DE2013799C3 - Arrangement for hydrodynamic focusing of a flow of a particle suspension into the measuring opening of a particle analyzer - Google Patents

Description

5. Arrangement according to claim 4, characterized in that the invention is based on the object indicates that a venting device 40 to avoid disadvantages and an arrangement of the (11,12) is provided. To create the type mentioned at the beginning, in which the

6. Arrangement according to claim 2, characterized in that the particle suspension passes through the path indicates that the measuring opening (3) is arbitrarily selected in a measuring opening and as such is accurate Reaction glass-shaped container (30) is provided, can be determined.

and the feed tube (20 ') on the outer 45. According to the invention, this is achieved in that side of the container (30) is arranged. the outlet opening of the feed tube under

7. Arrangement according to one of claims 1 at any angle to the axis of the measuring opening to 6, characterized in that on both sides arranged at such a distance from the measuring opening the measuring opening (3) according to the Coulter- net is that the envelope flow is a continuous transverse principle one electrode (25, 31; 26) each is provided 50 narrowing of the cut of the current filament of the particle tail. causes suspension from the outlet opening of the feed tube to the measuring opening.

The continuous narrowing of the particle suspen-

sion through the sheath flow prevents sedimentation of particles and a spread of the particle flow after exiting the outlet opening of the

The invention relates to an arrangement for hydro-supply pipe. One can Eilamish in this way Focusing a stream of one side will cause all particles to move along one side suspension in the measuring opening of a particle an- a very specific passage path through the measuring device go through it by means of a sheath flow of particles and, on the other hand, the position of these ier liquid with one in the space in front of the measuring passage path within the measuring opening exactly., however not in the immediate vicinity before voting. So the envelope current exercises on the particle users, arranged supply tube for the pension over the entire area of the outlet "pension. opening of the feed tube up to the measuring

Such arrangements are known in / Gore η, »Improving Resolution in Coulter the flow conditions at the outlet opening unting by Hydrodynamic Focusing ", Journal of the feeding tube occurring and by the Uoid and Interface Science 26, 175 to 182 different parameters (specific gravity of

j ... ³ 4

Ä ^; article suspension, outflow rate, FIG. 5 a is a front view of a second embodiment

Äiv.'ysw.) Effects caused by the known arrangement example of the invention,

| Some remarks on hydrodynamic focus calculation still refer to Fig. 5b, a side view of the exemplary embodiment

^ s?% lead to satisfactory results, although the game according to F i g. 5 a,

The principle of hydrodynamic focusing is shown in FIG is used to the passage of the pension between the feed tube and the ^ Pfgartikelsuspension through the measuring opening along the measuring opening in the area in front of the measuring opening. fifiiliner to reach very narrow passageway. At On one for an attitude towards one

The centering ring 1 provided to the north of the outlet opening of the feed microscope is a small tube in which, according to the invention, the base plate! arranged, in which a measuring opening3 fpH:; 5the distance from the measuring opening is also provided. The diameter of the measuring opening 3 flpSist that also in comparison to the known approach is, for example, 100μ. On the base plate, a storage container 4 i || SSpension, which is closed with a plug 4 α , can be accommodated without the change in the desired flow rate being attached to the particle-free liquid SfftS its change in the desired flow velocity, i.e. during measurement the volume distribution according to SgPlialtnisse occurs in the space in front of the measuring opening. serves the Coulter principle of the electrolyte. He is

^if§S:; In the arrangement according to the invention can via a supply 5 with a channel 6 in the ff * 'is further from the connected in the known arrangements Grundplatte2 (see. Fig. 2). The liquid Ä (Spielman / Goren, op. Cit .; Thom / Harape / keit can thus from the storage container4 into the room i Sauerbrey.Z.ges.Ex.Med. 151,331 to 349 (1969); 20 17 above (according to Fig. 2, 3) the measuring opening 3, see also the older suggestion according to German Offen- langen. After passing through the measuring opening 3 used, the liquid then arrives in a likewise S-order of supply tube and measuring opening in the base plate! provided channel? which go off via I, so as to enable optical observation of the particles passing through the measuring opening through a connection piece 8 with the suction container 9 1, e.g. B. is connected at 25 (see FIG. 4). The suction container 9 is provided with fluorescence and absorption measurements without obstructing the view through the feed tube 10 with an opening 1 embedded in a stopper 9 α , to which a vacuum source is connected. Can be closed by shifting the feed. If, with the tube closed opposite the measuring opening, the storage container 4 can be precisely applied to the opening 10, a negative pressure I passage path of the particle suspension, then the liquid is provided from the front and through the measuring opening with the storage container 4 viewed with a microscope 3 to be brought into agreement in the Ab-S point. The suction container 9 is sucked. The space below the measuring % selection of an opening 3 lying on the edge of the measuring opening can also be vented by a vent line 12 which can be closed with a plug 11C to determine certain form factors for non-round parts. _{A a}

