CS268592B1 - Method of slime illustration in paraffin cuts - Google Patents
Method of slime illustration in paraffin cuts Download PDFInfo
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- CS268592B1 CS268592B1 CS88592A CS59288A CS268592B1 CS 268592 B1 CS268592 B1 CS 268592B1 CS 88592 A CS88592 A CS 88592A CS 59288 A CS59288 A CS 59288A CS 268592 B1 CS268592 B1 CS 268592B1
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- water
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000012188 paraffin wax Substances 0.000 title claims abstract description 9
- 239000002244 precipitate Substances 0.000 claims abstract description 3
- 239000002253 acid Substances 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 11
- 210000003097 mucus Anatomy 0.000 claims description 8
- 239000000975 dye Substances 0.000 claims description 6
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 claims description 5
- MPVDXIMFBOLMNW-ISLYRVAYSA-N 7-hydroxy-8-[(E)-phenyldiazenyl]naphthalene-1,3-disulfonic acid Chemical compound OC1=CC=C2C=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1\N=N\C1=CC=CC=C1 MPVDXIMFBOLMNW-ISLYRVAYSA-N 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000004214 Fast Green FCF Substances 0.000 claims description 3
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 claims description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 3
- 238000004043 dyeing Methods 0.000 claims description 3
- 235000019240 fast green FCF Nutrition 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 239000008096 xylene Substances 0.000 claims description 3
- DCYGAPKNVCQNOE-UHFFFAOYSA-N 2,2,2-triphenylacetic acid Chemical compound C=1C=CC=CC=1C(C=1C=CC=CC=1)(C(=O)O)C1=CC=CC=C1 DCYGAPKNVCQNOE-UHFFFAOYSA-N 0.000 claims 1
- 235000007173 Abies balsamea Nutrition 0.000 claims 1
- 239000004857 Balsam Substances 0.000 claims 1
- 244000018716 Impatiens biflora Species 0.000 claims 1
- 239000000980 acid dye Substances 0.000 claims 1
- 230000002378 acidificating effect Effects 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000004927 clay Substances 0.000 abstract 4
- PURJGKXXWJKIQR-UHFFFAOYSA-N 4-[(4-hydroxynaphthalen-1-yl)diazenyl]benzenesulfonic acid Chemical compound C12=CC=CC=C2C(O)=CC=C1N=NC1=CC=C(S(O)(=O)=O)C=C1 PURJGKXXWJKIQR-UHFFFAOYSA-N 0.000 abstract 1
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- QDLAGTHXVHQKRE-UHFFFAOYSA-N lichenxanthone Natural products COC1=CC(O)=C2C(=O)C3=C(C)C=C(OC)C=C3OC2=C1 QDLAGTHXVHQKRE-UHFFFAOYSA-N 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 239000012192 staining solution Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 2
- 230000000762 glandular Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- ZYECOAILUNWEAL-NUDFZHEQSA-N (4z)-4-[[2-methoxy-5-(phenylcarbamoyl)phenyl]hydrazinylidene]-n-(3-nitrophenyl)-3-oxonaphthalene-2-carboxamide Chemical compound COC1=CC=C(C(=O)NC=2C=CC=CC=2)C=C1N\N=C(C1=CC=CC=C1C=1)/C(=O)C=1C(=O)NC1=CC=CC([N+]([O-])=O)=C1 ZYECOAILUNWEAL-NUDFZHEQSA-N 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 238000011887 Necropsy Methods 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000004836 empirical method Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- INCIMLINXXICKS-UHFFFAOYSA-M pyronin Y Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2OC3=CC(N(C)C)=CC=C3C=C21 INCIMLINXXICKS-UHFFFAOYSA-M 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 229950003937 tolonium Drugs 0.000 description 1
- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
