DE1717111C3 - Mixture of feed to accelerate the weight gain of livestock - Google Patents

Description

4) Color: colorless powder
5) Color reaction:

reagent Result

The invention relates to a feed mixture that ϊο accelerates the weight gain of livestock.

In recent years it has been shown that antibiotics, e.g. B. Aureomycin, when fed to livestock have the effect of accelerating the animals' weight gain. Although the suitability of aureomyein was known for the purpose mentioned, new substances were sought, with which one could still achieved faster weight gain and the purpose in terms of feed conversion more effective with lower cost and without undesirable side effects can be achieved.

The invention is a feed mixture for accelerating the growth of livestock, consisting of a mixture of cattle feed and enduracidin or its acid addition salts dispersed therein in amounts of 5 to 50 mg / kg forage.

In an advantageous embodiment of the invention, the feed mixture contains the enduracidin as the hydrochloride.

Enduracidin, a metabolite of microorganisms, has the structure of a polypeptide. Its "physical and chemical properties are given below. It is made from the culture broth of Streptomyces fungicidicus registered in the American Type Culture Collection under numbers 21013 and 21014 is deposited, won.

Unless otherwise stated, the term "enduracidin" used here refers to the free base and / or the harmless salts with acids, e.g. B. the hydrochloride to understand. Enduracidin has it like that the following physicochemical properties:

Ninhydrin

positive (pale brownish violet)

Barton's reagent positive (pale blue)

(Mixture of the same room

share 1% aqueous

Ferric chloride and

1% aqueous potassium

ferricyanide)

Alkaline potassium discoloration takes place

permanganate (reduction)

6) Melting point:

205 to 225 ° C (sintering), 225 to 240 ⁰ C (decomp.)

7) solubility:

Enduracidin is easy in pyridine, dimethylformamide, dilute hydrochloric acid, aqueous alkaline solution soluble (with decomposition) in aqueous methanol, aqueous ethanol, aqueous acetopn and aqueous Butanol soluble but insoluble in water, absolute ethanol, pure butanol and pure acetone or difficult to dissolve.

8) Rf value (paper chromatography):

Measured using the ascending method on "Whatman No. 1" filter paper (W. and R. Baiston Ltd., Great Britain).

solvent

Rf value *)

1) Elemental analysis:

Enduracidin consists of C, H, N, O and Cl atoms and still contains 3.36 ± 0.5% chlorine atoms in its molecule.

2) Specific rotation:

[«]» = + 85 ° ± 10 ° (C = 0.5, in dimethylformamide)

3) absorption spectrum:

λ 95% MeOH

Max
λ η / 10 HCl

Max
λ η / 10 HCl

EJi ± 15th 230 πιμ 220 ± 10 263 πιμ 150 ± 10 230 ηιμ 195 ± 10 272 πιμ 112 ± 10 250mü 335

Acetic acid / n-butanol / water (1: 4: 5) 0.45 ± 0.1 n-butanol, saturated with water 0.0

Pyridine / n-butanol / water (3: 4; 7) 0.80 ± 0.1

*) The substance is easily adsorbed on filter paper under neutral conditions.

Enduracidin can be in pure or raw form, »: B. as a culture broth of a microorganism, the enduracidin forms (for example Streptomyces fungicidicus ATCC 21013 or 21014), as an extract of this culture broth or one can be set in the form of its acid addition salts.

By using enduracidin according to the invention, it is possible to achieve surprising results in the breeding of meat-producing cattle

aim. The results are better than using an antibiotic such as aureomycin. To this Surprising results include, for example, a faster weight gain and a higher one Efficiency in terms of feed utilization. These results lead to improved rearing of livestock producing fish such as chickens, laying animals, roast chickens, pigs, geese, cows, Ducks and turkeys.

The amount of enduracidin in the feed is in the range from 5 to 50 mg per kg of feed. the The amount of feed mixture according to the invention to be fed can be determined in the usual way be done by a specialist.

The feed can of course also contain antibiotics such as aureomycin, mikamycin, oleandomycin, penicillin, tetracycline, amino acids such as glutamic acid, aspartic acid, leucine, lysine, tryptophan, valine, serine, proline, glucine, alanine, isoleucine, phenylalanine, arginone, methionine or their Contains salts, vitamins such as vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C, vitamin D, biotin, folic acid, vitamin K, vitamin E, vitamin P, inositol, orotic acid, alpha-lipoic acid, etc.

Acute toxicity: The mean lethal dose (LD ₅₀ ) of enduracidin in mice is 880 mg / kg body

weight when administered intraperitoneally and at 66 mg / kg body weight when administered intravenously. In rats, the mean lethal dose of enduracidin is 300 mg / kg body weight when administered intravenously Administration.

