DE1617769A1 - Process for the production of milk containing specific antibodies and preparations made therefrom - Google Patents

Description

BIPL-INQiH-J ₄ HUBNER,

PATENT LAWYER »Ο 77769

■ ί

896 Ketnpfen, l, call 23Z9T

Uncfieuer 84a-, 3a

Az. T P 16 $ 7 769 * 8-41 1 β, April 1970

SrJSberhard Rehm, Dietmannsr ± ed K / m - H 3/4

Br.Eberhard R ehο, 8961 Dietmannsriedj Vockenthal 1

Method for obtaining specific Ischöü antibodies containing milk and preparations made therefrom.

The invention relates to a process for the recovery of specif laugh antibody-containing milk produced and preparation _»r from a dairy animal riden antigens are supplied deai at" a wait time is inserted and the animal old antibodies fortified milk is taken out.

Such a method is known (5st8rr.Patentachr.ift Vt * ZiZ 9Tf) * According to this known method, one should be given two to eight weeks before the feeding animals are thrown

Munich 151919, "DeuUAe Bsnk Kemplen 10 /« J10 *

Solution or suspension of the antigens through the teat canal introduced into the udder. The treatment is repeated at regular intervals «The first removal of Antibody-containing milk takes place after throwing "It takes two to eight weeks from the first antigen application, until the first milk that has been enriched with antibodies can be started.

There is another method with the above measures known (brit, patent 98S 175), in which the animal Antigens at least in four increasing doses weekly Intervals are administered, so that here, too, a period of time between antigen injections and antibody removal is at least four weeks "

In contrast, the essence of the invention is that milk withdrawal is started for the first time within a period (of the order of magnitude) of twelve or twenty-four hours after the first antigen application, that further withdrawals are made until the titer has dropped. Another feature of the invention is that the withdrawn milk is broken down into a lipoprotein phase. and a skimmed milk phase and the further recovery of antibody-containing substance from the first phase follows. The invention also consists in the use of the method according to the invention for increasing the value of foodstuffs, in which the antibody-containing substance is added to these foodstuffs. 109814/2019

So far, would in the FächweIt assumed * that is the antibody titer must determine nation in milk by Messen'des titer in Bluteerum means Bakterieti-Agglut! _O Only after Poeltlvwerden of Serumtitere with AntikSrpermilch-Eutnahme begonnenö A corresponding fiter in serum occurs only after a week at the earliest, usually not until after two "to animal weeks after the first antigen application, namely through recognizable agglutination of homologous bacteria. For this reason, the two well-known procedures also follow the doctrine that about four weeks before the first antibody removal The antigens are to be applied. According to experience, this period of time is sufficient for a measurable! pus-by means of bacteria-agglutination ira blood serum su srreielieu.

According to the invention, it has now been found that the development in the blood serum and that in the milk never run synchronously. The maximum antibody titer in the blood serum is considerably later than the maximum titer in the milk a similar course of the antibody titer over time, namely a steep increase up to the maximum titer with a subsequent gradual decrease, but there is a considerable time difference in both developments. While the maximum antibody age in the blood serum is two to four weeks after the antigens have been applied for the first time, the corresponding antibody titer in the milk is nice after twelve to thirty hours.? In ₀

f 098 14/2 0 19 SADOF

The invention has considerable advantages. Within a very short time, for example within twenty-four hours, specific antibodies can be obtained according to the invention for the immediate therapy of sudden accident situations (tetanus or the like) for immunization purposes, for allergy therapy, for acute transplant purposes or transplant preparation. These completely new possible uses have so far been ruled out because it has been accepted as a fact that the earliest generation of antibodies is possible after about fourteen days from the first antigen application.