- AAAQKTZKLRYKHR-UHFFFAOYSA-N triphenylmethane Chemical group C1=CC=CC=C1C(C=1C=CC=CC=1)C1=CC=CC=C1 AAAQKTZKLRYKHR-UHFFFAOYSA-N 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 239000001018 xanthene dye Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Rleienle sa týká sposobu znázorněni# hlíenu v obore h1stologlckejtechniky, ktorá ráznými farb1adm1 metodami znázorňuje biologická Struktúry v živých 1 neživých objektech v rámci medicíny a prlrodnýeh vledj Očelom rleienle je dokázat přítomnost hlíenu v biologickou materiál!· Podstatou rleáenla je ofarbenie hlíenu v parafinových rezoch v prvej fáze pomocou kyslých farblv trlfenylmethanovej skupiny, v druhej fáze farblvom oranže S a kyseliny fosfovolfrámovej, ktorá vyzráža hllen a v tretej fáze sa farbl pomocou farblv xanthenových. Sposob znázorntnla hlíenu a hllenovltých látok v parafinových rezoch uvedenou metodou má možnost použltia v bloptlckej a nekroptlckej praxi a pri h1atolog!ckej výuibe itudentov medicíny.Rleienle refers to the method shown # clay in the field, which by vigorous farb1adm1 methods shows the biological structures in the living 1 inanimate objects within medicine and prlrodnýeh vledj rleienle is to prove them presence of clay in biological The material! clay in paraffin sections in the first phase using acidic farblv trenylphenylmethane group, in the second phase farblvom orange S and phosphovolfic acid, which precipitates stupid and in the third phase with farbl by xanthene. The method depicts clay and dull paraffin sections by the method it can be used in bloptlck and non-clerical practice and atologist teaching of medical students.
Description
Vynález rieii farebné znázorněni· hlienu v parafinových rezoch.The invention relates to the color representation of mucus in paraffin sections.
Na dokaž hlienu a hlienovitých látok aa v patologii doposiat používali jednak »·tódy empirické ako např. farbenie aucikara1 no· a nukheaateinoa. Tieto aetody se vlak pre svoju neipecif1ckost postupné nahradili nasledovnýai histocheaickýni aetódaal. Polysacharidy sa dokazujů aetódou PAS /Periodic Acid Schiff/, při ktorej oxidádou polysacharidov vznikajů dialdehydy, ktoré reagujů so Schiffovou regenciou za vzniku červenofialového sfarbenie. Haleho aetoda využívá schopnost kyslých skupin glykoproteinov elektrostaticky viazat kolddný roztok trojaocného železa. Takto vlažené železo reaguje 3ale) s roztoko· ferrokyanidu draselného za vzniku nerozpustné) berlinskej «odrej. K 3 ali i· aetódaa patři farbenie alciánovou «odrou, ktorá je básický· farbivo· ftalocyaainového typu s koaplexne viazanýa atomoa ned1 a tiež aetoda aetachromat1ckej reakcie s toluídinovou modrou. Okrem týchto najznámejI ich metod existujů eití člalde ale váčiinou sú to nodifikácie týchto už spominaných hl stochem1ckých metodik.Until now, empirical methods such as e.g. coloring aucikara1 no · a nukheaateinoa. Due to their non-specificity, these methods were gradually replaced by the following histocheactic method. Polysaccharides are detected by the PAS method (Periodic Acid Schiff), in which the oxidation of polysaccharides produces dialdehydes which react with Schiff regency to produce a red-violet color. Hale's method uses the ability of acidic groups of glycoproteins to electrostatically bind a cold solution of ferric iron. The iron thus moistened reacts 3ale) with a solution of potassium ferrocyanide to form an insoluble Berlin abrasion. The method includes staining with an alcian abrasion, which is a basic dye of the phthalocyanine type with coaplexed bonded atoms, as well as the method of an aetachromatic reaction with toluidine blue. In addition to these most well-known methods, there are a number of members, but the main reason is the nodifications of these already mentioned capitalist methodologies.