The invention is further illustrated by the following comparative experiments and examples

Attempt 1

ίο 400 chicks (Goto-Hiiku No. 3), which were 3 days old and weighed about 60 g each, were divided into four groups of 50 male and 50 female animals. Two groups, hereinafter referred to as test groups, received 22.2 mg of enduracidin per kg of feed. The other two groups, hereinafter referred to as comparison groups, received 22.2 mg aureomycin per kg feed. Each group was raised by so-called soil feeding and could eat as they please. From the beginning up to an age of 30 days, the feed of the composition mentioned in Example 1 (starter feed for broiler chickens) and then the feed of the composition also mentioned in Example 1 (broiler final feed) were given to all groups. The results are given in Table 1.

Table 1

Age, days
10

groups
Ver test
same

20th

Ver test
same

Ver test
same

40

Ver test
same

Ver test
same

60

Ver test
same

100 100 100 100 100 99.0 100 99.0 100 99.0 99.0 161 354 375 635 649 926 942 1140 1205 1522 1613 110.3 100 105.9 100 102.2 100 101.7 100 105.7 100 105.9

Growth rate,% 100

Body weight, g 146

Growth 100

coefficient,% *)

Feed conversion **) 2.13 2.12 1.95 1.80 2.01 1.96 2.39 2.29 2.62 2.42 2.70 2.52

♦) Ratio of the weight gain of the test animals to the weight gain of the comparison animals.
♦ *) Feed weight in kg, which was necessary for the increase in body weight by 1 kg.

It can be seen from Table 1 that the test group showed a weight gain of 5 to 11% and a 0.15 better feed utilization compared to the comparison animals, these values being valid for the age of 10 and 20 days, respectively. At the age of 60 days, the test group showed an average body weight of about 5% higher than the comparison group and 0.18 better feed conversion than the comparison group.

Attempt 2

400 chicks (Goto-Hiiku No. 3), which were 3 days old and weighed 60 g each, became divided into four equal groups of 50 female and 50 male animals each. Two groups, hereinafter referred to as test groups, received 15 mg Enduracidin per kg feed of the same composition as in experiment 1. The other two Groups, hereinafter referred to as control groups, received 15 mg aureomycin per kg feed the same composition. Each group was fed by what is known as soil feeding reared at will. The results are after listed in Table 2.

From Table 2, it can be seen that the test group had a 6 to 9% increase in weight and a showed about 0.1 better feed conversion than the comparison group at the age of 10 and 20 days.

Furthermore, the test group at the age of 60 days showed a mean body weight of 4.8% higher and 0.15 better feed conversion than the comparison group.

5 Days 100 20th 17th 17 1 1 1 40 ( test r 50 test 60 test groups
Ver test
same 158 Ver
same Ver
same 100 I. Ver
same 100 Ver
resembled 100 Table 2 Old,
10 100 108.9 100 100 I. 951 99 1218 99 1585 145 1.66 364 30th 928 102.5 1163 104.7 1512 104.8 100 100 test Ver
same test 100 2.41 100 2.60 100 2.74 Breeding rate,% 1.76 1.92 100 100 100 2.48 2.72 2.89 Body weight, g 386 604 616 Growth
coefficient; ent,% *) 106.0 100 102.0 Feed conversion * *) 1.83 2.21 2.16

*) Ratio of the weight gain of the test animals to the weight gain of the comparison animals. **) Feed weight in kg, which was necessary for the increase in body weight by 1 kg.

Example 1 Roast Chicken - Feed Mixture

component

Corn 54.85 56.65 Soybean meal (45%) 16.00 14.00 wheat flour 5.00 5.00 Fish meal (60%) 10.00 8.00 fish solubles 3.00 3.00 Alfalfa flour 3.00 3.00 Sebum (30%) 6.00 8.00 Calcium phosphates 1.60 1.60 Table salt 0.25 0.40 Lipophilic vitamin premix 0.05 0.05 (containing A, D and E) Vitamin B premix (contain 0.15 0.15 tend B ₁ , B ₁₈ , B ₆ and Bi ₂ ) Mineral premix (commercial
common)
2.2% enduracidin premix - 0.05 (dispersed in lactose) 0.10 0.10

Example 2 Chick feed mix

Starter final feed (% by weight) (% by weight)

Components starter In front feed starter feed (G) (G)

All in all

100.00 100.00

Corn Soybean Flour (44%) Wheat Alfalfa Flour Fish meal (60%) Dried whey Meat and bone waste (50%) Condensed fish solubles Dried solubles from distillation residues. Calcium phosphates. Ground mussel shells or Lime Salt Manganese Sulphate Flavors 10% enduracidin premix (dispersed in lactose)

