CS275986B6 - The method of recovering the acid phosphatase from citroles by ultrafiltration - Google Patents

Abstract

The purpose of the solution is a method of isolating acid phosphatase from a fermented solution from the production of citric acid using ultrafiltration. This interesting enzyme has so far remained unused in citric acid liquors and in the previous process of isolating citric acid, it was destroyed and polluted citric acid and wastewater.

Description

The invention relates to a process for recovering acid phosphatase from citrils by ultrafiltration.

The name lemon juice refers to the fermented solution from citric acid production. In addition to water and organic acids, which are up to 12 to 15% by weight, the high molecular weight substances in the amount of 0.1 to 1% by weight, among which the acid phosphatase enzyme is of industrial importance, are present in the citrates aa. However, the enzymatic activity of acid phosphatase is destroyed by the present method of isolating citric acid from the citrils by precipitating it with Ca / OH / g.

This disadvantage is overcome by the process of obtaining acid phosphatase from citrons containing 100 / UKat ^-1 to 750 / UKat.1 'acid phosphatase according to the invention, which consists in separating the acid phosphatase from the solution by ultrafiltration through a membrane with a cut-off limit of 5,000 to 50 000, at a temperature of 5 to 50 ° C, at a pressure of 0.1 to 0.5 MPa, at a flow rate of psrmeate csz

A -2 -1 -1 membrane of 4 to 60 kg.m.h to form a psrmeate containing a maximum of 50 µl of acid phosphatase and a concentrate containing 1,500 µl of at least 50,000 µmol of phosphatase,

If, by ultrafiltration, isolation of citric acid e and citrils by ultrafiltration separates high-molecular substances, among them acid phosphatase, then a higher purity of the product obtained, i.e. citric acid, is obtained. In addition, acid phosphatase can be isolated from the foregoing concentrate from ultrafiltration of citrils containing concentrated high molecular weight substances. It is described that this concentrate can be used directly as a technical preparation of acid phosphatase after preservation or drying. Acid phosphatase will find use in feed preparation and other applications.

Example 1

000 parts citrons containing 100 µl at. The acid phosphatase is ultrafiltered with an ultrafiltration membrane having a cut-off of 20,000 at a pressure of 0.3 MPa and a temperature of 20 ° C, yielding 950 parts

-2 -1 at a permissive flow rate of permeate through the membrane of 25 kg.m.h of perennial containing 5 .mu.l ^{. Of} acid phosphatase and 5 .mu.l of concentrate containing -1.

1950, uKat.1 acid phosphatase.

Claims (1)

PATENT CLAIMS Process for recovering acid phosphatase from citrons containing 100 µg / liter of acid phosphatase, characterized in that the acid phosphatase from the solution is separated by ultrafiltration through a membrane with a cut-off limit of 5 000 to 50 000, at a temperature of 5 to 50 ° C, at a pressure of 0.1 to 0.5 MPa, at a permian flow rate of 4 to 60 kg · m · h · h to produce a parmaate containing a maximum of 50 uK · ¹ · ¹ · ¹ · acid phosphatase and a concentrate containing 1,500 [mu] Kat -1 to 50 [mu] O / kKat -1 acid phosphatase.