DE108516C - - Google Patents
Info
- Publication number
- DE108516C DE108516C DENDAT108516D DE108516DA DE108516C DE 108516 C DE108516 C DE 108516C DE NDAT108516 D DENDAT108516 D DE NDAT108516D DE 108516D A DE108516D A DE 108516DA DE 108516 C DE108516 C DE 108516C
- Authority
- DE
- Germany
- Prior art keywords
- acid
- bacilli
- acetic acid
- tuberculinic
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 42
- 239000002253 acid Substances 0.000 claims description 26
- 244000052616 bacterial pathogens Species 0.000 claims description 16
- 239000000126 substance Substances 0.000 claims description 12
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 11
- RTZKZFJDLAIYFH-UHFFFAOYSA-N diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 7
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 7
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 5
- 239000011707 mineral Substances 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 238000003916 acid precipitation Methods 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 230000003053 immunization Effects 0.000 claims description 4
- 150000007513 acids Chemical class 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 102100001249 ALB Human genes 0.000 claims description 2
- 101710027066 ALB Proteins 0.000 claims description 2
- OXNIZHLAWKMVMX-UHFFFAOYSA-N Picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims description 2
- 229940050528 albumin Drugs 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 230000001264 neutralization Effects 0.000 claims description 2
- 229940114148 picric acid Drugs 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000002588 toxic Effects 0.000 claims description 2
- 231100000331 toxic Toxicity 0.000 claims description 2
- 229950002929 trinitrophenol Drugs 0.000 claims description 2
- 239000002244 precipitate Substances 0.000 claims 9
- 238000010790 dilution Methods 0.000 claims 3
- 239000012458 free base Substances 0.000 claims 2
- TZCXTZWJZNENPQ-UHFFFAOYSA-L Barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 claims 1
- 238000009631 Broth culture Methods 0.000 claims 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 1
- 229910052601 baryte Inorganic materials 0.000 claims 1
- 239000010428 baryte Substances 0.000 claims 1
- 230000000875 corresponding Effects 0.000 claims 1
- 239000012531 culture fluid Substances 0.000 claims 1
- 239000000839 emulsion Substances 0.000 claims 1
- 238000001704 evaporation Methods 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 230000002085 persistent Effects 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- ZVUUCUFDAHKLKT-UHFFFAOYSA-M sodium;2,4,6-trinitrophenolate Chemical compound [Na+].[O-]C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O ZVUUCUFDAHKLKT-UHFFFAOYSA-M 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 230000002378 acidificating Effects 0.000 description 3
- 231100000614 Poison Toxicity 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- -1 sulfuric acid Chemical class 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/04—Mycobacterium, e.g. Mycobacterium tuberculosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Pulmonology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Saccharide Compounds (AREA)
- Compounds Of Unknown Constitution (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
KAISERLICHESIMPERIAL
PATENTAMT.PATENT OFFICE.
PATENTSCHRIFTPATENT LETTERING
KLASSE 30: Gesundheitspflege.CLASS 30: Health Care.
bzw. Tuberkelbacillencülturen.or tubercle bacilli cultures.
Patentirt im Deutschen Reiche vom 29. September 1898 ab.Patented in the German Empire on September 29, 1898.
Ueber den Chemismus der Tuberkelbacillen angestellte Untersuchungen haben gezeigt, dafs diese Bacillen eine säureartige Substanz sowohl in freiem, wie auch in gebundenem Zustande enthalten und dafs den salzartigen Verbindungen dieser Säure, weit weniger der sä'ureartigen Substanz selbst, einerseits hervorragend- giftige, andererseits aber auch immunisirende Eigenschaften zukommen.Investigations made on the chemistry of the tubercle bacilli have shown that these bacilli have an acidic substance both in the free and in the bound state and that the salt-like compounds of this acid, far less of the acidic ones Substance itself, on the one hand extremely toxic, but on the other hand also immunizing properties get.
Die säureartige Substanz, welche die allgemeinen Eigenschaften einer Nucle'insäure besitzt, soll Tuberculinsäure, ihre salzartigen Verbindungen dagegen sollen tuberculinsaures Tuberculosamin bezw. tuberculinsaures Albumin genannt werden.The acidic substance, which has the general properties of a nucleic acid, is said to be tuberculinic acid, while its salt-like compounds are said to be tuberculinic acid tuberculosamine respectively tuberculinic acid albumin.