Podstatou vynálezu je znázornenie hlienu v parafinových rezoch na.bázi postupného odparafinovania řezu obsahujúceho hUen v organických rozpúlladlách klesajůce) koncentrácie s následný· znázorněním hi stologickýai farbivaai tým sposobo·, že odparafinovaný rez sa ponechá v destilováno) vodě po dobu 3 až 5 «in a za tý« preparát zbavíme vody týa sposobo·, že ho necháme votne vysullt na vzduchu po dobu 24 hodin při laboratorně) teplote. Vysuiený rez sa poto· farbi v troch za sebou nasledujůcich fázach. V prvej fázi sa farbi farbiaci· roztoko· obsahujúci· kyslé farbivá trifenylmethanovej skupiny a to 1,5 až 2,5 g Fast Green FCF, 50 g destilovanej vody, 50 g 96 X alkoholu a 3,5 až 4,5 g kyseliny fosfowolfráaovej po dobu 5 až 15 ain. Po ofarbeni sa rez opláchne vo vodovodně) vodě a v druhé) fáze sa farbi roztoko· skladajůcim sa z 3 až 5 g kyseliny fosfowolf ránovej a z 0,5 až 1 g oranže G rozpustenej v 100 g destilovanej vody, ktorý· sa hlien zráža 2 až 3 s a po opláchnuti řezu vo vodovodnej vodě sa v tretej fáze farbi farblada roztoko· skladajůci· sa z 0,25 až 0,5 g xanthenových farbív rozpuštěných v 100 g destilované) vody po dobu 1 až 2 s a za tým sa rez opláchne vo vodovodně) vodě, nechá votne uschnůt na vzduchu a namočí sa do xylénu, kde sa ponechá po dobu 5 až 10 ·1η. Za tým ta raz převrstvi médioa, například kanadský· balzaaoa rozpustnýa v xyléne.The object of the invention is to show the mucus in paraffin sections on the basis of the gradual dewaxing of a section containing hen in organic solvents of decreasing concentration, followed by a representation of the heterologous dyes in such a way that the dewaxed section is left in distilled water for 3 to 5 minutes. for the preparation, dehydrate the preparation in such a way that it is allowed to air dry for 24 hours at room temperature. The dried section is stained in three successive stages. In the first phase, a dyeing solution containing acidic dyes of the triphenylmethane group is dyed, namely 1.5 to 2.5 g of Fast Green FCF, 50 g of distilled water, 50 g of 96 X alcohol and 3.5 to 4.5 g of phosphotungstic acid. for 5 to 15 ain. After staining, the section is rinsed in tap water and in the second phase it is stained with a solution consisting of 3 to 5 g of phosphotungstic acid and 0.5 to 1 g of orange G dissolved in 100 g of distilled water, which precipitates from 2 to 5 g of 3, after rinsing the cut in tap water, in the third phase the dye is dyed a solution consisting of 0.25 to 0.5 g of xanthene dyes dissolved in 100 g of distilled water for 1 to 2, after which the cut is rinsed in the tap ) in water, allowed to air dry and soaked in xylene, where it is left for 5 to 10 · 1η. This is followed by overlaying a medium, such as Canadian xylene-soluble balzaaoa.
Výhoda vynálezu spočívá v značné) časovej úspoře vočl predchádzajůcia sposobo· farbenia, rýchla a nenáročná příprava farbiadch roztokov, dlhodobá trvanlivost ofarbených preparátov, dobrý farebný kontrast a táto aetoda prispleva k áaliienu objasňovaniu vlastnosti hlienu a hlienovitých látok.The advantage of the invention lies in the considerable time saving of the prior staining process, fast and undemanding preparation of color solutions, long-term shelf life of the stained preparations, good color contrast and this method contributes to the elucidation of the properties of mucus and mucus.
