DD200372A3 - METHOD FOR OBTAINING GREEK AND TRACE-LIKE BIOMASSES - Google Patents

Abstract

The invention relates to a method for obtaining biomass with a high crude protein and trace element content in the field of biotechnology. The object of the invention is to present a process for obtaining biomass with a higher crude protein and trace element content than in processes based on conventional raw materials. An increase in the utility value of the biomass produced compared to biomass grown on conventional substrates is observed. The invention has for its object to introduce the inorganic salts of the nutrient and Spurensalzmediums at an optimal pH in the fermentation process so that the physicochemical properties of the nutrient and Spurensalzmediums avoid increased technical and economic effort and the inorganic components in sufficient extent for the Utilisierungsprozess in dissolved form are available. According to the invention the object is achieved by the inorganic salts in the form of two nutrient solutions, an aqueous potassium chloride solution and an acidic salt solution consisting of magnesium, copper, -. Manganese and manganese sulfate and ferric chloride or ferrous sulfate, phosphoric acid and water, is fed to the fermentation process. The pH of the fermentation medium is between 2.0 to 4.5. The biomass obtained after a conventional work-up is rich in protein and contains a high mineral content, in particular magnesium, copper, iron, manganese and zinc. The utility value as a feed component in animal nutrition is thereby improved.

Description

233

title

Process for the recovery of biomass rich in protein and trace elements ·

Application Born of the ina.ung

The invention relates to a method for obtaining protein-rich and trace element-rich biomasses, which are by a microbial conversion of hydrocarbon mixtures ^ especially petroleum distillates or »raffinates with a n ^ carbon chain length range from Cg to C ^ ₀ , with yeasts beech« It can be in the microbiological Industri and is classified in the IPK C 12 Ή »

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Egg v / eiBreiehe microbial biomass which _ö B are based on hydrocarbon "mixtures, in particular n-alkane-containing Ei'döldes'tillaten BZV / _e -raffinaten of the carbon chain length range Cq to C-5Q obtained by culturing microorganisms, such _o , are known to be produced in the fermentation process. "The process is a biochemical, heterogeneous, exothermic reaction in which several partial reactions take place side by side." Four phases, cell sub- stance j aqueous nutrient medium, hydrocarbon mixture and atmospheric oxygen are involved in this. The rate of mass transitions depends on the degree of distribution of the phases in the per- mentor and on the reaction conditions used

Such methods _are? DE-OS 105 825 set forth in DE-OS '1 417 "56o, DE-OS 1 470 484, DE-DAS 1 470 484, DE-OS 1 470 49o 1 470 517 and DD-WP,.. In the above methods, the intensity of the wax

  5 trims of the microorganisms used as production strains strongly influenced by the pptimal composition of the nutrient and trace brine media used and the pH value. "In the known breeding processes for biomass production. The inorganic salts added as nutrient and trace salts are added to the reactor in dissolved porin. For this purpose, the solid salts are dissolved in solvent or "chemical stations" according to specific working instructions and formulations and stored as solutions in storage containers before they are used for the process to be provided*

It is also a method known "where the inorganic salts are introduced in solid form or as mash directly into the per-, mentor and / or in the permentor 'leading streams * The salts are then dissolved by applying certain conditions in the reaction medium (DD -WP 111 584) * DD-WP 105 001 describes a method for obtaining Hefezellsubstanz with predetermined value levels on carbohydrates, where during the continuous breeding process per kilogram of zu-winning yeast in the nutrient solutions, the concentrations of iron, copper, zinc and manganese adjusted to provide a high protein content for zinc in the range of 50 to 150 mg, for copper in the range of 22 to 35 mg, for manganese in the range of 30 to 40 mg and for iron in the range of 30 to 40 20 mg- and to obtain a high glycogen content for zinc in the range of 5 to 10 mg, for copper in the range of 3 to 5 mg, for manganese in the area from 10 to 15 mg and for iron in the range of 150 to 200 mg. "These known processes with different regimes of nutrient and trace salt dosage and the production of biologically active

35. masses in high yield and quality have the disadvantage that the stability of the targeted biomass yield and

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the biomass quality in the continuous process is limited *

The use of high-performance piduction stems requires the use of a nutrient and pesticide. Trace salt medium with a

