DE1916515A1 - Micropipette holder - Google Patents

Description

Beon, Dickinson and Company, East Rutherford, N.J., U «S.A. Micropipette holder

Many medical and technical research and processing work Technicians often have to move small, precisely measured amounts of liquid from one vessel to another or Bring a carrier material. This task is generally solved by the fact that the liquid in the required slope by suction or capillary action in a thin tube or sucked into a pipette and then into the collecting container or on the carrier material is emptied. These liquids are often considered to be radioactive, pathogenic, toxic, or contaminated and can therefore become dangerous if they come into contact with people or equipment the tubes or pipettes are thrown away after use, which also eliminates the need to wash them out.

The invention is based on the object of a reusable Holder for pipettes and capillary tubes xu aohaffen, with which a pipette grasped, held, filled, emptied and can be ejected after emptying, so that the user never comes into contact with the pipette and its contents comes.

The micropipette holder according to the invention is recognized by a soblay linder that looks from a front part and a rear part attached to it a displaceable, sealingly guided through the rear part and from outside takes up an actuating rod, the length of which corresponds approximately to the length of the hollow cylinder, and the one with its interior in communication vent opening and at its front end has an opening whose Diameter is slightly smaller than the inner diameter of the front part and into which an elastic plug is inserted 1st, which is manually inserted by a pipette holding a pipette in

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• Slidable in · di ··· releasing position iät- UtMi eiae «For receiving the pipette, serving through-hole aiifweiat, whose Durohaesser oonMltrmi ·· slightly larger iet all the outer diameter of the pipette. Furthermore iat in de « Hollow cylinder an ejector connected to the stopper provided »the an» it de «front end dar forward · g · - • A raised stanza with a sussaon-like back and a forked föralgee Has front part, the Durcbaesoer dia Back part · · slightly smaller than the inside diameter dea hollow cylinders at this point and the distance between the Prongs dea gave "l-shaped" front parts a apart from each other is larger ala the diameter of the pipette.

The vena of the stopper in its released position is "a pipette ia inserted through the LMafabearung of the stopaela" can be grounded. By moving the stopper, the pipette becomes feet-free. Dvreli Veraaliiaban der Staate und entaprechendea Freilebea or Abaperrea ά · τ XatlttftungaBffava «: can in the · Hohlsyliader and daaiit ia the pipette, the eaväaeentan pressure ratios are created» by the · pipette bit, also know the danger of ulceration, so that there is no ulceration tlberfttliuaf of the pipette and a resulting oversight rate of the salters is reduced. .

The Stipael iat vovaugaweise "with a vigorous Flanaoh 1 · Iaaeren and ait aiaaaj vertieren Flaaeob. auOertawlb dea leblayliaalere verseaa «» 4t * isiaaallf anr limit dea Yereeaiefcevegee daa Stffaaala bil4ea «ad die susaaaMn a · BlaaebaOrang limit, vtleaa aaae) a de« e “front flanach eiaen Aaaaoaebnltte ) U "inner outer diameter - aaer ala de" diameter of the opening receiving the plug so that the hole receiving the pipette is narrowed if the section of the larger outer diameter is generally the bore where the pipette is held,

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BADORfGlNAL

while in the other end division the pipette is freely open «Can be grounded or falls out.

The plug and ejector can be one piece or separate parts.

The front part of the hollow cylinder is advantageously transparent, Among other things, you can watch the filling and emptying of the pipette. This boobao attentiveness has been improved become »if at least one prong of the ejector is transparent and is designed as a magnifying lens or contains one. Alternatively, the hollow cylinder can be used in the area the prongs should be provided with old optical magnifying tools.

Further details and features of the invention emerge from the following description in conjunction with the drawings, in which an exemplary embodiment is shown. It shows

Pig 1 a side view of a pipette holder according to the invention,

Fig. 2 is a longitudinal section along line 2-2 in Pig. 1 in enlarged « Scale,

Pig * 3 shows a longitudinal section through the lower end of the pipette shark on an enlarged scale,

FIG. K shows a section along line kh in FIG. 3, and FIG

5 shows a partial section through the lower end of the pipette holder.

The pipette holder 10 consists of a hollow cylinder 12 which is composed of a front part 1 * »and a rear part 16, which are connected to one another in an airtight manner by a tendon connection 18. The front part consists of transparent tk ") material such as GLAE or a plastic, such as acrylic resin, can see into which the user in the interior of the HohlzjrlInders. The rear part 16 is concentric for ease of handling

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provided with parallel ribs Zk , the rear part 16 of the hollow shaft end 12 has a ventilation opening 26 which is connected to the inside of the planer cylinder 12.

