CS268592B1

CS268592B1 - The method illustrates clay in paraffin sections

Info

Publication number: CS268592B1
Application number: CS88592A
Authority: CS
Inventors: Katarina Tothova
Original assignee: Katarina Tothova
Priority date: 1988-02-01
Filing date: 1988-02-01
Publication date: 1990-03-14
Also published as: CS59288A1

Abstract

Rleienle sa týká sposobu znázorněni# hlíenu v obore h1stologlckejtechniky, ktorá ráznými farb1adm1 metodami znázorňuje biologická Struktúry v živých 1 neživých objektech v rámci medicíny a prlrodnýeh vledj Očelom rleienle je dokázat přítomnost hlíenu v biologickou materiál!· Podstatou rleáenla je ofarbenie hlíenu v parafinových rezoch v prvej fáze pomocou kyslých farblv trlfenylmethanovej skupiny, v druhej fáze farblvom oranže S a kyseliny fosfovolfrámovej, ktorá vyzráža hllen a v tretej fáze sa farbl pomocou farblv xanthenových. Sposob znázorntnla hlíenu a hllenovltých látok v parafinových rezoch uvedenou metodou má možnost použltia v bloptlckej a nekroptlckej praxi a pri h1atolog!ckej výuibe itudentov medicíny.Rleienle refers to the method of representation# of mucus in the field of histology, which shows biological structures in living and non-living objects within the framework of medicine and birth science using vivid color methods. The purpose of rleienle is to prove the presence of mucus in biological material! The essence of rleienle is the staining of mucus in paraffin sections in the first phase using acid dyes of the trlphenylmethane group, in the second phase with the dye orange S and phosphotungstic acid, which precipitates hlen, and in the third phase it was dyed using xanthene dyes. The method of illustrating mucus and mucus-like substances in paraffin sections with the above method has the possibility of being used in pathology and necropsy practice and in the haematological education of medical students.

Description

CS 268 592 B1CS 268 592 B1

Vynátez řítil farebné znázorněni· bílenu v parafinových rezoch.The inventor has driven a colored representation of the white in the paraffin sections.

Na dokaž hllenu a hllenovltých látok aa v patologii dopoalat používali Jednak me-tody empirické ako napr. farbenle aucikarm1noa a mukheaate1noa. Tleto metody ae vlakprt avoju neipeclfIckoat poatupne nahradili naaledovnýal hlstochemlckýml metodami. Po-lysacharldy aa dokazujů metodou PAS /Perlodlc Ac1d Schlff/, pr1 ktorej oxldéclou poly-aacharldov vznlkajú dlaldehydy, ktoré reagujú ao Schlffovou regenclou za vzniku červe-noflalového afarbenla. Kalého metoda využívá achopnoat kyslých skupin glykoprotelnovelektrostaticky vlazat kololdný roztok trojmocného železa. Takto vlažené železo reagujedále] a roztokom ferrokyanldu draselného za vzniku nerozpuatnej berlínské] modrej. K dalilm metodám patři farbenle alclénovou modrou, ktoré je béalckým farblvom ftalocya-mlnového typu a komplexně vlaženým atómom médi a t1ež metoda metachromat1cke] reakclea toluldlnovou modrou. Okrem týchto na]známe]i 1ch metod exlstujú eité dalile ale víčil-nou sú to modlflkéde týchto už spomlnaných hlstochem1ckých metodik.Empirical methods, such as aucikarm1noa and mukheaate1noa, were used to prove dull and dull substances and in the pathology dopoalat. These methods and the train and development of neipeclfIckoat have been replaced by naaledovll's methods. The polysaccharide and the PAS / Perlodlc Acld Schlff®, whose polyaccharide oxides are formed by the palladium which reacts and by Schloff's reagent to form a red-violet afarbene. The muddy method uses the achopnoat of acidic groups to glycoprotelnovelectrostatically melt the colloidal solution of trivalent iron. The so-iron iron reacts with the solution of potassium ferrocyanide to form an insoluble Berlin blue. Other methods include the blue-stained dye, which is the Béphal color of the phthalocyanine-type and the complexly lukewarm atom of the medium, and the metachromate method, and the blue-blue dye. In addition to these well-known methods, eile dalile, but more or less these methods are already mentioned.

