CS269320B1 - Blistered inoculum smears - Google Patents

Abstract

The solution concerns a prophylactic inoculation solution for ruminants containing lyophilized strains of bacteria of the genera Rualnocoocua albua, Butyrivibrlo flbrisolvone, Bacteroidea ruainieola, Salanooonaa rumlnantiua, Eubaetariua llaoaua and Megaephaara oladonll, which is applied to calves up to 14 days of age. This solution induces a typical buchorian fermentation of calves during short-term application, enables an easier transition of animals to a plant diet and increases weight gain in young ruminants.

Description

The invention relates to a probiotic inoculation composition for ruminants.

Probiotloc inoculation preparations enable the development of a stable baohor ecosystem of young even if the young are separated from the mother.

No special probiotics have been developed for ruminant pups and the same microorganisms are used for them as for monogastres, which, however, often do not show the same effect in ruminants, especially in older pups. Therefore, ruminants must be inoculated with cultures of bacteria that survive in a slip ecosystem. These are mostly lactic acid-producing bacteria, which significantly lowers the pH of the environment, thus preventing the multiplication of most pathogens and allowing the growth of the necessary microorganisms. Representatives of the genera Lactobacillus and Streptococcus / Kmei V. et al., Vet. Copper. 32. 705708, 1987 »Gilliland C., T. Dalvy Sol, 62» 1980 /.

In addition to microbial probiotics, preparations containing lyophilized or dried slurry fluid containing living microorganisms are used (Ziolecki A., Kwiatkowska £., Laskowska H., 1984 - The effect of stabilized rumen extract on growth and development of calves. 2. Digestive activity In the rumen and development of mioroflora in the rumen and faeoes. 3. Tlerphys. Tiererndhr.u. Puttermittel., 51/1 /, 20-31 / These preparations are intended to induce a typical rumen fexmentation.

The disadvantage of milk-type fermentation preparations is the limited duration of action and unbalanced results in repeated experiments / Johnsson Eva, Olsson I., 1985 - The effect on performance, health and faecal microflora of feeding lactobacillus strain to neonatal calves. Swedish. J. agric. Res., 15, 71-76 /.

In addition, the strains used slow down the formation of a typical Baohor fermentation in the emerging foregutters. These disadvantages are eliminated by the use of a probiotic inoculation mixture of baehoric bacteria for ruminants according to the invention.

The probiotic inoculation mixture consists of lyophilized strains of baehoric bacteria Ruminoooccus albus, Butyrivibrio fibrlsolvens, Bacteroldes rumlnlcola, Selenomonas rumlnantium, Enbacterium limosum and Megasphaera elsdenii. As an inoculum culture, it is possible to use an isolate having the main properties of said species. The total number of bacteria in the preparation ranges from 10 to 10 bacteria / g of the mixture and the content of all individual strains ranges from 3 to 30% of the total number. The wide range is given by the number of vital bacteria in the lyophilized preparation.

Prior to inoculation, the suspected bacterial preparation is preferably mixed with milk powder and resuspended in warm water at about 40 ° C. It is administered to the pups at the root of the tongue. Inoculation is performed on pups up to 14 days of age at 10 to 10 ⁸ bacteria / piece / dose and repeated at least twice more at three-day intervals, which makes it easier for the animal to switch to a plant diet and increase weight gain in ruminant pups. After tripling, an increase in calf growth of 9 to 16% and a reduction in diarrhea were already observed.

The advantage of the solution according to the invention is the creation of a standard preparation of a mixture of baehor bacteria. Selected strains of bacteria perform all the main functions of the rumen population and metabolically follow each other. The main advantage is the possibility of inducing a typical rumen phenomenon 1 during short-term application. In all cases, the preparation increased the growth of the animals, as evidenced by the following example of a specific application of the subject invention.

Example

The effect of the inoculum mixture was tested on calves aged 2 to 14 days after birth. The calves were given a mixture of bacteria in an amount of 10? bacteria / calf / day /5,7.10? elsdenii, 371.10? E. limosum, 2,3.10? R. albus, 4 * 10? 8. rumlnantlum, 6.10? B. rttmlnleola and 0.4.10? B. fibrisolvens /. The lyophilized bacteria were mixed with milk powder in a ratio of 1: 1 and reuspended in warm water. The mixture was applied with a syringe to the root of the tongue, the inoculation was repeated twice after two days. The results of both experiments are in Table 1.

CS 269 320 Bl

The mixture of bacteria increased the growth of calves, significantly if they were up to ten days old. When administered to older calves, the positive effect is reduced and the increase in growth is not significant.

Table 1

Influence of inoculations of calves during the period of dairy nutrition on their weight gain

inoculum number of calves age of calves at inotalaoi / days - 0 / calf weight during inoculation! / kg - 0 / monitoring period / days / weight gain of calves / g / day / -0 note I. experiment October 1987 mixture of bacteria 6 5.8 t 4.5 34.8 t 6.6 31 677 and 31.6 ^{1 2} 9.2% increase control 6 5.8 t 3.1 41.0 t 3.8 31 620 t 26.8 against control II. experiment April 1988 mixture of bacteria 8 9.9 6.9 42.6 t 3.4 20 645 - 122 16% - control 8 6.9 and 5.4 40.3 - 5.0 20 566 - 84 increase against control

- The inoculum was composed of · six strains of anaerobic bacteria isolated from the rumen of ruminants / 5,7,10? elsdenii, 371.10? B. limosum, 2,3.10? R. albus, 4.10?

S. ruminantium, 6.10? B. ruminioola a 0,4.10? B, fibrisolvens /

- difference from control at significance level P 0.05 / P statistical significance /

Claims (1)

PREVENT THE DISCOVERY Probiotic inoculation mixture for ruminants, characterized in that it contains lyophilized bacteria of the strains Ruminooooous album · * Butyrivibrio fibrisolvens, Bacteroides ruminioola, Selenomonas ruminantium, Eubaoterlum limosum and Megasphaera elsdenii in an amount of 10? to 10% bacteria / g of mixture, the individual strains being present in the mixture in the range of 3 to 30% of the total number.