DE102007021968A1 - Microscopic object slide for use in microscopic investigations, has flat bars proceeding into suction tube with tapered tube-mantel and into tube-mantled end piece, where set of durable seals is connected with slide and cover glass - Google Patents

Microscopic object slide for use in microscopic investigations, has flat bars proceeding into suction tube with tapered tube-mantel and into tube-mantled end piece, where set of durable seals is connected with slide and cover glass Download PDF

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Publication number
DE102007021968A1
DE102007021968A1 DE200710021968 DE102007021968A DE102007021968A1 DE 102007021968 A1 DE102007021968 A1 DE 102007021968A1 DE 200710021968 DE200710021968 DE 200710021968 DE 102007021968 A DE102007021968 A DE 102007021968A DE 102007021968 A1 DE102007021968 A1 DE 102007021968A1
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Prior art keywords
tube
slide
cover glass
end piece
microscopic
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DE200710021968
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German (de)
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Roland Birke
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/11—Filling or emptying of cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/058—Flat flow cell

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The slide (1) has an observation field (3) formed by a cover glass (2) with a durable, sealed or glued round or flat bars (4). The flat bars proceed into a suction tube with a tapered tube-mantel (5) and into a tube-mantled end piece (6). A set of durable seals (7) is connected with the slide and the cover glass. The observation field remains open to inside of the slide. The suction tube with the tapered tube mantel and the tube-mantled end piece are tapered to approximately 20 degrees. A deflector is provided at the tube-mantled end piece.

Description

Es sind mikroskopische Objektträger bekannt, die nach dem physikalischen Prinzip kommunizierender Röhren funktionieren. Hierbei werden Flüssigkeiten mit den zu untersuchenden Partikeln in Gefäße gefüllt, um sie bei geeigneter Tiefenschärfe im Beobachtungsfeld mikroskopieren zu können. ( DE 43 34 677 C1 )Microscopic slides are known which function according to the physical principle of communicating tubes. In this case, liquids are filled with the particles to be examined in vessels in order to be able to microscopy them with a suitable depth of focus in the field of observation. ( DE 43 34 677 C1 )

Der im Patentanspruch 1 angegebenen Erfindung liegt das Problem zugrunde, daß die zu untersuchenden Medien mit separaten Pipetten in die Gefäße gefüllt werden müssen.Of the The invention defined in claim 1 is based on the problem that the media to be examined with separate pipettes must be filled in the vessels.

Dieses Problem wird durch einen Objektträger, auf dem sich eine zweigeteilte Pipette mit einem Beobachtungsfeld befindet, gemäß 1 gelöst. Die Zeichnung 1 zeigt die Erfindung von schräg oben. Die Zeichnung 2 zeigt einen Teilquerschnitt.This problem is determined by a slide on which there is a two-part pipette with an observation field 1 solved. The drawing 1 shows the invention from above obliquely. The drawing 2 shows a partial cross section.

Die Herstellung erfolgt durch dichte Verbindung (7) der Stirnseite eines verjüngten Rohrmantels (5) mit einem mikroskopischen Objektträger (1) und einem Deckglas (2) wobei das offene Kanalende des Beobachtungsfeldes (3) in den Rohrmantel (5) mündet. Das Beobachtungsfeld (3) entsteht durch dauerhaft dichtender Montage eines Deckglases (2) mit zweier parallel angeordneter Rund- oder Flachstäbe (4) auf einem Objektträger (1). Das zweite Kanalende des Beobachtungsfeldes (3) mündet in ein Rohrmantelendstück (6), dessen Stirnseite ebenfalls mit dem Objektträger und dem Deckglas dicht verbunden ist. Ein Sauger (8) am Rohrmantelendstück komplettiert die Erfindung. Die mit der Erfindung erzielten Vorteile bestehen darin, daß die mit der Pipette aufgenommenen Medien sofort im Beobachtungsfeld (3) mikroskopisch untersucht werden können. Eine vorteilhafte Ausgestaltung der Erfindung ist im Patentanspruch 2 angegeben (2), wobei die im ca. 20 Grad-Winkel abgeschrägten Stirnseiten der Rohrmantelstücke im 90 Grad-Winkel montiert sind und somit eine gute Handhabung der Erfindung ermöglicht.The production takes place by tight connection ( 7 ) of the end face of a tapered tubular jacket ( 5 ) with a microscope slide ( 1 ) and a coverslip ( 2 ) wherein the open channel end of the observation field ( 3 ) in the pipe jacket ( 5 ) opens. The observation field ( 3 ) is formed by permanently sealing mounting of a cover glass ( 2 ) with two parallel arranged round or flat bars ( 4 ) on a microscope slide ( 1 ). The second channel end of the observation field ( 3 ) flows into a tube jacket end piece ( 6 ), whose front side is also tightly connected to the slide and the cover glass. A sucker ( 8th ) on Rohrmantelendstück completes the invention. The advantages achieved by the invention are that the recorded with the pipette media immediately in the field of observation ( 3 ) can be examined microscopically. An advantageous embodiment of the invention is specified in claim 2 ( 2 ), wherein the beveled in about 20 degrees angle end faces of the pipe jacket pieces are mounted at 90 degree angle, thus enabling a good handling of the invention.

