DE102006053474A1 - Flow chamber for use in substrate-integrated microelectrode array, has connections for fluid inlet and outlet, where chamber is glued on flat surface for production of laminar flow inside chamber and inner side coated with inert plastic - Google Patents

Flow chamber for use in substrate-integrated microelectrode array, has connections for fluid inlet and outlet, where chamber is glued on flat surface for production of laminar flow inside chamber and inner side coated with inert plastic Download PDF

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Publication number
DE102006053474A1
DE102006053474A1 DE200610053474 DE102006053474A DE102006053474A1 DE 102006053474 A1 DE102006053474 A1 DE 102006053474A1 DE 200610053474 DE200610053474 DE 200610053474 DE 102006053474 A DE102006053474 A DE 102006053474A DE 102006053474 A1 DE102006053474 A1 DE 102006053474A1
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Germany
Prior art keywords
chamber
outlet
laminar flow
glued
connections
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DE200610053474
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German (de)
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DR HORST LOHMANN DIACLEAN GmbH
Horst Lohmann Diaclean Dr GmbH
Original Assignee
DR HORST LOHMANN DIACLEAN GmbH
Horst Lohmann Diaclean Dr GmbH
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Priority to DE200610053474 priority Critical patent/DE102006053474A1/en
Publication of DE102006053474A1 publication Critical patent/DE102006053474A1/en
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)

Abstract

The chamber (1) has connections (2) for fluid inlet and outlet, where the chamber is glued on a flat surface for the production of a laminar flow inside the chamber. An inner side of the chamber is designed such that a laminar flow over an opening (3) is adjusted, where the construction of the inlet and the outlet is such that a constant fluid level of three millimeters is reached over a chamber base. The inner side of the chamber is coated with an inert plastic such as polytetrafluoroethylene (PTFE).

Description

Die Erfindung betrifft eine Vorrichtung entsprechend dem Oberbegriff des Anspruchs I.The The invention relates to a device according to the preamble of claim I.

Um mit substratintegrierten Mikroelektrodenarrays reproduzierbare und aussagekräftige potentiometrische oder amperometrische Messungen an in vitro Präparationen wie Gewebeschnitten oder Zellkulturen durchführen zu können, ist eine möglichst laminare Superfusion des Gewebes mit sauerstoffangereicherter Nährstofflösung notwendig.Around reproducible with substrate-integrated microelectrode arrays and meaningful potentiometric or amperometric measurements on in vitro preparations How to perform tissue sections or cell cultures is one possible laminar superfusion of the tissue with oxygenated nutrient solution necessary.

Bisher werden Mikroelektrodenarrays mit einem aufgeklebten Plastikring eingesetzt, die durch Verwendung von Stahl- oder Kunststoffkanülen mit einem Lösungszulauf und -ablauf ausgestattet werden können. Diese Systeme besitzen den wesentlichen Nachteil, dass die Plastikringe nicht erneut auf einem anderen Elektrodenarray eingesetzt werden können, und dass an der Oberfläche der Flüssigkeit in der Kammer durch die Absaugung der Flüssigkeit Vibrationen auftreten und der Flüssigkeitsspiegel nur unter Zuhilfenahme komplexer optisch geregelter Systeme über lange Zeit stabil erhalten werden kann.So far are microelectrode arrays with a glued plastic ring used by using steel or plastic cannulas with a solution inlet and -ablauf can be equipped. Own these systems the main drawback that the plastic rings are not on one again other electrode array can be used, and that on the surface of Liquid in the chamber through the suction of the liquid vibrations occur and the liquid level only with the help of complex optically controlled systems over a long time Time can be stably maintained.

Eine laminare Strömung in Verbindung mit einer Homogenität der Oberfläche ist daher nicht mehr gegeben, was zu enormen Schwankungen der Signalamplituden und erhöhtem Signalrauschen führt.A laminar flow in connection with a homogeneity of the surface therefore no longer given, causing huge fluctuations in signal amplitudes and elevated Signal noise leads.

Aufgabe der Erfindung ist es, einen Flüssigkeitsstrom innerhalb einer strömungsgünstig konstruierten und aufgeklebten Kammer zu erzeugen, der diesen Effekt verhindert. Außerdem soll die Kammer auf anderen Mikroelektrodenarrays wieder verwendbar sein.task The invention is a liquid flow within a streamlined design and glued chamber to prevent this effect. Furthermore the chamber should be reusable on other microelectrode arrays be.

Diese Aufgaben werden durch eine Vorrichtung mit den Merkmalen des Anspruches I gelöst.These Tasks are achieved by a device having the features of the claim I solved.

