DE1632405A1 - Mixing method and device - Google Patents

Description

In the field of biology and chemistry, analytical methods are used in which a liquid density gradient is brought about} this happens e.g. with the ultracentrifuge analysis as well as with the electrophoresis © This Gradient is brought about with the help of two liquids, from which one has a higher specific gravity than the other; the two liquids are in different proportional quantities mixed together »to ensure the sealing © des to vary the resulting mixture · Is perfect mixing required in this area of work? and there are difficulties * such a durßhmisctong summon! their.

Alas typical "known method! Rakes i @ r liquids is to use a container? & @ G> old one is EüJmrorrielitussg sided _v tor the dee Holds? From using a
are spinning germ ₉ which awlß @ h®E © ines

009833/1803

source and the actual stirring device is arranged. One of the liquids initially fills the container, and the contents of this container are slowly drawn in, while the second liquid from a second container into the first Container is transferred, so that the specific gravity of the liquid in the first container and thus also the specific gravity of the liquid withdrawn from this container continuously increased or decreased. Although it can A sufficient mixing of the liquid can be achieved, but it is necessary for the apparatus to be movable Provide component that is located in the liquid, as well as a drive power source.

The invention now provides a method for producing a substantially homogeneous mixture of flowable Substances in which turbulence is generated in the substances will, j to mix the fabrics; be for this purpose the substances passed through a channel at the same time, and the substances are deflected so that they are in each other. rotate alternating sections of the channel in opposite directions.

lera © r, the invention provides a mixing device, which a Bautsil rafaStg äu & a channel open at both ends off3? © nsü5 ₁ as well as components that hinder the flow of the material © du: ijü Sen Kaaal, these components compared to dam®s ? wätat®n component arranged stationary and aica la iai'cieinaaäQj? afeweehselenden sections of each channel

lichtes. length- helical lines extend.

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In one embodiment of the invention, the components mentioned in the second place fill an outer zone, their Cross-sectional area corresponds to about eight subunits of the channel door section; however, this cross-sectional area can also "up to about can be reduced to half the cross-section of the canal

Appropriately changes the Drehriohtung by the Channel flowing substances at least eight times.

Sine applicability of the method and the device according to the invention is particularly in the mixing of liquids for the production of a density gradient column as it is used ζ · Β · in centrifugal analysis »

Further details and advantages of the invention emerge from the following description of an exemplary embodiment on the basis of the drawings.

Mg. 1 is a schematic illustration of a device for generating a density gradient.

Fig. 2 shows in longitudinal section a part of a device according to Pig. 1 mixing device used.

Mg. 5a and 5b show two embodiments of one mixing device according to the invention

The desired effect of the device is brought about by the fact that two liquids which are miscible with one another, one of which has a density which is equal to or lower than the lowest required density, and of

009833/1603

to whom the other has a density »which is higher than that highest required density or equal to the required density, simultaneously pumped through a mixing chamber and closed be conveyed to a container in which the liquid column is to be generated. The flow rate of at least one of the liquids is varied so that the desired density gradient is achieved. A common liquid is a strong sucrose solution that can be mixed with water in any proportions, but they are stirred to a considerable extent must if it is to form a homogeneous mixture with water; this is especially true when the relationship between the Sucrose solution and water has a high value.

In the device according to FIG. 1, a concentrated sucrose solution 1 is conveyed through a branch piece 3 with the aid of a peristaltic pump 2, and water 4 is conveyed through the same branch piece with the aid of a peristaltic pump 5. The liquids are therefore simultaneously conducted to a container 7 via a mixing device 6. The desired density gradient is brought about in the container 7 by slowly increasing the flow rate of the sucrose solution by means of suitable regulation of the pump 2. An uptake of air is essentially prevented by the fact that the device is only flowed through at a low speed, for example at a speed of about 100 our / min, and by the fact that the device is completely filled with liquid before the liquid reaches the container 7 is delivered.

