DD299415A5 - DISINFECTANT SUBSTANCE FOR RENOVATION OF PEEL - Google Patents

Abstract

The invention relates to a disinfectant solution for germ removal and for disinfecting cleaning. Oxyethylated sulfobetaines of the formulas (I) and (II) in the chain length range of C12 to C18 are used, which are significantly synergistically enhanced by alcohol in its disinfecting action. According to the invention, a comprehensive germ-reducing effect against viruses, bacteria, fungi, parasitic protozoa and helmints is achieved, which can be used both for skin disinfection and for disinfecting smooth and rough surfaces. In contrast to peracetic acid there is no weakening by blood. Conventional disinfectants with acidic, oxidative, irritating, sensitizing or corrosive properties can be replaced. Formulas (I) and (II) {Degradation; Disinfection; disinfecting cleaning; synergistic effect; sulfobetaines; sulfone; viruses; bacteria; mushrooms; parasitic protozoa}

Description

(CH ₉ CH ₉ O) _T , H (CH ₉ CH ₉ O) H SO ₉ Ma

ct ct111Ct CtJLIct

(I) (ID

in which R is alkyl radicals having 12 to 18 carbon atoms, η and m are numbers from 3 to 7, which can be used in water in concentrations of 0.01 to 2 wt .-% or for synergistic increase in activity in the same concentration in water / Alcohol mixtures with a share of 20 to 99 vol .-% water and 1 to 80 vol .-% alcohol are dissolved.

2. disinfectant solution according to claim 1, characterized in that ethanol is used as the alcohol or a propanol.

3. disinfectant solution according to claim 1 and 2, characterized in that for skin disinfection with specific activity against hepatitis B virus, the active compounds of the formulas (I) and (II) in water / alcohol mixtures having an alcohol content of 30 to 80 vol. -% to be solved.

4. disinfectant solution according to claim 1, characterized in that for the complete dissolution of blood contaminants, the active compounds of the formulas (I) and (II) are used in the concentration range of 0.1 to 2 wt .-%.

Field of application of the invention

The application relates to the killing / inactivation of germs with a broad effect in the human and veterinary field as well as in the food industry.

The application is possible for facilities where measures for a multivalent germ reduction, a germ-reducing cleaning or disinfection are necessary.

Characteristic of the known state of the art

Multivalent agents for killing germs, which at the same time are characterized by user-friendliness, high efficiency as well as toxicological and ecological criteria, take into account the capillary problem and which are not least economical to produce, do not yet exist. Due to the diversity of germs, the fields of application, products and processes or the compatibility and environmental impact of disinfectants, the area is very confusing and in need of improvement. A classification according to chemical substance classes with reference to the corresponding fields of application is also difficult. On the other hand, because of the diversity of the germs, the disinfectants used are also kept variable, and the chemical agents are often combined or used together with physical means (eg, temperature increase) for use.

Because of the versatility of the application criteria, therefore, the individual disinfectants are adapted for the individual disinfection procedures and especially disinfection tasks (hand, skin, instrument disinfection, etc.). The demands for faster and safer disinfection are difficult to reconcile with the requirement for compatibility, the requirement not corrosive effect and other environmental and economic aspects such as durability, shelf life, odor, skin irritation and handling. According to the state of the art, high efficiency and safety are accompanied by disadvantages in handling. In the search for new, safe disinfectants antiviral agents have not been dealt with comprehensively enough, whereby the above-mentioned disadvantages for virus disinfection exist in a special way and the range of usable antiviral disinfectants is still small. In chemical germ killing the following means have been used so far (HORN, PRIVORA, WEUFFEN, "Handbook of Disinfection and Sterilization" Volumes 1 to 5, Verlag Volk und Gesundheit 1972 to 1984):

- aldehydes or mixtures of aldehydes;

Alcohols, especially ethanol and the propanols;

- halogen compounds, especially iodine compounds and hypochlorites;

- lactones;

- metal compounds (mercuric chloride, copper sulfate);

- Oxydants (peracetic acid, ozone), epoxides;

Phenols, xylenols;

- Quaternary ammonium compounds;

- special surfactants, eg. Carbobetalne,

where new types of disinfectant have not yet prevailed.

Of the compounds mentioned z. As peracetic acid and aldehydes a very wide range of action. However, these substances are usually toxic, unstable in storage and / or have high corrosivity. In addition, there is a rapid inactivation of peracetic acid by blood, which leads to a significant effect restriction. Due to its sensitizing effect, formaldehyde leads to occupational diseases. Spore killing preparations such as performic acid and ethylene oxide are considered to be particularly toxic, e.g. classified as carcinogenic. Phenols have clear gaps in the spectrum of action and are usually unpleasant odor-intensive. Hypochlorites are used despite the considerable user-friendliness. The effect of alcohols against hepadnaviruses can not be proven with certainty, therefore they are used in other risk areas. There are also gaps in the effectiveness of the cationic surfactants and carbobetaines used hitherto.

