DE10131899A1 - In vitro-Screening-Assay für gamma-Secretase - Google Patents

In vitro-Screening-Assay für gamma-Secretase

Info

Publication number
DE10131899A1
DE10131899A1 DE10131899A DE10131899A DE10131899A1 DE 10131899 A1 DE10131899 A1 DE 10131899A1 DE 10131899 A DE10131899 A DE 10131899A DE 10131899 A DE10131899 A DE 10131899A DE 10131899 A1 DE10131899 A1 DE 10131899A1
Authority
DE
Germany
Prior art keywords
ctfγ
secretase activity
secretase
activity
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE10131899A
Other languages
German (de)
English (en)
Inventor
Klaus Fuchs
Cornelia Dorner-Ciossek
Marcus Kostka
Christian Haass
Harald Steiner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Boehringer Ingelheim Pharma GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma GmbH and Co KG filed Critical Boehringer Ingelheim Pharma GmbH and Co KG
Priority to DE10131899A priority Critical patent/DE10131899A1/de
Priority to US10/183,119 priority patent/US20030022251A1/en
Priority to CA002452832A priority patent/CA2452832A1/fr
Priority to PCT/EP2002/007095 priority patent/WO2003008635A2/fr
Priority to EP02745414A priority patent/EP1407043A2/fr
Priority to JP2003514950A priority patent/JP2004535206A/ja
Priority to MXPA03011898A priority patent/MXPA03011898A/es
Publication of DE10131899A1 publication Critical patent/DE10131899A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychiatry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE10131899A 2001-07-04 2001-07-04 In vitro-Screening-Assay für gamma-Secretase Withdrawn DE10131899A1 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE10131899A DE10131899A1 (de) 2001-07-04 2001-07-04 In vitro-Screening-Assay für gamma-Secretase
US10/183,119 US20030022251A1 (en) 2001-07-04 2002-06-26 Gamma-secretase in vitro screening assay
CA002452832A CA2452832A1 (fr) 2001-07-04 2002-06-27 Essai de criblage in vitro de la .gamma.-secretase
PCT/EP2002/007095 WO2003008635A2 (fr) 2001-07-04 2002-06-27 Essai de criblage in vitro de $g(g)-secretase
EP02745414A EP1407043A2 (fr) 2001-07-04 2002-06-27 Essai de criblage in vitro de gamma-secretase
JP2003514950A JP2004535206A (ja) 2001-07-04 2002-06-27 γ−セクレターゼのｉｎｖｉｔｒｏスクリーニングアッセイ
MXPA03011898A MXPA03011898A (es) 2001-07-04 2002-06-27 Ensayo de seleccion in vitro para ?- secretasa.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10131899A DE10131899A1 (de) 2001-07-04 2001-07-04 In vitro-Screening-Assay für gamma-Secretase

Publications (1)

Publication Number Publication Date
DE10131899A1 true DE10131899A1 (de) 2003-02-27

Family

ID=7690254

Family Applications (1)

Application Number Title Priority Date Filing Date
DE10131899A Withdrawn DE10131899A1 (de) 2001-07-04 2001-07-04 In vitro-Screening-Assay für gamma-Secretase

Country Status (6)

Country Link
EP (1) EP1407043A2 (fr)
JP (1) JP2004535206A (fr)
CA (1) CA2452832A1 (fr)
DE (1) DE10131899A1 (fr)
MX (1) MXPA03011898A (fr)
WO (1) WO2003008635A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0229582D0 (en) * 2002-12-19 2003-01-22 Merck Sharp & Dohme Novel assay
DK1849762T3 (da) 2006-04-21 2009-10-12 Cellzome Ltd Substituerede biphenylcarboxylsyrer og derivater deraf
EA200970838A1 (ru) 2007-03-07 2010-02-26 Янссен Фармацевтика Н.В. ЗАМЕЩЕННЫЕ ФЕНОКСИПРОИЗВОДНЫЕ N-АЛКИЛИРОВАННЫЕ ТИАЗОЛИДИНДИОНЫ В КАЧЕСТВЕ МОДУЛЯТОРОВ ЭСТРОГЕН-РОДСТВЕННЫХ РЕЦЕПТОРОВ α
EP2134698A1 (fr) 2007-03-07 2009-12-23 Janssen Pharmaceutica, N.V. Phénoxy aminothiazolones substituées comme modulateurs de récepteur alpha d' strogène
US20090023158A1 (en) * 2007-07-13 2009-01-22 Elan Pharmaceuticals, Inc. Compositions and Methods for Identifying Substrate Specificity of Inhibitors of Gamma Secretase
ES2395083T3 (es) 2007-10-17 2013-02-08 Janssen Pharmaceutica, N.V. Ácidos bifenilcarboxílicos y sus derivados
BRPI0817433A2 (pt) 2007-10-19 2015-06-16 Janssen Pharmaceutica Nv Moduladores de gama-secretase piperidinila e piperazinila
EA017907B1 (ru) 2007-10-19 2013-04-30 Янссен Фармацевтика Н.В. МОДУЛЯТОРЫ γ-СЕКРЕТАЗЫ С АМИДНЫМИ СВЯЗЯМИ
JP5417334B2 (ja) 2007-10-19 2014-02-12 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ 炭素結合したγ−セクレターゼの調節物質
WO2009052350A1 (fr) 2007-10-19 2009-04-23 Janssen Pharmaceutica, N.V. Modulateurs de la g-secrétase liés à une amine
US7968725B2 (en) 2008-07-22 2011-06-28 Janssen Pharmaceutica N.V. Pyridinyl modulators of γ-secretase
US9023767B2 (en) 2009-05-07 2015-05-05 Memorial Sloan-Kettering Cancer Center γ-Secretase substrates and methods of use
CN103502466A (zh) * 2010-09-07 2014-01-08 斯隆－凯特林纪念癌症中心 用于γ-分泌酶测定的方法和组合物
WO2014062771A1 (fr) * 2012-10-16 2014-04-24 The Johns Hopkins University Reconstitution inductible et système cinétique quantitatif en temps réel pour l'étude d'enzymes intramembranaires

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4589297A (en) * 1996-10-07 1998-05-05 Scios Inc. Method to identify direct inhibitors of the beta-amyloid forming enzyme gamma-secretase
DE19941039A1 (de) * 1999-08-28 2001-03-01 Boehringer Ingelheim Pharma gamma-Sekretase in vitro Testsystem

Also Published As

Publication number Publication date
CA2452832A1 (fr) 2003-01-30
MXPA03011898A (es) 2004-03-26
WO2003008635A3 (fr) 2004-01-29
WO2003008635A2 (fr) 2003-01-30
EP1407043A2 (fr) 2004-04-14
JP2004535206A (ja) 2004-11-25
WO2003008635A8 (fr) 2003-10-30

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Legal Events

Date Code Title Description
8127 New person/name/address of the applicant

Owner name: BOEHRINGER INGELHEIM PHARMA GMBH & CO.KG, 55218 IN

8139 Disposal/non-payment of the annual fee