Podatatou vynálezu je znázornenle hllenu v parafinových rezoch na.bázi postupnéhoodparafInovanla řezu obaahujúeeho hHen v organických rozpúiladlách klesajúee] končen- Λ tréde s následným znázorněním h1stolog1ckým1 farblvaml tým sposobom, že odparaf1nova-ný rez aa ponechá v destllovanej vodě po dobu 3 až 5 min a za tým preparát zbavíme vodytým sposobom, že ho necháme votne vyaullt na vzduchu po dobu 24 hodin prl laboratorně]teplote. Vytužený rez aa potom farbl v troch za sebou natledujúclch fázach. V prve]fázi aa farbl farbladm roztokom obaahujúdm kyslé farblvá trlfenylmetha novej skupinya to 1z5 až 2,5 g Past Green FCF, 50 g destllovanej vody, 50 g 96 Z alkoholu a 3,5 až 4,5 g kyseliny fosfowolfrámové] po dobu 5 až 15 min. Po ofarbenl ta rez opláchne vo vo-dovodnej vodě a v druhé] fáze aa farbl roztokom skladajůdm aa z 3 až 5 g kyseliny fos-fowolf rámové] a z 0,5 až 1 g oranže G rozpuštěné] v 100 g destllovanej vody, ktorýmaa hllen zráža 2 až 3 a a po opláchnuti řezu vo vodovodně] vodě sa v třete] fáze farblfarbladm roztokom skladajůdm sa z 0,25 až 0,5 g xanthenových farblv rozpuštěnýchv 100 g destllovanej vody po dobu 1 až 2 a a za tým sa rez opláchne vo vodovodnej vo-dě, nechá votne uschnút na vzduchu a namočí sa do xylénu, kde sa ponechá po dobu 5až 10 min. Za tým aa rez převrstvi médlom, například kanadským balzamom rozpustnýmv xyléne. Výhoda vynálezu spočívá v značné] časové] úspoře voč1 predchádza]ůc1· sposobomfarbenla, rýchla a nenáročná příprava farbladch roztokov, dlhodobá trvanlivost ofarbe-ných preparátov, dobrý farebný kontrast a táto metoda prispleva k dalilemu objasňovanluvlastnosti hllenu a hllanovltých látok. Přiklad prevedenlaAccording to the present invention there is shown a paraffin-embedded slice in a stepwise separation section comprising hHen in organic solvents, decreasing in the process followed by deprotection in a way that the dewaxed section is left in distilled water for 3 to 5 min. thus, the preparation is freed from the water in a way that leaves it in the air for 24 hours at room temperature. Rough cut and then color in three consecutive phases. In the first phase, a color solution containing acidic colorates of a new phenyl group is 1 to 2.5 g of Past Green FCF, 50 g of distilled water, 50 g of 96% alcohol, and 3.5 to 4.5 g of phosphotungstic acid. up to 15 min. After staining, the rust is rinsed in water and in the second phase and the solution is made up of 3 to 5 g of phosphosolfic acid, and 0.5 to 1 g of orange G dissolved in 100 g of distilled water. precipitation of 2 to 3 and, after rinsing the cut in tap water, in a three-phase phase, the color solution is comprised of 0.25 to 0.5 g of xanthene dyes dissolved in 100 g of distilled water for 1-2 hours, and the cut is rinsed in tap water. water, allowed to air dry in air and soak in xylene for 5 to 10 min. For this, they are cut with an overlay of a medium such as xylene-soluble Canadian balsam. The advantage of the invention lies in the considerable time savings of the colorant, the rapid and inexpensive preparation of coloring solutions, the long-term durability of the stained preparations, the good color contrast and this method to further elucidate the properties of the clay and yellowish substances. Example transposed