Přiklad prevedeniaExemplary embodiment
Ako materiál obsahujúci hUen sa použije exdzia z humánně) stinné) žtazy glandula submand 1 bulár1s, ktorá sa fixuje 4 X foraaIdehydo·. Z parafinového řezu sa odstráni parafin pomocou organických rozpůštadiel klesajúcej koncentráde a ponechá sa v destilované) vodě po dobu 2 až 3 min. Za týa zbavíme preparát vody týa sposobo·, že ho necháme votne vysullt na vzduchu po dobu 24 hodin pri laboratorně) teplote. Vysulený rez sa potom farbi v troch za sebou nasledujůcich fázach. V prvej fázi sa ponori do sklenenej kyvety obsahujúcej farbiaci roztok skladajůci sa z 1,5 až 2,5 g Fast Green FCF, 50 g destilované) vody, 50 g 96 X alkoholu a z 3,5 až 4,5 g kyseliny fosfowolfráaovej po dobu 5 až 15 min. Po ofarbeni sa rez opláchne vo vodovodně) vodě a ponori do áatlej kyvety obsahujúce) farbiaci roztok pozostávajúci z 3 až 5 g kyseliny fosfowolfránovej a z 0,5 až 1 g oranže G rozpuštěné) v 100 g destilované) vody po dobu 2 až 3 sec. Po uvedene) době sa vykoná dokladný, ale nie dlhý oplach vo vodovodně) vodě a rez sa ponori do datle) kyvety obsahujúce) farbiaci roztok, ktorý pozostáva z 0,5 g Pyroninu G rozpuštěného v 100 g destilované) vodě, kde sa farbi po dobu 1 až 2 sec. Znova sa vykoná krátký oplach vo vodovodně) vodě a nechá sa rez votne usulit na vzduchu. Po jehoThe hUene-containing material used is an extract from the human glandular glandular submand 1 bular, which is fixed with 4 x formaldehyde. The paraffin is removed from the paraffin section using organic solvents of decreasing concentration and left in distilled water for 2 to 3 minutes. In this case, we remove the water preparation in such a way that it is allowed to air dry for 24 hours at room temperature. The salted section is then stained in three successive stages. In the first stage, immerse in a glass cuvette containing a staining solution consisting of 1,5 to 2,5 g of Fast Green FCF, 50 g of distilled water, 50 g of 96% alcohol and 3,5 to 4,5 g of phosphotungstic acid for 5 to 15 min. After staining, the section is rinsed in tap water and immersed in an oil cuvette containing a staining solution consisting of 3 to 5 g of phosphotungstic acid and 0.5 to 1 g of orange G dissolved in 100 g of distilled water for 2 to 3 seconds. After this time, a thorough but not long rinse in tap water is performed and the section is immersed in a date cuvette containing a staining solution consisting of 0,5 g of Pyronin G dissolved in 100 g of distilled water, where it is stained after for 1 to 2 sec. Perform a short rinse in tap water again and allow the cut to settle in air. After his
CS 268 592 B1 zaschnuti sa ponor! do kyvety obsahujúcej xylén, ponechá sa po dobu 5 až 10 min v nej a óalej sa zakrývá do médii rozpustných v xyléne. Výsledok farbenia je nasledovný: hllenové mudnózne tubuly stinných žllaz «ů výraznej ružovof1 alovej farby na zelenom pozadí. Oobré výsledky sů aj pri ofarbeni tráviacej a dýchacej sůstavy.CS 268 592 B1 dry dry! into a cuvette containing xylene, left in it for 5 to 10 minutes and then covered in xylene-soluble media. The result of the staining is as follows: mucous mucous tubules of shady glands of a distinctive pink-violet color on a green background. Good results are also obtained with discoloration of the digestive and respiratory system.
Sposob znázornenla hllenu a hllenovltých létok v parafinových rezoch uvedenou metodou má možnost použitia v bioptickej a nekroptickej praxi na oddeleniach patologie - a pri histologickej výuíbe ítudentov medicíny.The method of depicting mucus and mucous yellows in paraffin sections by the above method has the possibility of use in biopsy and necropsy practice in the departments of pathology - and in the histological teaching of medical students.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS88592A CS268592B1 (en) | 1988-02-01 | 1988-02-01 | Method of slime illustration in paraffin cuts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS88592A CS268592B1 (en) | 1988-02-01 | 1988-02-01 | Method of slime illustration in paraffin cuts |
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| Publication Number | Publication Date |
|---|---|
| CS59288A1 CS59288A1 (en) | 1989-08-14 |
| CS268592B1 true CS268592B1 (en) | 1990-03-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS88592A CS268592B1 (en) | 1988-02-01 | 1988-02-01 | Method of slime illustration in paraffin cuts |
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| Country | Link |
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| CS (1) | CS268592B1 (en) |
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1988
- 1988-02-01 CS CS88592A patent/CS268592B1/en unknown
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| Publication number | Publication date |
|---|---|
| CS59288A1 (en) | 1989-08-14 |
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