CZ308509B6 - Způsob přípravy geneticky upravené drůbeže, rezistentní k ptačímu leukózovému viru podskupiny J - Google Patents

Způsob přípravy geneticky upravené drůbeže, rezistentní k ptačímu leukózovému viru podskupiny J Download PDF

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Publication number
CZ308509B6
CZ308509B6 CZ2019-392A CZ2019392A CZ308509B6 CZ 308509 B6 CZ308509 B6 CZ 308509B6 CZ 2019392 A CZ2019392 A CZ 2019392A CZ 308509 B6 CZ308509 B6 CZ 308509B6
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Czechia
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resistant
avian leukosis
leukosis virus
genetically modified
poultry
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CZ2019-392A
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CZ2019392A3 (cs
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Jiří Hejnar
Pavel Trefil
Anna Koslová
Jitka Mucksová
Jiří Plachý
Jiří Kalina
Markéta Reinišová
Dana Kučerová
Original Assignee
Ústav molekulární genetiky AV ČR, v.v.i.
BIOPHARM, Výzkumný ústav biofarmacie a veterinárních léčiv a.s.
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Application filed by Ústav molekulární genetiky AV ČR, v.v.i., BIOPHARM, Výzkumný ústav biofarmacie a veterinárních léčiv a.s. filed Critical Ústav molekulární genetiky AV ČR, v.v.i.
Priority to CZ2019-392A priority Critical patent/CZ308509B6/cs
Priority to CN202080044789.4A priority patent/CN115003155A/zh
Priority to PCT/CZ2020/000030 priority patent/WO2020253894A1/en
Priority to KR1020227000427A priority patent/KR20220018016A/ko
Priority to AU2020297483A priority patent/AU2020297483A1/en
Priority to EP20743052.1A priority patent/EP4075964A1/en
Publication of CZ2019392A3 publication Critical patent/CZ2019392A3/cs
Publication of CZ308509B6 publication Critical patent/CZ308509B6/cs

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    • A—HUMAN NECESSITIES
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2227/30—Bird
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
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Abstract

Řešení se týká způsobu přípravy geneticky upravené drůbeže, rezistentní k ptačímu leukózovému viru podskupiny J, které je charakterizováno tím, že tato drůbež nese deleci W38 zavedenou do genu chNHE1 pomocí homologní rekombinace mediované CRISPR/Cas9, případně že nese deleci W38 zavedenou pomocí CRISPR/Cas9 a specifickou sgRNA, za vzniku kura domácího nebo krocana domácího rezistentního vůči ptačímu leukózovému viru ALV podskupiny J.
CZ2019-392A 2019-06-19 2019-06-19 Způsob přípravy geneticky upravené drůbeže, rezistentní k ptačímu leukózovému viru podskupiny J CZ308509B6 (cs)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CZ2019-392A CZ308509B6 (cs) 2019-06-19 2019-06-19 Způsob přípravy geneticky upravené drůbeže, rezistentní k ptačímu leukózovému viru podskupiny J
CN202080044789.4A CN115003155A (zh) 2019-06-19 2020-06-18 抗j亚群禽白血病病毒转基因家禽的制备方法
PCT/CZ2020/000030 WO2020253894A1 (en) 2019-06-19 2020-06-18 Method of preparing genetically modified poultry resistent to subgroup j avian leukosis virus
KR1020227000427A KR20220018016A (ko) 2019-06-19 2020-06-18 J 아군 조류 백혈병 바이러스에 대한 저항성을 가진 유전자 변형된 가금류를 만드는 방법
AU2020297483A AU2020297483A1 (en) 2019-06-19 2020-06-18 Method of preparing genetically modified poultry resistent to subgroup J avian leukosis virus
EP20743052.1A EP4075964A1 (en) 2019-06-19 2020-06-18 Method of preparing genetically modified poultry resistent to subgroup j avian leukosis virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CZ2019-392A CZ308509B6 (cs) 2019-06-19 2019-06-19 Způsob přípravy geneticky upravené drůbeže, rezistentní k ptačímu leukózovému viru podskupiny J

Publications (2)

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CZ2019392A3 CZ2019392A3 (cs) 2020-10-07
CZ308509B6 true CZ308509B6 (cs) 2020-10-07

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EP (1) EP4075964A1 (cs)
KR (1) KR20220018016A (cs)
CN (1) CN115003155A (cs)
AU (1) AU2020297483A1 (cs)
CZ (1) CZ308509B6 (cs)
WO (1) WO2020253894A1 (cs)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114410808B (zh) * 2022-03-30 2022-07-01 华南农业大学 一种鸡a、k亚群禽白血病遗传抗性分子标记及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ307102B6 (cs) * 2016-06-10 2018-01-10 Biopharm, Výzkumný Ústav Biofarmacie A Veterinárních Léčiv, A.S. Způsob produkce spermií a transgenních ptáků
CZ307285B6 (cs) * 2013-01-28 2018-05-16 Ústav molekulární genetiky AV ČR, v.v.i. Polymorfismy v sekvenci NHE1 kura domácího asociované s rezistencí nebo sníženou senzitivitou k ALV-J

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CN101935675B (zh) * 2010-01-14 2012-06-06 山东农业大学 细胞膜表达ALV-env蛋白荧光真核转基因表达质粒的构建与应用
CN102851355B (zh) * 2012-03-20 2014-12-10 华南农业大学 一种优质鸡抗a亚群禽白血病病毒的抗性分型方法
CN102876675A (zh) * 2012-06-20 2013-01-16 韩健宝 J亚群禽白血病病毒的肽核酸及其应用
CN104152445A (zh) * 2014-07-30 2014-11-19 华南农业大学 鸡b、d、e-亚群禽白血病遗传抗性相关的单核苷酸多态性分子标记及应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ307285B6 (cs) * 2013-01-28 2018-05-16 Ústav molekulární genetiky AV ČR, v.v.i. Polymorfismy v sekvenci NHE1 kura domácího asociované s rezistencí nebo sníženou senzitivitou k ALV-J
CZ307102B6 (cs) * 2016-06-10 2018-01-10 Biopharm, Výzkumný Ústav Biofarmacie A Veterinárních Léčiv, A.S. Způsob produkce spermií a transgenních ptáků

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KOSLOVÁ A. et al.: „Genetic resistance to avian leukosis viruses induced by CRISPR/Cas9 editing of specific receptor genes in chicken cells," Viruses, vol. 10, no. 11, 2018, str. 605, ISSN 1999-4915 *
LEE H. J. et al.: „Precise gene editing of chicken Na+/H+ exchange type 1 (chNHE1) confers resistance to avian leukosis virus subgroup J (ALV-J)," Developmental and Comparative Immunology, vol. 77, 2017, str. 340 – 349, ISSN 0145-305X *
LEE H. J. et al.: „Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B and J," Journal of animal Science and Biotechnology, vol. 10, no. 23, 2.4.2019, ISSN 2049-1891 *

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Publication number Publication date
KR20220018016A (ko) 2022-02-14
CN115003155A (zh) 2022-09-02
WO2020253894A1 (en) 2020-12-24
CZ2019392A3 (cs) 2020-10-07
EP4075964A1 (en) 2022-10-26
AU2020297483A1 (en) 2021-12-23

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