressing element ( 40 ), which can be removed into the accommodation room ( 26 ) and has a lead ( 42 ) having; a first propagation element ( 50 ), which in the accommodation room ( 26 ) and between the reaction element ( 30 ) and the pressing element ( 40 ), the first propagation element ( 50 ) a first surface ( 501 ), one to the first surface ( 501 ) back second surface ( 502 ) and one through the first surface ( 501 ) and the second surface ( 502 ) continuous opening ( 52 ), wherein the projection ( 42 ) removable in the opening ( 52 ), the second surface ( 502 ) at least one liquid-conducting groove ( 54 ) extending from a peripheral wall ( 521 ) of the opening ( 52 ) to a peripheral wall ( 503 ) of the first propagation element ( 50 ) extends; and a second propagation element ( 60 ), which in the opening ( 52 ) and a periphery ( 603 ), which with the peripheral wall ( 521 ) of the opening ( 52 ) is in contact. Diagnostic device ( 10 ) for Helicobacter pylori according to claim 1, wherein the main body ( 20 ) One Base ( 22 ) and a cover ( 24 ) comprising a top surface ( 221 ) the base ( 22 ) removably covered, and wherein the accommodation space ( 26 ) from the upper surface ( 221 ) the base ( 22 ) is lowered. Diagnostic device ( 10 ) for Helicobacter pylori according to claim 2, wherein the base ( 22 ) an S-shaped ventilation duct ( 54 ) extending from the upper surface ( 221 ) and with the accommodation space ( 26 ). Diagnostic device ( 10 ) for Helicobacter pylori according to claim 1, wherein the at least one liquid-conducting groove ( 54 ) two of the liquid-conducting grooves ( 54 ) lying opposite one another on the second surface ( 502 ) are arranged. Diagnostic device ( 10 ) for Helicobacter pylori according to claim 1, wherein the at least one liquid-conducting groove ( 54 ) four of the liquid-conducting grooves ( 54 ) which radially on the second surface ( 502 ) are arranged. Diagnostic device ( 10 ) for Helicobacter pylori according to claim 1, wherein the liquid-conducting groove ( 54 ) has a sector shape. Diagnostic device ( 10 ) for Helicobacter pylori according to claim 1, wherein the second propagation element ( 60 ) has an area slightly larger than or equal to an area of the opening ( 52 ).

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