Effect against selected viruses has been demonstrated for:

- aldehydes (formaldehyde, glutaraldehyde);

- alcohols (ethanol, propanol);

- halogen compounds (especially hypochlorites); .

- peracetic acid;

- some zwitterionic detergents.

In the evaluation of germicide disinfectants, viruses and bacterial spores are a major criterion.

As test viruses have been used very often:

Influenza viruses, vaccinia viruses, poliomyelitis viruses;

then follow:

Coxsackieviruses, ECHO viruses, adenoviruses, herpesviruses.

Influenza and vaccinia viruses are considered to be about the same in their behavior towards disinfectants-despite e.g.

different ether resistance.

Hepadnaviruses are considered to be particularly resistant.

Object of the invention

Z \ e \ of the invention are those disinfectant solutions that allow a multivalent germ killing on chemical or physicochemical wave in accordance with application criteria, at the same time directed against Bacte: ien, fungi and viruses and against other germs, with adaptation to different conditions of use safe germ killing is achieved and a toxic damage is counteracted by the user.

Explanation of the essence of the invention

The object, which is solved by the invention, is to find such disinfectant solutions by which a multivalent germ killing / inactivation of germs without reactivation of the germs is achieved. This disinfectant solution is designed for

high activity against viruses, bacteria, spores and worm eggs;

- ease of use (little or no skin irritant, no corrosion effect, some cleaning effect, low odor, water solubility); >

- favorable characteristics (toxicological, carcinogenic, mutagenic, teratogenic and ecological criteria);

- Consideration of the capillary problem or penetration into pores or rough surfaces;

- dirt dissolution; Dissolution of blood admixtures;

- economic and raw material-side producibility.

The object is achieved according to the invention in that the germ reduction / disinfection by ethoxylated sulfobetaines of the formulas (I) and (II),

(CH ₂ CF ₂ O) _n H (CH ₂ CH ₂ O) _n H

_R __ <% _ CH ₂ -CH ₂ -CH ₂ -SO ₃ R ⁹ ^ ⁹ CH ₂ CH ₂ -SO-CH ₃ ⁰

(CH ₂ CH ₂ O) _m H (CK ₂ CH ₂ O) _{1 N} H SO ₂ Na

in which R is alkyl radicals having 12 to 18 carbon atoms and η and m numbers from 3 to 7, which can be obtained as mixtures according to known simple procedures in mixing ratios of 20: 1 to 1: 1 (DD-PS 154443, DD PS 259851, DD-PS 259852), in aqueous solutions in total concentrations between 0.01 to 2 wt.%, Which may contain ethanol or a propanol for the synergistic increase in activity. In the concentration range of 0.1 to 2% by weight of active compound, complete dissolution of blood contaminants is achieved.

Surprisingly, it has been found that even aqueous solutions in low concentrations bring about a strong inactivation of various germs, while highly resistant germs (eg hepatitis B viruses) are inactivated by the combination of the polyfunctional sulfobetaines with alcohols (ethanol, propanols); Such a synergistic effect-increasing effect occurs according to the invention in solutions containing 20 to 99% by volume of water and 1 to 80% by volume of alcohol. Against hepatitis B virus 30 to 80 vol .-% ethanol or propanol are used in combination with the sulfobetaines according to the invention. This convenient Kombinierbarkelt it is possible to adapt the disinfectant solution for multivalent germ killing various application conditions. This also makes it possible to achieve specific effects against bacteria, spores, fungi, viruses, parasitic protozoa and worm eggs.

The disinfectant solution according to the invention is suitable for skin disinfection as well as for reducing the germ on hard or rough surfaces, for example devices, painted surfaces, metals, enamels and ceramics. It does not irritate the skin, does not corrode, does not show an unpleasant odor, is non-toxic and can be stored indefinitely as a substance as well as in solution. If necessary, it can be combined with other aqueous or aqueous-alcoholic agents. By the aforementioned property image, it is superior to conventional disinfectant solutions in effect and use value and can be produced inexpensively.

embodiments example 1 To a microorganism mixture with 10 "germs / ml in strongly viscous nutrient medium from Bacillus subtilis, Endomycopsis

bispora including their spores, a 4: 1 mixture of the ethoxylated sulfobetaines of formulas (I) and (II), prepared according to DD-PS 259851, in a final concentration of 0.2 wt .-% mixed and incubated for 30 minutes. After deactivation of the active ingredient by dilution with sterile tap water, the test for sterility with the nutrient media is carried out according to

Pharmacopoeia DDR, Volume I, X, 4.0.1.ff. und7.0.1.ff. Results:

- with the active substances of the formulas (I), and (II): sterile

- Control without active substance: germ growth

Example 2 To chicken blood containing 10% by volume citrate buffer, influenza virus type A strain A / PR / 8/34 in allantoic fluid

given. After thorough mixing, such a volume of a 20% solution of a 1% mixture of deoxyethylated sulfobetaines of the formulas (I) and (II), prepared according to DD-PS 259 851, in 10% ethanol, is added

Final concentration of active ingredient 0.25 wt .-% is. After renewed mixing and a contact time of 10 minutes

is deactivated by the addition of at least 100 times the volume of a buffered NaCl solution.