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The mixing device 6 comprises a tube 9 »which has an inner diameter of, for example, 3 mm and a length of 150 mm and contains a set of, for example, 15 open wire screws 10, with every second wire screw 11 having a certain G-arrangement, while the one between the wire screws 11 lying wire screws 12 have the opposite thread direction. In one embodiment, the wire diameter is approximately one-third the inside diameter of the pipe and the pitch of the screw threads is approximately twice the wire diameter. It has been shown that with this arrangement and at a flow rate of about 100 owr / mln an essentially homogeneous mixture is obtained. If you are working with flow velocities that differ significantly from this, other dimensions will probably prove to be more expedient.

The mixing device has a double effect. First, the helical wires cause the outer layers of the liquid flow are set in rotation and slowed down, so that a turbulence arises. Second the changes in the direction of rotation cause additional turbulence.

The mixing device can in the manner described Loose Brahtsßhrswiien contained alternatively one can use the

Screws & u des? Attach Xanenwand d © e Eötes © des · ®i @ anefeiMsm »For example WnuQU ät® Qahv & nh®n bxw Mqtall odsy fülee Isgeteüeag ise old ierWaefl © ia lGOJS 4g $ _c Bßo l © te Kai die Setoaafeem W

consist of any suitable material, e.g. metal, Plastics, glass or quartz. If the overall dimensions of the mixing device are to be reduced, one can Bend the pipe yourself, even into a spiral or screw, as shown in Flg. 3A and 3b respectively.

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Claims (12)

BA! 0Eli2! AIS PROBLEMS 1. Process for producing a substantially homogeneous S-emisehes made of flowable materials, whereby a turbulence is generated in the materials to ensure thorough mixing to effect, characterized in that the materials are passed simultaneously through a channel (9), and that the materials are caused to be in the channel © ine perform a rotating movement, this rotating movement in each second section (11) of the channel in a certain direction takes place while the turning movement in the separation centers between these sections (12) of the channel in the opposite sighting 2. The method according Ansprneli H ₅ in g l © £ © © NNS leimet., That the materials of a rectilinear Strönrangsweg be deflected so as to extend along flow paths of the channel sehraiabenlinienförmiger Move @ iÄ in the outer part ₀ 3 · Method according to claim 1 or 2 ₉ äaüMroh characterized in that the direction of rotation of the flow is changed mindeeteas eight times » 4 · Mixing device to carry out the Aasprueh method. 1 "to 3 ₉ characterized _{9 that the} flow path in the channel (9) is an obstacle that is formed by the channel or sections (10) which are fixed in place and are located. 009833/1603 extend along nasty channel, these components or sections in every second section (11) have a certain G angle, while the components or sections in the intermediate sections (12) of the channel have the opposite direction of flight, and wherein the energy, through which a rotating movement of the materials is caused, comes exclusively from the energy that the Materials is communicated. 5. Mixing device according to claim 4, characterized in that that the mentioned components or sections (10) prevent the flow of materials in the outer region of the channel (9) and that there is an obstacle-free flow path which extends through the central part of the channel extends. 6. Mixing device according to claim 5, characterized in that the mentioned components (10) on the inner wall of the Channel are attached. Y. Mixing device according to claim 4 to 6, characterized in that the components or sections mentioned are designed as helically wound components (10) which are arranged one behind the other along the channel (9) 8. Mixing device according to claim 5, characterized in that the sections are formed on the inner wall of the channel or are related to it. 9 · mixing apparatus according to claim 4 to 8, characterized in that the sections (10) as obstacles a 009833/1603 Occupy a cross-sectional area not larger than one Half of the cross-sectional area of the channel (9) * 10. Mixing device according to claim 9, characterized in that that the sections (10) occupy an area as indentations, about eight ninths of the channel cross-section is equivalent to. 11 · mixing apparatus according to claim 4 "to 10, characterized in that _t check the channel along a spiral or ssfe & mieiiliiiiösfcraiigeB, train, extends (Fig. 3a and 3b) · 12. Method to aacfc, claim 1 "to 3 or device according to claim 4 to 11, characterized in that the The method or the device serves to produce a density gradient column (Fig. 1) » The patent attorney 009833/1603 Le e rs eιίe