Ako materiál obsahujúd hllen sa použije exdzla z humánně] sllnnej iíazy glandu-la aubmand1 bulár1s, ktorá aa fixuje 4 X formaIdehydom. Z parafinového řezu aa odatránlparafin pomocou organických rozpúitadlel kleaajúeej koncentrácle a ponechá aa v destilova-né] vodě po dobu 2 až 3 min. Za tým zbavíme preparát vody tým apoaobom, že ho nechámevotne vyaullt na vzduchu po dobu 24 hodin pr1 laboratorně] teplote. Vysuiený rez aa po-tom farbl v troch za sebou nasledujůdch fázach. V prve] fázi sa ponoří do sklenene]kyvety obaahujúce] farblacl roztok skladajúd sa z 1,5 až 2,5 g Fast Green FCF, 50 gdestllovanej vody, 50 g 96 X alkoholu a z 3,5 až 4,5 g kyseliny fosfowolfrámové] po do-bu 5 až 15 min. Po ofarbenl sa rez opláchne vo vodovodně] vodě a ponoří do SaCiej ky-vety obaahujúce] farblacl roztok pozoatávajúd z 3 až 5 g kyseliny fosfowolfrámoveja z 0,5 až 1 g oranže G rozpuštěna] v 100 g destllovanej vody po dobu 2 až 3 sec.The material containing the clavium is exdzela from a human] lipase glandu-laubmand1 bullus, which is fixed with 4 X form aldehyde. From the paraffin section aa paraffin is removed by means of organic solvents to leave a concentrate and left in a distilled water for 2-3 min. For this purpose, the water preparation is freed from the water so that it is able to heat it in the air for 24 hours at room temperature. The dried rust and after-color blend in three consecutive stages. In the first phase, it is immersed in a glass cuvette containing a color solution consisting of 1.5 to 2.5 g of Fast Green FCF, 50 g of distilled water, 50 g of 96 X alcohol and 3.5 to 4.5 g of phosphotungstic acid] after 5 to 15 min. After staining, the incision is rinsed in tap water and immersed in a SaiCyi cuvette containing the coloring solution consisting of 3-5 g of phosphotungstic acid and 0.5 to 1 g of orange G dissolved in 100 g of distilled water for 2-3 sec. .

Po uvedenej době ta vykoná dokladný, ale nle dlhý oplach vo vodovodně] vodě a rez aaponoří do datle] kyvety obsahujúcej farblacl roztok, ktorý pozoatáva z 0,5 g Pyronlnu Grozpuštěného v 100 g destllovanej vodě, kde aa farbl po dobu 1 až 2 sec. Znova aa vy-koná krátký oplach vo vodovodnej vodě a nechá aa rez votne usulit na vzduchu. Po JehoAfter this time, it performs a rugged but long rinse in tap water and cuts into a date cuvette containing a color solution consisting of 0.5 g of Pyronene G dissolved in 100 g of distilled water, where aa farbl for 1 to 2 sec . Again and again, he performs a short rinse in tap water and lets and cuts in the air. After His

Claims

* PREDICTION OF THE INVENTION

A method of depilating mucus in paraffin sections based on the gradual dewaxing of a section containing mucus in organic solvents of decreasing concentration and subsequent representation, for example with histological dyes, characterized in that the dewaxed section is left in distilled water for 3 to 5 seconds. by allowing it to air dry for 24 hours at room temperature and then dyeing it in three successive stages, the first stage being dyeed with triphenylethanoic acid dyes, namely 1.5 to 2.5 g of Fast Green FCF, 50 g of distilled water, 50 g of 96 X alcohol and 3.5 to 4.5 g of phosphotungstic acid for 5 to 15 minutes, after which, after rinsing with water, it is colored in a second phase with a solution consisting of 3 to 5 g of acid. of phosphotransphane and 0,5 to 1 g of orange G dissolved in 100 g of water distilling water, which precipitates the mucus for 2 to 3, and, after rinsing the cut in water, is dyed in the third phase with a dye solution consisting of 0,25 to 0, 5 g xant hen dyes dissolved in 100 g of distilled water, after 1 to 2 s of dyeing, the section is rinsed with water, allowed to air dry, immersed in xylene for 5 to 10 minutes. behind it, a medium of, for example, Canadian xylene-soluble balsam is overlaid. ^r