Another advantage is that, according to the present invention, a far higher yield of antibodies is achieved because, according to the known method, although the antibody titer in the blood serum has increased noticeably, the corresponding age in the milk has already subsided considerably when the first removal is started . But since it aukommt to the optimal antibody titers in the milk rather than those in the blood serum erfindungsgemäöe method not only provides the first antibody in a much shorter time, but "ine overall yield t quite considerably in excess of that of the known methods.

example 1

A strain of Dyßpep.siecoli 0 55 which has been cultivated for several passages in sterile milk is placed on the surface of Z with "Miloeh nutrient agar" precipitated by means of a commercially available tooth

109aU / 20 19. SAp original

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»Eiienüörraig angry. The dishes are incubated over flat (14th stand) in the brood jar "at 39 ° O". The older growth is then washed away with 4-5 ml sterile physiological 0.85% saline solution - (TTaCl) and poured over a glass funnel into egg " Heagetis glass allowed to run in · The germs are killed in a water bath at 62 ° C * N & afa for 20 minutes. Oil transfer into sterile centrifuge glasses is centrifuged at high speed, the supernatant is poured off, replaced by .new sterile HaCl- * LÖBiiög, after whirling up Sediment * centrifuged again _{ο decanted again 3} cleared up with fresh JFaOl solution xmä. Centrifuged uu.s _B f. » about .5-6 eels repeating in the same way * The total bacterial sediment thus washed several times is finally lent "in about 10 ml-sterile physiological UaCl-Iiöauög -aufgeuoimen lai-t" a 20 ml record spray® to a milk oil ί, ττ, ic the liüke Halsve plans .tn; j.lgi®rt _e the antibodies - ".- milk-Gswinnung done already ate aächstfolg © Bd @ m milking and then-ongoing bia SSUM total Ti terabfall.

Example 2

4 p & thogenic Daria germs (Dyspapslecoli 0-55 »0 86 ₉ 0 1-11 and Samonella typhimuriam) are spread (inoculated) in cells on 4 Mhragar plates (Petriso necks) with commercially available hessial leaves. Ansehliessend erfolgt- 18 h _e B © ferüt ~ ung at 37 ° C. Then, the Bsvmchs with physlologtsolier hagl-Lösuug '

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washed away, and transferring a reagent vial by means of a funnel ic. After killing the germs by placing in a 60 ° C temperature controlled Water bath for 30 min (sterile control) and decanting into centrifuge tubes we centrifuged hociitourig, after Übaretand defcantation several times with intermediate centrifugation in a sterile phys. NaCl solution washed. After final sterile The total sediment, washed once again (density Suspension of washed bacteria) in a volume of 10-15 ml Bterilör phys. NaCl solution and taken in a 20 ml whole glass pritae injected into the left jugular vein of a sheep; ready from the next following milking (with a vaccination-related short-term Milk retention is to be expected) the antibody-containing milk, won continuously until the total titer decrease.

Example 3

According to the process technique described in Examples 1 or 2 appropriate amounts of recovered bacterial sedintents (also other pathogenic intestinal bacteria ti) a goat, a cow or another lactating egg intravenously, intraperitoneally or otherwise administered parenterally or orally; each at Next milking will involve the production of antibody milk

began.

1098U / 2019

The antigenic body obtained from verfeforensgeEiäß is 'not harmful to health', as it is obtained by injection of killed antigens (i.e. in the form of a sterile product) in a manner analogous to the usual vaccine production or the older autovaccine production in medical practices

Above and beyond this, the absolute "harmless" it was expanded into Self-curdling in a man proved by daily surrender this antibody milk for months with the best health Successes ,

10981472019

Claims (1)

just about before 1 «Procedure for obtaining milk containing iron specific antibodies and preparations made therefrom, in which antigens are supplied to a dairy animal, a waiting period is inserted and milk enriched with antibodies is removed from the animal, characterized in that with the milk removal for the first time in a period is started in the order of magnitude of twelve or twenty-four hours after the first antigen application, and that further withdrawals follow until the filter drops. 2 · The method according to claim 1, characterized in that the withdrawn milk is broken down into a lipoprotein phase and a skimmed milk phase and that the further extraction of Antibody-containing substance from the first phase follows. 3 «Method according to claim 1 or 2, characterized in that the substance containing the antibodies is conventional food is added "