Thus, pre-incubated chicken eggs are inoculated according to the usual methods for the propagation of influenza viruses. Of these Eggs are taken sterile after an incubation period of at least 48 hours AllantoisflüssigkeK and the Virus multiplication compared to control inoculated with NaCl solution and to the hatching eggs without active ingredient by means of the Hemagglutination test comparatively assessed. Results:

- With active substance mixture of the formulas (I) and (II): no virus multiplication

- without active substance: full virus replication

Inactivation testing was performed according to WHO requirements for Influenza Vaccine (Inactivated). Example 3 To a buffered virus suspension containing at least 10 ⁴ infectious units per ml of adenovirus (human) type 2

such a volume of a 10% solution of a 4: 1 mixture of the ethoxylated sulfobetaines of the formulas (I) and (II), prepared according to DD-PS 259851, in sterile tap water that the final concentration of active ingredient is 0.4% ,

After mixing and a contact time of 30 minutes, add at least 10 times the volume

deactivates a lactalbumin-containing phosphate buffer solution.

Thus, a permanent cell line suitable for the propagation of these adenoviruses is inoculated by the usual methods. From

each culture vessel is sub-cultured and the virus replication compared to the unvaccinated control and the cell cultures with adenovirus without drug using the cytopathic effect (CPE) as well as the immunofluorescence comparative evaluated.

Results:

- with active substance mixture of the formulas (I) and (II): no CPE

- without active substance: CPE

Example 4 Inactivation of hepatitis B surface antigen (HBsAg) in the suspension test: Equal parts of a HBsAg-containing serum pool in various dilutions and the disinfectant to be tested

be mixed. After a reaction time of 30 minutes, the samples are inactivated by a dilution of 1: 100.

The residual activity of HBsAG is determined by radioimmunoassay compared to a control batch.

rest control activity approach = 100% serum pool serum pool neat. 1:60 0.1% active ingredient of the formulas (I) and (II) in 70% ethanol (1:11 = 4: 1) 10% 8th% 0.1% in water 90% 65%

Example 5

Mäuseschwänzchentest:

10mm long tail pieces are inserted after thorough pre-cleaning for 5 minutes in a HBsAg-containing serum pool and dried for 30 minutes at room temperature. For disinfecting the tail pieces are individually in 50-ml measuring cup, the bottom of which is covered with glass beads, inserted, 1 ml disinfectant solution (0.1% solution of ethoxylated Sulfobetalngemisches of formulas (I) and (II) In 50% lgem n Propanol) was added and shaken for 2 or 30 minutes. Thereafter, the sample is diluted 1:50 and the residual activity of HBsAg determined by radioimmunoassay. The tail pieces are doused with 3 ml of physiological saline and shaken again for 5 minutes. The residual activity in the rinsing liquid is again determined by a radioimmunoassay.

Results:

Residual activity of HBsAg; (Control = 100%)

Exposure time 2 min exposure time 30 min

Disinfection, rinsing, disinfection, rinsing

Solution Solution Solution Solution

9% 2 ^ 5% 6% 1%

Example β

Guinea pig skin test

Furred out of shaved dorsal skin of guinea pigs pieces of fur are placed around buttons with a flat surface (diameter 2.5 cm), sutured on the back with surgical suture and fixed by inserted into the buttonholes wooden sticks. For each test, 2 germ carriers prepared in this way are required.

A germ carrier is charged with 0.05 ml HBsAg-containing serum and dried for 30 minutes at room temperature. Then we added 0.5 ml of the disinfectant solution to be tested and carried out with the help of the second germ carrier washing movements. After a contact time of 2 or 30 minutes, the disinfectant solution is washed off and then rinsed the germ carriers for 5 minutes with 3 ml of 0.9% saline. The residual activity of HBsAg was determined by radioimmunoassay.

Results:

0.1% solution of a 1: 1 mixture of ethoxylated sulfobetaines of the formulas (I) and (II) in 90% n-propanol; Residual activity of HBsAg.

Control substance = 100%>

Exposure time 2 min exposure time 30 min

6% 2%

Claims (1)

1. Desinfectant solution for germ killing, characterized in that the germ reduction, germ-reducing purification and / or disinfection by ethoxylated sulfobetaines of the formulas (I) and (II) (CHoCHpO) _n H (CHoCHoO) H ιCt C *ti ιL · Lt \\ tv '"'" N L «rlo OHq vHn" Ονο Iv "" N '* ~ "ΟΗο V ^ H νΠο * ~~ ονο