CS265510B1 - A method of isolating chemically pure amino acids from a mixture thereof - Google Patents

Abstract

The purpose of isolating amino acids from their mixture is to achieve a purity of 99.99% using waste-free technology, with minimal equipment and energy costs. The method of isolating amino acids, especially from fermentation media, hydrolysates and waste from amino acid production, consists in desalting the solution of the amino acid mixture, adjusting its conductivity to values from 100 to 1,000/uS.cm and autofocusing for 24 to 48 hours. The individual amino acids obtained in fractions with a pH close to their isoelectric point are further separately autofocused. Subsequent refocusings, the number of which depends on the required purity of the amino acids, produce parallel chemically pure amino acids. Finally, the amino acids are dehydrated. The procedure has applications in the chemical and pharmaceutical industries, food processing, agriculture, research and diagnostics.

Description

The invention relates to a process for the isolation of chemically pure amino acids from their mixture for the needs of the pharmaceutical industry, research and diagnostics.

Amino acids are industrially prepared by most biosynthesis using microorganisms that produce one or more amino acids into the culture medium. The amino acids are then recovered from the medium by costly isolation in the desired purity. Rarely are protein wastes, hydrolysates, broths, extracts and others used to isolate amino acids, although they often contain amino acids that do not produce any micro-organisms and therefore need to be artificially synthesized, which disproportionately increases their cost.

Common disadvantages of these methods are high equipment costs, complex handling and low yield.

The above-mentioned disadvantages are substantially eliminated by the method of isolation of chemically pure amino acids from their mixture according to the invention, which consists in the fact that the solution of the amino acid mixture is desalted, its conductivity is adjusted to 100 to 1000 / uS.cm ^-1 and autofused at a voltage of 200 to 1000 V for 24 to 48 hours and the individual amino acids found in fractions with a pH close to their isoelectric point are separately refocused and then dehydrated.

An advantage of the invention is the low cost of equipment and energy. The method of production is very simple and requires at least the demands on the operator.

Example 1

The casein hydrolyzate solution is desalted, preferably by goal filtration, its conductivity is adjusted to values from 100 to 1000 µS.cm and autofocused for 48 hours. Each amino acid is focussed from the solution to its isoelectric point. Of these occurring in the highest proportion, arginine at pH 10.76 focuses on glycine to pH 5.97 and alanine at pH 6.0. Fractions with pH close to the isoelectric point of each amino acid are collected separately from each initial focus and then applied to a smaller volume apparatus where they are refocused, thereby collecting a very pure amino acid at each pH described. Refocusing may be repeated as required for amino acid purity. Subsequently, the obtained amino acids are lyophilized. This yields up to 6.3 g.l of hydrolyzate base solution. For autofocusing, a direct current of 200 to 1000 V and a power of 3 W is used.

Example 2

The waste solution from the production of chemically pure L-lysine containing the remaining amino acid valines, alanine and histidine is autofocused according to Example 1. Valine is collected at pH 5.48, alanine at pH 6.00 and histidine at pH 7.64. For higher purity, each amino acid is autofocused further separately. Thus, a yield of 9.8 g.l of a basic solution of the amino acid mixture is achieved. Subsequently, the obtained amino acids are spray dried in a dryer.

The invention can find a wide industrial application in the chemical and pharmaceutical industries in the production of amino acids by waste-free technology.

Claims (1)

Method of isolating chemically pure amino acids from their mixture, in particular from fermentation media, hydrolysates and waste from amino acid production, characterized in that the solution of the amino acid mixture is desalted, its conductivity is adjusted to values from 100 to 1000 µS.cm and autofocused by direct current at a voltage of 200 to 1000 V at pH near the isoelectric point of the individual amino acids, for 24 to 48 hours, and the individual amino acids found in fractions with a pH near their isoelectric point are again autofocused at least once and subsequently dehydrated.