The Endabsohnltte 20 and 22 de · Hohlsylindere 12 β iod mit Openings, which with the inside * »dee hollow cylinder · stay in contact. Duroh dee rear end 22 extends • I have airtight stern 28, which have their free ends outside de · Hohlaylindere 12 ait a * actuation · handle 3Ö ▼ • r * eb «n is. Di · Stenge 28 1st in the »Hollow cylinder 12 / rer- ' • otai «bb« r and na «in · scnrerlM ** ige sealing the same bu reach, know seals svleohen der Stenge 28 and de « Sndebeohnitt 22 may be provided. These seals are allowed to use the Do not impair the free longitudinal mobility of the rod 28. The rear part 16 of the pipette heater 10 can beiepieelwelee New polyethylene is made, which is sufficiently elastic, but the longitudinal movements of the rod 28 are susceptible the necessary airtightness at the same time Ensure rod 28 and de * hollow cylinder 12 su.

At the front end 20 of the hollow cylinder 12, an opening 35 is provided into which a plug 32 is inserted so that it can be moved in length, the part inside and the part outside of the hollow cylinder 12. Dmr Stttpeel $ 2 is elongated and has a Planeoh 36 bsw at each of its ends. 38, between which there is an undercoat, which has two sections ^ O and HZ *, which have different diameters. The plug 32 can consist of any "elastomeric, natural" or silicone rubber. The churned-up material must be sufficiently elastic if it can be deforalized with little force.

The front surface 36 of the support 32 is considerably larger than the opening 35, the end cut 20 of the Hohlayllnder 12 «or lies on the outside of the hollow cylinder 12, so that it is serves as a stop that prevents the Stepeel 32 from being able to

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Inner · d ·· Bohl cylinder 12 to be pushed. The diameter d ·· rear flange 38 let also larger · 1 · the diameter of the opening 35 »sodaO the flange the Stopper 32 prevents it from completely coming out of the hollow cylinder 12 to be pushed out »especially * if a Pipette 50 is ejected from the plug 32.

As can be seen from FIG. Z , the normal diameter of the front section kO of the constriction of the plug 32 is greater than the diameter of the opening 35 »while the diameter of the rear section kZ of the constriction is smaller than the diameter of the opening 35», in particular from Fig. 3 can be seen. Through the plug 32 a bore extending bk whose diameter is slightly greater than can be pushed hinduroh than the outer diameter of the pipette 50 so that dieee without difficulty through the bore hh 'when the plug 32 in the in Tig. 2 is the position shown. The front and rear end sections k6 and kB of the bore kk are slightly enlarged in terms of the durometer in order to facilitate the insertion of the pipette 50.

Within the limits “which are determined by the front and rear flanges 36 and 38, respectively, the plug 32 can be displaced in the longitudinal direction relative to the hollow cylinder 12, with a slight force being necessary to overcome the resistance caused by the slightly enlarged diameter of the section kO when forcing the section through the opening 35 *

For use, a pipette 50 or a capillary tube is inserted into the bore kk while the stopper 32 is in its front position shown in FIG. Since the diameter of the bore kk is somewhat larger than the outer diameter of the pipette 50, it can easily be pushed into the stopper. Then the whole plug 32 is in

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The baiter 10 is pushed in so that the front section kO of the constriction of the plug lies tightly in the opening 35, as shown. 3 «riohtlb iet. In order for the plug 32 to be able to move into this position, a deformation must take place and this deformation causes a narrowing of the bore kk in the area of the section kO _t where the pipette is held 50 feet. In the rearward position of the plug 32, the vend of the opening 35 presses on the plug, whereby the diameter of the bore kh is reduced, while in the front position of the plug the diameter of the bore hk is larger. The plug 32 can be moved from its front to a rearward position by exerting pressure on the front surface of the front flange.

When the pipette is held in the holder 10 in the manner described, the user can use the pipette holder to suck any desired amount of liquid into the pipette. In the case of pipettes that fill completely through capillary action, the user only needs to dip the tip of the pipette into the vessel with the liquid to fill the pipette. For pipettes that do not fill by capillary action, suction must be applied to fill the pipette. The rod 28 is used for this purpose. The user dips the front end of the pipette into the liquid and push the rod 28 with open vent 26 12 into the hollow cylinder, since the vent iet open 28, the leak in this insertion of the rod 28, displaced air through the vent Z6 and the liquid is not influenced. The user then utters the vent opening 26 and at the same time pulls the rod 28 outward on the handle 30. This creates a partial vacuum inside the hollow cylinder 12 through the rich liquid in the pipette 50

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tuft w £ rd. The rod 28 is pulled out as far as 4Vt * »« d | · Fill pipette 50 su. To extract <rym * i ** 9i.fpk * fm mV *** pipette 50, the reverse tsyaang durobgefüart, ie, the user sees length 28 fet! off · «·« SntlUftuagsloch 26 something from the front end H «hXaylind« r · 12 surttek and then he closes the discharge

The bottom of the rod 28 is in the Hohlaylinder (It states what a pressure is generated in the fiohlayl loader 12 The liquid is pressed in from the pipette 50. Because the contents of the pipette "it" in the collecting vessel • The "It" in · "carrier material, for example absorbent paper When the touch is brought, the liquid begins to flow out the plp «tt · au flow. If ee desired, the pipette 50 To eject its contents, the rod 28 becomes without locking The ventilation opening 26 is pushed straight into the hollow cylinder 12.