Die Erfindung gestattet die Aufnahme flüssiger Medien mit kleinen Partikeln, die sofort im Lichtstrahlengang handelsüblicher Mikroskope untersucht werden können. Das Deckglas (2) mit 0,15 mm Dicke, befindet sich in korrekter Planlage und ermöglicht mikroskopische Kontrastierverfahren wie z. B. Hellfeld, Dunkelfeld, Phasenkontrast oder differenzieller Interferenzkontrast. Nach Gebrauch kann die Erfindung durch einfaches Durchspülen gereinigt und wiederverwendet werden – hierbei wird die Pipettenspitze in Reinigungslösung getaucht, während man mit dem Sauger (8) pumpt. Auf einfache und schnelle Weise können auf diese Art mikroskopische Proben in Serie hergestellt und untersucht werden. Möglich ist auch die Kultur oder das Zusammenführen verschiedener Mikroorganismen im Millilitermaßstab.The invention allows the recording of liquid media with small particles that can be examined immediately in the light beam of commercially available microscopes. The cover glass ( 2 ) with 0.15 mm thickness, is in the correct flatness and allows microscopic contrasting methods such. As bright field, dark field, phase contrast or differential interference contrast. After use, the invention can be cleaned and reused simply by rinsing - in this case, the pipette tip is immersed in cleaning solution, while with the sucker ( 8th ) pumps. In a simple and fast way, microscopic samples can be mass-produced and analyzed in this way. It is also possible the culture or the merging of various microorganisms on the milliliter scale.

Anwendungen können hydrobiologische Laboruntersuchungen, limnologische und marine Planktonuntersuchungen oder die mikroskopische Kontrolle der Belebtschlammflocken von Kläranlagen sein.applications can hydrobiological laboratory studies, limnological and marine plankton examinations or microscopic control be the activated sludge flakes of sewage treatment plants.

Die Erfindung kann auch dazu verwendet werden, flüssige Medien (z. B. Vitalfarbstoffe, Nährlösungen, Narkotika oder Chemikalien) zur Probe hinzuzufügen und durch leichtes Pumpen zu Durchmischen – wobei Reaktionen mikroskopisch untersucht werden können. Die Pipettenspitze vorübergehend zu Verschließen (z. B. mit Silikon) und somit ein vor Austrocknung geschütztes Dauerpräparat zu fertigen ist eine weitere Anwendungsmöglichkeit – hierbei können z. B. thermische oder radiologische Manipulationen durchgeführt werden.The Invention can also be used to liquid media (eg vital dyes, nutrient solutions, narcotics or chemicals) to the sample and by light Pumps to mix - taking reactions microscopically can be examined. Temporarily close the pipette tip Close (eg with silicone) and thus one before dehydration To manufacture protected permanent preparation is one further application - this can z. B. thermal or radiological manipulations performed become.

Die Teile des Pipettierobjektträgers bestehen aus Glas oder Kunststoff.The Parts of the pipetting slide are made of glass or Plastic.

Die dichtenden und dauerhaften Verbindungen sollten durch schmelzende Verbindungen hergestellt werden oder es sollten Kleber verwendet werden, die eine hohe Endfestigkeit mit glasähnlichen Eigenschaften (Beständigkeit gegenüber Temperatur, Wasser, Lösungsmittel, verdünnte Säuren und Laugen sowie Chemikalien) erreichen, z. B. UV-Kleber.The Sealing and permanent connections should be through melting Connections or adhesives should be used which have a high final strength with glass-like properties (Resistance to temperature, water, solvents, diluted acids and bases and chemicals) reach, z. B. UV adhesive.

Die Wärmebeständigkeit ermöglicht fraktionierte Dampfsterilisation – hierbei empfiehlt sich die Verwendung eines Pipettensaugers aus Silikon.The Heat resistance allows fractionated Steam sterilization - this is recommended for use a silicone vacuum cleaner.

11
Mikroskopischer Objektträger (ca. 76 mm × 26 mm).microscopic Slide (about 76 mm × 26 mm).
22
Deckglas (ca. 0,15 mm stark).cover glass (about 0.15 mm thick).
33
Beobachtungsfeld (ca. 45 mm × 8 mm).observation field (about 45 mm × 8 mm).
44
Dauerhaft dichtend verklebte Rund- oder Flachstäbe.Permanent sealing glued round or flat bars.
55
Saugrohr mit einem verjüngten Rohrmantel.suction tube with a tapered tube jacket.
66
Rohrmantelendstück.Rohrmantelendstück.
77
Dauerhaft dichtende Verklebung.Permanent sealing bonding.
88th
Sauger.Sucker.

