DE1909059A1 - Device for quantitative thin-film measurement - Google Patents

Description

r- Dr. Hugo Wllcken 2 |, Feb. 1969

! pt.-Ing. Thomas Wilcken
Patent Attorneys 1 Q C] QQ ζ Q

24 LCbeck, Breite Strasse 52-54
Telephone 75888

Applicant:

Sohoeffel Instrument-Corp., Westwood, NJ * USA, Bookerstr. 2k

Device for quantitative thin-film measurement

The invention relates to an optical density measuring device for substances of a translucent or semi-translucent nature in liquid, semi-liquid or solid form, which substances are found in particular in the field of thin-layer, paper, gelatin and disk chromatography. Since the. Base plates for thin-layer imaging consist of a glass plate which is coated with an adsorbent, and since these coatings are applied by means of elongated spray devices, a certain percentage of waviness can be found in the adsorption substances applied. This waviness is of a quality which becomes noticeable in the case of changes in transmission of up to BU 25 % in the basic adsorbent. Similar transmission errors occur when the adsorbents are applied by spray or immersion processes. Paper strips made of normal filter paper or other papers that are used for chromatography also contain differences in size or inequalities in terms of the changing fiber diameter. Daselbf meets for Gelatlaeplattan su, v «l« h «either duroh QieAen o4i * sad · -

0-

r method are obtained,

0a unstable medium, which · as an adsorbent for

0OÄd3 # / 1O28 - 2

various chemicals and substances (one feesehi "ie" Ben is) is used for optical density or TranenUsionemessungen of adsorbed or migrated chemicals © sub punch accurately useful reproduce tmi> adsorbent the len zero display absolutely uniform, In order to measure media that have migrated or are adsorbed in spots as a reliable basis or baseline, an additional substance orundadaorptlonemitfel This object is to be carried out with the present invention described below.

The above-mentioned elsctro-optl-ifäie measuring device forms a train * and a sample beam bsw "-beam bundles, rad swar both beams have an extra" small distance apart "- which ensures that there is a difference between the sample and the Besugsstrahl on similar or almost similar adsorbent locomotives in the basic adnorptioifBittel. hit, even ₉ when the plate is uneven in its surface state. The resulting ratio serves as a straight baseline and is enlarged when it is recorded "The evaluability of the chromatography strip (s)" A cutting device for dividing the thin-layer adsorbent into strips, which serve as sample and reference strips and white the sample "and the fly flies are brought into extreme -NShe to one another, is provided in order to make the light beam separation and the streak separation identical.

When the sample and reference beams have the same permeability result, the by means of a suitable photoelectric V © r- _ifclltnii «with the read DurehlflaslfucaitsKrad dos sample and

of the traction strip lead to a ratio of "one". This "one" ratio is set equal to 100 % transmission or optical * density from the value equal to zero. The mathematical relationship is sufficient for the following equations:

PC 1

T-. «; D «Ig -;

^T R ^T

1
¹⁸ Tu * * ~ * Tp ^ T ₀

In the equations:
·: T * transmission without transmission, Ty '* transmission ratio,
TU * Transnisslonsunterohied,
D β dense «(optical);

the indices R ■ Besugskanal,

P * sample channel with no separate chemical,

C · separate chemical.
This is how the difference in weight looks like

When a dfinne pushes an adsorbent or another Adsorption media by means of a cutting device or the like separate the body in strips, kiss white strips with im Cross-section, contours are used for measuring. Pure the saehgememe.working of this system are the substances, who are to be measured separately, single! Loh on the Applied specimen strips, and the specimen · and the Becug · etreifen are developed in development media. Only

SOS839 / 1 02S bad original .-- *

the added and separated sample is measured by means of the ratio scanner or reader measured, and the effect of the Orundadsorptlonsmittel and the solvent is zero considered when the closely adjacent reference strip is the same Thickness than the sample strip and the solder mean flow can be considered the same in both strips.