Veam dl · 1st liquid ejected, dl · pipette can warden ejected and the holder 10 can with a new pipette used w "gestures" Since the ejection of spent pipettes facilitate sw, 1st "la ejector provided within the Jk Hohlsrllndsrs 12th The ejector century is la weeentlichen sylindrlsch uad has a fixed rear section 52 and • inen gatelfttralgon front Absohnitt 58. The Einken 5 ^ «ad 56 d ·· front ·" Abeohnittee 58 have a very long distance from each other, that «ia · la d« n Sttfpael J & «ingeeohoben Plp« tt · old dl · ·· «alaht la Is ¹ Marung k-at. The outer duroha «ss« r d ·· behind · «section ·· 3 & let kl« ln «r as the inner« ar «aa * e« «rd ·· H« alerllad «rs 12« a. at this point, aa da · ▼ * t% * rb «level VUl, # a« ad empty the pipette 50 «! • did au '» e «lntrS« «tig« a. Dar Aasworfer j / k brews neither Um 8 «1β« · 1 Vt n * oh aa άτ Stan «· 28 attached aa β, send he can lie freely la Bcttlarllnder 12. The rüokw & rtlge d ·· Aa «w« 4pf «r · jk w«! Et «in« concave · recess aaf, in

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which the front end of the rod 28 can engage and the front ends of the prongs $ h and 5 *> lie on the rear flange 38 of the stopper JZ . In order to eject a pipette 50, the user only needs to press the handle 30 down while holding the hollow cylinder 12. The force is transmitted through the rod 28 to the thrower 34 and from there to the plug 32, whereby the plug 32 is pushed outwards, the bore 44 expanding in the manner described above.

The convex surface 60 of the cylindrical wall of the front part 58 of the ejector 34 acts as a magnifying lens in order to enlarge the part of the pipette 50 which lies in the region of the ejector 34, as shown in FIG. This makes it easier to fill the »pipette and reduces the risk of overfilling. -

The rod 28 should be so long that its front end touches the rear flange 34 when it is completely inserted into the hollow cylinder 12 and the plug 32 is in its pushed-out position according to FIG. 1 and 2 is. Others measured the length of the rod 28 to be so short that the stopper 32 of the rod 28 cannot be pushed completely out of the cylinder 12.

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ORIGINAL BATH 909844/1223

Claims (9)

Patent sayings Micropipette holder for laboratory work, approved <./ through a hollow cylinder (i2), dor sioh from a front feil (i4) and odnem attached to the rear Part (i6) composed and a displaceable, sealingly guided through the rear part (i6) and from auasen to be actuated rod (28) receives, the length of which corresponds approximately to the length of the hollow cylinder (12), and the one vent opening (26) communicating with its interior and an opening at its front end (20) (35), whose Durohmesser is slightly smaller than the inner diameter of the front part (i4) and in an elastic stopper (32) is inserted, which is manually moved from a pipette holding a pipette into a releasing one Position is displaceable and has a through bore (44) serving to accommodate a pipette, whose diameter is usually slightly larger than the outer diameter of the pipette, and one at the plug (32) adjoining the displaceable ejector (34), the one with the front end of the forward pushed rod (28) cooperating back part (52) and a fork-shaped front part (58), wherein the The diameter of the back part (52) is slightly smaller than the inner diameter of the hollow cylinder (12) on this Place and the distance between the prongs (54,56) of the fork-shaped front part (53) from each other is greater than that Diameter of the pipette. 2. Micropipette holder according to claim 1, characterized gekexinzeiohrnet, that the plug (32) with a rear flange (38) inside and a front flange (36) outside of the hollow cylinder (12), which together «limit the constriction next to the front flange (36) a section (4o) with a larger outer diameter and next to it a section (42) with a smaller one 9 0 9 8 4 4/1223 BATH ORIQINAL Outside diameter as the diameter of the opening (35) having, the outer diameter of the rear Flange (38) smaller than the inner diameter of the hollow cylinder (12), but larger than the diameter the opening (35) is. 3 * micropipette holder according to claim 1 or 2, characterized in that that the stopper (32) and the ejector consist of one piece. 4. Micropipette holder according to claim 1 or 2, characterized in that that the plug (32) and the ejector (34) are separate parts. . 5. Micropipette holder according to one of Claims T to h, characterized in that the lower part (i4) of the hollow eyeliner (12) is transparent. 6. Micrepipate holder according to one of the A _n SpXiICbO 1 to 6, because it can be said that the "indest""zinc" (5 ^ "56) of the ejector" (3%) is transparent and has an outer convex "surface (βθ), di «« 1 · enlargement line works. 7 * micropipette holder according to one of claims 1 to 6, characterized in that the hollow cylinder (12) in the Area of the prongs (5 ^ »56) of the Ausverfers (3 * 0 prefokussiarta having optical magnification means. BAD ORJGlNAL 909844/1223