DD246471B1 - MEDIUM FOR LUBRICATION AND LIQUID CONSERVATION OF EBERSPERMA - Google Patents

Description

ad 1 000ml water redist. with the addition of 0.5g streptomycin sulfate and 500000IE penicillin G

field of use

The invention relates to a more effective and economical means of diluting and preserving boar semen in liquid form at 15-17 ° C, which is used for the artificial insemination of sows.

Characteristic of the known solution

For storage of boar semen from extraction to insemination, the ejaculates are mixed with a dilution medium and cooled to 15 ° C and stored. As preservative media, various modifications of glucose-sodium citrate diluents, such as. B. the IVT medium used. Increasingly, a returning on Pliško EDTA-containing medium is used (Invention description SU 183901}. Using this Pliško Thinner the boar semen can be applied to 3 χ 10 ^э forward motile sperm per insemination dose of 100 ml, single-stage, stored at 15-17 ⁰ C and up to An extension of the storage period of the fluid-conserved boar semen to 50 h was achieved by the introduction of a two-stage preservation method (animal breeding 78, 1974, 543-544), whereby a modified AEDTA medium is used for the first dilution stage. The second diluent is a modified IVT diluent with elevated pH, which reactivates the sperm prior to insemination.

The requirement for a storage period of liquid-conserved boar semen up to 80 hours for more effective design of the process and saving of energy could not be met with this process variant. Even on an international scale, liquid preserved boar semen is used only for up to 50 hours without waste of fertilization results.

Object of the invention

The viability and vitality of the boar sperm should be improved in order to ensure high fertilization results up to a storage time of 80 hours. The 80-hour duration of use of the preserved semen makes it possible to rationalize the biotechnical process of artificial insemination in pigs through more effective work in the insemination centers and in the sowing farms, as well as a reduction in carburettor fuel by optimizing the transport of semen.

Explanation of the essence of the invention

The object of the invention was to develop a medium for dilution and liquid preservation of boar semen, which delays the aging processes occurring in the sperm during storage so that an 80-hour use of the liquid-conserved sperm without reducing the fertility of the sperm is possible. For this it was necessary to stabilize the sperm membrane, so that their functionality is maintained longer. This was achieved by adding the SH-group carrier cysteine and by optimizing the osmotic conditions in the dilution stream by changing the amount of the known thinner components. A medium of the following composition meets the requirements (g / l):

Glucoses (C ₆ H ₁₂ O ₆ ) 36.0 to 44, Og Sodium Citrate (Na ₃ C ₆ H ₅ O ₇ · 2 H ₂ O) 3.3-4.1 g Sodium bicarbonate (NaHCO ₃ ) 1,1-1,3g Chelaplex III (C ₁₀ H ₁₄ O ₈ N ₂ Na ₂ 2 H ₂ O) 1.8-2.2 g L-cysteine hydrochloride (C ₃ H ₇ NO ₂ SHCl · H ₂ O) 0.04-0.06 g

The diluent and preservative according to the invention is used as a first diluent with the addition of 500000 IU of penicillin G and 0.5 g of streptomycin sulfate as a germinating agent in a two-step procedure and thus allows the use of the preserved sperm up to 80 hours.

embodiments example 1

The following variants of the dilution medium were tested in vitro for their effect on the survival and resistance of sperm and the maintenance of their normal acrosome morphology (NAR):

Substance (g / l) I Il III glucose 36.0 40.0 44.0 sodium citrate 3.3 3.7 4.1 Chelaplexlll 1.8 2.0 2.2 sodium bicarbonate 1.1 1.2 1.3 L-cysteine hydrochloride 0.04 0.05 0.06

With these compositions, the following survival rates (HP) and thermoresistance (TRT) - and NAR values are stored after 80 hours storage of the preserved sperm in comparison to the old diluent - 50 h:

criteria 0 variants Il 41.7 III older 60 I of the new thinner thinner 120 45.0 33.3 23.7 HP 180 (Share forward 240 91.5 Portable 300 36.7 Sperm%) χ 0-300 91.7 37.5 89.3 89.7 NAR% 35.8 40.0 40.0 25.4 TRT 35.0 34.2 50.0 31.9 (Share of 36.7 25.8 42.5 31.4 wärtsbewegl. 33.3 22.5 36.7 30.5 Sperm after 29.2 32.8 27.5 25.3 ... min Inkuba 25.8 23.3 25.3 tion at 40 ⁰ C 32.6 32.5 28.7

Example 2

Preferably, the following formulation is used for the medium:

glucose 40.0 g sodium citrate 3.7 g Chelaplexlll 2.0 g sodium bicarbonate 1.2g L-cysteine hydrochloride 0.05 g

The thinner components are redistilled in Aqua. of 35-40 ⁰ C dissolved in 1000 ml, 0.5 g of streptomycin sulfate and 500000IE of penicillin G are added. This preservative is added to the original filtered boar semen in an amount containing 6 x 10 ⁹ sperm per 100 ml of forward motile sperm, ensuring equality of thinner and sperm. This diluted semen is stored at 15-17 ° C. Before insemination, 50 ml of the diluted sperm are mixed with 50 ml of secondary diluent and within 80 hours sperm collected. The semen conserved in this way shows, compared to the thinner used up to now, better sperm survival rates as well as fertilization results stored up to 30 h:

older new thinner thinner Proportion of forward mobile Sperm after 30 h 54% 50 h 48% 56% 80 h 52% (= HP of 220 ejaculates) until 30 h 50-8Oh Number of first inseminations 970 972 Pregnancy rate (%) 84.7 82.4 live born piglet / litter 10,80 10.79

Claims (1)

Composition for dilution and liquid preservation of boar semen consisting of glucose, sodium citrate, sodium bicarbonate, chelaplex IM and L-cysteine hydrochloride and the addition of antibiotics, characterized in that the individual constituents are present in the following concentrations: Glucoses (C ₆ H ₁₂ Oe) 36.0-44, Og Sodium citrate (Ma ₃ C ₆ H ₅ O ₇ .2H ₂ O) 3.3-4.1 g Dinatriumdihydrogenäthylendiamin- tetraacetate (C ₁₀ H ₁₄ O ₈ N ₂ Na ₂ .2H ₂ O) 1.8-2.2 g Sodium bicarbonate (NaHCO ₃ ) 1.1-1.3 g L-cysteine hydrochloride (C ₃ H ₇ NO ₂ SHCl-H ₂ O) 0.04-0.06 g