The measuring opening is formed by _u a _u in

"Turn the opening and therefore insert special pulse shapes an opening 13 of a plate 14 a clock stone

- result, the peculiarities of which is left with a passage. The plate 14 lies on one in the

- tap at the edge of the measuring opening easier to determine base plate incorporated paragraph 16, so that between

- are <° see the upper surface of the plate 14 and the opposite known arrangements (Thorn / upper edge of the base plate 2, the space 17 for the ^;; Hanfpe / Sauerbrey, Z. g ^es · Exp. Med. 151, 331 supply of the liquid from the channel 6zutM ^ _

to 349 (1969); see also the older suggestion, opening 3 remains on the upper surface of the

J measured German Offenlegungsschrift 1806512), from baseplate 2 a glass plate "18" is glued

■ an arrangement of the outlet opening of the feeder 45 whose upper surface (Fig. 3) is ground This

■ SJröhrchens directly from the measuring opening, glass plate 18 is with a »^» "f" ^^ ie at a distance in the order of magnitude of the large enough to assume a displacement of J * ² ^ diameter of the measuring opening, results in rungsröhrchens 20 within. ^ "gfw ^un sdnen Bern Further, the advantage that due to the much higher ches opposite the Meßoffnung3 not Denin Ouerschnittsverengung of the particle stream thread. ag- 50 the." giömemtsbedingte ⁸ double passages leading to an on the glass flat surfaces "^^ | 1 ^ ° ϊ £ lead to a falsification of the measurement result, as well as the plate 21, which is due to a (not shown) ^ ruciaeaer SuS difficulties, due to the printing against the G ^ plate 18. D e unnecessary precise processing and coaxial adjustment - The lower surface of the glass plate 21 is omitted just as the feed tube is sheared. Advantageous 55 fen, so that when printing on ^ s G ^ plate "further developments of the invention are in the lower 18 a sealing against the glass plate 21 ent

iS are exemplary embodiments of ^ The glass plate 21 is on the Ρ '«« ^ η «and

^{on the drawings} sssÄ

on a first version, ^ ". Ä ^ ™ ^ a cross-section along the line 11-11 in« ^ _r ^^^ ™ enlarged representation of the area a ₅ ^^^

Cut along the line IV-W ^ 23 ^ unden ^ er * - »

The height of the container 23 can be slid on a rod 24 when the particles pass through attached. The height shift is used for the nenen impulses. The risk of incorrect measurements that Change in the discharge pressure of the particle susceptibility can be attributed to the fact that with a full board The particles emerge from the outlet opening 22 of the feed at various points on the measuring tube 20 in the room 17. 5 opening 3 different pulse shapes occur,

By shifting the glass plate 21 opposite, this concentration of the particle flow

the base plate 2 can now be the entry point at which the inside of the measuring opening is switched off. In addition

the particle suspen-

sion in the flow from the reservoir 4 Chens 20 in the area in front of the measuring opening the

goes to the suction container 9, are introduced, the passage point of the particle flow is precisely determined

can be changed compared to the measuring opening 3. will.

In Fig. 6 the flow conditions are shown. 1 arrangement is the po-

represents. The outlet opening 22 is opposite the sensitive electrode 25 in the storage container 4, the geer-

On the axis of the measuring opening 3, negative electrode 26 in the suction container 9, which is considerably laterally displaced.

ben. 6 shows three possible positions of the assigned 15.

guide tube 20; two of them are in dashed lines. Absorption measurements are above the measured values Lines shown. The cross section of the flow of the opening 3 an objective 27 of a microscope pre-particle suspension see from the outlet opening 22 (see FIG. F i g. 2, 3). Below the measuring opening is of the feed tube 20 exits and at this a (not shown) radiation source, for example in the form The exit point is initially the cross section of the opening 20 of a condenser. The invention-22 corresponds, decreases in the flow of the particles moderate arrangement offers the advantage that the optical on the measuring opening 3 out considerably. That is an axis of the microscope exactly on that point in a row the increasing concentration of the stream can be adjusted within the measuring opening 3, mungsfeldes on the measuring opening 3, which passes from the through which the particle suspension and Course of the flow lines in the various 35 that on the other hand with a given position of the lens The positions of the inlet tube 20 can be clearly seen 27 relative to the measuring opening 3 by displacement becomes cash. Regardless of the position of the channel 6 of the feed tube 20 in the area in front of the measuring device Measuring opening 3 they form in the vicinity of opening 3 an additional setting of the through-first radially out and then go to the channel 6 trajectory of the particle flow within the Meßöffzu. As long as the feed tube 20 can be reached into the flow 3 without the exit flow field Opening 22 arranged in the area of laminar flow, through which the particles enter the flow network, which comes from the pressure difference between the forefield and obstructs the beam path. The through advice container 4 and suction container 9 and the pressure the arrangement according to the invention achieved low in the second storage container 23, as well as from the con-cross section of the flow of the particle suspension Structural design of the space 17 depends, 35 is allowed by the measuring opening 3, the orifice plate In this way it is achieved that the cross section of the microscope has a particle flow precisely on this passage path when passing through the measuring opening, so that disturbing secondary light largely nuiig3 is considerably smaller than its cross-section is switched off.