Durch einen integrierten Zu- und Ablauf, sowie den strömungsgünstigen Aufbau der Kammer wird ein kontinuierlicher Flüssigkeitsstrom erzeugt.By an integrated inlet and outlet, and the streamlined construction of the chamber is a continuous liquid flow generated.

Anders als bei den bisherigen Systemen findet so keine Beeinflussung durch zusätzliche Zu- und Ableitungen innerhalb der Flüssigkeit statt.Different as in the previous systems is so by no influence additional Inlets and outlets inside the liquid instead of.

Durch das Aufkleben der Durchflusskammer mit einem Silikonkleber wird sowohl ein Ablösen, als auch eine optimale Abdichtung der Kammer, und somit letzendlich ein flexibler Einsatz ermöglicht.By Sticking the flow chamber with a silicone adhesive is both a detachment, as well as an optimal seal of the chamber, and thus ultimately allows a flexible use.

Ausführungsbeispiele der Erfindung sind in den Zeichnungen dargestellt und werden im Folgenden näher beschrieben.embodiments The invention are illustrated in the drawings and are in Following closer described.

Es zeigenIt demonstrate

I: eine perspektivische Ansicht der Kammer I : a perspective view of the chamber

II: eine Aufsicht der Kammer II : a supervision of the chamber

III: einen senkrechten Schnitt durch die Kammer. III : a vertical section through the chamber.

Für die Fertigung der Kammer gibt es prinzipiell zwei Möglichkeiten.For the production There are basically two options for the chamber.

Zum Einen kann ein Spritzgussverfahren angewendet werden, und zum Anderen kann zur Formgebung ein spanendes Verfahren eingesetzt werden (z.B. CNC-Fräse).To the One can be applied an injection molding process, and on the other For example, a machining method (e.g. CNC milling machine).

Beim Spritzgussverfahren wird mit einer Spritzgießmaschine die Formmasse in einer Spritzeinheit plastifiziert und in ein Spritzgießwerkzeug eingespritzt. Der Hohlraum, die Kavität, des Werkzeugs bestimmt die Form und die Oberflächenstruktur des fertigen Teils. Als Formmasse sind transparente Kunststoffe wie Acrylglas denkbar, weil sie eine gute Beleuchtung der biologischen Präparate erlauben. Zur Vermeidung von Adhäsionsphänomenen kann die innere Oberfläche der Kammern mit inerten Kunststoffen veredelt werden (z.B. durch PTFE).At the Injection molding is using an injection molding machine, the molding compound in an injection unit plasticized and injected into an injection mold. The cavity, the cavity, of the tool determines the shape and surface texture of the finished part. As a molding compound transparent plastics such as acrylic glass are conceivable, because they allow a good lighting of the biological preparations. To avoid adhesion phenomena the inner surface the chambers are refined with inert plastics (e.g. PTFE).

Für große Stückzahlen ist dieses Herstellungsprinzip von Vorteil.For large quantities this manufacturing principle is an advantage.

Die CNC-Technik als spanendes Verfahren ermöglicht ebenfalls die Erzeugung von dreidimensionalen Strukturen. Anders als das Spritzgussverfahren eignet sich dieses Prinzip besonders bei kleineren Stückzahlen oder wenn anwenderspezifische Modifikationen und Erweiterungen gewünscht werden.The CNC technology as a cutting process also enables production of three-dimensional structures. Unlike the injection molding process This principle is particularly suitable for smaller quantities or if user-specific modifications and extensions are desired.

Die Kammer (1) besitzt zwei Bohrungen (2), durch die Flüssigkeiten mit Hilfe einer externen Pumpe durch die Kammer gepumpt werden können.The chamber ( 1 ) has two holes ( 2 ), through which fluids can be pumped through the chamber by means of an external pump.

Durch die strömungsgünstige Formgebung entsteht so eine laminare Strömung über der Öffnung (3) auf der Unterseite der Kammer.Due to the streamlined shape, a laminar flow is created over the opening ( 3 ) on the bottom of the chamber.

Claims (6)