ZITATE ENTHALTEN IN DER BESCHREIBUNGQUOTES INCLUDE IN THE DESCRIPTION

Diese Liste der vom Anmelder aufgeführten Dokumente wurde automatisiert erzeugt und ist ausschließlich zur besseren Information des Lesers aufgenommen. Die Liste ist nicht Bestandteil der deutschen Patent- bzw. Gebrauchsmusteranmeldung. Das DPMA übernimmt keinerlei Haftung für etwaige Fehler oder Auslassungen.This list The documents listed by the applicant have been automated generated and is solely for better information recorded by the reader. The list is not part of the German Patent or utility model application. The DPMA takes over no liability for any errors or omissions.

Zitierte PatentliteraturCited patent literature

  • - DE 4334677 C1 [0001] - DE 4334677 C1 [0001]

Claims (3)

Objektträger mit Beobachtungsfeld für mikroskopische Untersuchungen, dadurch gekennzeichnet, daß ein Beobachtungsfeld, welches durch ein Deckglas (2) mit dauerhaft dichtend verklebter Rund- oder Flachstäbe (4) gebildet wird, in ein Saugrohr mit einem verjüngten Rohrmantel (5) und in ein Rohrmantelendstück (6) mündet und mit einem Objektträger (1) und dem Deckglas (2) dauerhaft dichtend (7) verbunden sind, wobei das Beobachtungsfeld innen offen bleibt (3) gemäß 1, wobei 1 Bestandteil des Anspruchs ist.Microscope slide with observation field for microscopic examinations, characterized in that an observation field which passes through a cover glass ( 2 ) with permanently sealed glued round or flat bars ( 4 ) is formed in a suction pipe with a tapered pipe jacket ( 5 ) and in a Rohrmantelendstück ( 6 ) and with a slide ( 1 ) and the cover glass ( 2 ) permanently sealed ( 7 ), leaving the field of observation inside ( 3 ) according to 1 , in which 1 Part of the claim is. Objektträger mit Beobachtungsfeld nach Patentanspruch 1, dadurch gekennzeichnet, daß das Saugrohr mit verjüngtem Rohrmantel (5) und das Rohrmantelendstück (6) ca. 20 Grad abgeschrägt sind und im 90 Grad-Winkel mit dem Objektträger (1) und dem Deckglas (2) verbunden sind (2), wobei 2 Bestandteil des Anspruchs ist.Slide with observation field according to claim 1, characterized in that the suction tube with a tapered tube jacket ( 5 ) and the tube shell end piece ( 6 ) are approximately 20 degrees beveled and at 90 degrees angle with the slide ( 1 ) and the cover glass ( 2 ) are connected ( 2 ), in which 2 Part of the claim is. Objektträger mit Beobachtungsfeld nach Patentanspruch 2, dadurch gekennzeichnet, daß sich am Rohrmantelendstück (6) ein Sauger (8) befindet.Microscope with observation field according to claim 2, characterized in that at the tubular jacket end piece ( 6 ) a sucker ( 8th ) is located.
DE200710021968 2007-05-10 2007-05-10 Microscopic object slide for use in microscopic investigations, has flat bars proceeding into suction tube with tapered tube-mantel and into tube-mantled end piece, where set of durable seals is connected with slide and cover glass Withdrawn DE102007021968A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE200710021968 DE102007021968A1 (en) 2007-05-10 2007-05-10 Microscopic object slide for use in microscopic investigations, has flat bars proceeding into suction tube with tapered tube-mantel and into tube-mantled end piece, where set of durable seals is connected with slide and cover glass

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE200710021968 DE102007021968A1 (en) 2007-05-10 2007-05-10 Microscopic object slide for use in microscopic investigations, has flat bars proceeding into suction tube with tapered tube-mantel and into tube-mantled end piece, where set of durable seals is connected with slide and cover glass

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DE102007021968A1 true DE102007021968A1 (en) 2008-11-20

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DE200710021968 Withdrawn DE102007021968A1 (en) 2007-05-10 2007-05-10 Microscopic object slide for use in microscopic investigations, has flat bars proceeding into suction tube with tapered tube-mantel and into tube-mantled end piece, where set of durable seals is connected with slide and cover glass

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102401827A (en) * 2010-09-10 2012-04-04 孔维顺 Detection slide
CN104730701A (en) * 2015-04-02 2015-06-24 刘洪瑞 Self-cleaning type microscope specimen storage device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4334677C1 (en) 1993-03-26 1994-07-28 Roland Birke Culture vessel

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4334677C1 (en) 1993-03-26 1994-07-28 Roland Birke Culture vessel

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102401827A (en) * 2010-09-10 2012-04-04 孔维顺 Detection slide
CN104730701A (en) * 2015-04-02 2015-06-24 刘洪瑞 Self-cleaning type microscope specimen storage device
CN104730701B (en) * 2015-04-02 2017-07-04 刘洪瑞 A kind of self-cleaning microscope example apparatus for placing

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