.5 complex composition »When using hydrocarbon mixed, zeolite ₀ n-alkane petroleum distillates or« raffinates in the carbon chain length range C "to C ^ ₀ as carbonaceous source and yeasts as production strains, the culture medium contains the cations K ⁺ , Mg ⁺ _s Cu ⁺ , Pe ⁺⁺⁺ resp. «Pe ⁺⁺ , the anions Cl", SO ^, ~ "% PO. *""""" The underlying inorganic salts are dissolved in an acidic medium "Due to the acidic character of the nutrient solution and the complex Composition of the cations and anions are very demanding on the corrosion resistance of the materials used for containers and pipelines and there is the danger that under the specific conditions of storage of the complex compound nutrient and trace salt medium or * microbial process, precipitates which form sparingly soluble sediments, which are no longer available for biomass synthesis and thus adversely affect the applied V experience in terms of yield and quality of biomass influence *

Object of the invention

The object of the invention is to design a method for obtaining biomass with high yield and quality by a rational and optimal introduction of nutrient and trace salt solution in the fermentation process.

The invention is based on the object, the inorganic salts of nutrient and Spurensalzme'dium'sbei optimal. Introduce pH value in the process of fermentation so that the nutrient and trace salts are fully utilized in the process of perforation and no major saline precipitation occurs, which causes an increased technical and economic effort <5

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According to the invention the problem is solved so that the inorganic salts different in the form of two cooperating "'set nutrient solutions are fed to the fermentation process _β

- 5 The nutrient solution I is a potassium chloride solution. For its preparation, a certain amount of potassium chloride is introduced into 40 to 50 ° C. warm drinking water. After the stirring process has ended, the settled insoluble constituents are separated off as required and the solution is stored until use "the nutrient solution II is 40 ° C, warmed from the salts magnesium sulfate, copper sulfate, zinc sulfate, ferric chloride, phosphoric acid and water existing acid salt solution" drinking water for this purpose is followed by the specified _e first the phosphoric acid, the ferric chloride solution and then the other salts in the stirred vessel used. "Stirring is started after the addition of the liquid products and continued for at least 15 minutes after adding all the chemicals. Afterwards, the still missing warmed-up drinking water is added."

The nutrient and trace salt solutions for the fermentation process of hydrocarbon mixtures, in particular petroleum distillates and / or raffinates with yeast, are selected on the basis of the specific consumption figures of the fermentation process.

The dosage of nutrients and trace elements resulting from the specific consumption and process values must be adjusted to the actual productivity achieved in the continuous process *. ·

In addition, it has been found that in the pH range of 2.0 to 4.5 the viability of the yeasts. Stability of the nutrient medium is ensured and the occurrence of foreign infections is limited "Below pH = 3 the growth of the microorganisms is reduced ₅ their viability is maintained" A pH of 4.1 - 0.1 was found to be more optimal as a result of many years of experimentation pH "value found" Above pH = 4s5, the stability of the nutrient medium is not

/ 4

longer guaranteed _$ da by \ '. drd the wax turn SBu schwindiglrteit reduced * In the continuous process, a flushing of the biological substance is a consequence, the risk of foreign infection increases * The addition of two specific .zusammengesetzten Nutrient Solutions in the Permentationsprozeß in corrosion> plunging with an optimum pH at Eineatz a petroleum distillate or raffinate in the carbon chain length range of C ₀ to · CUq as a carbon source and the yeast iodderomyces elongispor-us as a production strain results in a biomass, in the form of its dry product, the following average composition in terms of crude protein content and trace element content. has:

Crude protein content: 62 _s 7 %

Magnesium: 1 400 mg / kg

Copper: 75 _s 5 mg / kg

"Iron ^:? · 484 mg / kg manganese: 149 mg / kg

Zinc: 725 mg / kg

This prerequisites for effective storage of! Annually and trace elements are created _$ of the products contained in selected ='s balanced biological ratio in the fodder ~ gen _e As shown in the table below, the biomass materials based on hydrocarbon "* and yeast differs compared to biomass based on conventional substrates and yeasts in the trace element content, especially in the zinc content, but also the manganese, copper "and iron content is proven advantageous" With an optimal breeding regime and favorable nutrient and

3'0 Spurensalzbedingungen and an optimal pH value it comes thus to storage urge effects for certain trace elements by the microorganism *

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 table

Trace elements "of feed yeast

Q ₁₁ V ₀₊ ^ ₀ J. Micronutrient amount (mg / kg feed yeast) _{Mg Cu pe Mn Zn}

Petroleum distillate 1400 75.5 484 149 725 (C ₀ to C, _Λ ) ι

Sulfite waste liquor (Pichte) , 2300 8th 354 63 110 Sulfite waste liquor 10 (beech) 1650 5 250 97 45 • molasses / vinasse 1000 77 662 100 105 molasses 1560 12 575 41 120 Raw cane sugar / molasses 2190 32 212 42 150