Auf eine plane Oberfläche aufklebbare Durchflusskammer zur Erzeugung eines laminaren Flusses im Inneren der Kammer (1), dadurch gekennzeichnet, dass die Kammer (1) bereits mit Anschlüssen (2) für einen Zu- und Ablauf versehen ist.Flowable chamber adhered to a flat surface for generating a laminar flow inside the chamber ( 1 ), characterized in that the chamber ( 1 ) already with connections ( 2 ) is provided for an inlet and outlet. Vorrichtung nach Anspruch I, dadurch gekennzeichnet, dass das Innere der Kammer (1) so konstruiert ist, dass sich ein laminarer Fluss über der Öffnung (3) einstellt.Device according to claim 1, characterized in that the interior of the chamber ( 1 ) is constructed so that a laminar flow over the opening ( 3 ). Vorrichtung nach Anspruch I oder 2, dadurch gekennzeichnet, dass durch die Konstruktion des Flüssigkeitszulaufs- und ablaufs ein konstanter Flüssigkeitsspiegel in 3 mm Höhe über dem Kammerboden erreicht wird.Device according to claim 1 or 2, characterized that by the construction of the liquid inlet and outlet a constant level of fluid in 3 mm height above the Chamber bottom is reached. Vorrichtung nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass das Ein- und Abpumpen von Flüssigkeit die Homogenität der Oberfläche dieser Flüssigkeit nicht beeinflusst.Device according to one of the preceding claims, characterized characterized in that the pumping in and out of liquid the homogeneity the surface this liquid unaffected. Vorrichtung nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass die Kammer aufklebbar, ablösbar und wieder verwendbar ist auf kommerziellen Mikroelektrodenarrays.Device according to one of the preceding claims, characterized in that the chamber can be glued on, removed and reused is on commercial microelectrode arrays. Vorrichtung nach einem der vorherigen Ansprüche, dadurch gekennzeichnet, dass die Möglichkeit besteht, das Innere der Kammer mit einem inerten Kunststoff wie z.B. PTFE zu beschichten.Device according to one of the preceding claims, characterized that marked the possibility consists of the interior of the chamber with an inert plastic like e.g. PTFE to coat.
DE200610053474 2006-11-14 2006-11-14 Flow chamber for use in substrate-integrated microelectrode array, has connections for fluid inlet and outlet, where chamber is glued on flat surface for production of laminar flow inside chamber and inner side coated with inert plastic Withdrawn DE102006053474A1 (en)

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DE200610053474 DE102006053474A1 (en) 2006-11-14 2006-11-14 Flow chamber for use in substrate-integrated microelectrode array, has connections for fluid inlet and outlet, where chamber is glued on flat surface for production of laminar flow inside chamber and inner side coated with inert plastic

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010089226A1 (en) * 2009-02-04 2010-08-12 Siemens Aktiengesellschaft Arrangement and method for electrochemically measuring biochemical reactions and method for producing the arrangement

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4742708A (en) * 1986-08-13 1988-05-10 Beckman Industrial Corporation Apparatus for electrochemical sensor calibration
EP0811841A1 (en) * 1996-06-06 1997-12-10 ENDRESS + HAUSER CONDUCTA GESELLSCHAFT FÜR MESS UND REGELTECHNIK mbH & Co. Electrochemical gas sensor arrangement
EP1031833A2 (en) * 1999-02-22 2000-08-30 Ngk Spark Plug Co., Ltd Gas sensor device
WO2002094441A2 (en) * 2001-05-23 2002-11-28 Nanostream, Inc. Fluidic systems with non-planar microfluidic devices
US6494230B2 (en) * 1998-01-20 2002-12-17 Caliper Technologies Corp. Multi-layer microfluidic devices
DE102004033440A1 (en) * 2004-07-08 2006-02-02 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Microstructured device and method for its production
DE102004056516A1 (en) * 2004-11-24 2006-06-01 Drägerwerk AG Method for attaching flexible, electrochemical sensors

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4742708A (en) * 1986-08-13 1988-05-10 Beckman Industrial Corporation Apparatus for electrochemical sensor calibration
EP0811841A1 (en) * 1996-06-06 1997-12-10 ENDRESS + HAUSER CONDUCTA GESELLSCHAFT FÜR MESS UND REGELTECHNIK mbH & Co. Electrochemical gas sensor arrangement
US6494230B2 (en) * 1998-01-20 2002-12-17 Caliper Technologies Corp. Multi-layer microfluidic devices
EP1031833A2 (en) * 1999-02-22 2000-08-30 Ngk Spark Plug Co., Ltd Gas sensor device
WO2002094441A2 (en) * 2001-05-23 2002-11-28 Nanostream, Inc. Fluidic systems with non-planar microfluidic devices
DE102004033440A1 (en) * 2004-07-08 2006-02-02 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Microstructured device and method for its production
DE102004056516A1 (en) * 2004-11-24 2006-06-01 Drägerwerk AG Method for attaching flexible, electrochemical sensors

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010089226A1 (en) * 2009-02-04 2010-08-12 Siemens Aktiengesellschaft Arrangement and method for electrochemically measuring biochemical reactions and method for producing the arrangement
CN102369432A (en) * 2009-02-04 2012-03-07 西门子公司 Arrangement and method for electrochemically measuring biochemical reactions and method for producing the arrangement
US8920727B2 (en) 2009-02-04 2014-12-30 Boehringer Ingelheim Vetmedica Gmbh Arrangement and method for electrochemically measuring biochemical reactions and method for producing the arrangement

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Effective date: 20131115