The above-mentioned device requires a light source with high intensity in the entire spectral range, as is the case with light sources of the open or ßeschJoseenen arc type, such as is found in carbon-arc, xenon, xenon-mercury lighting units or other light sources. The emitted radiation of the light source is filtered dur <& h optisch® filter or a monochromator, and the selected monochroftatlsehe energy is adjusted eiaf a passend® SohlltsOffnung · The spectral radiation, 'dl' from this Sdh lit "opening occurs _r a in the direction of · Doppe lech Ut »opening eert to both ewei LichtsurahietibOttdel" 4 * *** "the same, spectral characteristic and fihnllehe AuseohnittiibiaeesttA§e? I. as well as maintaining the same intensity. The substance that is to be eaten becomes in this "special 9all of a thin-sighted or other" 3aj? Omtitogra "& e in twei gloieh broad"; Strip divided, and "was by means of an entry; - or device, which contains individual spring-loaded" Binrite pin cutting knives, which produce closely adjacent and dimensionally repeatable eye and sample surfaces of the greatest possible similarity. These incised or cut strips are guided into the beam path by means of guides

909839/1028 -

the closely adjacent bundles of light rays with similar spectral Characteristics and intensities. If this bundle of light rays is aligned in the longitudinal direction and only are separated by a small light deflector plate is a double-beam system with a groove spacing between the nitol point of the Heß- or sample beam bundle originated; and with regard to the dense NShG these two bundles of rays can a single monochromator output slot or filter output slot appropriately enlarged and divided into two equal parts in length.

A mechanical component is provided in order to be able to set both the width of the slit with regard to the illumination by means of the reference and the probe as well as the length of the slit of these two bundles of rays, either on the same side, or individually. The light that emerges from this beam opening will run semi-parallel. Immediately below the two closely adjoining openings, a Botor-driven guide device is attached, which enables the user of the appropriate device to hold the insole plates and samples in a suitable manner. This guide or feeding device does not interfere with the light beam and the linear movement of the device can be synchronized with a card recorder or other visual inspection. Below eer TChrmnfseinrlohtung, in which the under investigation bsw. Examining sample is attached, swel input channels are in deti reader bsw. Scanner of the Dot Action Absohnlttee f— tmU% wmtfs oreehen. This swel KaAUe »lad with d * reto-

translucent windows made of a suitable material and know to be replaced by interference wedge filters which can be set to do just that Appearance of predetermined areas of spectral BNs in the De » section to be bearable.

The detector section contains one or more compartments or other suitable radiation detectors. Since the samples under test usually have accumulated lightnesses, the detectors are attached at an appropriate distance in order to detect a monitored zone of integrated energy. This car park is new in that it contains an old type of scanner or reader, detection devices such as photomultipliers, do not generally see any linear response characteristics over a? Evidence flee voi *. There are therefore aach arrangements made _a USN artificial diffusers in the Increase in brightness & ngskan & l & sweet of the detector "section ·" aneuo ^ dunes, which scatter from the sample koHQi ₉ in all directions, herbairufOhren to "ine suitable Integr & tl & ss for quantitative Messurgen un light.

In the attached drawing, an execution code of the previously described with the system. In d # r Hung represents L semi-parallel bundles of light rays Wavelength or widen tioht, which by a lh For "a double mouse execution restricted let and where" With the Doppelkellaus execution la direction de · arrows · A is movable, among other things the Belwaehtungeeehlltsiange su ateoern. Dtr Illumination is further restricted by the plat- »

809839/1028 - T

th 2 and 3, which are attached in such a way that they can be moved in the direction of the arrows "B", so that the sledge division can be carried out uniformly. The bundles of light rays which pass through these openings in the plate impinge on the pre-cut adsorbent strip of the thin layer k or another chromatographic material. One of these strips represents the sample strip, the other the reference strip, and both strips are pointed by spring-loaded insertion pins 7 and by the branching movement in the direction of arrow C during the preparatory table formation for the examination. This incision or incision device can be removed from the device and operated separately therefrom, if so desired. The scanning movement C also serves * in the indifferent transport of the sample and the bed through the light beam