upon entry through the outlet tube 20 into FIG. 5 a and 5 b show a simplified, for

Flow between storage container 4 and suction 40 purposes of measuring the volume distribution of

container 9. Particles suitable embodiment of the invention

When measuring the volume distribution of the partial arrangement. The measuring opening 3 'is not disgusting According to the Coulter principle, the area of a test tube-shaped container 30 is used of two electrodes required, which are provided in the dated. On the outside of the container 30 is displaceable storage container 4 to the suction container 9 flowing 45 bar a storage container 23 'is arranged. From the pre-liquid, which then has to be an electrolyte, a tube 22a 'leads to a frozen storage tank 23' det will generate a current flow. Entry tube 20 '. Here, too, is the location of the

If a particle entry opening 22 'passes through the measuring opening 3 opposite the measuring opening 3'

through, so there is a current displacement and shiftable. The volume distribution measurement is now

a corresponding increase in resistance, which at 50 the entire container 30 in a further container

impressed current is used between the two electrodes. An electrode 31 is located in the

as a voltage pulse between the electrodes- container 30, the other electrode is outside the-

can be taken and a measure of the volume ses container arranged (not shown), so that too

of the penetrated particle. The volume here is a current flow through the measuring opening 3

The amount is then determined by corresponding stands

In addition 6 sheets of drawings

Claims (4)

[1968]). According to this, an improvement in the resolution of the measurement results is to be achieved in that all particles pass through the measurement opening on the same 1. Arrangement for hydrodynamic focus ben pass through passage. Experiments have shown, however, that a flow of a particle suspension in 5 shows that, with the specified measurement opening of a particle analyzer, the dimensioning ratios, which provide that by means of a sheath flow of particle-free fluid, the distance of the feed tube from the measuring substance, with a space in front of the measuring opening, tion is 33 to 165 times, preferably 65 to but not in the immediate vicinity of this, 130 times the diameter of the measuring opening, the supply tube arranged for the Susio cannot be reached with certainty. This is a pension, characterized in that this choice of the outlet does not exclude the outlet opening (22) of the feed stand that between the feed tube (20) at any angle to the guide tube and the measuring opening there is initially an axis of the measuring opening ( 3) is arranged in such a significant expansion from the feed tube to the measuring opening (3) that the 15 small exiting particle stream filament enters, which forms a continuous cross-sectional flow of a sedimentation of a part of the particles that can no longer be neglected of the stream filament of the particle suspension out of the sheath through the sheath flow from the outlet opening (22) of the feed and thus to a falsifying tube (20) up to the measuring opening (3). can lead to improvement of the measurement results and it 2. Arrangement according to claim 1, characterized GE 20 also brings with it that even if the indicates that the feed tube (20) ensures passage of all particles on a path the space (17) in front of the measuring opening (3) could be steadied, this passage path is arranged inside (18, 21) and lockable. do not precisely define half of the measuring opening itself 3. Arrangement according to claim 1 or 2, so that Jie evaluation in turn of this characterized in that the measuring opening (3) depends on the path and falsifies it for these reasons is embedded with a base plate (2) and that can be. This means that the ones for the input feed are also available the particle-free liquid in the so-called, known arrangement known-space (17) in front of the measuring opening (3) from a measurement result (a. A. Ο., P. 177, Fig. 2, p. Storage container (4) and that the partial 178, Fig. 3) in accordance with the test measurements of particle-free liquid from the other side of the 30 particles of known properties a considerable Measurement opening (3) via a suction container Falsification of the expected result (9,110) is sucked off. show. The known arrangement also assumes this 4. Arrangement according to claim 3, characterized in that in the event of non-compliance with the aforementioned indicates that the feed tube (20) dimensioning specifications for the distance from is embedded in a plate (21), which has a further 35 feed tubes and measuring openings one of the space (17) in front of the measurement opening (3) deterioration of the measurement results occurs (a. a. the recess in the base plate (2) O., p. 178); this should already be mentioned from the same is. th reasons be the case.