The invention is explained in more detail in the following example

In a stirred fermentor with a total volume of 250 m and a working volume of 90 t, yeasts of the strain Lodderomyces elongisporus on a petroleum distillate fraction of. boiling range

, The n-alkane content in the petroleum distillate is 16.5% "The fermentation temperature is 33 ° C, the pH is 4 _S 2 * The specific fumigation intensity is 6500 m / h, the energy consumption 250 kW * The residence time is 5 hours. The nutrient and trace salts are introduced into the fermentation process in the form of two nutrient salt solutions. Nutrient salt. Solution I is a 15% aqueous potassium chloride solution, nutr. Salt solution II is an acidic salt solution consisting of the following media: magnesium sulphate, copper sulphate, zinc sulphate, manganese sulphate, ferric chloride, phosphoric acid and water, resulting from the quantitative and quantitative quantities of nutrients and trace elements given below of the continuous process.

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Specific consumption indices of nutrient and trace salt solutions

element speze D needs inserted g E / kg HTS · raw material 5 p 17.5 ~ 18 Phosphoric acid, 74.5 % ± g K 20 potassium chloride mg O _f 94 'Magnesium sulfate Pe 0.02 Ferrous sulfate Cu 0.02 - 0.03 copper sulphate 10 mn 0.16 manganese sulfate Zn 0.22 zinc sulfate

The nutrient solution II has the following composition based on anhydrous substance *

'Medium • 7 H ₂ O Mass ~% 15 _MgSO4 • 5 HPO 10.20 CuSO ₄ * 7 H ₂ O 0.18 PeSO ₄ * 7 H ₂ O 0.18 ZnSO ₄ - 4 H ₂ O 0.69 MnSO ₄ (74, , 5% ± s). 0.58 20 H ₃ PO ₄ Drinking water 10.17 78,00

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An excipient combination of the structure propylene oxide ethylene oxide adduct / polyethylene glycol in a concentration of 600 mg / 1 aqueous culture medium is added to the aqueous culture medium in order to intensify the process of perforation.

The productivity of the fermentation process is 3 * 6 kg. • Yeast dry substance / t

In a separating apparatus, a separation of 60 % of the process water from the four-phase mixture of petroleum distillate / biomass / process water / air is achieved by 'framing *. The distilled petroleum distillate / biomass / process water suspension is reduced by approx -

combination with and heats the suspension to 80 to 85 ° C. * * * * * * * * * The dewaxed petroleum distillate is separated by means of two industrial separators and further process water. The biomass suspension concentrated to about 10.5% is further enriched by vacuum evaporation. and then in a Sprühtrocknungsprο ζ dried ESS The process water from creaming and 40% of the separated aqueous phase in the separation be in the Pennentationsprozeß recycled "

By extractive cleaning of dry biomass in a continuous. Rotary disk extraction unit at 40 ° C with an extractant mixture gasoline-ethanol-water (80-19% Vo1%) removes residual hydrocarbons and most of the lipids from the biomass * The extractant is then removed from the extracted biomass by a two-stage thermal drying The end product obtained is characterized by high quality, optimal digestibility and high biological value. «

Appearance; beige to light brown

Stocking: without

Qefüge: not lumpy

Odor; typical crude protein content: 62.6 %

Lysine content: 1.5% ,

Magnesium: 1438 mg / kg

Copper: 83 mg / kg

Iron: 489 mg / kg

Manganese:. 164 mg / kg

Zinc: 765 mg / kg

Claims (1)

0% 1 "process for obtaining protein and trace elements" · rich biomasses on by growing yeast Coal mixtures, in particular petroleum distillate bzv / e raffinates having a colloidal fluid range Cq to C ^ ₀ are obtained, characterized in that the dosing of the inorganic nutrient and lane saliva in the form of two differently composed Na'hrsalzlösungen in''Permentationsproaeß ., Where the nutrient solution I contains a 10 to 20%, in particular ca, 15% strength aqueous potassium chloride solution, the nutrient solution II contains one of the salts magnesium sulfate, copper sulfate, zinc sulfate, manganese sulfate, iron. chloride salt, ferric sulfate, phosphoric acid and water, the quantitative composition of which depends on the specific requirements of the fermentation process at a pH of from 2.0 to 4.5, in particular 4 , 1 ~ 0.1 of the JP fermentation medium