The gestreut® radiation which ven the ehroaia ^ ogiraphiechen sample and siiistritt the Besugsatrelfee is "the detectors 6 dicroh Öffsfsmigeaü 9 for the optically" gaeaitdt electrical signal conversion, * detected by the deflectors 5 mid ε. The refraction 8 in this scheme is a length below the sample area, this refraction aaifc filters or? movable interference trowel 10 stir Velle length control can be equipped ·

Although the above is only an epesial exercise requirement ben 1st, 1st the invention, however, is not limited to this, Ve can s «B. by -yach people making obvious changes- »En without deviating from the inventive concept.

909839/1026 BAD original

Claims (1)

*. "! £ 09059 Dr. Hugo Wlfcken 9 \ Dipl.-Ing. Thomas Wilcken '* Patent attorneys M Lübeck, Breite Strasse 52-54
. Remote call 7 S3 68 Applicant: Sohoeffel Instrument-Corp., Westwood, N.J. USA, Book «rotr. 2Ί Claims l.j device for quantitative thin-film measurement, characterized in, daft the execution of a double-beam examination system for thin-layer plates or thin-layer Contains medium, the system with attachable and detachable arrangements sum scratching or pre-cutting of thin-layer media in a width maft for precise pesting the optical beam continuation is provided, in particular with regard to the half-length for these rays to produce an illumination of the specimen and infestation strelfs as completely as possible to provide and, among other things, to grasp the medium present on one of the two strips as completely as possible and a mathematical one To form ratio, the strips are provided with the special feature that the centers of the The reference and sample jets are closer to each other in order to avoid the impact on a similar or almost similar adsorbent dicice to secure on the thin film plate, which in turn is transported by a movable slide is used to ensure an accurate optical reading and a continuous Provide a relationship between the sample and the agent, which are made by photoelectric or other facilities is used to quickly display sample material . 909839/1028 ■ ^ ■ '■ ^ ■ - ■ _mD ORIGINAL to allow the sample strip when no sample material is on is present on the strip, even if the adsorption center is thick on the plate is gradually decreasing or the plate has other inconsistencies, which is normal for an uneven base lines that are switched off by the new feature. ^2. Device according to claim 1, characterized in that it is equipped with components both for controlling the slot width and the Sehlltzlfinge of the closely adjoining sample and reference strips and for controlling the entry line width of the sample and reference strips. 3 · Device according to claim 1, characterized in that the Sampler or reader with a single monochromator from «? one who goes out ch Uta is armed of such a form has, which has the plate lighting double slot with Lieht, defined spectral bandwidth. 4. Apparatus according to claim 1, characterized in that the device with structural features sum adjustable fastening one or more single or double interference wedge fil- . ter in the closely adjoining entrance chambers of the Abletdetektorkammer is equipped to the selected strip width or to determine the position, including the wavelength continuous band control run individually or in conjunction with one of the two or with both channels at the same tent su can. 5. Apparatus according to claim 1, characterized in that the daft 909839/1028 · - ' BAD ORlQJNAt Output channels of the sample and the Bexugslleittstrahl one or the same side from the optical illumination area of the The sample can be raised or lowered in an adjustable manner, so that the sample and Besugsstrelfen with sieh UndeuM®? Compensate for thickness. 6. Gate direction according to claim 1, characterized in that the lighting position that the output channels for the samples * · and Contains the Besugsstrahl, is attached so that it is from the » Find out the investigation area and re-insert it exactly can be warden, if so desired. 7. The device according to claim 1, characterized gekarunzeiahnet, daft the Blnrit »- or Binsohneldeinrichtung tui generating the descriptive and the probation, which the complaints etc. Sample beam width compensates, can be started on the device and decreases & ar is arranged, davit them by hand or by; other · Vorkahan the device can be operated. ORIGINAL BATH 909839/1028