Zur Darstellung dieser Substanzen wird folgendermafsen verfahren:The following is used to illustrate these substances procedure:
Die Bacillen werden durch Filtriren von der Culturflüssigkeit getrennt, mit ■ sterilisirtem Wasser sorgfältig, und zwar so lange gewaschen, bis alle Schleim- oder mucinartigen Körper entfernt sind. Die gewaschenen Bacillen werden dann im Exsiccator über Schwefelsäure scharf getrocknet, gut zerkleinert und mit grofsen Mengen Wasser ausgelaugt. Nach der Extraction werden die Bacillen durch Centnfugiren von der Flüssigkeit getrennt. Aus der völlig klaren Flüssigkeit schlagen Essigsäure und Mineralsäuren den als tuberculinsaures Tuberculosainin bezeichneten Körper nieder, während die als Tuberculinsäure bezeichnete freie Säure durch Essigsäure nicht fällbar ist.The bacilli are separated from the culture liquid by filtration, with sterilized liquid Water carefully, washing until all mucous or mucinous Bodies are removed. The washed bacilli are then in a desiccator over sulfuric acid sharply dried, well chopped and leached with large quantities of water. After Extraction, the bacilli are separated from the liquid by centnfugiren. From the completely clear liquid, acetic acid and mineral acids beat that as tuberculinic acid Tuberculosainin designated body, while that designated as tuberculinic acid free acid cannot be precipitated by acetic acid.
Diese Säure wird aus den Filtraten der Essigsäurefällung durch salzsäurehaltigen Alkohol niedergeschlagen.This acid is obtained from the filtrates of acetic acid precipitation with alcohol containing hydrochloric acid dejected.
Da die Tuberculinsäure in der durch Essigsäure niedergeschlagenen Substanz in gebundener Form enthalten ist, da ferner der Giftwerth der Essigsäurefällung den Wirkungswerth der freien Säure bei Weitem übertrifft, so konnte von vornherein angenommen werden, dafs man zu hochwerthigen Präparaten gelangt, wenn man aus der salzartigen, mittels Essigsäure gefällten Verbindung durch Entfernung der Säure den basischen Paarling isolirt.Since the tuberculinic acid is bound in the substance precipitated by acetic acid Form is included, since the poisonous value of acetic acid precipitation also has the effect value by far exceeds the free acid, it could be assumed from the outset that that one arrives at high-quality preparations if one leaves the salt-like, means Acetic acid precipitated compound by removing the acid the basic pair isolated.
Zu diesem Ergebnifs kann man auf verschiedenen Wegen gelangen: einmal, indem man den neutralen Körper mit verdünnten Mineralsäuren, z.B. Schwefelsäure, innig mischt und längere Zeit sehr gründlich schüttelt, wobei der basische· Körper als Salz der betreffenden Mineralsäure, ζ. B. als Sulfat, in Lösung geht, oder aber dadurch, dafs man durch vorsichtiges Erwärmen mit verdünnten Laugen eine Spaltung herbeiführt.This result can be reached in different ways: once, by the neutral body intimately mixes with dilute mineral acids, e.g. sulfuric acid, and Shakes very thoroughly for a long time, the alkaline body being the salt of the concerned Mineral acid, ζ. B. as sulfate, goes into solution, or by the fact that one by careful Warming with dilute alkalis causes cleavage.
Aus den Lösungen wird im ersten Falle das Tuberculosaminsulfat als völlig farbloses, lockeres Pulver niedergeschlagen. Zur Reinigung führt man das Sulfat am besten in das entsprechende Pikrat über, aus welchem man durch Behandeln mit Schwefelsäure das Sulfat wieder herstellen und durch Ausschütteln mit Aether die Pikrinsäure entfernen kann.In the first case, the solutions turn into tuberculosamine sulphate as a completely colorless, loose substance Powder down. The best way to purify the sulfate is to add it to the appropriate one Picrat over, from which one can restore the sulphate by treatment with sulfuric acid and can remove the picric acid by shaking with ether.
Claims (3)
Publications (1)
Publication Number | Publication Date |
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DE108516C true DE108516C (en) |
Family
ID=378488
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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DENDAT108516D Active DE108516C (en) |
Country Status (1)
Country | Link |
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DE (1) | DE108516C (en) |
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- DE DENDAT108516D patent/DE108516C/de active Active
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