600 1000 560 600 600 60 20th 50 100 50 50 50 60 50 30th 20th 30th 30th 10 10 0.5 0.5 0.5 1 0.2 0.5

Example 3 Starter feed for broiler chickens

Components Weight (G) Corn 1050 Soybean meal (50%) 625 Alfalfa flour 50 Poultry by-product meal 50 Dried whey 50 fat 90 Calcium phosphate 30th Ground mussel shells or lime 35 Manganese sulfate 0.5 Flavors 0.5 10% enduracidin mixture 0.2 (dispersed in lactose)

Example 4 Final foot for roast chicken

i s ρ i e 1 7

Pig feed

Components (B) 5 Components Gram Corn 700 Corn 1340 wheat 600 Wheat intermediate product 300 Soybean meal (50%) 320 Soybean meal (44%) 200 Poultry by-product meal 100 IO Meat meal 60 Dried soluble ingredients of Alfaifa flour 60 Distillation residues 50 Calcium phosphate 10 Condensed fish solubles 50 Ground clams or lime 20th Alfalfa flour 60 salt 10 fat 50 1 K Manganese sulfate 0.25 Calcium phosphate 30th ¹ O 10% enduracidin premix 0.65 Ground mussel shells or lime 30th (dispersed in lactose) salt 10 Manganese sulfate 0.5 Flavor 0.5 30th Example 8 10% enduracidin mixture 0.2 Starter feed for calves (dispersed in lactose) ß p i <«η i p 1 S Components Gram U ν 1 3 U 1 ν 1 * J
Piglet milk substitute 25th Corn 560 Wheat Middle Product 400 Soybean meal (44%) 400 Components Starter feed Flaxseed meal 300 (G) Dried soluble ingredients of 50 30th Distillation residues Cornmeal 30th Alfalfa flour 100 wheat flour 28 Condensed fish solubles 50 Soybean meal (treated at 120 ⁰ C) 25th molasses 100 Fish meal 8th Ground mussel shells or lime 30th molasses 4.5 35 salt 10 10% enduracidin premix 0.05 Manganese sulfate 0.25 (dispersed in lactose) Sodium glutamate 0.5 NaCl 0.3 10% enduracidin premix 0.3 Precipitated calcium carbonate 0.4 (dispersed in lactose) Secondary calcium phosphate 0.6 40 2.2% aureomycin premix 0.2 (dispersed in soybean cake) Example 9 pepsin 0.1 Seasoning for pigs 0.05 Growth feed for ducks Vitamin B ₁₂ (2.2%) 0.05 45 Components Gram Sodium cyclohexyl sulfamate 0.09 Example 6 Corn 500 Piglet pre-starter feed wheat 764 5o Soybean meal (44%)
Alfaifamohl 60 Components Gram

Yellow corn flour sucrose skimmed milk powder fish meal (60%) soybean meal (44%) Dried whey Condensed fish solubles fat

Calcium phosphate

Ground clam shells or lime salt, iodized manganese sulfate

10% enduracidin premix (dispersed in lactose) sodium glutamate

435 Wheat middle product 200

200 fish meal (60%) 50

600 meat and bone waste (50%) 100

100 55 Ground oats 10

400 Lactose-free whey 10

100 ground mussel shells or lime 40

40 salt 5.5

gO manganese sulfate 0.5

15 S ₀ 10% enduracidin premix 0.5

10 (dispersed in lactose)

10 sodium glutamate 2 0.25

0.5 The invention can be exploited by law 65 borrowed provisions, in particular by the feed

1 Mittelgesetz, limited.

Claims (2)

■ ') Claims: 1. Feed mixture to accelerate the growth of much), consisting of a mixture of cattle feed and enduracidin dispersed therein or its acid addition salts in Amounts from 5 to 50 mg / kg forage. 2. Feed mixture according to claim 1, characterized in that it is the enduracidin Contains hypochlorite. Most important infrared absorption bands in μ (KBr method): 3.05 (strong)
3.25 shoulder)
3.38 (shoulder)
5.75 (shoulder)
5.95 (shoulder)
6.09 (strong)
6.24 (shoulder)
6.47 (shoulder)
6.55 (shoulder)
6.61 (strong) 6.90 (middle!) 7.23 (medium) 7.63 (medium) 8.10 (wide, strong) 8.51 (medium) 9.90 (medium) 10.40 (weak) 11.92 (medium) 12.30 (weak) 12.75 (very weak)