DD137383B1 - METHOD FOR STABILIZING THE KEIMRELATION IN BACTERIALLY INFECTED URINE FOR SEVERAL DAYS - Google Patents

Description

Field of application of the invention

The invention relates to a method for transporting examination material, preferably urine, into a microbiological laboratory for further processing of bacterially infected urines.

Characteristic of the known technical solutions

It is already known that a colloidal system consisting essentially of polymeric carbohydrates, inorganic swelling stents and phosphate buffer and a redox indicator with practically 100% sensitivity at over 90% specificity, for the differential diagnostic clarification of acute and chronic recurrent Hamwegsinfektionen - DO -PS 10175Э. in the case of a positive reaction, indicated by a more or less strong reduction of the indicator, there is a need to send another urine sample to a corresponding microbiological laboratory to identify the germs causing the disease, including the determination of their chemotherapeutic susceptibility.

Prerequisite, however, is that the transport 2-3Sid. does not exceed. Such a requirement can not be realized in practice or only with great effort.

In the case of a positive reaction, a further subset of the urine sample, which was branched off from the initial determination and stored accordingly, is inoculated into a nutrient medium suitable for the growth of the pathogens within the specified period of time.

For vaccination, a number of suitable, albeit relatively expensive, agents have been proposed, such as the most widely used culture media coated slides, or sparsely cast media in certain tubes, and other developments based essentially on the same principle. This process-related process is very time consuming and associated with considerable costs. This method is practically unsuitable for carrying out large-scale screening in a routine laboratory. In addition, chemical preservatives have been proposed to prevent quantitative and qualitative changes in the germ content in the urine during transport, containing as active substances glycerol, merthiolate, ethylenediamine tetraacetic acid, polyvinylpyrrolidone, paraaminobenzoic acid, boric acid and dimethyl sulfoxide and partly sinzein, partly in different combinations ,

According to the specialist literature and the agreed opinion of the experts, preservation can only be guaranteed to a varying extent with the help of these substances, albeit not for all types of germs, for up to 48 hours.

Object of the invention

The invention makes it possible to demonstrate a significant bacteriuria in a technically simplest manner, and, moreover, without the risk of a qualitative change in the germ relations in the case of mixed infections, the urine can be sent for further examination in a microbiological laboratory, whereby the previously required chemical preservation is eliminated.

Explanation of the essence of the invention

The invention has for its object to suitably eliminate the additional inoculation of the urine to be examined for a suitable transport medium. According to the invention, it has now been found and proven by experiments that a test reagent can be used as the transport medium for further microbiological examination of a significant bacteriuria which is able to stabilize the bacterial digestion for several days in the case of a bacterial infection of the hamstrings and at the same time to suppress any contamination germs ,

Such a test reagent is, for example, a colloidal system which contains a dye reducible by the bacteria and suitable nutrients using a protective colloid and an added phosphate or citrate buffer substance.

Execution example

This already known test reagent, which is preferably present in tablet form, is first mixed with the material to be investigated, such as urine, or dissolved in the submerged material, whereby according to the invention about 1 ml of urine is mixed with 0.2 g of test reagent.

After incubation at temperatures between 20 to 27 ° C for 16-20 hours. It is judged whether there has been a more or less strong reduction of the indicator used (preferably methylene blue) by the possibly contained in the urine bacteria. If such a reduction is recognizable, a significant bacteriuria can be assumed to be 99-100%, ie more than 10 ^ä germs / ml of pathogenic microorganisms are contained in the urine.

The composition of the test paper is designed in accordance with DD-PS '01759 so that with a specificity of more than 90% only this typical germ count range for urinary tract infections is detected.

The stabilizing effect extends to 3-4 days.

This eliminates any further additional inoculation of the urine to be examined for another, suitable for transport medium or his occupation with a chemical preservative. That is, the the presence eir.er significant bacteriuria indicating more or less strongly reduced urine test tablet approach can be forwarded to the competent bacteriological laboratory directly to the identification of the existing types of germs and the determination of the chemotherapeutic sensitivity in the tightly closed original approach tubes.

This not only results in a considerable increase in the differential diagnostic value of the bacteriuria tablet test, but also the broadly necessary application of this screening test for prophylactic reasons becomes economically feasible.

Claims (1)

-1- 205 178 Invention claim: A method for stabilizing the germination of bacterially infected urine over several days with extensive 'suppression of growth of any existing contamination germs, characterized in that the urine with a known for the cultivation of bacteria, buffered culture medium on a colloidal basis, a reducible by bacteria dye in the order of a few parts of the teeth, based on the nutrient medium, is added.